Difference between revisions of "Team:CSU CHINA/Notebook"

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<h1>Notebook</h1>
 
<h1>Notebook</h1>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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<p> You know what we did from last winter to autumn! In this Notebook you’ll be able to follow what and when we did it. We documented the experiment protocols and chronological important events, including how we started, how we determined our project, weekly meeting, experiment development, human practice project and many more! Thanks to this Notebook you can travel back in time and see how we managed to get big things done in such a small time frame. Once you get to know the past, you learn to appreciate the present day and the hard work done by CSU_CHINA team. </p>
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<h1>Protocols</h1>
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<h2>Chemical transformation</h2>
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<p>
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Thaw 50µL DH10B competent E. coli cells on ice. <br/>
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Add 5 µl DNA from a ligation reaction mix or 10-100ng DNA of a plasmid. <br/>
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Place the mixture on ice for 20-30 minutes. <br/>
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Heat shock at 42°C for 90 seconds. <br/>
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Place on ice for 5 minutes. <br/>
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Pipette 200 µl of room temperature LB media into the mixture. <br/>
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Incubate at 37°C and 220 rpm for 60 minutes. <br/>
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Add 50-100 µl of the transformed cells to the selection plate. <br/>
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Revision as of 10:16, 17 October 2018


Notebook

You know what we did from last winter to autumn! In this Notebook you’ll be able to follow what and when we did it. We documented the experiment protocols and chronological important events, including how we started, how we determined our project, weekly meeting, experiment development, human practice project and many more! Thanks to this Notebook you can travel back in time and see how we managed to get big things done in such a small time frame. Once you get to know the past, you learn to appreciate the present day and the hard work done by CSU_CHINA team.

Protocols

Chemical transformation

Thaw 50µL DH10B competent E. coli cells on ice.
Add 5 µl DNA from a ligation reaction mix or 10-100ng DNA of a plasmid.
Place the mixture on ice for 20-30 minutes.
Heat shock at 42°C for 90 seconds.
Place on ice for 5 minutes.
Pipette 200 µl of room temperature LB media into the mixture.
Incubate at 37°C and 220 rpm for 60 minutes.
Add 50-100 µl of the transformed cells to the selection plate.

What should this page have?

  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.

Inspiration

You can see what others teams have done to organize their notes: