Difference between revisions of "Team:UCSC/Attributions"

 
(57 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{:Team:UCSC/NavBar}}
+
{{:Team:UCSC/NavBar-2}}
  
 
<html>
 
<html>
  
 
<style>
 
<style>
 +
 +
.float-toc {
 +
  top: calc(45px + 15vh);
 +
}
 +
 +
.float-toc a {
 +
  padding: calc((100vh - (150px + 84px) - 31px*17)/(2*17)) 1em;
 +
}
  
 
i {
 
i {
 
   font-size: 75%;
 
   font-size: 75%;
 
}
 
}
−
p-title {
+
 
−
font-size: 80%;
+
h3 {
 +
  font-size: 1.5em !important;
 +
}
 +
 
 +
h6 {
 +
  color: #e91b63 !important;
 +
  font-size: 1.25em !important;
 +
  padding-top: 2em !important;
 +
  margin-top: 0 !important;
 
}
 
}
  
Line 15: Line 31:
  
 
<body style="overflow-x: visible;">
 
<body style="overflow-x: visible;">
−
<div class="toc-body">
+
<div class="content toc-body toc-right">
  
 
   <div class="header-image" id="header_image_attributions" style="background-image: url(https://static.igem.org/mediawiki/2018/c/c7/T--UCSC--Collab_Background.png); background-position: 40% 60%">
 
   <div class="header-image" id="header_image_attributions" style="background-image: url(https://static.igem.org/mediawiki/2018/c/c7/T--UCSC--Collab_Background.png); background-position: 40% 60%">
−
     <p class="header-title" style="top:50%;left:50%">
+
     <h1>
 
       Attributions
 
       Attributions
−
     </p>
+
     </h1>
 
   </div>
 
   </div>
  
 
   <!-- Include arrow to jump to top -->
 
   <!-- Include arrow to jump to top -->
−
   <a href="#top_row" class="scroll-to-top"><img src="https://static.igem.org/mediawiki/2018/5/58/T--UCSC--ArrowUp.png"></a>
+
   <a href="#top_anchor" class="scroll-to-top"><img src="https://static.igem.org/mediawiki/2018/c/cd/T--UCSC--Arrow.png"></a>
−
 
+
  
  
Line 31: Line 46:
  
 
<div class="float-toc">
 
<div class="float-toc">
−
   <li class="p-title"> Table of Contents </li>
+
   <div class="toc-container">
−
   <li><a href="#Idea"> The Project Idea </a></li>
+
  <li><h3 id="top_anchor"> Attributions </h3></li>
−
   <li><a href="#Team"> Official Team </a></li>
+
   <li><a href="#Attr_Idea"> The Project Idea </a></li>
−
   <li><a href="#Advi"> Advisors </a></li>
+
   <li><a href="#Attr_Team"> Official Team </a></li>
−
   <li><a href="#ResS"> Research Support </a></li>
+
   <li><a href="#Attr_Advi"> Advisors </a></li>
−
   <li><a href="#LabS"> Lab Support </a></li>
+
   <li><a href="#Attr_ResS"> Research Support </a></li>
−
   <li><a href="#WikS"> Wiki Support </a></li>
+
   <li><a href="#Attr_LabS"> Lab Support </a></li>
−
   <li><a href="#HPS"> Human Practices Support </a></li>
+
   <li><a href="#Attr_WikS"> Wiki Support </a></li>
−
   <li><a href="#FndS"> Funding Support </a></li>
+
   <li><a href="#Attr_HPS"> Human Practices Support </a></li>
−
   <li><a href="#IndS"> Industry Support </a></li>
+
   <li><a href="#Attr_FndS"> Funding Support </a></li>
−
   <li><a href="#NPP"> Non-Profit Partnerships </a></li>
+
   <li><a href="#Attr_IndS"> Industry Support </a></li>
−
   <li><a href="#DTS"> Difficult Technique Support </a></li>
+
   <li><a href="#Attr_NPP"> Non-Profit Partnerships </a></li>
−
   <li><a href="#PrsS"> Presentation Support </a></li>
+
   <li><a href="#Attr_PrsS"> Presentation Support </a></li>
−
   <li><a href="#GenS"> General Support </a></li>
+
   <li><a href="#Attr_GenS"> General Support </a></li>
 +
   </div>
 
</div>
 
</div>
  
Line 50: Line 66:
  
  
−
   <div class="flex-row" id="top_row">
+
   <div class="flex-row">
 
     <div class="flex-col">
 
     <div class="flex-col">
−
       <p class="p-title">Thank You</p>
+
       <h2> Thank You </h2>
−
       <p>The PoPPY team would like to thank everyone who has helped make this project possible. For the past five years, undergraduates at UCSC have participated in iGEM. This year, the team is composed of students studying Bioengineering, Bioinformatics, Bioengineering (Assistive Technology: Motor), Molecular, Cellular, and Developmental Biology, Marine Biology, and Cognitive Science: Artificial Intelligence and Human Computer Interaction. Our diverse group of individuals is crucial for the creative process behind using synthetic biology to help others. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2018 UCSC iGEM team is creating effective solutions and developing models for future success.</p>
+
       <p><b>The PoPPY team would like to thank everyone who made this project possible</b>, especially those described below. For the past five years, undergraduates at UCSC have participated in iGEM. This year, the team is composed of students studying Bioengineering (Biomolecular), Bioinformatics, Bioengineering (Assistive Technology: Motor), Molecular, Cellular, and Developmental Biology, Marine Biology, and Cognitive Science: Artificial Intelligence and Human Computer Interaction. Our diverse group of individuals is crucial for the creative process behind using synthetic biology to help others. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2018 UCSC iGEM team is solving worldwide issues and developing methods to expand their research in the future.</p>
 
     </div>
 
     </div>
  
Line 63: Line 79:
 
     </div>
 
     </div>
 
   </div>
 
   </div>
−
<br>
 
  
  
Line 72: Line 87:
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="Idea"> The Project Idea </p>
+
    <h3 id="Attr_Idea"> The Project Idea </h3>
−
 
+
    <p> After several journal club meetings, our team determined the PoPPY project was the most meaningful pursuit. The initial idea was proposed by captain <b><u><a href="https://2018.igem.org/Team:UCSC/Team#morgan" style="text-decoration: none; color: black;">Morgan Tardy</a></u></b> and mentor <b><u><a href="https://2018.igem.org/Team:UCSC/Team#mckenna" style="text-decoration: none; color: black;">McKenna Hicks</a></u></b>.</p>
−
 
+
  </div>
−
<p> We came to the idea of PoPPY after many journal club meetings including all team members. The initial idea was proposed by captain <b><a href="https://2018.igem.org/Team:UCSC/Team#morgan" style="text-decoration: none; color: black;">Morgan Tardy</a></b> and was eventually agreed upon by the entire team. </p>
+
−
</div>
+
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
 
  
  
Line 89: Line 97:
 
<!--      *********************************** Students *************************************      -->
 
<!--      *********************************** Students *************************************      -->
 
<!--      **********************************************************************************      -->
 
<!--      **********************************************************************************      -->
−
<br>
 
−
<br>
 
−
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="Team">Official Team</p>
+
    <h3 id="Attr_Team">Official Team</h3>
−
 
+
Thank you to all the 2018 UCSC iGEM Team members for their hard work and dedication to the PoPPY project's message. Check out our <b><u><a href="https://2018.igem.org/Team:UCSC/Team" style="text-decoration: none; color: black;">Meet the Team page</a></u></b> to learn about each teammate.
−
 
+
  </div>
−
</div>
+
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
 
  
  
Line 110: Line 109:
 
<!--      *********************************** Advisors *************************************      -->
 
<!--      *********************************** Advisors *************************************      -->
 
<!--      **********************************************************************************      -->
 
<!--      **********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="Advi">Advisors</p>
+
    <h3 id="Attr_Advi">Advisors</h3>
 +
    <h6><b> David Bernick, </b>
 +
        <i> Assistant Adjunct Professor, University of California Santa Cruz, Department of Biomolecular Engineering </i></h6>
 +
    <p>Dr. Bernick for guided our team and offered valuable insight and advice through each stage of the project. He helped our team manage funding, finances and logistical tasks.</p>
 +
    <br>
  
