Difference between revisions of "Team:WPI Worcester"

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{{WPI_Worcester}}
 
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<h1> ICEberg (ISPs Combatting EPSs) </h1>
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<h3>Project Summary</h3>
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<p>With the outbreak of <i>E. coli</i> on romaine lettuce in the news this past year, our team decided to focus on disrupting biofilm formation on crops. Biofilms are protein matrices created by bacteria that protect the bacteria against removal as well as antibiotics. A previous WPI iGEM team ([//2015.igem.org/Team:WPI-Worcester WPI iGEM 2015]) found that antifreeze proteins have anti-biofilm properties. Of particular interest to us are proteins that are derived from plants people already consume, such as those found in carrots, due to being already considered safe for consumption.</p>
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<p>We are intending on genetically modifying crops to produce these antifreeze proteins. For our tests, we will be making a gene gun (inspired by the [//2016.igem.org/Team:Cambridge-JIC/Biolistics Cambridge 2016 iGEM]) to transform samples of lettuce leaf. Then we will determine how this impacts biofilm formation and whether it is a viable solution to the problem of human pathogens on crops.</p>
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      <br><br><h1 style="color: #10394e"><div id="isp_above" class="dissociate">ice structuring proteins</div> ISPs Combatting <div id="eps_below" class="dissociate">extracellular polymeric substances</div>EPSs</h1>
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      <p id="project_snippet">a project on food safety<br>and bacterial biofilms</p>
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<p>Inspired by the 2018 romaine lettuce E. coli outbreak in the United States and the work of the 2015 WPI iGEM team, we investigated methods to prevent biofilms of human pathogens on crops. We analyzed the antibiofilm properties of ice structuring proteins (ISPs), also called antifreeze proteins (AFPs), and curcumin, a component of turmeric. The biofilms were measured by the amount extracellular polymeric substances (EPSs) they produced. In the future, we envision transgenic crops that produce antimicrobial proteins to protect themselves against colonization of human pathogens.
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Revision as of 02:42, 17 October 2018



ice structuring proteins
ISPs Combatting
extracellular polymeric substances
EPSs

a project on food safety
and bacterial biofilms

Inspired by the 2018 romaine lettuce E. coli outbreak in the United States and the work of the 2015 WPI iGEM team, we investigated methods to prevent biofilms of human pathogens on crops. We analyzed the antibiofilm properties of ice structuring proteins (ISPs), also called antifreeze proteins (AFPs), and curcumin, a component of turmeric. The biofilms were measured by the amount extracellular polymeric substances (EPSs) they produced. In the future, we envision transgenic crops that produce antimicrobial proteins to protect themselves against colonization of human pathogens.