Difference between revisions of "Team:SKLMT-China/Basic Part"
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<section class="article-banner" style="background-image: url(https://static.igem.org/mediawiki/2018/1/12/T--SKLMT-China--part-banner-2.jpg)"> <div class="banner-content"> | <section class="article-banner" style="background-image: url(https://static.igem.org/mediawiki/2018/1/12/T--SKLMT-China--part-banner-2.jpg)"> <div class="banner-content"> | ||
<h2 class="title">Basic Part</h2> | <h2 class="title">Basic Part</h2> | ||
| − | + | <p class="content">Standard promoter biobrick</p> | |
</div> | </div> | ||
<div class="container"> | <div class="container"> | ||
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</section> | </section> | ||
| − | <div class="container | + | <div class="container"> |
<div class="paragraph shadow" id="s1"> | <div class="paragraph shadow" id="s1"> | ||
<h2 class="title">Overview </h2> | <h2 class="title">Overview </h2> | ||
| − | <p>Our submitted parts include a promoter library of inner promoters with various strength built in | + | <p>Our submitted parts include a promoter library of inner promoters with various strength built in <latin>Pseudomonas fluorescencepf-5</latin>. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene. </p> |
</div> | </div> | ||
<div class="paragraph shadow" id="s2"> | <div class="paragraph shadow" id="s2"> | ||
<h2 class="title">Promoter Library</h2> | <h2 class="title">Promoter Library</h2> | ||
| − | <p>We are proud to submit a promoter library of inner promoters with various strength built in < | + | <p>We are proud to submit a promoter library of inner promoters with various strength built in<latin>P.fluorescencepf-5</latin>. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in <latin>P.fluorescencepf-5</latin>. Additionally, this promoter library will support future iGEM teams some help in exploration on <latin>P.fluorescencepf-5</latin> cells with high capacity in degrading. </p> |
<p>We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.</p> | <p>We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.</p> | ||
<div style="text-align: center"> | <div style="text-align: center"> | ||
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<h2 class="title">All Basic Parts</h2> | <h2 class="title">All Basic Parts</h2> | ||
| − | <table><thead><tr><td>Biobrick</td><td>Type</td><td>Description</td><td>length</td></tr></thead><tbody><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569001">BBa_K2569001</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569002">BBa_K2569002</a></td><td>Regulatory</td><td>ParaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569003">BBa_K2569003</a></td><td>Regulatory</td><td>PfabD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569005">BBa_K2569005</a></td><td>Regulatory</td><td>PccmD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569006">BBa_K2569006</a></td><td>Regulatory</td><td>PfliA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569011">BBa_K2569011</a></td><td>Regulatory</td><td>PpltR, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569012">BBa_K2569012</a></td><td>Regulatory</td><td>PproC, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569013">BBa_K2569013</a></td><td>Regulatory</td><td>PrecA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569014">BBa_K2569014</a></td><td>Regulatory</td><td>PrpoD, a constitutive promoter in pseudomonas fluorescence</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569015">BBa_K2569015</a></td><td>Regulatory</td><td>PrpoS, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569016">BBa_K2569016</a></td><td>Regulatory</td><td>PdnaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569017">BBa_K2569017</a></td><td>Regulatory</td><td>Ppol, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569018">BBa_K2569018</a></td><td>Regulatory</td><td>P16s, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569019">BBa_K2569019</a></td><td>Regulatory</td><td>Psig, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569020">BBa_K2569020</a></td><td>Regulatory</td><td>Patf, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569021">BBa_K2569021</a></td><td>Regulatory</td><td>Pfme, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569022">BBa_K2569022</a></td><td>Regulatory</td><td>Pmem, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569023">BBa_K2569023</a></td><td>Regulatory</td><td>Pfat, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569024">BBa_K2569024</a></td><td>Regulatory</td><td>Pami , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569025">BBa_K2569025</a></td><td>Regulatory</td><td>Prib, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569026">BBa_K2569026</a></td><td>Regulatory</td><td>Pabc , constitutive promoter in pseudomonas fluorescence pf-4</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569027">BBa_K2569027</a></td><td>Regulatory</td><td>Pedo , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569028">BBa_K2569028</a></td><td>Regulatory</td><td>Ptox , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569029">BBa_K2569029</a></td><td>Regulatory</td><td>PampC , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569030">BBa_K2569030</a></td><td>Regulatory</td><td>Plip , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569033">BBa_K2569033</a></td><td>Regulatory</td><td>PampR,promoter</td><td>92</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569034">BBa_K2569034</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569036">BBa_K2569036</a></td><td>Coding</td><td>Nicotine oxidase NicA2</td><td>1449</td></tr></tbody></table><br /><span style="font-family:Arial;"><br /></span><p><span style="font-size: 10.5pt;"><span style="font-family:Arial;"> </span></span></p> | + | <table><thead><tr><td>Biobrick</td><td>Type</td><td>Description</td><td>length</td></tr></thead><tbody><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569001">BBa_K2569001</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><i