Difference between revisions of "Team:CCU Taiwan/Composite Part"

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   });
 
   });
 
});
 
});
−
var s = skrollr.init({
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// The function actually applying the offset
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render: function(data) {
+
function offsetAnchor() {
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  //Log the current scroll position.
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  if (location.hash.length !== 0) {
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  $('#info').text(data.curTop);
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    window.scrollTo(window.scrollX, window.scrollY - 90);
−
}
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  }
−
});;
+
}
 +
 
 +
// Captures click events of all <a> elements with href starting with #
 +
$(document).on('click', 'a[href^="#"]', function(event) {
 +
  // Click events are captured before hashchanges. Timeout
 +
  // causes offsetAnchor to be called after the page jump.
 +
  window.setTimeout(function() {
 +
    offsetAnchor();
 +
  }, 0);
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});
 +
 
 +
// Set the offset when entering page with hash present in the url
 +
window.setTimeout(offsetAnchor, 0);
 +
 +
$(document).scroll(function () { // remove "$"
 +
    var y = $(this).scrollTop();       
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    if (y > 300) {
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        $('.indicator').fadeIn();
 +
    }
 +
    else {
 +
        $('.indicator').fadeOut();
 +
    }
 +
});
 
 
 
</script>
 
</script>

Revision as of 21:09, 14 October 2018

Composite Parts

BBa_K2809001
Kozak sequence (Pichia pastoris)

The Kozak sequence initiates the translation in Pichia pastoris. This Sequence is copied directly from pCore1 in reference .The start codon is not included.

BBa_K2809002
Prepro-alpha-factor (codon modified for p.pastoris)

Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs secreted expression of the recombinant protein, therefore, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum(ER) lumen, this secretion signal will be cleaved.

BBa_K2809010
Mature Lac1 (laccase)

This is the codon-optimized version of laccase derived from Pinus taeda(Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation.It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.

The signal peptide have removed, so the sequence do NOT contain start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.

BBa_K2809020
Mature Px18 (peroxidase)

BBa_K2809030
Mature Px16 (peroxidase)

Those are the codon-optimized version of peroxidase derived from Picea abies(Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.

The signal peptide have removed, so the sequence do NOT contain start codon.Moreover, it includes V5-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.

The signal peptide have removed, so the sequence do NOT contain start codon.Furthermore, it includes FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.


Best New Composite parts:

BBa_K2809011
laccase 1

We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809010.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of laccase gene sequence, HA tag for Western Blot protein detection and 6xHis-tag for protein purification.

BBa_K2809021
peroxidase 18

We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809020.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 18 gene sequence, V5-tag for Western Blot protein detection and 6xHis-tag for protein purification.

BBa_K2809031
peroxidase 16

We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809030.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification.