Difference between revisions of "Team:SKLMT-China/Basic Part"

(Prototype team page)
 
 
(48 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{SKLMT-China}}
 
 
<html>
 
<html>
  
  
−
<div class="column full_size judges-will-not-evaluate">
+
    <section class="article-banner" style="background-image: url(https://static.igem.org/mediawiki/2018/1/12/T--SKLMT-China--part-banner-2.jpg)"> <div class="banner-content">
−
<h3>★  ALERT! </h3>
+
            <h2 class="title">Basic Part</h2>
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
+
            <p class="content">Standard promoter biobrick</p>
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
        </div>
−
</div>
+
        <div class="container">
  
 +
            <div class="row">
 +
                <ol class="breadcrumb">
 +
                    <li><a href="https://2018.igem.org/Team:SKLMT-China"><i class="fa fa-home">&thinsp;&thinsp;Home</i></a></li>
 +
                    <li><a href="Parts"><i class="fa fa-scissors">&thinsp;&thinsp;Parts</i></a></li>
  
−
<div class="clear"></div>
+
                    <li class="active dropdown">
  
 +
                        <!-- Catalog -->
 +
                        <div class="dropdown-toggle" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
 +
                            <i class="fa fa-code">
 +
                                &thinsp;&thinsp;Basic Part
 +
                            </i>
 +
                        </div>
 +
                        <ul class="dropdown-menu">
 +
                            <li><a href="#s1" class="scrolly">Overview</a></li>
 +
                            <li><a href="#s2" class="scrolly">Promoter Library</a></li>
 +
                            <li><a href="#s3" class="scrolly">All Basic Parts</a></li>
 +
                        </ul>
  
 +
                    </li>
 +
                </ol>
 +
            </div>
 +
        </div>
 +
    </section>
  
−
<div class="column full_size">
+
  <div class="container">
−
<h1>Basic Parts</h1>
+
            <div class="paragraph shadow" id="s1">
−
<p>
+
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
+
−
</p>
+
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
+
<h2 class="title">Overview </h2>
 +
<p>Our submitted parts include a promoter library of inner promoters with various strength built in <latin>Pseudomonas fluorescencepf-5</latin>. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in  enhanced nicotine degradation compared to original nicA2 gene. </p>
 +
  </div>
 +
<div class="paragraph shadow" id="s2">
 +
 
 +
          <h2 class="title">Promoter Library</h2>
 +
            <p>We are proud to submit a promoter library of inner promoters with various strength built in<latin>P.fluorescencepf-5</latin>. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in <latin>P.fluorescencepf-5</latin>. Additionally, this promoter library will support future iGEM teams some help in exploration on <latin>P.fluorescencepf-5</latin> cells with high capacity in degrading. </p>
 +
<p>We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.</p>
 +
<div style="text-align: center">
 +
<img class="lb" src="https://static.igem.org/mediawiki/2018/9/9b/T--SKLMT-China--promoter_y.png" data-src="https://static.igem.org/mediawiki/2018/9/9b/T--SKLMT-China--promoter_y.png" alt="Measurement of promoter strength via measuring firefly fluorescein" />
 
</div>
 
</div>
  
−
 
+
<p>According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively. </p>
−
<div class="column full_size">
+
<div style="text-align: center">
−
<div class="highlight decoration_background">
+
<img class="lb" src="https://static.igem.org/mediawiki/2018/e/e3/T--SKLMT-China--promoter_outputy.png" data-src="https://static.igem.org/mediawiki/2018/e/e3/T--SKLMT-China--promoter_outputy.png" alt="Strength level of different promoters" />
−
<h3>Note</h3>
+
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
+
−
</div>
+
 
