In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:
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<li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Abstract">Abstract</a></li> | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Abstract">Abstract</a></li> | ||
<li role="separator" class="divider"></li> | <li role="separator" class="divider"></li> | ||
− | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/ | + | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Attributions">Our team</a></li> |
<li role="separator" class="divider"></li> | <li role="separator" class="divider"></li> | ||
− | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/ | + | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Partners">Partners</a></li> |
<li role="separator" class="divider"></li> | <li role="separator" class="divider"></li> | ||
<li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Checklist">Checklist</a></li> | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Checklist">Checklist</a></li> | ||
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</li> | </li> | ||
<li class="dropdown "> | <li class="dropdown "> | ||
− | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Dry | + | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Dry lab<span class="caret"></span></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Parts">Parts</a></li> | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Parts">Parts</a></li> | ||
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<a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results<span class="caret"></span></a> | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results<span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
− | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE"> | + | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE">Genetic engineering</a></li> |
<li role="separator" class="divider"></li> | <li role="separator" class="divider"></li> | ||
<li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Demonstrate">Biomimetric Tri-cultures</a></li> | <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Demonstrate">Biomimetric Tri-cultures</a></li> | ||
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</div> | </div> | ||
<div class="text"> | <div class="text"> | ||
− | <p> | + | <p style="text-align:center; font-size: 18px;"> |
In the following table we present the parts we have used for our plasmid construction, detailed in | In the following table we present the parts we have used for our plasmid construction, detailed in | ||
− | <a href=" | + | <a href="https://2018.igem.org/Team:TecMonterrey_GDL/Composite_Part">Construct design</a>, the parts we used are classified as they appear in our composite constructs: |
</p> | </p> | ||
</div> | </div> | ||
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<br><br><br> | <br><br><br> | ||
− | <div class="section-title text-center"> | + | <div class="service-box section-title text-center"> |
− | <h3>Signaling peptides | + | <h3> <u>Signaling peptides </u> |
</h3> | </h3> | ||
− | <p>For the excretion of recombinant proteins</p> | + | <p style="text-align:center">For the excretion of recombinant proteins</p> |
</div> | </div> | ||
<br><br><br> | <br><br><br> | ||
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</div> | </div> | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
− | <h4><a href="http://parts.igem.org/Part:BBa_K2760019:Design#References">Sp1-Nsp4 construct: BBa_K2760019 - | + | <br><br> |
+ | <h4><a style="text-align:center" href="http://parts.igem.org/Part:BBa_K2760019:Design#References">Sp1-Nsp4 construct: BBa_K2760019 - | ||
Characterization construct for SGP1-NSP4 -</a></h4> | Characterization construct for SGP1-NSP4 -</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/33/T--TecMonterrey_GDL--Parts--6.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/33/T--TecMonterrey_GDL--Parts--6.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760000">BBa_K2760000</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
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<div class="col-md-6"> | <div class="col-md-6"> | ||
<br> | <br> | ||
− | <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4> | + | <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4><br> |
− | + | <center> | |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image"> | + | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image"> |
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
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<br><br> | <br><br> | ||
<h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 - Negative control for signal peptide characterization</a></h4> | <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 - Negative control for signal peptide characterization</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
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<div class="col-md-6"> | <div class="col-md-6"> | ||
<h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4> | <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
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<br><br><br><br><br><br> | <br><br><br><br><br><br> | ||
<h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4> | <h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, | ||
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<br><br><br><br><br><br> | <br><br><br><br><br><br> | ||
<h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4> | <h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | ||
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<br><br><br> | <br><br><br> | ||
<h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4> | <h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, |
Latest revision as of 02:34, 18 October 2018
Parts
For E.coli
Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill
Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760003, BBa_K2760012, BBa_K2760029
For L. rhamnosus GG
Plan B (L. rhamnosus): Part: BBa_K276004
Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760006, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000, BBa_K2760003, BBa_K2760012, BBa_K2760029, BBa_K2760008
Signaling peptides
For the excretion of recombinant proteins
For E.coli
BBa_K2760021 - SP2-Nsp2 Characterization construct
Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760004, BBa_K2760024, BBa_K2760012, BBa_B0015
BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)
Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760001, BBa_K2760024, BBa_K2760012, BBa_B0015
For L.rhamnosus
Sp2-Nsp2- Characterization Construct Part: BBa_K2760031
Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760004, BBa_K2760029, BBa_K2760007