Difference between revisions of "Team:Nanjing-China/Demonstrate"

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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li></ul></div>
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      <ul>
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      <li><a href="#overview">Overview</a></li>
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      <li><a href="#cds">CdS</a></li>
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      <li><a href="#nit"><font size="-1">Nitrogen fixation</font></a></li>
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      <li><a href="#device">Device</a></li>
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        <ul>
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  <li><a href="https://2018.igem.org/Team:Nanjing-China">PEOPLE</a>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Team</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges">For_Judges</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">PROJECT</a>
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    <ul>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Hardware">Hardware</a></li> 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improve">Improve</a></li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">MODEL</a>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">PRACTICES</a>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices"><font size="-1">Human_Practices</font></a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">NOTEBOOK</a></li>
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      <h2>Overview</h2>
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        <p>This year, we found  an economic, efficient and environmental-friendly way to utilize nitrogen in  the air. Our design consists of three parts: biosynthesis of CdS semiconductor, light-driven  nitrogen fixation and reaction device. All three parts have gone  through in-depth examination and successfully work out under real conditions.<br />
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        The following list exhibits our key  achievements in this project.<br />
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        Constructed and  tested engineered <em>E. coli</em> which express nitrogenase and OmpA-PbrR. <br />
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        Constructed and  tested light-driven system based on OmpA-PbrR protein.<br />
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        Achieved  light-driven nitrogen fixation.<br />
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        Designed a reaction  device that cater for our light-driven nitrogen fixation reaction.</p>
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<div class="word" id="cds">     
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      <h3>Biosynthesis  of CdS semiconductor</h3>
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      <p>One key element  of light-driven system in our design is CdS semiconductor precipitated by the OmpA-PbrR  fused protein. We used TEM-EDX analysis to characterize CdS semiconductor  precipitated on E. coli cell. We also determined the maximum concentration of  Cd2+ appropriate for strain growth, as well as the amount of CdS  that can be precipitated on cell surface.</p>
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        <div class="word" id="nit">
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      <h3>Light-driven  nitrogen fixation</h3>
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        <p>CdS  semiconductor generate excited electrons under illumination which are then  passed to nitrogenase for nitrogen fixation. We introduce nitrogenase to <em>E.coli</em> to enable it to reduce dinitrogen to ammonia. We conducted QPCR to detect  relative transcriptional level of each <em>nif</em> gene. We also plan to optimize the structure of <em>nif </em>gene operon after modeling. Using acetylene reduce assay, we then  verify the activity of nitrogenase in our system.</p>
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      <img src="https://static.igem.org/mediawiki/2018/4/47/T--Nanjing-China--qRT-PCR.png" width="40%" />
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        <div class="word" id="device">
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      <h3>Reaction  device</h3>
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        <p>We designed a light-driven microbe reaction  device to apply our system to practical use. After a few test, we proved our  device to be quite practical. <a href="https://2018.igem.org/Team:Nanjing-China/Hardware">(see device for more details)</a></p>
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        <div class="word-note"><img src="https://static.igem.org/mediawiki/2018/c/c2/T--Nanjing-China--device-whole.jpg" width="100%" /></div>
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
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            <p>Life Science Department<br />
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              #163 Xianlin Blvd, Qixia District<br />
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              Nanjing University<br />
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              Nanjing, Jiangsu Province<br />
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              P.R. of China<br />
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              Zip: 210046
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            </p></div>
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          <div class="f-word">
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            <p><strong><u>Email</u></strong></p>
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            <p>nanjing_china@163.com</p>
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            <p><strong><u>Social media</u></strong></p>
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            <p>Twitter button: <br/>
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            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
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Revision as of 09:04, 15 October 2018

Nanjing-China2018

Overview

This year, we found an economic, efficient and environmental-friendly way to utilize nitrogen in the air. Our design consists of three parts: biosynthesis of CdS semiconductor, light-driven nitrogen fixation and reaction device. All three parts have gone through in-depth examination and successfully work out under real conditions.
The following list exhibits our key achievements in this project.
Constructed and tested engineered E. coli which express nitrogenase and OmpA-PbrR.
Constructed and tested light-driven system based on OmpA-PbrR protein.
Achieved light-driven nitrogen fixation.
Designed a reaction device that cater for our light-driven nitrogen fixation reaction.

Biosynthesis of CdS semiconductor

One key element of light-driven system in our design is CdS semiconductor precipitated by the OmpA-PbrR fused protein. We used TEM-EDX analysis to characterize CdS semiconductor precipitated on E. coli cell. We also determined the maximum concentration of Cd2+ appropriate for strain growth, as well as the amount of CdS that can be precipitated on cell surface.

Light-driven nitrogen fixation

CdS semiconductor generate excited electrons under illumination which are then passed to nitrogenase for nitrogen fixation. We introduce nitrogenase to E.coli to enable it to reduce dinitrogen to ammonia. We conducted QPCR to detect relative transcriptional level of each nif gene. We also plan to optimize the structure of nif gene operon after modeling. Using acetylene reduce assay, we then verify the activity of nitrogenase in our system.

Reaction device

We designed a light-driven microbe reaction device to apply our system to practical use. After a few test, we proved our device to be quite practical. (see device for more details)

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china