 +
    <h6><b> McKenna Hicks, </b>
 +
        <i> Master's Student, University of California Santa Cruz, Department of Biomolecular Engineering </i></h6>
 +
    <p>McKenna for provided advice on gene design and wet lab experiments.</p>
  
−
<p class="p-title">
+
<h6><b> Todd Lowe, </b>  
−
  <b> Dr. David Bernick, </b>  
+
        <i> Professor, University of California Santa Cruz, Department of Biomolecular Engineering </i></h6>
−
  <i> Assistant Adjunct Professor, University of California Santa Cruz, Department of Biomolecular Engineering </i>
+
    <p>Todd Lowe acted as our assistant advisor and provided advice on coding for our website.</p>
−
</p>
+
−
<p>
+
−
  Guided our team and offered valuable insight and advice through each stage of the project. He also helped the team manage
+
−
  funding, finances and logistical tasks.
+
−
</p><br>
+
  
−
 
+
   </div>
−
<p class="p-title">
+
−
   <b> Mckenna Hicks, </b>
+
−
  <i> Master's Student, University of California Santa Cruz, Department of Biomolecular Engineering </i>
+
−
</p>
+
−
<p>
+
−
  Guided us and provided advice on gene design and wet lab experiments
+
−
</p><br>
+
 
</div>
 
</div>
−
</div>
 
−
 
−
 
−
 
−
 
−
 
−
<br>
 
−
<br>
 
−
  <div class="flex-row">
 
−
    <div class="content-divider">
 
−
      <img src="https://static.igem.org/mediawiki/2018/d/d9/T--UCSC--FlowerDivider.png" alt="divider image">
 
−
    </div>
 
−
  </div>
 
−
<br>
 
−
<br>
 
−
<br>
 
−
 
−
 
  
  
Line 161: Line 135:
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
 +
    <h3 id="Attr_ResS">Research Support</h3>
 +
<h6>
 +
  <b> Hal Alper, </b>
 +
  <i> Professor, University of Texas at Austin, Department of Chemical Engineering </i> 
 +
</h6>
 +
<p>
 +
  Professor Alper provided recommendations for Tsynth27/Tsynth8 terminators and confirmed which promoters would be necessary for our project. Through a Materials Transfer Agreement, Alper sent us plasmids carrying terminators and fluorescent markers to test for gene activity.
 +
</p>
 +
 
 +
<h6>
 +
  <b> William Belden, </b>
 +
  <i> Associate Professor, Rutgers University, Department of Animal Sciences </i> 
 +
</h6>
 +
<p>
 +
  Professor Belden provided valuable information on yeast-mediated cloning. Professor Belden inspired our parallel experiment design and even offered to help design our flanking oligos.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Erin Bredeweg, </b>
 +
  <i> Biosystems Dynamics & Simula Scientist, Pacific Northwest National Laboratory </i>
 +
</h6>
 +
<p>
 +
  Erin Bredeweg assisted us in finding sequence information on our <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> strain.
 +
</p>
  
−
<p style="font-size:150% !important" class="week" id="ResS">Research Support</p>
+
<h6>
−
<p class="p-title">
+
 
   <b> Tony Carr, </b>  
 
   <b> Tony Carr, </b>  
 
   <i> Professor of Molecular Genetics, University of Sussex, Genome Damage and Stability, Genome Damage and Stability Centre </i>  
 
   <i> Professor of Molecular Genetics, University of Sussex, Genome Damage and Stability, Genome Damage and Stability Centre </i>  
 +
</h6>
 +
<p>
 +
  Professor Carr answered our questions regarding the size of genomic insert, directionality of Lox sites, and cre-lox recombination.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> Catherine Duport, </b>
 +
  <i> University of Avignon, Microbiotechnology, Ecotoxicology, and Bioremediation </i>
 +
</h6>
 
<p>
 
<p>
−
   Answered questions regarding size of genomic insert, directionality of lox sites, and cre-lox.
+
   Catherine Duport helped clarify pieces of her 1998 experiment such as why certain promoters and genes were chosen. Duport also attempted to find us a <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> strain.
−
</p><br>
+
</p>
  
