Difference between revisions of "Team:UCopenhagen/Protocols"
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<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| − | + | <h2>Internal protocols</h2> | |
| − | + | Selfmade protocols: | |
| − | <h2><a href="https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">Transformation of bacteria</a><span style="text-decoration:underline;"></span></ | + | <h3><a href="https://static.igem.org/mediawiki/2018/1/14/T--UCopenhagen--LeakProt.jpeg">Leakiness protocol</a><span style="text-decoration:underline;"></span></h3> |
| + | <h3><a href="https://static.igem.org/mediawiki/2018/5/57/T--UCopenhagen--SLBProt.jpeg">Supported Lipid Bilayer protocol</a><span style="text-decoration:underline;"></span></h3> | ||
| + | <h3><a href="https://static.igem.org/mediawiki/2018/8/82/T--UCopenhagen--onionProt.pdf">Injection assay protocol</a><span style="text-decoration:underline;"></span></h3> | ||
| + | <h3><a href="https://static.igem.org/mediawiki/2018/8/81/T--UCopenhagen--Clone.pdf">Cloning protocol</a><span style="text-decoration:underline;"></span></h3> | ||
| + | <h2>Internal protocols</h2> | ||
| + | Protocols from other sources: | ||
| + | <h3><a href="https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">Transformation of bacteria</a><span style="text-decoration:underline;"></span></h3> | ||
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Protocol from iGEM HQs. We use LB media instead of SOC media. | Protocol from iGEM HQs. We use LB media instead of SOC media. | ||
</p> | </p> | ||
| − | < | + | <h3><a href="http://parts.igem.org/Help:Protocols/Competent_Cells">Competent cells</a></h3> |
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We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!). | We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!). | ||
</p> | </p> | ||
| − | < | + | <h3><a href="https://2018.igem.org/Team:UCopenhagen/Protocols_Cloning">Cloning of constructs</a>*</h3> |
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This protocol describes our strategy when assembling of the constructs that we worked with in lab. | This protocol describes our strategy when assembling of the constructs that we worked with in lab. | ||
</p> | </p> | ||
| − | < | + | <h3><a href="https://static.igem.org/mediawiki/2018/6/6d/T--UCopenhagen--Protocol_Liposome_preparation_step_by_step_NH_lab.pdf">Liposomes preparation step-by-step</a></h3> |
<p> Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.</p> | <p> Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.</p> | ||
| − | < | + | <h3><a href="https://static.igem.org/mediawiki/2018/5/5b/T--UCopenhagen--Protocol_Liposome_preparation_calculations_sheet_NH_lab.xlsx">Liposome preparation</a></h3> |
<p>Excel sheet to calculate amount of lipid added when making liposomes. Goes with the protocol Liposomes preparation step-by-step.</p> | <p>Excel sheet to calculate amount of lipid added when making liposomes. Goes with the protocol Liposomes preparation step-by-step.</p> | ||
| − | < | + | <h3><a href="https://www.chemistry.mcmaster.ca/moran-mirabal/resources/SUPPORTED-LIPID-BILAYER-PREPARATION-SOP-9-2015.pdf">Preparation of supported lipid bilayers</a></h3> |
<p>When making supported lipid bilayers it is based on this protocol step 8 to 12.</p> | <p>When making supported lipid bilayers it is based on this protocol step 8 to 12.</p> | ||
| − | < | + | <h3><a href="https://2018.igem.org/Team:UCopenhagen/Protocols_Lipid_Bilayers">Supported lipid bilayers experiments</a></h3> |
<p>Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.</p> | <p>Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.</p> | ||
</html> | </html> | ||
{{UCopenhagen/Footer}} | {{UCopenhagen/Footer}} | ||
Revision as of 15:27, 16 October 2018
Protocols
Internal protocols
Selfmade protocols:Leakiness protocol
Supported Lipid Bilayer protocol
Injection assay protocol
Cloning protocol
Internal protocols
Protocols from other sources:Transformation of bacteria
Protocol from iGEM HQs. We use LB media instead of SOC media.
Competent cells
We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!).
Cloning of constructs*
This protocol describes our strategy when assembling of the constructs that we worked with in lab.
Liposomes preparation step-by-step
Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.
Liposome preparation
Excel sheet to calculate amount of lipid added when making liposomes. Goes with the protocol Liposomes preparation step-by-step.
Preparation of supported lipid bilayers
When making supported lipid bilayers it is based on this protocol step 8 to 12.
Supported lipid bilayers experiments
Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.