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+ | <div class="pointerNotebook" id="3"><a href="#ca3">Enzyme</a></div> | ||
+ | <div class="pointerNotebook" id="4"><a href="#ca4">Equipment</a></div> | ||
+ | <div class="pointerNotebook" id="5"><a href="#ca5">Kits</a></div> | ||
+ | <div class="pointerNotebook" id="4"><a href="#ca6">Markers</a></div> | ||
+ | <div class="pointerNotebook" id="5"><a href="#ca7">Media</a></div> | ||
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Revision as of 00:49, 16 October 2018
MATERIALS
Antibiotics
Name | Supplier |
Zeocin™ (solution) | Thermo Fisher |
Ampicillin | SIGMA |
Chloramphenicol | SIGMA |
Antibody
Name | Supplier |
Anti-HA mouse Monoclonal | Yao-Hong Biotechnology |
Anti-V5 mouse Monoclonal | Yao-Hong Biotechnology |
Anti-Flag mouse Monoclonal | Yao-Hong Biotechnology |
Enzyme
Name | Supplier |
BamHI | New England BioLabs |
AseI | New England BioLabs |
EcoRI | New England BioLabs |
SalI | New England BioLabs |
AgeI | New England BioLabs |
PstI | New England BioLabs |
AvrII | New England BioLabs |
Equipment
Equipment | Name | Manufacturer |
Table Centrifuge | Centrifuge 5415R | eppendorf |
PCR-Machine | CYCLER-25 | major science |
PCR-Machine | SimpliAmp™ Thermal Cycler | Life technology |
UV light box | UV light box | TOPBIO |
Digital gel image system | DigiGel DGIS-11c | TOPBIO |
Water bath | Water bath B206 | FIRSTEK |
Heat block | Dry bath incubator | major science |
Scale | PB3002-S/PH DeltaRange | Mettler Toledo |
NanoDrop | ND-1000 UV-Vis Spectrophotometer | Marshall Scientific |
Hot plate | Corning® hotplate stirrers | SIBATA |
Micro-computer compact ultra pure water system | Micro-computer compact ultra pure water system PPI-185UV | Uniss |
Vortex mixer | Vortex-Genie 2 SI-0236 | Scientific Industries |
Multiskan | Multiskan™ FC Microplate Photometer | Thermo |
Kits
Name | Supplier |
Pichia Expression Kit, original kit | Biokit |
Miniprep | Biokit |
Gel extraction | Biokit |
Markers
Name | Supplier |
1 kb DNA marker XR-LYD008-1ML | YEASTERN BIOTECH |
DNA Gel Loading Dye (6X) | YEASTERN BIOTECH |
Media
Media | Composition |
LB medium | 10 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
LB medium(low salt) | 5 g NaCl, 10 g Tryptone , 5 g yeast extract in 1 L ddH2O |
Yeast Extract–Peptone–Dextrose (YPD) Medium | 10 g yeast extract , 20 g peptone ,20g Dextrose in 1 L ddH2O |
Standard Minimal Medium (SD) | Yeast Nitrogen Base amino acids 6.7g H2O 865ml 50% Glucose 40ml Nutrient (without Histidine) 100ml |