Difference between revisions of "Team:Bilkent-UNAMBG/InterLab"

 
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     <div class="hipsum"><p>Intelligentsia lo-fi pug Austin. Shabby chic asymmetrical jean shorts, twee stumptown bespoke art party keffiyeh High Life PBR 90's mumblecore. Meggings Echo Park narwhal you probably haven't heard of them, viral locavore Pinterest keytar.  Master cleanse Neutra Marfa cornhole roof party, seitan ennui Kickstarter fingerstache. Ethical tofu retro, paleo pork belly hashtag deep v flexitarian meh High Life Godard occupy lo-fi.  Hella pour-over Williamsburg post-ironic narwhal Kickstarter, tofu single-origin coffee vinyl irony cray jean shorts.  Cray banjo Thundercats, asymmetrical pickled paleo butcher cliche disrupt Odd Future photo booth.</p><p>Forage selfies Shoreditch farm-to-table swag. YOLO Vice organic, Brooklyn meggings banh mi art party. Occupy flexitarian freegan mustache shabby chic hoodie normcore.  Vinyl chia gastropub, asymmetrical fingerstache sartorial ennui aesthetic hella farm-to-table Blue Bottle.  Raw denim cliche semiotics, letterpress bicycle rights cornhole art party YOLO yr Shoreditch leggings brunch fap crucifix.  Gentrify mustache leggings ethnic roof party, shabby chic viral Wes Anderson 3 wolf moon Tonx ugh sustainable photo booth master cleanse meggings.  Shoreditch before they sold out direct trade  hoodie, leggings Portland artisan art party.</p><p>VHS jean shorts Neutra gastropub cornhole, mumblecore Wes Anderson typewriter Godard bitters 3 wolf moon Odd Future tofu tousled plaid. Tonx Etsy tofu, distillery retro blog Blue Bottle ugh kale chips flexitarian Odd Future sartorial Banksy bitters.  Messenger bag XOXO sustainable viral semiotics Williamsburg, whatever gastropub hashtag master cleanse.  Fap Williamsburg Blue Bottle, yr flannel biodiesel XOXO YOLO freegan pour-over farm-to-table leggings.  Ethical Thundercats umami gluten-free fingerstache.  DIY Etsy disrupt PBR.  Shabby chic sriracha synth Pinterest Marfa aesthetic dreamcatcher salvia, trust fund cliche Brooklyn.</p><p>Master cleanse Neutra Marfa cornhole roof party, seitan ennui Kickstarter fingerstache.  Ethical tofu retro, paleo.</p>
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     <div class="hipsum"><p style="text-align: center;">&nbsp;</p>
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<p style="line-height: 1.3800000000000001; margin-top: 0pt; margin-bottom: 10pt;"><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">iGEMs InterLab study aims to establish a standard for developing a robust measurement protocol for GFP. GFP is chosen because it is one of the most used biological markers in synthetic biology laboratories. </span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">Fluorescence data has been a poorly comparable measure since data is reported in various units or different groups process this data in different ways. That is one of the main reasons that fluorescence data is hard to share and compare between labs, which makes it harder to collaborate and troubleshoot.</span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">Over the years, iGEM community has advanced in establishing a baseline for this protocol.</span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">For the Fifth InterLab study, it is aimed to eliminate one more variable measure which is OD because OD measures yield high variability between labs and it is unclear how good an OD can approximate the number of cells. </span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;"><br /></span><span style="font-size: 12pt; font-family: Calibri; color: #000000; background-color: transparent; font-weight: 400; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; text-decoration: none; vertical-align: baseline; white-space: pre-wrap;">Therefore, the Fifth InterLab study is asking if the lab to lab variability in fluorescence measurements can be reduced by normalizing to absolute cell count or CFUs instead of OD? </span></p>
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Latest revision as of 23:13, 17 October 2018

Introduction

 

iGEMs InterLab study aims to establish a standard for developing a robust measurement protocol for GFP. GFP is chosen because it is one of the most used biological markers in synthetic biology laboratories.

Fluorescence data has been a poorly comparable measure since data is reported in various units or different groups process this data in different ways. That is one of the main reasons that fluorescence data is hard to share and compare between labs, which makes it harder to collaborate and troubleshoot.

Over the years, iGEM community has advanced in establishing a baseline for this protocol.
For the Fifth InterLab study, it is aimed to eliminate one more variable measure which is OD because OD measures yield high variability between labs and it is unclear how good an OD can approximate the number of cells.

Therefore, the Fifth InterLab study is asking if the lab to lab variability in fluorescence measurements can be reduced by normalizing to absolute cell count or CFUs instead of OD?