Difference between revisions of "Team:Goettingen/Parts"

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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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    <h2>Parts</h2>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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    <h3>Connecting global research</h3>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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        <p>Our contribution to a huge parts collection.</p>
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    <p>The concept of iGEM is based on the exchange of internationally provided DNA sequences. These sequences are called Biobricks and are collected in a big database, which grows steadily as iGEM progresses. In this way, teams all over the globe can benefit from the part collection and improve the work that was done previously by other teams, to drive the research further.</p>
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    <h3>Basic parts and composite parts</h3>
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    <p>Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table: </p>
 
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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ADD PARTS
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h3>Part Table </h3>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM18 Goettingen</groupparts>
 
<groupparts>iGEM18 Goettingen</groupparts>
 
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<p>Furthermore, we have characterized an existing part. We have selected the part <a href="http://parts.igem.org/Part:BBa_E2050" target="_blank">BBa_E2050 (mERP)</a>, which we have used for transformation of different <em>B. subtilis</em> strains. Because the plasmid pSB1C3 does not contain an origin of replication for <em>B. subtilis</em>, we have cloned the fluorophore gene using the plasmid pAC7 and transformed the <em>E. coli</em> strain DH5α with the resulting plasmids. The fluorophore gene was also fused to a self-made promoter, which is characterized by a good consensus sequence for the housekeeping sigma factor A and a perfect ribosome binding site (RBS) of <em>B. subtilis</em>. Further information can be found on the parts registry sites. Some of the constructed <em>B. subtilis</em> strains were used for the competition experiments (please check out the Results section). </p>
  
  
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Latest revision as of 17:15, 17 October 2018

Parts

Connecting global research

Our contribution to a huge parts collection.

The concept of iGEM is based on the exchange of internationally provided DNA sequences. These sequences are called Biobricks and are collected in a big database, which grows steadily as iGEM progresses. In this way, teams all over the globe can benefit from the part collection and improve the work that was done previously by other teams, to drive the research further.

Basic parts and composite parts

Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table:

<groupparts>iGEM18 Goettingen</groupparts>

Furthermore, we have characterized an existing part. We have selected the part BBa_E2050 (mERP), which we have used for transformation of different B. subtilis strains. Because the plasmid pSB1C3 does not contain an origin of replication for B. subtilis, we have cloned the fluorophore gene using the plasmid pAC7 and transformed the E. coli strain DH5α with the resulting plasmids. The fluorophore gene was also fused to a self-made promoter, which is characterized by a good consensus sequence for the housekeeping sigma factor A and a perfect ribosome binding site (RBS) of B. subtilis. Further information can be found on the parts registry sites. Some of the constructed B. subtilis strains were used for the competition experiments (please check out the Results section).