Difference between revisions of "Team:SKLMT-China/Basic Part"
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| + | <h2 class="title">Overview </h2> | ||
| + | <p>Our submitted parts include a promoter library of inner promoters with various strength built in latin<>Pseudomonas fluorescence</latin>-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene. </p> | ||
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<h2 class="title"> Promoter Library</2> | <h2 class="title"> Promoter Library</2> | ||
<p>We are proud to submit a promoter library of inner promoters with various strength built in <i>Pseudomonas fluorescence</i>-pf5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in <i>Pseudomonas fluorescence</i>-pf5. Additionally, this promoter library will support future iGEM teams some help in exploration on <i>Pseudomonas fluorescence</i>-pf5 cells with high capacity in degrading. </p> | <p>We are proud to submit a promoter library of inner promoters with various strength built in <i>Pseudomonas fluorescence</i>-pf5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in <i>Pseudomonas fluorescence</i>-pf5. Additionally, this promoter library will support future iGEM teams some help in exploration on <i>Pseudomonas fluorescence</i>-pf5 cells with high capacity in degrading. </p> | ||
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<img src="https://static.igem.org/mediawiki/2018/e/e3/T--SKLMT-China--promoter_outputy.png | <img src="https://static.igem.org/mediawiki/2018/e/e3/T--SKLMT-China--promoter_outputy.png | ||
" alt="Strength level of different promoters" /> | " alt="Strength level of different promoters" /> | ||
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<h2 class="title"> All Basic Parts</2> | <h2 class="title"> All Basic Parts</2> | ||
Revision as of 13:53, 17 October 2018
Overview
Our submitted parts include a promoter library of inner promoters with various strength built in latin<>Pseudomonas fluorescence-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene.
Promoter Library
We are proud to submit a promoter library of inner promoters with various strength built in Pseudomonas fluorescence-pf5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in Pseudomonas fluorescence-pf5. Additionally, this promoter library will support future iGEM teams some help in exploration on Pseudomonas fluorescence-pf5 cells with high capacity in degrading.
We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.
According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively.
All Basic Parts
| Biobrick | Type | Description | length |
| BBa_K2569001 | Regulatory | Pdiv, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569002 | Regulatory | ParaA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569003 | Regulatory | PfabD, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569005 | Regulatory | PccmD, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569006 | Regulatory | PfliA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569011 | Regulatory | PpltR, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569012 | Regulatory | PproC, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569013 | Regulatory | PrecA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569014 | Regulatory | PrpoD, a constitutive promoter in pseudomonas fluorescence | 70 |
| BBa_K2569015 | Regulatory | PrpoS, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569016 | Regulatory | PdnaA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569017 | Regulatory | Ppol, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569018 | Regulatory | P16s, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569019 | Regulatory | Psig, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569020 | Regulatory | Patf, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569021 | Regulatory | Pfme, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569022 | Regulatory | Pmem, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569023 | Regulatory | Pfat, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569024 | Regulatory | Pami , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569025 | Regulatory | Prib, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569026 | Regulatory | Pabc , constitutive promoter in pseudomonas fluorescence pf-4 | 70 |
| BBa_K2569027 | Regulatory | Pedo , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569028 | Regulatory | Ptox , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569029 | Regulatory | PampC , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569030 | Regulatory | Plip , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569033 | Regulatory | PampR,promoter | 92 |
| BBa_K2569034 | Regulatory | Pdiv, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569036 | Coding | Nicotine oxidase NicA2 | 1449 |