Difference between revisions of "Team:UCopenhagen/Protocols"
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<h1>Protocols</h1> | <h1>Protocols</h1> | ||
<h2>Internal protocols</h2> | <h2>Internal protocols</h2> | ||
| − | <p><i> | + | <p><i>Homemade protocols:</i></p> |
| − | <h3><a href="https://static.igem.org/mediawiki/2018/ | + | <h3><a href="https://static.igem.org/mediawiki/2018/c/c3/T--UCopenhagen--LEAKPROT.pdf">Leakiness protocol</a><span style="text-decoration:underline;"></span></h3><p></p> |
| − | <h3><a href="https://static.igem.org/mediawiki/2018/ | + | <h3><a href="https://static.igem.org/mediawiki/2018/1/1d/T--UCopenhagen--SLBPROT.pdf">Supported Lipid Bilayer experiment protocol</a><span style="text-decoration:underline;"></span></h3><p>Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.</p> |
| − | <h3><a href="https://static.igem.org/mediawiki/2018/9/99/T--UCopenhagen--Clone%281%29.pdf">Injection assay protocol</a><span style="text-decoration:underline;"></span></h3><p></p> | + | <h3><a href="https://static.igem.org/mediawiki/2018/9/99/T--UCopenhagen--Clone%281%29.pdf">Injection assay protocol</a><span style="text-decoration:underline;"></span></h3><p>Protocol for experiments with protoplasts. We have based our protocol losely on the <a href="https://2011.igem.org/Team:HokkaidoU_Japan/Project/Onion">Hokkaido 2011 iGEM</a> teams protocol. We used the same buffers but prepared the protoplasts differently.</p> |
<h3><a href="https://static.igem.org/mediawiki/2018/c/cb/T--UCopenhagen--Clone_Protocol%281%29.pdf">Cloning of constructs protocol</a><span style="text-decoration:underline;"></span></h3> | <h3><a href="https://static.igem.org/mediawiki/2018/c/cb/T--UCopenhagen--Clone_Protocol%281%29.pdf">Cloning of constructs protocol</a><span style="text-decoration:underline;"></span></h3> | ||
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</p> | </p> | ||
| + | <h3><a href="https://static.igem.org/mediawiki/2018/c/cd/T--UCopenhagen--WestAndSDS.pdf">Western blot and SDS-PAGE protocol</span></a></h3> | ||
| + | <p> | ||
| + | This protocol was provided to us from our supervisors in lab. | ||
| + | </p> | ||
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| + | <h3><a href="https://l.facebook.com/l.php?u=http%3A%2F%2F2018.igem.org%2Fwiki%2Fimages%2Ff%2Ff2%2FT--UCopenhagen--Protocol_Injection_into_Liposome.pdf&h=AT1WlnERDElcvph-vAG86KgQAcdcVaXibpSW9YZsVtLQhrg-SD3n6CJ-und74cYh5frFO_FQnT0yjG5yFzeQePvXa4Fp8yu_JqY1G0ELk8a2_gz_DnJXbEJ117mZgOB0G9UwCQ">Injection into liposome protocol</span></a></h3> | ||
| + | <p>Protocol overview describing the different experiments we performed with SIEC secretion into liposomes.</p> | ||
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| + | |||
| + | <p></p> | ||
<h2>External protocols</h2> | <h2>External protocols</h2> | ||
Latest revision as of 00:49, 18 October 2018
Protocols
Internal protocols
Homemade protocols:
Leakiness protocol
Supported Lipid Bilayer experiment protocol
Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.
Injection assay protocol
Protocol for experiments with protoplasts. We have based our protocol losely on the Hokkaido 2011 iGEM teams protocol. We used the same buffers but prepared the protoplasts differently.
Cloning of constructs protocol
This protocol describes our strategy when assembling of the constructs that we worked with in lab.
Western blot and SDS-PAGE protocol
This protocol was provided to us from our supervisors in lab.
Injection into liposome protocol
Protocol overview describing the different experiments we performed with SIEC secretion into liposomes.
External protocols
Protocols from other sources:
Transformation of bacteria
Protocol from iGEM HQs. We use LB media instead of SOC media.
Competent cells
We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!).
Liposomes preparation step-by-step
Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.
Liposome preparation
Excel sheet to calculate amount of lipid added when making liposomes. Goes with the protocol Liposomes preparation step-by-step.
Preparation of supported lipid bilayers
When making supported lipid bilayers it is based on this protocol step 8 to 12.