−
<p class="p-title">
+
<h6>
−
   <b> Stefan Tippmann, </b>  
+
   <b> Daniel Gallie, </b>  
−
   <i> Visiting Researcher, Chalmers University, Life Science Engineering </i>
+
   <i> Professor, University of California Riverside, Biochemistry Department </i>
 +
</h6>
 +
<p>
 +
  Professor Gallie sent us an electroporation protocol with mRNA for reference.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> Marc Gartenberg, </b>
 +
  <i> Professor, Rutgers University, Department of Biochemistry and Molecular Biology </i> 
 +
</h6>
 
<p>
 
<p>
−
   Clarified Questions on yeast in general and provided us with additional readings to look through.
+
   Professor Gartenberg provided information on parts of pXRU3/L2 plasmid including HOp region, CEN/ARS regions, pRS marker, and how to insert Lox sites into these particular plasmids.  
−
</p><br>
+
</p>
  
 +
<h6>
 +
  <b> Rohinton Kamakaka, </b>
 +
  <i> Professor, University of California Santa Cruz, Department of Molecular Cellular and Developmental Biology </i> 
 +
</h6>
 +
<p>
 +
  Professor Kamakaka sent us protocols on yeast-mediated cloning. He also answered questions on growing and maintaining yeast and even provided us a <i style="font-size:100% !important">Saccharomyces Cerevisiae</i> strain for yeast-mediated cloning. Professor Kamakaka has also provided general support and advice for all our experiments.
 +
</p>
  
−
<p class="p-title">
+
<h6>
 
   <b> Oliver Konzock, </b>  
 
   <b> Oliver Konzock, </b>  
 
   <i> PhD Student, Chalmers University, Life Science Engineering </i>  
 
   <i> PhD Student, Chalmers University, Life Science Engineering </i>  
 +
</h6>
 +
<p>
 +
  Oliver Konzock answered questions on how to grow yeast (and care for <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i>). He also answered questions on how to successfully perform yeast-mediated cloning.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> Jah Nehlin, </b>
 +
  <i> Copenhagen University Hospital Hvidovre </i> 
 +
</h6>
 
<p>
 
<p>
−
   Answered questions general questions on how to grow yeast and yarrowia lipolytica. Answered questions on how to do yeast mediated cloning.
+
   Mehlin gave insight into how the GAL1 promoter works.
−
</p><br>
+
</p>
−
 
+
  
−
<p class="p-title">
+
<h6>
 
   <b> Jean-Marc Nicaud, </b>  
 
   <b> Jean-Marc Nicaud, </b>  
 
   <i> Directeur de Recherche INRA </i>  
 
   <i> Directeur de Recherche INRA </i>  
−
</p>
+
</h6>
 
<p>
 
<p>
−
  Introduced us to Golden Gate Assembly method. Answered questions building plasmids. Showed us M1G1 wouldn't be an issue.
+
  Jean-Marc Nicaud introduced us to the Golden Gate Assembly method. He also answered questions about building plasmids and showed us M1G1 wouldn't be an issue in terms of our promoter/terminator design.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Adam Watson, </b>
+
−
  <i> Research Fellow, University of Sussex, Genome Damage and Stability </i> 
+
 
</p>
 
</p>
−
<p>
 
−
  Answered questions regarding size of genomic insert, directionality of lox sites, and cre-lox in general.
 
−
</p><br>
 
  
−
 
+
<h6>
−
<p class="p-title">
+
   <b> Saumya Ramarao, </b>  
−
   <b> Erin Bredeweg, </b>  
+
   <i> Senior Associate, Reproductive Health Supplies Coalition </i>
−
   <i> Biosystms Dynamics & Simula Scientist, Pacific Northwest National Laboratory </i>  
+
</h6>
−
</p>
+
 
<p>
 
<p>
−
   Assisted in location and finding sequence information of Yarrowia strain.
+
   Ramarao provided information regarding birth control production and needs.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Catherine Duport, </b>
+
−
  <i> University of Avignon, Microbiotechnology, Ecotoxicology, and Bioremediation </i>
+
 
</p>
 
</p>
−
<p>
 
−
  Helped clarify pieces of experiment such as why she used certain promoters. Attempted to find yarrowia strain.
 
