Difference between revisions of "Team:SZU-China/Demonstrate"

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We designed an experiment to investigate the lethal effects of modified emulsifiable powder and wild-type. We prepared the modified and wild-type emulsifiable powder, and 20 cockroaches were divided into two groups. After anesthesia with ether, 10 μl of emulsifiable powder was applied to the abdomen of the abdomen and allowed to stand at 25 ° C to observe the death of sputum. The result showed that the modified emulsifiable powder was much effective.
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We designed an experiment to investigate the lethal effects of modified emulsifiable powder and wild-type.
 
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<p>
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In wild type M.anisopliae group, we used 109 emulsifiable powder 10μl to infects 17 cockroaches individually. While, in transformant group, we user 109 emulsifiable powder 10μl to infects 10 cockroaches individually.
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The results showed that wild type M.anisopliae toke effect after two days, while transformant took effects less than one day. For mortality each days, transformant showed above two times significantly higher (P
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<0.05) mortality than wild type. Two groups reached stationary state after four days, above 20% mortality in wild type, while 50% in transformant. </p>
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Table 1: Experimental data of cockroaches infection experiment. * Table shows the number of cockroaches alive.
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<img class="card-img-top" src="https://static.igem.org/mediawiki/2018/c/c4/T--SZU-China--Demo-table1.png" />
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<img class="card-img-top" src="https://static.igem.org/mediawiki/2018/8/8a/T--SZU-China--Demo-graph1.png" />
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Figure 1: Mortality of cockroaches between wild type M.anisopliae and transformant.
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We consulted the relevant literature and found that the Metarhizium emulsifiable powder emulsion is easy to fail under the sunlight, so we designed a box that can hold emulsifiable powder, called “Green Ground”. In order to verify the validity of the box(final generation), we designed an experiment, we first applied the emulsifiable powder to two non-woven fabrics, one in the box, we named it “With Box”, the other in the environment which called “Without Box”, and put an equal amount of cockroaches. After three days, the number of dead cockroaches in the two groups was counted. Repeated three times, the average mortality rate of the two groups was calculated and compared, and the mortality rate of the With Box group was higher. (As the chart shows)
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We consulted the relevant literature and found that the Metarhizium emulsifiable powder emulsion is easy to fail under the sunlight, so we designed a box that can hold emulsifiable powder, called “Green Ground”. In order to verify the validity of the box(final generation), we designed an experiment, we first applied the emulsifiable powder to two non-woven fabrics, one in the box, we named it “With Box”, the other in the environment which called “Without Box”, and put an equal amount of cockroaches. After three days, the number of dead cockroaches in the two groups was counted. Repeated three times, the average mortality rate of the two groups was calculated and compared, and the mortality rate of the With Box group was higher. (As the chart shows)
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<th scope="col">With box</th>
 
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To ensure that we can produce spore powder in large quantities, we visited Professor Wang Zhongkang, who told us about the fermentation production and took us to visit the factory, which provided conditions for us to produce products in the future.
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To ensure that we can produce spore powder in large quantities, we visited Professor Wang Zhongkang, who told us about the fermentation production and took us to visit the factory, which provided conditions for us to produce products in the future.
  
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Fermentation production process as shown：
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Fermentation production process as shown：
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We used a series of produced emulsifiable powder in combination with our designed boxes to carry out a series of simulation experiments. According to Professor Wang Zhongkang's suggestion, we chose non-woven fabric as the carrier of the emulsifiable powder, because the emulsifiable powder can be attached to the non-woven fabric in a large amount, which greatly increases the probability of infection.
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<p>
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We used a series of produced emulsifiable powder in combination with our designed boxes to carry out a series of simulation experiments. According to Professor Wang Zhongkang's suggestion, we chose non-woven fabric as the carrier of the emulsifiable powder, because the emulsifiable powder can be attached to the non-woven fabric in a large amount, which greatly increases the probability of infection.
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After producing the emulsion, we applied it to the non-woven cloth and placed the non-woven cloth in area A of the box. Then screw the lid down as shown in the pic through the cylinder to form crevices which are also adjustable. The cockroaches can climb through the slope B and get in contact with Metarhizium anisopliae which will lead to death.</p>
 
After producing the emulsion, we applied it to the non-woven cloth and placed the non-woven cloth in area A of the box. Then screw the lid down as shown in the pic through the cylinder to form crevices which are also adjustable. The cockroaches can climb through the slope B and get in contact with Metarhizium anisopliae which will lead to death.</p>
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We recommend changing a pack of emulsifiable powder every week.
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We recommend changing a pack of emulsifiable powder every week.
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Revision as of 11:52, 17 October 2018

Demonstrate

We designed an experiment to investigate the lethal effects of modified emulsifiable powder and wild-type.

In wild type M.anisopliae group, we used 109 emulsifiable powder 10μl to infects 17 cockroaches individually. While, in transformant group, we user 109 emulsifiable powder 10μl to infects 10 cockroaches individually.

The results showed that wild type M.anisopliae toke effect after two days, while transformant took effects less than one day. For mortality each days, transformant showed above two times significantly higher (P <0.05) mortality than wild type. Two groups reached stationary state after four days, above 20% mortality in wild type, while 50% in transformant.

Table 1: Experimental data of cockroaches infection experiment. * Table shows the number of cockroaches alive.
Figure 1: Mortality of cockroaches between wild type M.anisopliae and transformant.

We consulted the relevant literature and found that the Metarhizium emulsifiable powder emulsion is easy to fail under the sunlight, so we designed a box that can hold emulsifiable powder, called “Green Ground”. In order to verify the validity of the box(final generation), we designed an experiment, we first applied the emulsifiable powder to two non-woven fabrics, one in the box, we named it “With Box”, the other in the environment which called “Without Box”, and put an equal amount of cockroaches. After three days, the number of dead cockroaches in the two groups was counted. Repeated three times, the average mortality rate of the two groups was calculated and compared, and the mortality rate of the With Box group was higher. (As the chart shows)

With box Without box
Group 1 10 4
Group 2 9 2
Group 3 12 5
Mean 9.5 3.0
Variance 2.3 2.3
Mortality 34.44% 12.22%

To ensure that we can produce spore powder in large quantities, we visited Professor Wang Zhongkang, who told us about the fermentation production and took us to visit the factory, which provided conditions for us to produce products in the future.

Fermentation production process as shown：

We used a series of produced emulsifiable powder in combination with our designed boxes to carry out a series of simulation experiments. According to Professor Wang Zhongkang's suggestion, we chose non-woven fabric as the carrier of the emulsifiable powder, because the emulsifiable powder can be attached to the non-woven fabric in a large amount, which greatly increases the probability of infection.

After producing the emulsion, we applied it to the non-woven cloth and placed the non-woven cloth in area A of the box. Then screw the lid down as shown in the pic through the cylinder to form crevices which are also adjustable. The cockroaches can climb through the slope B and get in contact with Metarhizium anisopliae which will lead to death.

We recommend changing a pack of emulsifiable powder every week.