Difference between revisions of "Team:TecMonterrey GDL/Parts"

 
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{{TecMonterrey_GDL}}
 
{{TecMonterrey_GDL}}
 
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        <h1><img class="img-decorator2" alt="decoration2" src="https://static.igem.org/mediawiki/2018/3/3a/T--TecMonterrey_GDL--decoration1.png">Mathematical Model <img class="img-decorator" alt="decoration1" src="https://static.igem.org/mediawiki/2018/3/3a/T--TecMonterrey_GDL--decoration1.png"></h1>
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                    <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE">Genetic engineering</a></li>
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<!--End Main Header -->
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<section class="page-title text-center" style="background-image:url(https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Parts--ENCABEZADO.png);">
 +
    <div class="container">
 +
        <div class="title-text">
 +
            <h1>Parts</h1>
 +
        </div>
 
     </div>
 
     </div>
 +
</section>
  
  <div class="cool-container">
 
        <div class="cool-wrapper">
 
            <div class="internal-wrapper">
 
                <div class="row">
 
                    <div class="col smart-headers">
 
                        <h1 align="center"> <span>Parts</span> </h1>
 
<div>
 
<h3 class="writing">In the following table we present the parts we have used for our plasmid construction, detailed in Constructs, the parts we used are classified as they appear in our composite constructs:
 
</h3>
 
<h4 class="writing"> For E: Coli </h4>
 
</div>
 
  
<h3 align="right"> Strategy A:  E. coli
+
<section class="service-overview section">
</h3>
+
    <div class="container">
<img src="https://static.igem.org/mediawiki/2018/f/f1/T--TecMonterrey_GDL--Construct3.png" align="right">
+
        <div class="row">
<h1></h1>
+
<h3 align="left"> Simbology </h3>
+
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
<h1></h1>
+
<h1></h1>
+
<div>
+
<h2 style="color:#00008B" align="center" > Table A. Strategy A:  E. coli  </h2>
+
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
<img src="https://static.igem.org/mediawiki/2018/c/cb/T--TecMonterrey_GDL--Table1.png">
+
<h1></h1>
+
</div>
+
<div>
+
<h3 align="center"> Strategy B:  E. coli
+
</h3>
+
<img src="https://static.igem.org/mediawiki/2018/c/c1/T--TecMonterrey_GDL--ConstructReal2.png" align="center">
+
<h1></h1>
+
<h3 align="left"> Simbology </h3>
+
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
<h1></h1>
+
<h1></h1>
+
    <div>
+
<h2 style="color:#00008B" align="center" > Table B. Strategy A:  E. coli  </h2>
+
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
<img src="https://static.igem.org/mediawiki/2018/2/2d/T--TecMonterrey_GDL--Table2.png">
+
</div>
+
</div>
+
  
    <div>
+
          <div class="col-md-12">
<h3 align="left"> For L. rhamnosus GG:
+
            <div class="contents">
</h3>
+
                <div class="section-title">
<h3 align="right> Strategy A: L. rhamnosus GG
+
                    <h3>For <i>E.coli</i></h3>
</h3>
+
                </div>
<img src="https://static.igem.org/mediawiki/2018/c/c1/T--TecMonterrey_GDL--ConstructReal2.png" align="right">
+
                <div class="text">
<h1></h1>
+
                    <p style="text-align:center; font-size: 18px;">
<h3 align="left"> Simbology </h3>
+
                      In the following table we present the parts we have used for our plasmid construction, detailed in
<img src="https://static.igem.org/mediawiki/2018/4/48/T--TecMonterrey_GDL--Construct5.png" align="left">
+
                      <a href="https://2018.igem.org/Team:TecMonterrey_GDL/Composite_Part">Construct design</a>, the parts we used are classified as they appear in our composite constructs:
<h1></h1>
+
                    </p>
<h1></h1>
+
                </div>
    <div>
+
              </div>
<h2 style="color:#00008B" align="center" > Table B. Strategy A: E. coli </h2>
+
              <div class="row">
<h3 style="color:#00008B" align="center"> (all optimized for E. coli)</h3>
+
                <center>
<img src="https://static.igem.org/mediawiki/2018/2/2d/T--TecMonterrey_GDL--Table2.png">
+
                  <br>
</div>
+
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b6/T--TecMonterrey_GDL--Parts--2.png" alt="service-image">
</div>
+
                </center>
 +
              </div>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/31/T--TecMonterrey_GDL--Parts--Tabla1.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br><br>
 +
                  <center>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760010:Design#Source">Strategy A : Part:BBa_K2760010 - Lactobachill Plan A (E. coli)</a></h4>
 +
                </center><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/c2/T--TecMonterrey_GDL--Parts--1.png" alt="service-image">
  