−
</p><br>
 
  
−
 
+
<h6>
−
<p class="p-title">
+
   <b> Hans Ronne, </b>  
−
   <b> Saumya Ramarao, </b>  
+
   <i> Professor, Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology </i>   
−
   <i> Senior Associate, Reproductive Health Supplies Coalition </i>   
+
</h6>
−
</p>
+
 
<p>
 
<p>
−
   Provided information regarding birth control production and needs.
+
   Professor Ronne attempted to determine how to stop the binding of MIG1 to GALI promoter.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Hal Alper, </b>
+
−
  <i> Professor, The University of Texas at Austin, Department of Chemical Engineering </i> 
+
 
</p>
 
</p>
−
<p>
 
−
  Provided recommendations for Tsynth27 terminator and confirmed which promoters would be necessary for our project.
 
−
</p><br>
 
  
−
 
+
<h6>
−
<p class="p-title">
+
   <b> Cory Schwartz, </b>  
−
   <b> William Belden, </b>  
+
   <i> PhD Student, University of California Riverside, Department of Civil and Environmental Engineering </i>   
−
   <i> Associate Professor, Rutgers University, Department of Animal Sciences </i>   
+
</h6>
−
</p>
+
 
<p>
 
<p>
−
   Provided information on yeast-mediated cloning experiment. Offered to help design flanking oligos.
+
   Schwartz provided pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Peng Xu, </b>
+
−
  <i> University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering </i> 
+
 
</p>
 
</p>
−
<p>
 
−
  Proved Yarrowia Lipolytica could produce progesterone despite NHEJ preference. Showed that it is possible to use homologous recombination with Ku70 knockout Yarrowia Lipolytica strain.
 
−
</p><br>
 
−
 
  
−
<p class="p-title">
+
<h6>
 
   <b> Wendy Sun, </b>  
 
   <b> Wendy Sun, </b>  
 
   <i> University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering </i>   
 
   <i> University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering </i>   
 +
</h6>
 +
<p>
 +
  Sun assisted Xu in proving that <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> could produce progesterone.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> Stefan Tippmann, </b>
 +
  <i> Visiting Researcher, Chalmers University, Life Science Engineering </i>
 +
</h6>
 
<p>
 
<p>
−
  Proved Yarrowia Lipolytica could produce progesterone despite NHEJ preference. Showed that it is possible to use homologous recombination with Ku70 knockout Yarrowia Lipolytica strain.
+
Tippmann taught us about yeast in general and providing us with additional readings to learn about <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i>.
−
</p><br>
+
</p>
  
−
 
+
<h6>
−
<p class="p-title">
+
   <b> Adam Watson, </b>  
−
   <b> Marc Gartenberg, </b>  
+
   <i> Research Fellow, University of Sussex, Genome Damage and Stability </i>   
−
   <i> Professor, Rutgers University, Department of Biochemistry and Molecular Biology </i>   
+
</h6>
−
</p>
+
 
<p>
 
<p>
−
   Provided information on parts of pXRU3/L2 plasmid including HOp region, CEN/ARS regions, pRS marker, and how to insert lox sites into these particular plasmids.  
+
   Watson answered questions regarding the size of genomic inserts, directionality of Lox sites, and Cre-lox recombination.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Daniel Gallie, </b>
+
−
  <i> Professor, University of California Riverside, Biochemistry Department </i> 
+
 
</p>
 
</p>
−
<p>
 
−
  Sent electroporation protocol with mRNA for reference.
 
−
</p><br>
 
  
−
 
+
<h6>
−
<p class="p-title">
+
   <b> Ian Wheeldon, </b>  
−
   <b> Ian wheeldon, </b>  
+
 
   <i> Associate Professor, University of California Riverside, Department of Chemical and Environmental Engineering </i>   
 
   <i> Associate Professor, University of California Riverside, Department of Chemical and Environmental Engineering </i>   
−
</p>
+
</h6>
 
<p>
 
<p>
−
   Provided pHR_AO8_hrGFP plasmid sequence including homologous arm regions.
+
   Wheeldon provided the pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Cory Schwartz, </b>
+
−
  <i> PhD Student, University of California Riverside, Department of Civil and Environmental Engineering </i> 
+
 
</p>
 
</p>
−
<p>
 
−
  Provided pHR_AO8_hrGFP plasmid sequence including homologous arm regions.
 
