Difference between revisions of "Team:Nanjing-China/Team"

 
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<body>
 
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Team</a></li></ul></div>
 +
            <ul>
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    <li><a href="#IA">introduction</a></li>
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    <li><a href="#photo">team_photos</a></li></ul>
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<div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></ul></li></div>
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      <div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li></ul></div>
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      <div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges">For_Judges</a></li></ul></div>
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</div>
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</div>
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<div id="for_judge" align="center"><div class="i"><ul><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges"><strong>For_judges</strong></a></ul></div></div>
 
<div class="container" align="center">
 
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    <ul>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li>
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</ul>
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    </li>
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    <li><a href="https://2017.igem.org/Team:Nanjing-China/Parts">PARTS</a>
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         <ul>
 
         <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
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  <li><a href="https://2018.igem.org/Team:Nanjing-China">N<font size="-1"><sub>2</sub></font> CHASER</a>
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            </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
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     <ul>
 
     <ul>
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             <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Team</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">Sliver</a></li>
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             <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
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             <li><a href="https://2018.igem.org/Team:Nanjing-China/Gold">Gold</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
 +
             <li><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges">For_Judges</a></li>
 
</ul>
 
</ul>
−
      </li>
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    </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">PROJECT</a>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">PROJECT</a>
 
     <ul>
 
     <ul>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
 
         <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
 
         <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
−
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li>
 
 
                 <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
 
                 <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
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                 <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate"><font size="-0.1">Demonstrate</font></a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improvement">Improvement</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Hardware">Hardware</a></li>  
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li>
 
</ul>
 
</ul>
 
         </li>
 
         </li>
−
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">TEAM</a>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a>
−
    <ul>
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        <ul>
−
         <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Introduction</a></li>
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         <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
−
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
+
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li>
−
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improve">Improve</a></li>
−
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
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</ul>
 
</ul>
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     </li>
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            </li>
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     <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">OTHERS</a>
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     <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">MODELING</a></a>
 +
      </li>
 +
     <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">PRACTICES</a>
 
     <ul>
 
     <ul>
−
         <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li>
+
         <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices"><font size="-1">Human_Practices</font></a></li>
 
                 <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
 
                 <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
−
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></li>
+
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations"><font size="-0.1">Collaboration</font></a></li>
 
</ul>
 
</ul>
 
     </li>
 
     </li>
 +
    <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">NOTEBOOK</a></li>
 
       </ul>
 
       </ul>
 
   </div>
 
   </div>
−
   </div>   
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   <div class="header"><img src="https://static.igem.org/mediawiki/2018/a/a7/T--Nanjing-China--title-5.png" width="100%"onload="MM_effectAppearFade(this, 1000, 0, 100, false);MM_effectBlind('HOME', 1000, '0%', '100%', true)" >
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    <div class="contain">
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</div>
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    <div id="difinition">
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  <div class="contain">
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    <div class="word">
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    <div class="word" id="IA" align="center">
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    <h2>The definition of nitrogen fixation</h2>
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    <div style="position:absolute; top:-60px; z-index:3;">
−
    <div class="word-2">
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    <img src="https://static.igem.org/mediawiki/2018/d/d7/T--Nanjing-China--team-t.png" width="50%" /></div>
−
    <h3>•What is nitrogen fixation?</h3>
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    <div class="word-1" style="height:60px;"></div>
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<blockquote>
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    <h2>Abstract</h2>
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  <p align="left"><font color="#FF9900"> Nitrogen → ammonia&nbsp;(NH<sub>3</sub>)</font>  or other molecules available to living organisms. </p>
+
    <p>Hello, everyone! We are a group of students  with everlasting passion and perseverance from Nanjing University, and we can&rsquo;t  wait to share with you those stories of our incredible iGEM team!</p>
−
  </blockquote>
+
     <p>We have been on our journey of iGEM as  sophomores and seniors since 2017. Though, at the very beginning, most of us  were not so familiar with synthetic biology and relevant experimental skills in  molecular biology, we still decided to embrace this challenging yet truly rewarding competition, and it turns out that this is the wisest decision we have ever  made, for not only does the competition grant us with valuable experiences of scientific researches, but also helps us establish friendship with team members, a sense of cooperation, and the enthusiasm for science, which shall never fade from our memories.</p>
−
        <div><img src="https://static.igem.org/mediawiki/2018/1/17/T--Nanjing-China--i-background-1.png" width="80%" /></div>
+
     <p>Our team consists of students from Life  Science Department, all of whom are nice colleagues to work with and  interesting souls to communicate with. We inspire each other a lot in every aspect during the whole process—from seeking for innovative ideas, operating complicated experiments, carrying out human  practices, designing wiki and posters to present our work completely at  last. Tremendous support is also consistently and nicely offered by our enthusiastic advisers, instructors and sponsors. Genuinely, we thank you all, for we couldn&rsquo;t  have enjoyed doing this without the effort made by every one of you!</p>
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<div class="word-2">
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    <div><img src="https://static.igem.org/mediawiki/2018/2/2a/T--Nanjing-China--i-background-2.png" width="50%" /></div>
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−
    <h2>What is nitrogen fixation for?</h2>
+
−
        <p align="left"> <font color="#339900">-global food supply <br/>  
+
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-reduce the use of chemical nitrogen fertilizers </font> </p>  
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</div>
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     <div class="word"><div class="word-background-block" style="width:100%;">    </div>
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     <h4>Nitrogen fixation is essential for life. </h4>
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     </div>
 +
<div class="word" id="photo" align="center">
 +
    <div id="click"><font color="#CC3333"><p><h4><font size="120%">☟</font>(Check below to see our teammates, advisors and instructors!)</h4></p></font></div>
 +
        <a href="https://2018.igem.org/Team:Nanjing-China/Members"><img src="https://static.igem.org/mediawiki/2018/5/57/T--Nanjing-China--all1.jpg" name="all1" width="80%" id="all1" onmouseover="MM_effectShake('click')" /></a>
 
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  <h2>The methods of nitrogem fixation</h2>
 
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    <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-3.gif" width="80%" /></div>
 
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    <h3>Nitrogen cycle</h3>
 
−
    <p align="left"><font color="#FF6600">•Nitrogen fixation:</font><br/>
 
−
          —N<sub>2 </sub>→ plants by bacteria<br/>
 
−
    <font color="#FF6600">•Nitrification:</font><br/>
 
−
      —ammonium → nitrite  → nitrate<br/>
 
−
      —Absorbed by plants<br/>
 
−
    <font color="#FF6600">•Denitrification:</font><br/>
 
−
      —Release N to atmosphere</p>
 
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<div class="word-background-block" style="height:180px"></div>
 
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    <h3>Haber-Bosch process </h3>
 
−
    <p align="left"><font color="#FF6600">N2 + 3H<sub>2</sub> → 2NH<sub>3</sub></font>(ΔH° = −91.8 kJ)<br/>
 
−
    <font color="#FF6600">High temperature<br/>
 
−
High pressure<br />
 
−
Need too much energy<br /></font><br/>
 
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                        </p>
 
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    <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-4.gif" width="80%" /></div>
 
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  <div class="word-background-block"></div>
 
−
  <h3>Biological nitrogen fixation</h3>
 
−
    <div class="word-2" style="width:60%"><img src="https://static.igem.org/mediawiki/2018/2/20/T--Nanjing-China--i-background-5.png" width="90%" /></div>
 
−
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−
        <p><font color="#CC66FF">Mild reaction conditions</font></p>
 
−
        <p><font color="#0033CC">Relatively inexpensive</font></p>
 
−
        <p><font color="#996600">High efficiency </font></p>
 
−
        <p><font color="#0099FF">Environmentally friendly</font></p>
 
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        <div class="word-background-block"></div>
 
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        </div>
 
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  <div id="choose">
 
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  <div class="word-background-block"></div>
 
−
  <h2>The choose of nitrogenase</h2>
 
−
  <div class="word">
 
−
  <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/f/f5/T--Nanjing-China--i-background-6.png" width="90%" />
 
−
  <p>Molybdenum <br />(MoFe)-dependent</p></div>
 
−
  <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/a/a9/T--Nanjing-China--i-background-7.png" width="90%" />
 
−
  <p>Vanadium <br />(VFe)-dependent</p></div>
 
−
  <div class="word-3" ><img src="https://static.igem.org/mediawiki/2018/f/f0/T--Nanjing-China--i-background-8.png" width="85%" />
 
