PARTS

BASIC PARTS

During this project, our team designed the following basic parts:

Coding sequences

Ribosomal binding sites (RBS)

Promoters

Terminators

and a Golden Gate adapter

COMPOSITE PARTS

During this project, our team designed the following composite parts:

PART COLLECTION

In our project we tried to repurpose a peptide communication machinery from a phage of the Gram-positive Bacillus subtilis to the cells of Gram-negative Escherichia coli, and quickly discovered that crossing the inter-species barrier in bioengineering is a challenging task. This Part Collection consists of a series of promoter variants designed to debug the mechanism of transcriptional regulation in the aimX promoter that is key to the signalling system. The Collection helped us successfully identify an internal terminator, investigate a potential anti-termination mechanism, and build a hybrid promoter that converts the native transcriptional activator (AimR) into a transcriptional repressor. When placed upstream of a reporter gene, promoters from this Part Collection showed variable transcription strengths and degrees of regulation that provided valuable insights for our project and facilitated the porting of the peptide communication machinery from B. subtilis to E. coli (BBa_K2675050 to K2675065 & BBa_K2675190 to BBa_K2675197).