−
</p><br>
 
  
−
 
+
<h6>
−
<p class="p-title">
+
   <b> Peng Xu, </b>  
−
   <b> Hans Ronne, </b>  
+
   <i> University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering </i>   
−
   <i> Professor, Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology </i>   
+
</h6>
−
</p>
+
 
<p>
 
<p>
−
   Helped attempt to determine how to stop the binding of Mig1 to Gal1 promoter.
+
   Xu proved that <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> could produce progesterone despite its NHEJ preference. He showed that it is possible to use homologous recombination with a KU70 knockout strain.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Jah Nehlin, </b>  
+
−
  <i> Copenhagen University Hospital Hvidovre </i>
+
 
</p>
 
</p>
−
<p>
 
−
  Gave insight into how Gal1 promoter works.
 
−
</p><br>
 
  
−
 
+
   </div>
−
<p class="p-title">
+
−
   <b> Rohinton Kamakaka, </b>
+
−
  <i> Professor, University of California Santa Cruz, Department of Molecular Cellular and Developmental Biology </i> 
+
−
</p>
+
−
<p>
+
−
  Sent protocol on yeast mediated cloning. Clarified questions on yeast and may provide Saccharomyces Cerevisiae strain.
+
−
</p><br>
+
−
 
+
−
 
+
−
 
+
−
</div>
+
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
  
  
Line 351: Line 296:
 
<!--      ********************************* Lab Support ************************************      -->
 
<!--      ********************************* Lab Support ************************************      -->
 
<!--      **********************************************************************************      -->
 
<!--      **********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="LabS"> Lab Support </p>
+
    <h3 id="Attr_LabS"> Lab Support </h3>
  
−
 
+
<h6>
−
<p class="p-title">
+
 
   <b> Dr. Hugh Olsen, </b>  
 
   <b> Dr. Hugh Olsen, </b>  
−
   <i> Lab Manager, University of California, Santa Cruz, Biomolecular Engineering Teaching Labs </i> </SPAN>  
+
   <i> Lab Manager, University of California, Santa Cruz, Biomolecular Engineering Teaching Labs </i>
 +
</h6>
 +
<p>
 +
  Dr. Olsen provided assistance with autoclave-training, general laboratory safety training, obtaining materials and maintaining lab equipment.
 
</p>
 
</p>
−
<p>
+
 
−
   Provided assistance with autoclave-training, general laboratory safety training, obtaining materials and maintaining equipment.
+
   </div>
−
</p><br>
+
−
</div>
+
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
  
  
Line 383: Line 316:
 
<!--      ********************************** Wiki Support *********************************      -->
 
<!--      ********************************** Wiki Support *********************************      -->
 
<!--      *********************************************************************************      -->
 
<!--      *********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="WikS">Wiki Support</p>
+
    <h3 id="Attr_WikS">Wiki Support</h3>
  
−
 
+
<h6>
−
<p class="p-title">
+
 
   <b> Evan Pepper, </b>  
 
   <b> Evan Pepper, </b>  
−
   <i> Former Undergraduate Student, University of California Santa Cruz, Biomolecular Engineering </i> </SPAN>  
+
   <i> Former Undergraduate Student, University of California Santa Cruz, Biomolecular Engineering </i>
 +
</h6>
 +
<p>
 +
  Evan provided advice on design choices and website organization.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> UCSC iGEM 2017 </b>
 +
</h6>
 
<p>
 
<p>
−
   Provided advice for design choices and taking criticism regarding said design choices.
+
   UCSC iGEM 2017 provided us with inspiration for the UCSC 2018 wiki and, more specifically, a layout for the Attributions page.
−
</p><br>
+
</p>
  
 +
<h6>
 +
  <b> Shayan Vahdani </b>
 +
</h6>
 +
<p>
 +
  Shayan provided feedback on our wiki design and offered alternatives to make our display better.
 +
</p>
  
