Difference between revisions of "Team:SZU-China/Description"

(Prototype team page)
 
 
(21 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{SZU-China}}
+
{{SZU-China/header}}
 
<html>
 
<html>
  
 +
<style>
 +
h1,
 +
h2 {
 +
color: #469789;
 +
}
 +
 +
.nav-pills .nav-link.active,
 +
.nav-pills .show>.nav-link {
 +
color: #fff !important;
 +
background-color: #469789;
 +
}
 +
 +
#myScrollspy>nav>a {
 +
color: #469789;
 +
}
 +
.card{
 +
border-top: #469789;
 +
}
 +
.border-3 {
 +
    border-width:3px !important;
 +
    border-left: none !important;
 +
    border-right: none !important;
 +
    border-bottom: none !important;
 +
   
 +
}
 +
.card[class*="border"] {
 +
    border: 1px solid #469789;
 +
    -webkit-box-shadow: none;
 +
    box-shadow: none;
 +
}
 +
</style>
  
 +
<body data-spy="scroll" data-target="#myScrollspy" data-offset="10">
 +
<div class="container-full">
 +
<div class="card">
 +
<img class="card-img-top" src="https://static.igem.org/mediawiki/2018/c/c4/T--SZU-China--descri.jpg" />
 +
</div>
 +
</div>
 +
<div class="container-fluid">
 +
<div class="row">
 +
<div class="col-9 offset-1 shadow ">
 +
<div class="text-center">
 +
<h1>Description</h1>
 +
</div>
 +
<div id="Background" class="indent">
 +
<h2>Background</h2>
 +
<p>
 +
Cockroaches are among the most common and obnoxious household pests, they harbor in damp and unsanitary places such as sewers, garbage disposals, kitchens, and bathroom, feed on human’s and pet’s food. Besides its importance as a sign of poor sanitation, cockroaches have been implicated in the transmission of several pathogenic organisms such as <i>E.coli</i> and <i>Salmonella enteritidis</i> which can cause diarrhea, pneumonia and so on<sup>[1]</sup>. Also, expose to cockroach feces and body parts of dead roaches over time can trigger allergies and asthma<sup>[2]</sup>. So it’s not safe to ignore these pests.
  
−
<div class="column full_size">
+
</p>
−
<h1>Description</h1>
+
</div>
 +
<div id="Current" class="indent">
 +
<h2>Current solution</h2>
 +
<p>
 +
People rack their brains about cockroach control. Currently, there are two mainstream ways to control the population of cockroaches, physical and chemical methods. However, a chemical method like foggers, boric acid, and gel bait may cause environmental toxicity and the development of resistance in cockroaches<sup>[3]</sup>. A physical method such as “cockroach house”, only trap few cockroaches. Neither chemical nor physical method can’t achieve the goal of environment-friendly and efficient control.
  
−
<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
+
</p>
  
−
</div>
+
</div>
 +
<div id="solution" class="indent">
 +
<h2>Our solution</h2>
 +
<p>
 +
This year’s SZU China team want to tackle the cockroach control issue by using synthetic biology. We choose a kind of entomogenous fungi, <i>Metarhizium anisopliae </i>, as biological chassis. It is considered a safe and prospective choice for causing disease in insects only. But its lethality is still needed to be improved, which limits it's widespread used. We construct a system to enhance its virulence. Our system contains three parts: HsbA, BbChit, and MCL1. These three genes work sequentially during infecting cockroach, promote adhesion, penetration, and immune-avoidance respectively.
  
 +
</p>
  
 +
</div>
 +
<div class="row">
 +
<div class="col-lg-4 col-md-10">
 +
<div class="card text-center border-3 shadow h-100">
 +
<div class="view text-center">
 +
<img class="card-img-top" style="width: 80px;" src="https://static.igem.org/mediawiki/2018/2/2a/T--SZU-China--Descrip1.png" />
  
−
<div class="column two_thirds_size">
+
</div>
−
<h3>What should this page contain?</h3>
+
<div class="card-body text-center">
−
<ul>
+
<h3 style=" color: #469789;">HsbA</h3>
−
<li> A clear and concise description of your project.</li>
+
<p>
−
<li>A detailed explanation of why your team chose to work on this particular project.</li>
+
It encodes hydrophobic surface binding protein A, located on the surface of fungus. This protein works like “glue” by forming a hydrophobic bond between spores and the waxy epicuticle of their host.
−
<li>References and sources to document your research.</li>
+
</p>
−
<li>Use illustrations and other visual resources to explain your project.</li>
+
</div>
−
</ul>
+
</div>
−
</div>
+
</div>
 +
<div class="col-lg-4 col-md-10">
 +
<div class="card border-3 shadow h-100">
 +
<div class="view text-center ">
 +
<img class="card-img-top" style="width: 128px;" src="https://static.igem.org/mediawiki/2018/8/85/T--SZU-China--Descrip2.png" />
  
−
<div class="column third_size" >
+
</div>
−
<div class="highlight decoration_A_full">
+
<div class="card-body text-center">
−
<h3>Inspiration</h3>
+
−
<p>See how other teams have described and presented their projects: </p>
+
<h3 style=" color: #469789;">Bbchit</h3>
 +
 +
<p>
 +
It encodes chitinase, which can hydrolyze chitin. Chitosan is the major part of the insect body wall, chitinase can hydrolyze this to penetrate.
 +
</p>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="col-lg-4 col-md-10">
 +
<div class="card border-3 shadow h-100">
 +
<div class="view text-center">
 +
<img class="card-img-top" style="width: 120px;" src="https://static.igem.org/mediawiki/2018/c/cc/T--SZU-China--Descrip3.png" />
  
−
<ul>
+
</div>
−
<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
+
<div class="card-body text-center">
−
<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
+
<h3 style=" color: #469789;">MCL1</h3>
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
+
<p>
−
<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
+
It encodes a collagen-like protein, which can combine with β-glucan.β-glucan is distributed on the cell wall surface of fungus and is the recognization site for insect hemocytes to combine and clear invader. MCL1 acts like putting an “invisible cloak” on the fungus. so that fungus can evade being recognized by the host immune system.
−
</ul>
+
−
</div>
+
−
</div>
+
  
 +
</p>
  
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
  
  
−
<div class="column two_thirds_size" >
+
<div class="indent">
−
<h3>Advice on writing your Project Description</h3>
+
  
−
<p>
+
<p>
−
We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.  
+
Then, we design a trap box named GreenGround to bring our idea into real world. We mix spores,banana powder and oil together to form emulsifiable powder,which is applied to non-woven fabrics for use.
−
</p>
+
(<a style=" color: #469789;" href="https://2018.igem.org/Team:SZU-China/Applied_Design">Click, and check more details about our box</a>)
  
−
</div>
+
</p>
 +
</div>
 +
<div id="Suicide" class="indent">
 +
<h2>Suicide switch</h2>
 +
<p>
 +
We built a safety mechanism for our system. It consists of a tryptophan attenuator and a MazF venom protein. In the cultivating and cockroaches environment, the Tryptophan attenuator makes a difference that stops the translation before MazF, and then the fungi can keep alive. If the fungi abscise the environment we mention above, the Tryptophan attenuator would not work and the MazF expresses to make them die quickly.<sup>[4]</sup>
 +
(<a style=" color: #469789;" href="https://2018.igem.org/Team:SZU-China/Design">Click and check ‘How to ensure safety’ for more details</a>)
  
−
<div class="column third_size">
+
</p>
−
<h3>References</h3>
+
</div>
−
<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
+
<div id="References" class="indent">
 +
<h2>References</h2>
 +
<p>
 +
[1] Mpuchane S, Matsheka IM, Gashe BA, Allotey J, Murindamombe G, Mrema N. Microbiological studies of cockroaches from three localities in Gaborone, Botswana. Afr J Food Nutr Sci. 2006;6:56–59.
 +
</p>
 +
<p>
 +
[2] Gore JC, Schal C,Cockroach allergen biology and mitigation in the indoor environment. Annu Rev Entomol. 2007; 52():439-63.
  