class="fa fa-star" style="color: blue"></i><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569002">BBa_K2569002</a></td><td>Regulatory</td><td>ParaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569003">BBa_K2569003</a></td><td>Regulatory</td><td>PfabD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569005">BBa_K2569005</a></td><td>Regulatory</td><td>PccmD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569006">BBa_K2569006</a></td><td>Regulatory</td><td>PfliA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569011">BBa_K2569011</a></td><td>Regulatory</td><td>PpltR, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569012">BBa_K2569012</a></td><td>Regulatory</td><td>PproC, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569013">BBa_K2569013</a></td><td>Regulatory</td><td>PrecA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569014">BBa_K2569014</a></td><td>Regulatory</td><td>PrpoD, a constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569015">BBa_K2569015</a></td><td>Regulatory</td><td>PrpoS, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569016">BBa_K2569016</a></td><td>Regulatory</td><td>PdnaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569017">BBa_K2569017</a></td><td>Regulatory</td><td>Ppol, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569018">BBa_K2569018</a></td><td>Regulatory</td><td>P16s, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569019">BBa_K2569019</a></td><td>Regulatory</td><td>Psig, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569020">BBa_K2569020</a></td><td>Regulatory</td><td>Patf, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569021">BBa_K2569021</a></td><td>Regulatory</td><td>Pfme, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569022">BBa_K2569022</a></td><td>Regulatory</td><td>Pmem, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569023">BBa_K2569023</a></td><td>Regulatory</td><td>Pfat, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569024">BBa_K2569024</a></td><td>Regulatory</td><td>Pami , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569025">BBa_K2569025</a></td><td>Regulatory</td><td>Prib, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569026">BBa_K2569026</a></td><td>Regulatory</td><td>Pabc , constitutive promoter in pseudomonas fluorescence pf-4</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569027">BBa_K2569027</a></td><td>Regulatory</td><td>Pedo , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569028">BBa_K2569028</a></td><td>Regulatory</td><td>Ptox , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569029">BBa_K2569029</a></td><td>Regulatory</td><td>PampC , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569030">BBa_K2569030</a></td><td>Regulatory</td><td>Plip , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569033">BBa_K2569033</a></td><td>Regulatory</td><td>PampR,promoter</td><td>92</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569034">BBa_K2569034</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569036">BBa_K2569036</a></td><td>Coding</td><td>Nicotine oxidase NicA2</td><td>1449</td></tr></tbody></table><br /><span style="font-family:Arial;"><br /></span><p><span style="font-size: 10.5pt;"><span style="font-family:Arial;"> </span></span></p> |
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 21:39, 17 October 2018
Overview
Our submitted parts include a promoter library of inner promoters with various strength built in
Promoter Library
We are proud to submit a promoter library of inner promoters with various strength built in
We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.
According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively.
All Basic Parts
| Biobrick | Type | Description | length |
| BBa_K2569001 | Regulatory | Pdiv, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569002 | Regulatory | ParaA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569003 | Regulatory | PfabD, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569005 | Regulatory | PccmD, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569006 | Regulatory | PfliA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569011 | Regulatory | PpltR, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569012 | Regulatory | PproC, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569013 | Regulatory | PrecA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569014 | Regulatory | PrpoD, a constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569015 | Regulatory | PrpoS, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569016 | Regulatory | PdnaA, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569017 | Regulatory | Ppol, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569018 | Regulatory | P16s, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569019 | Regulatory | Psig, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569020 | Regulatory | Patf, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569021 | Regulatory | Pfme, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569022 | Regulatory | Pmem, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569023 | Regulatory | Pfat, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569024 | Regulatory | Pami , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569025 | Regulatory | Prib, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569026 | Regulatory | Pabc , constitutive promoter in pseudomonas fluorescence pf-4 | 70 |
| BBa_K2569027 | Regulatory | Pedo , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569028 | Regulatory | Ptox , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569029 | Regulatory | PampC , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569030 | Regulatory | Plip , constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569033 | Regulatory | PampR,promoter | 92 |
| BBa_K2569034 | Regulatory | Pdiv, constitutive promoter in pseudomonas fluorescence pf-5 | 70 |
| BBa_K2569036 | Coding | Nicotine oxidase NicA2 | 1449 |