−
<p class="p-title">
+
   </div>
−
   <b> UCSC iGEM 2017 </b> </SPAN>
+
−
</p>
+
−
<p>
+
−
  For providing us with inspiration for the UCSC 2018 wiki and, more specifically, a layout for the Attributions page.
+
−
</p><br>
+
 
</div>
 
</div>
−
</div>
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
  
  
Line 421: Line 350:
 
<!--      ************************** Human Practices Support *******************************      -->
 
<!--      ************************** Human Practices Support *******************************      -->
 
<!--      **********************************************************************************      -->
 
<!--      **********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="HPS">Human Practices Support </p>
+
    <h3 id="Attr_HPS"> Human Practices Support </h3>
−
</div>
+
−
</div>
+
  
 +
<h6>
 +
  <b> Sandra Dreisbach, </b>
 +
  <i>Professor, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Professor Dreisbach introduced our team to the IRB and prompted us to look into IRB exemption for our questionnaire.
 +
</p>
  
 +
<h6>
 +
  <b> Emily Fisch, </b>
 +
  <i>Research Compliance Analyst, Office of Research Compliance Administration, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Emily for assisted our team in gaining IRB exemption for the Human Practices Birth Control Questionnaire.
 +
</p>
  
 +
<h6>
 +
  <b> Hank Pellissier, </b>
 +
  <i>Director, Brighter Brains Institute</i>
 +
</h6>
 +
<p>
 +
  Pellissier provided our Human Practices team several women's groups to contact in Uganda.
 +
</p>
  
 +
<h6>
 +
  <b> Bri Quinn, </b>
 +
  <i>Compliance Assistant, Institutional Review Board, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Bri for encouraged our team to apply for IRB exemption rather than a full protocol review.
 +
</p>
  
−
 
−
 
−
 
−
<br>
 
−
<br>
 
−
  <div class="flex-row">
 
−
    <div class="content-divider">
 
−
      <img src="https://static.igem.org/mediawiki/2018/d/d9/T--UCSC--FlowerDivider.png" alt="divider image">
 
−
    </div>
 
 
   </div>
 
   </div>
−
<br>
+
</div>
−
<br>
+
−
<br>
+
−
 
+
  
  
Line 453: Line 394:
 
<!--      ******************************* Funding Support **********************************      -->
 
<!--      ******************************* Funding Support **********************************      -->
 
<!--      **********************************************************************************      -->
 
<!--      **********************************************************************************      -->
−
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="FndS"> Funding Support</p>
+
    <h3 id="Attr_FndS"> Funding Support </h3>
  
  
−
<p class="p-title">  
+
<h6>  
−
   <b> UCSC iGEM 2017 </b> </SPAN>  
+
   <b> UCSC iGEM 2017 </b>
 +
</h6>
 +
<p>
 +
  UCSC iGEM 2017 provided monetary support for our 2018 team.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> George Spix </b>
 +
</h6>
 
<p>
 
<p>
−
   Provide monetary support for our team.
+
   George Spix provided significant monetary support for the 2018 team and future iGEM teams.
−
</p><br>
+
</p>
  
 
</div>
 
</div>
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
 
  
  
Line 479: Line 421:
 
<!--      ****************************** Industry Support **********************************      -->
 
<!--      ****************************** Industry Support **********************************      -->
 
<!--      **********************************************************************************      -->
 
<!--      **********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="IndS">Industry Support</p>
+
    <h3 id="Attr_IndS">Industry Support</h3>
  
−
<p class="p-title">
+
<h6>
−
   <b> Fungal Genetics Stock Center, </b>  
+
   <b> Asana </b>  
 +
</h6>
 +
<p>
 +
  Asana provided/sponsored our team with free premium accounts to organize and communicate work ideas with each other.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> Fungal Genetics Stock Center </b>
 +
</h6>
 
<p>
 
<p>
−
   Provided the team with Yarrowia Lipolytica Strain.
+
   The FGSC provided our team with a <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> strain.
−
</p><br>
+
−
 
+
−
 
+
−
<p class="p-title">
+
−
  <b> Asana, </b>  
+
 
</p>
 
</p>
−
<p>
 
−
  Provided team with free premium accounts to organize and communicate work ideas with each other.
 
