Difference between revisions of "Team:CCU Taiwan/Materials"
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | 'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div> | ||
| − | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a | + | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a> |
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a | + | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a> |
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a | + | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a> |
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a | + | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a> |
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a | + | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a> |
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
<a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a> | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a> | ||
Revision as of 15:28, 15 October 2018
Materials
Antibiotics
| Name | Supplier |
| Zeocin™ (solution) | Thermo Fisher |
| Ampicillin | SIGMA |
| Chloramphenicol | SIGMA |
Antibody
| Name | Supplier |
| Anti-HA mouse Monoclonal | Yao-Hong Biotechnology |
| Anti-V5 mouse Monoclonal | Yao-Hong Biotechnology |
| Anti-Flag mouse Monoclonal | Yao-Hong Biotechnology |
Enzyme
| Name | Supplier |
| BamHI | New England BioLabs |
| AseI | New England BioLabs |
| EcoRI | New England BioLabs |
| SalI | New England BioLabs |
| AgeI | New England BioLabs |
| PstI | New England BioLabs |
| AvrII | New England BioLabs |
Equipment
| Equipment | Name | Manufacturer |
| Table Centrifuge | Centrifuge 5415R | eppendorf |
| PCR-Machine | CYCLER-25 | major science |
| PCR-Machine | SimpliAmp™ Thermal Cycler | Life technology |
| UV light box | UV light box | TOPBIO |
| Digital gel image system | DigiGel DGIS-11c | TOPBIO |
| Water bath | Water bath B206 | FIRSTEK |
| Heat block | Dry bath incubator | major science |
| Scale | PB3002-S/PH DeltaRange | Mettler Toledo |
| NanoDrop | ND-1000 UV-Vis Spectrophotometer | Marshall Scientific |
| Hot plate | Corning® hotplate stirrers | SIBATA |
| Micro-computer compact ultra pure water system | Micro-computer compact ultra pure water system PPI-185UV | Uniss |
| Vortex mixer | Vortex-Genie 2 SI-0236 | Scientific Industries |
| Multiskan | Multiskan™ FC Microplate Photometer | Thermo |
Kits
| Name | Supplier |
| Pichia Expression Kit, original kit | Biokit |
| Miniprep | Biokit |
| Gel extraction | Biokit |
Markers
| Name | Supplier |
| 1 kb DNA marker XR-LYD008-1ML | YEASTERN BIOTECH |
| DNA Gel Loading Dye (6X) | YEASTERN BIOTECH |
Media
| Media | Composition |
| LB medium | 10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
| LB medium(low salt) | 5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
| Yeast Extract–Peptone–Dextrose (YPD) Medium | 10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O |
| Standard Minimal Medium (SD) | Yeast Nitrogen Base amino acids 6.7g H2O 865ml 50% Glucose 40ml Nutrient (without Histidine) 100ml |