−
</div>
+
</p>
 +
<p>
 +
[3] Wu X, Appel AG.J Econ Entomol. 2017 Jun 1;110(3):1203-1209.
 +
</p>
 +
<p>
 +
[4] Sowa S M, Keeley L L. Free amino acids in the hemolymph of the cockroach, Blaberus discoidalis[J]. Comparative Biochemistry & Physiology Part A Physiology, 1996, 113(2):131.
 +
</p>
 +
</div>
  
 +
</div>
  
 +
<div class="col-2">
  
 +
<nav id="myScrollspy" class="navbar position-fixed">
 +
<nav class="nav nav-pills flex-column">
 +
<a class="nav-link" href="#Background">Background</a>
 +
<a class="nav-link" href="#Current">Current solution</a>
 +
<a class="nav-link" href="#solution">Our solution</a>
 +
<a class="nav-link" href="#Suicide">Suicide switch</a>
 +
<a class="nav-link" href="#References">References</a>
 +
 +
</nav>
 +
</nav>
 +
 +
</div>
 +
 +
</div>
 +
 +
</div>
 +
<script>
 +
$('body').scrollspy({
 +
target: '#myScrollspy',
 +
offset: 10
 +
});
 +
 +
var scroll = new SmoothScroll('a[href*="#"]');
 +
</script>
 +
</body>
  
  
  
 
</html>
 
</html>

Latest revision as of 22:09, 17 October 2018

Description

Background

Cockroaches are among the most common and obnoxious household pests, they harbor in damp and unsanitary places such as sewers, garbage disposals, kitchens, and bathroom, feed on human’s and pet’s food. Besides its importance as a sign of poor sanitation, cockroaches have been implicated in the transmission of several pathogenic organisms such as E.coli and Salmonella enteritidis which can cause diarrhea, pneumonia and so on[1]. Also, expose to cockroach feces and body parts of dead roaches over time can trigger allergies and asthma[2]. So it’s not safe to ignore these pests.

Current solution

People rack their brains about cockroach control. Currently, there are two mainstream ways to control the population of cockroaches, physical and chemical methods. However, a chemical method like foggers, boric acid, and gel bait may cause environmental toxicity and the development of resistance in cockroaches[3]. A physical method such as “cockroach house”, only trap few cockroaches. Neither chemical nor physical method can’t achieve the goal of environment-friendly and efficient control.

Our solution

This year’s SZU China team want to tackle the cockroach control issue by using synthetic biology. We choose a kind of entomogenous fungi, Metarhizium anisopliae , as biological chassis. It is considered a safe and prospective choice for causing disease in insects only. But its lethality is still needed to be improved, which limits it's widespread used. We construct a system to enhance its virulence. Our system contains three parts: HsbA, BbChit, and MCL1. These three genes work sequentially during infecting cockroach, promote adhesion, penetration, and immune-avoidance respectively.

HsbA

It encodes hydrophobic surface binding protein A, located on the surface of fungus. This protein works like “glue” by forming a hydrophobic bond between spores and the waxy epicuticle of their host.

Bbchit

It encodes chitinase, which can hydrolyze chitin. Chitosan is the major part of the insect body wall, chitinase can hydrolyze this to penetrate.

MCL1

It encodes a collagen-like protein, which can combine with β-glucan.β-glucan is distributed on the cell wall surface of fungus and is the recognization site for insect hemocytes to combine and clear invader. MCL1 acts like putting an “invisible cloak” on the fungus. so that fungus can evade being recognized by the host immune system.

Then, we design a trap box named GreenGround to bring our idea into real world. We mix spores,banana powder and oil together to form emulsifiable powder,which is applied to non-woven fabrics for use. (Click, and check more details about our box)

Suicide switch

We built a safety mechanism for our system. It consists of a tryptophan attenuator and a MazF venom protein. In the cultivating and cockroaches environment, the Tryptophan attenuator makes a difference that stops the translation before MazF, and then the fungi can keep alive. If the fungi abscise the environment we mention above, the Tryptophan attenuator would not work and the MazF expresses to make them die quickly.[4] (Click and check ‘How to ensure safety’ for more details)

References

[1] Mpuchane S, Matsheka IM, Gashe BA, Allotey J, Murindamombe G, Mrema N. Microbiological studies of cockroaches from three localities in Gaborone, Botswana. Afr J Food Nutr Sci. 2006;6:56–59.

[2] Gore JC, Schal C,Cockroach allergen biology and mitigation in the indoor environment. Annu Rev Entomol. 2007; 52():439-63.

[3] Wu X, Appel AG.J Econ Entomol. 2017 Jun 1;110(3):1203-1209.

[4] Sowa S M, Keeley L L. Free amino acids in the hemolymph of the cockroach, Blaberus discoidalis[J]. Comparative Biochemistry & Physiology Part A Physiology, 1996, 113(2):131.