</div>
 
</div>
 +
  </div>
 +
<div class="paragraph shadow" id="s3">
  
 +
  <h2 class="title">All Basic Parts</h2>
  
−
<div class="column full_size">
+
<table><thead><tr><td>Biobrick</td><td>Type</td><td>Description</td><td>length</td></tr></thead><tbody><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569001">BBa_K2569001</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><i class="fa fa-star" style="color: blue"></i><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569002">BBa_K2569002</a></td><td>Regulatory</td><td>ParaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569003">BBa_K2569003</a></td><td>Regulatory</td><td>PfabD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569005">BBa_K2569005</a></td><td>Regulatory</td><td>PccmD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569006">BBa_K2569006</a></td><td>Regulatory</td><td>PfliA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569011">BBa_K2569011</a></td><td>Regulatory</td><td>PpltR, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569012">BBa_K2569012</a></td><td>Regulatory</td><td>PproC, constitutive  promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569013">BBa_K2569013</a></td><td>Regulatory</td><td>PrecA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569014">BBa_K2569014</a></td><td>Regulatory</td><td>PrpoD, a constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569015">BBa_K2569015</a></td><td>Regulatory</td><td>PrpoS, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569016">BBa_K2569016</a></td><td>Regulatory</td><td>PdnaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569017">BBa_K2569017</a></td><td>Regulatory</td><td>Ppol, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569018">BBa_K2569018</a></td><td>Regulatory</td><td>P16s, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569019">BBa_K2569019</a></td><td>Regulatory</td><td>Psig, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569020">BBa_K2569020</a></td><td>Regulatory</td><td>Patf, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569021">BBa_K2569021</a></td><td>Regulatory</td><td>Pfme, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569022">BBa_K2569022</a></td><td>Regulatory</td><td>Pmem, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569023">BBa_K2569023</a></td><td>Regulatory</td><td>Pfat, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569024">BBa_K2569024</a></td><td>Regulatory</td><td>Pami , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569025">BBa_K2569025</a></td><td>Regulatory</td><td>Prib, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569026">BBa_K2569026</a></td><td>Regulatory</td><td>Pabc , constitutive promoter in pseudomonas fluorescence pf-4</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569027">BBa_K2569027</a></td><td>Regulatory</td><td>Pedo , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569028">BBa_K2569028</a></td><td>Regulatory</td><td>Ptox , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569029">BBa_K2569029</a></td><td>Regulatory</td><td>PampC , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569030">BBa_K2569030</a></td><td>Regulatory</td><td>Plip , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569033">BBa_K2569033</a></td><td>Regulatory</td><td>PampR,promoter</td><td>92</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569034">BBa_K2569034</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569036">BBa_K2569036</a></td><td>Coding</td><td>Nicotine oxidase  NicA2</td><td>1449</td></tr></tbody></table><br /><span style="font-family:Arial;"><br /></span><p><span style="font-size: 10.5pt;"><span style="font-family:Arial;">&nbsp;</span></span></p>
−
<h3>Best Basic Part Special Prize</h3>
+
</div>
−
 
+
−
<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
+
−
 
+
−
<br><br>
+
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
 
</div>
 
</div>
−
 
−
 
−
 
−
 
  
 
</html>
 
</html>
 +
{{SKLMT-China/footer}}

Latest revision as of 21:39, 17 October 2018

  1.   Home
  2.   Parts

Overview

Our submitted parts include a promoter library of inner promoters with various strength built in Pseudomonas fluorescencepf-5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene.

Promoter Library

We are proud to submit a promoter library of inner promoters with various strength built inP.fluorescencepf-5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in P.fluorescencepf-5. Additionally, this promoter library will support future iGEM teams some help in exploration on P.fluorescencepf-5 cells with high capacity in degrading.

We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.

Measurement of promoter strength via measuring firefly fluorescein

According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively.

Strength level of different promoters

All Basic Parts

BiobrickTypeDescriptionlength
BBa_K2569001RegulatoryPdiv, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569002RegulatoryParaA, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569003RegulatoryPfabD, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569005RegulatoryPccmD, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569006RegulatoryPfliA, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569011RegulatoryPpltR, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569012RegulatoryPproC, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569013RegulatoryPrecA, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569014RegulatoryPrpoD, a constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569015RegulatoryPrpoS, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569016RegulatoryPdnaA, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569017RegulatoryPpol, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569018RegulatoryP16s, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569019RegulatoryPsig, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569020RegulatoryPatf, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569021RegulatoryPfme, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569022RegulatoryPmem, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569023RegulatoryPfat, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569024RegulatoryPami , constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569025RegulatoryPrib, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569026RegulatoryPabc , constitutive promoter in pseudomonas fluorescence pf-470
BBa_K2569027RegulatoryPedo , constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569028RegulatoryPtox , constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569029RegulatoryPampC , constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569030RegulatoryPlip , constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569033RegulatoryPampR,promoter92
BBa_K2569034RegulatoryPdiv, constitutive promoter in pseudomonas fluorescence pf-570
BBa_K2569036CodingNicotine oxidase NicA21449


 

SKLMT

Useful  Links

Main Page

Special pages

Contact  us


  SKLMT.iGEM.China@gmail.com

Address


  Qingdao, Shandong


266200 Shandong University