−
  <p>Iron-only  <br />(FeFe)-dependent</p></div>
 
−
  </div>
 
−
  <div class="word">
 
−
  <div class="word-background-block"></div><h4>MoFe has been studied extensively</h4>
 
−
  </div>
 
−
    <div class="word">
 
−
  <div class="word-background-block"></div><h3>Nitrogen Fixation Gene Cluster </h3>
 
−
  <img src="https://static.igem.org/mediawiki/2018/d/d5/T--Nanjing-China--i-background-9.png" width="85%" />
 
−
  <h4>The minimal nif genes required for nitrogen fixation.</h4>
 
−
  </div>
 
−
  </div>
 
−
  <div id="principle">
 
−
  <div class="word">
 
−
      <h2>General principle of biological nitrogen fixation </h2>
 
−
    <table width="90%" border="1" cellspacing="1" cellpadding="1">
 
−
      <tr>
 
−
        <td><font color="#0033CC">01 Hydrolysis of ATP</font></td>
 
−
        <td rowspan="2" width="65%"><div class="word-background-block" style="alignment-adjust:central"><img src="https://static.igem.org/mediawiki/2018/2/22/T--Nanjing-China--i-background-10.png" width="100%"/></div>
 
−
          <p align="center">Chemical energy (ATP)→Solar energy</p></td>
 
−
        <td><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
 
−
      </tr>
 
−
      <tr>
 
−
        <td><font color="#CCCC00">02 Electron transfer </font></td>
 
−
        <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
 
−
      </tr>
 
−
    </table>
 
−
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−
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−
    <div class="word-background-block"></div>
 
−
      <table width="90%" border="1" cellspacing="1" cellpadding="1">
 
−
      <tr>
 
−
        <td><font color="#0033CC">01 Receive light signals</font></td>
 
−
        <td rowspan="2" width="65%" style="alignment-adjust:central"><img src="https://static.igem.org/mediawiki/2018/e/e3/T--Nanjing-China--i-background-11.png" width="100%"/>
 
−
        </td>
 
−
        <td><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
 
−
      </tr>
 
−
      <tr>
 
−
        <td><font color="#CCCC00">02 Electron transfer </font></td>
 
−
        <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
 
−
      </tr>
 
−
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−
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−
    </div>
 
−
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−
  <div class="word">
 
−
      <h2>The characteristics of whole-cell</h2>
 
−
  <p><font size="110%" color="#CC6633">The property and advantage of whole-cells</font></p>
 
−
  <p>•Fast  proliferation to enable large-scale application<br />
 
−
  •Good  portability to permit on-site monitoring<br />
 
−
  •Inexpensive  and easy to preserve<br />
 
−
  •  Intracellular cascade reactions to amplify signals<br/>
 
−
  •  Excellent specificity, sensitivity and stability</p>
 
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               P.R. of China<br />
 
               P.R. of China<br />
 
               Zip: 210046
 
               Zip: 210046
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          <div class="f-word">
 
             <p><strong><u>Email</u></strong></p>
 
             <p><strong><u>Email</u></strong></p>
 
             <p>nanjing_china@163.com</p>
 
             <p>nanjing_china@163.com</p>
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Latest revision as of 02:31, 18 October 2018

Nanjing-China2018

Abstract

Hello, everyone! We are a group of students with everlasting passion and perseverance from Nanjing University, and we can’t wait to share with you those stories of our incredible iGEM team!

We have been on our journey of iGEM as sophomores and seniors since 2017. Though, at the very beginning, most of us were not so familiar with synthetic biology and relevant experimental skills in molecular biology, we still decided to embrace this challenging yet truly rewarding competition, and it turns out that this is the wisest decision we have ever made, for not only does the competition grant us with valuable experiences of scientific researches, but also helps us establish friendship with team members, a sense of cooperation, and the enthusiasm for science, which shall never fade from our memories.

Our team consists of students from Life Science Department, all of whom are nice colleagues to work with and interesting souls to communicate with. We inspire each other a lot in every aspect during the whole process—from seeking for innovative ideas, operating complicated experiments, carrying out human practices, designing wiki and posters to present our work completely at last. Tremendous support is also consistently and nicely offered by our enthusiastic advisers, instructors and sponsors. Genuinely, we thank you all, for we couldn’t have enjoyed doing this without the effort made by every one of you!

☟(Check below to see our teammates, advisors and instructors!)

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china