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K206000">BBa_K206000</a>, <a href="http://parts.igem.org/Part:BBa_J34801">BBa_J34801</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760002">BBa_K2760002</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a></p>
 +
                </div>
 +
              </div>
  
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br><br><br><br>
 +
                  <center>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760011">Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill</a></h4>
 +
                </center><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e0/T--TecMonterrey_GDL--Parts--3.png" alt="service-image">
  
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K206000">BBa_K206000</a>, <a href="http://parts.igem.org/Part:BBa_J34801">BBa_J34801</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760003">BBa_K2760003</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/d/de/T--TecMonterrey_GDL--Parts--Tabla2.png" alt="service-image">
 +
                </div>
 +
              </div>
  
 +
              <br><br>
 +
                  <div class="section-title">
 +
                      <h3>For <i>L. rhamnosus GG</i></h3>
 +
                  </div>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/84/T--TecMonterrey_GDL--Parts--Tabla3.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br><br><br><br><br><br>
 +
                  <center>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760033">Strategy A:  Part: BBa_K2760033</a></h4>
 +
                </center><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/bc/T--TecMonterrey_GDL--Parts--4.png" alt="service-image">
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>,<a href="http://parts.igem.org/Part:BBa_K2760002">BBa_K2760002</a> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760008">BBa_K2760008</a> </p>
 +
                </div>
 +
              </div>
 +
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
 +
                  <center>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760034">Plan B (L. rhamnosus): Part: BBa_K276004</a></h4>
 +
                </center><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/42/T--TecMonterrey_GDL--Parts--5.png" alt="service-image">
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a>, <a href="http://parts.igem.org/Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760003">BBa_K2760003</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760008">BBa_K2760008</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/7/7d/T--TecMonterrey_GDL--Parts--Tabla4.png" alt="service-image">
 +
                </div>
 +
              </div>
 +
 +
              <br><br><br>
 +
              <div class="service-box section-title text-center">
 +
                  <h3> <u>Signaling peptides </u>
 +
                  </h3>
 +
                  <p style="text-align:center">For the excretion of recombinant proteins</p>
 +
              </div>
 +
              <br><br><br>
 +
              <div class="section-title">
 +
                  <h3>For <i>E.coli</i></h3>
 +
              </div>
 +
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e6/T--TecMonterrey_GDL--Parts--Tabla5.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
<br><br>
 +
                  <h4><a style="text-align:center" href="http://parts.igem.org/Part:BBa_K2760019:Design#References">Sp1-Nsp4 construct: BBa_K2760019 -
 +
Characterization construct for SGP1-NSP4 -</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/33/T--TecMonterrey_GDL--Parts--6.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4><br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Parts--Tabla6.png" alt="service-image">
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b5/T--TecMonterrey_GDL--Parts--Tabla7.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br>
 +
                  <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 -  Negative control for signal peptide characterization</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
              </div>
 +
              <br><br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 -  Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/31/T--TecMonterrey_GDL--Parts--Tabla8.png" alt="service-image">
 +
                </div>
 +
              </div>
 +
 +
              <br><br><br>
 +
              <div class="section-title">
 +
                  <h3>For <i>L.rhamnosus</i></h3>
 +
              </div>
 +
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/1a/T--TecMonterrey_GDL--Parts--Tabla9.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p>
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br><br><br><br><br><br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p>
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/6/66/T--TecMonterrey_GDL--Parts--Tabla10.png" alt="service-image">
 +
                </div>
 +
              </div>
 +
              <br><br>
 +
              <div class="row">
 +
                <div class="col-md-6">
 +
                  <br>
 +
                  <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/cf/T--TecMonterrey_GDL--Parts--Tabla11.png" alt="service-image">
 +
                </div>
 +
                <div class="col-md-6">
 +
                  <br><br><br>
 +
                  <h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4>
 +
                <br>
 +
<center>
 +
                  <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image">
 +
</center>
 +
 +
                  <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>,
 +
                    <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p>
 +
                </div>
 +
              </div>
 +
        </div>
 +
    </div>
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Latest revision as of 02:34, 18 October 2018

TecMonterrey_GDL

Parts

For E.coli

In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:


service-image


For L. rhamnosus GG




Signaling peptides

For the excretion of recombinant proteins




For E.coli











For L.rhamnosus