Difference between revisions of "Team:UCopenhagen/Protocols"
| Line 5: | Line 5: | ||
<p><i>Selfmade protocols:</i></p> | <p><i>Selfmade protocols:</i></p> | ||
<h3><a href="https://static.igem.org/mediawiki/2018/1/14/T--UCopenhagen--LeakProt.jpeg">Leakiness protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | <h3><a href="https://static.igem.org/mediawiki/2018/1/14/T--UCopenhagen--LeakProt.jpeg">Leakiness protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | ||
| − | <h3><a href="https://static.igem.org/mediawiki/2018/5/57/T--UCopenhagen--SLBProt.jpeg">Supported Lipid Bilayer protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | + | |
| + | <h3><a href="https://static.igem.org/mediawiki/2018/5/57/T--UCopenhagen--SLBProt.jpeg">Supported Lipid Bilayer experiment protocol</a><span style="text-decoration:underline;"></span></h3><p>Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.</p> | ||
| + | |||
<h3><a href="https://static.igem.org/mediawiki/2018/8/82/T--UCopenhagen--onionProt.pdf">Injection assay protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | <h3><a href="https://static.igem.org/mediawiki/2018/8/82/T--UCopenhagen--onionProt.pdf">Injection assay protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | ||
| − | <h3><a href="https://static.igem.org/mediawiki/2018/8/81/T--UCopenhagen--Clone.pdf">Cloning protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | + | |
| − | <h2> | + | <h3><a href="https://static.igem.org/mediawiki/2018/8/81/T--UCopenhagen--Clone.pdf">Cloning of constructs protocol</a><span style="text-decoration:underline;"></span></h3> |
| + | <p> | ||
| + | This protocol describes our strategy when assembling of the constructs that we worked with in lab. | ||
| + | </p> | ||
| + | |||
| + | <h2>External protocols</h2> | ||
| + | |||
<p><i>Protocols from other sources:</i></p> | <p><i>Protocols from other sources:</i></p> | ||
<h3><a href="https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">Transformation of bacteria</a><span style="text-decoration:underline;"></span></h3> | <h3><a href="https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">Transformation of bacteria</a><span style="text-decoration:underline;"></span></h3> | ||
| Line 22: | Line 30: | ||
We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!). | We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!). | ||
</p> | </p> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
<h3><a href="https://static.igem.org/mediawiki/2018/6/6d/T--UCopenhagen--Protocol_Liposome_preparation_step_by_step_NH_lab.pdf">Liposomes preparation step-by-step</a></h3> | <h3><a href="https://static.igem.org/mediawiki/2018/6/6d/T--UCopenhagen--Protocol_Liposome_preparation_step_by_step_NH_lab.pdf">Liposomes preparation step-by-step</a></h3> | ||
<p> Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.</p> | <p> Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.</p> | ||
| Line 37: | Line 40: | ||
<p>When making supported lipid bilayers it is based on this protocol step 8 to 12.</p> | <p>When making supported lipid bilayers it is based on this protocol step 8 to 12.</p> | ||
| − | + | ||
| − | + | ||
</html> | </html> | ||
{{UCopenhagen/Footer}} | {{UCopenhagen/Footer}} | ||
Revision as of 16:07, 16 October 2018
Protocols
Internal protocols
Selfmade protocols:
Leakiness protocol
Supported Lipid Bilayer experiment protocol
Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.
Injection assay protocol
Cloning of constructs protocol
This protocol describes our strategy when assembling of the constructs that we worked with in lab.
External protocols
Protocols from other sources:
Transformation of bacteria
Protocol from iGEM HQs. We use LB media instead of SOC media.
Competent cells
We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!).
Liposomes preparation step-by-step
Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.
Liposome preparation
Excel sheet to calculate amount of lipid added when making liposomes. Goes with the protocol Liposomes preparation step-by-step.
Preparation of supported lipid bilayers
When making supported lipid bilayers it is based on this protocol step 8 to 12.