In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:
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+ | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Attributions">Our team</a></li> | ||
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+ | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Model">Mathematical model</a></li> | ||
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+ | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE">Genetic engineering</a></li> | ||
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+ | <li role="separator" class="divider"></li> | ||
+ | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Gut-on-a-chip">Gut on a chip</a></li> | ||
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+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">What's next?<span class="caret"></span></a> | ||
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+ | <!--End Main Header --> | ||
+ | <section class="page-title text-center" style="background-image:url(https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Parts--ENCABEZADO.png);"> | ||
+ | <div class="container"> | ||
+ | <div class="title-text"> | ||
+ | <h1>Parts</h1> | ||
+ | </div> | ||
</div> | </div> | ||
+ | </section> | ||
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− | + | <div class="col-md-12"> | |
− | + | <div class="contents"> | |
− | </ | + | <div class="section-title"> |
− | <img src="https://static.igem.org/mediawiki/2018/ | + | <h3>For <i>E.coli</i></h3> |
− | < | + | </div> |
− | < | + | <div class="text"> |
− | <img src="https://static.igem.org/mediawiki/2018/ | + | <p style="text-align:center; font-size: 18px;"> |
− | < | + | In the following table we present the parts we have used for our plasmid construction, detailed in |
− | < | + | <a href="https://2018.igem.org/Team:TecMonterrey_GDL/Composite_Part">Construct design</a>, the parts we used are classified as they appear in our composite constructs: |
− | + | </p> | |
− | < | + | </div> |
− | < | + | </div> |
− | <img src="https://static.igem.org/mediawiki/2018/ | + | <div class="row"> |
− | + | <center> | |
− | + | <br> | |
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b6/T--TecMonterrey_GDL--Parts--2.png" alt="service-image"> | ||
+ | </center> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/31/T--TecMonterrey_GDL--Parts--Tabla1.png" alt="service-image"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br><br><br> | ||
+ | <center> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760010:Design#Source">Strategy A : Part:BBa_K2760010 - Lactobachill Plan A (E. coli)</a></h4> | ||
+ | </center><br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/c2/T--TecMonterrey_GDL--Parts--1.png" alt="service-image"> | ||
− | + | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K206000">BBa_K206000</a>, <a href="http://parts.igem.org/Part:BBa_J34801">BBa_J34801</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, | |
− | + | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760002">BBa_K2760002</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a></p> | |
− | + | </div> | |
− | < | + | </div> |
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− | </ | + | |
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− | < | + | |
− | < | + | |
− | </div> | + | |
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br><br><br><br><br> | ||
+ | <center> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760011">Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill</a></h4> | ||
+ | </center><br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e0/T--TecMonterrey_GDL--Parts--3.png" alt="service-image"> | ||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K206000">BBa_K206000</a>, <a href="http://parts.igem.org/Part:BBa_J34801">BBa_J34801</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760003">BBa_K2760003</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a></p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/d/de/T--TecMonterrey_GDL--Parts--Tabla2.png" alt="service-image"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div class="section-title"> | ||
+ | <h3>For <i>L. rhamnosus GG</i></h3> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/8/84/T--TecMonterrey_GDL--Parts--Tabla3.png" alt="service-image"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br><br><br><br><br><br><br> | ||
+ | <center> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760033">Strategy A: Part: BBa_K2760033</a></h4> | ||
+ | </center><br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/bc/T--TecMonterrey_GDL--Parts--4.png" alt="service-image"> | ||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>,<a href="http://parts.igem.org/Part:BBa_K2760002">BBa_K2760002</a> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760008">BBa_K2760008</a> </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br><br><br><br><br><br><br><br><br><br><br> | ||
+ | <center> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760034">Plan B (L. rhamnosus): Part: BBa_K276004</a></h4> | ||
+ | </center><br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/4/42/T--TecMonterrey_GDL--Parts--5.png" alt="service-image"> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760023">BBa_K2760023</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a>, <a href="http://parts.igem.org/Part:BBa_K216005">BBa_K216005</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760003">BBa_K2760003</a>, | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760008">BBa_K2760008</a></p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/7/7d/T--TecMonterrey_GDL--Parts--Tabla4.png" alt="service-image"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <br><br><br> | ||
+ | <div class="service-box section-title text-center"> | ||
+ | <h3> <u>Signaling peptides </u> | ||
+ | </h3> | ||
+ | <p style="text-align:center">For the excretion of recombinant proteins</p> | ||
+ | </div> | ||
+ | <br><br><br> | ||
+ | <div class="section-title"> | ||
+ | <h3>For <i>E.coli</i></h3> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/e/e6/T--TecMonterrey_GDL--Parts--Tabla5.png" alt="service-image"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br> | ||
+ | <h4><a style="text-align:center" href="http://parts.igem.org/Part:BBa_K2760019:Design#References">Sp1-Nsp4 construct: BBa_K2760019 - | ||
+ | Characterization construct for SGP1-NSP4 -</a></h4> | ||
+ | <br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/33/T--TecMonterrey_GDL--Parts--6.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4><br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Parts--Tabla6.png" alt="service-image"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/b/b5/T--TecMonterrey_GDL--Parts--Tabla7.png" alt="service-image"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br> | ||
+ | <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 - Negative control for signal peptide characterization</a></h4> | ||
+ | <br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br><br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4> | ||
+ | <br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/31/T--TecMonterrey_GDL--Parts--Tabla8.png" alt="service-image"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <br><br><br> | ||
+ | <div class="section-title"> | ||
+ | <h3>For <i>L.rhamnosus</i></h3> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/1/1a/T--TecMonterrey_GDL--Parts--Tabla9.png" alt="service-image"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br><br> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4> | ||
+ | <br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br><br><br><br> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4> | ||
+ | <br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/6/66/T--TecMonterrey_GDL--Parts--Tabla10.png" alt="service-image"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div class="row"> | ||
+ | <div class="col-md-6"> | ||
+ | <br> | ||
+ | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/c/cf/T--TecMonterrey_GDL--Parts--Tabla11.png" alt="service-image"> | ||
+ | </div> | ||
+ | <div class="col-md-6"> | ||
+ | <br><br><br> | ||
+ | <h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4> | ||
+ | <br> | ||
+ | <center> | ||
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image"> | ||
+ | </center> | ||
+ | |||
+ | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, | ||
+ | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | ||
+ | </div> | ||
+ | </div> | ||
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Latest revision as of 02:34, 18 October 2018
Parts
For E.coli
Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill
Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760003, BBa_K2760012, BBa_K2760029
For L. rhamnosus GG
Plan B (L. rhamnosus): Part: BBa_K276004
Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760006, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000, BBa_K2760003, BBa_K2760012, BBa_K2760029, BBa_K2760008
Signaling peptides
For the excretion of recombinant proteins
For E.coli
BBa_K2760021 - SP2-Nsp2 Characterization construct
Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760004, BBa_K2760024, BBa_K2760012, BBa_B0015
BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)
Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760001, BBa_K2760024, BBa_K2760012, BBa_B0015
For L.rhamnosus
Sp2-Nsp2- Characterization Construct Part: BBa_K2760031
Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760004, BBa_K2760029, BBa_K2760007