−
</p><br>
 
  
−
 
+
  </div>
−
 
+
−
</div>
+
 
</div>
 
</div>
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
  
  
Line 521: Line 447:
 
<!--      ***************************** Non Profit Partnerships ***************************      -->
 
<!--      ***************************** Non Profit Partnerships ***************************      -->
 
<!--      *********************************************************************************      -->
 
<!--      *********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="NPP"> Non Profit Partnerships </p>
+
    <h3 id="Attr_NPP"> Non-Profit Partnerships </h3>
−
</div>
+
−
</div>
+
  
 +
<h6>
 +
  <b> Hank Pellissier, </b>
 +
  <i>Director, Brighter Brains Institute</i>
 +
</h6>
 +
<p>
 +
  Pellissier provided our Human Practices team several women's groups to contact in Uganda.
 +
</p>
  
 +
<h6>
 +
  <b> Martyn Smith, </b>
 +
  <i>Managing Director, Family Planning 2020</i>
 +
</h6>
 +
<p>
 +
  Martyn provided us contacts to funding bodies that regulate drug production in several of our target countries. Martyn also passed on our team's message to his coworkers and partners, including the Bill and Melinda Gates Foundation.
 +
</p>
  
 +
<h6>
 +
  <b> John Townsend, </b>
 +
  <i>Director, County Strategy, Population Council</i>
 +
</h6>
 +
<p>
 +
  John discussed possible pathways to further develop our contraceptive outside of the iGEM competition.
 +
</p>
  
−
 
−
 
−
 
−
<br>
 
−
<br>
 
−
  <div class="flex-row">
 
−
    <div class="content-divider">
 
−
      <img src="https://static.igem.org/mediawiki/2018/d/d9/T--UCSC--FlowerDivider.png" alt="divider image">
 
−
    </div>
 
 
   </div>
 
   </div>
−
<br>
 
−
<br>
 
−
<br>
 
−
 
−
 
−
 
−
 
−
<!--      *********************************************************************************      -->
 
−
<!--      ************************** Difficult Technique Support **************************      -->
 
−
<!--      *********************************************************************************      -->
 
−
 
−
 
−
<div class="flex-row">
 
−
<div class="flex-col">
 
−
<p style="font-size:150% !important" class="week" id="DTS">Difficult Technique Support</p>
 
 
</div>
 
</div>
−
</div>
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
−
 
  
  
Line 573: Line 483:
 
<!--      ****************************** Presentation Support *****************************      -->
 
<!--      ****************************** Presentation Support *****************************      -->
 
<!--      *********************************************************************************      -->
 
<!--      *********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="PrsS">Presentation Support</p>
+
    <h3 id="Attr_PrsS">Presentation Support</h3>
−
</div>
+
−
</div>
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
−
 
+
  
 +
<h6>
 +
  <b> Zia Isola, </b>
 +
  <i>Director, Genomics Institute Office of Diversity Programs, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Zia helped us prepare our poster for the UCSC 9th Annual Physical and Biological Sciences Summer Research Symposium.
 +
</p>
  
 +
  </div>
 +
</div>
  
  
Line 594: Line 503:
 
<!--      ********************************** General Support ******************************      -->
 
<!--      ********************************** General Support ******************************      -->
 
<!--      *********************************************************************************      -->
 
<!--      *********************************************************************************      -->
−
<br>
 
−
<br>
 
  
 
<div class="flex-row">
 
<div class="flex-row">
−
<div class="flex-col">
+
  <div class="flex-col">
−
<p style="font-size:150% !important" class="week" id="GenS">General Support</p>
+
    <h3 id="Attr_GenS">General Support</h3>
  
−
 
+
<h6>
−
<p class="p-title">
+
 
   <b> Russell Evans, </b>  
 
   <b> Russell Evans, </b>  
 
   <i> Senior Development Engineer, University of California, Santa Cruz, Campus Facilities </i>
 
   <i> Senior Development Engineer, University of California, Santa Cruz, Campus Facilities </i>
 +
</h6>
 +
<p>
 +
  Russell provided the 2018 UCSC iGEM Team with separate dry lab research training and wet lab rooms. He also provided technical and equipment support.
 
</p>
 
</p>
 +
 +
<h6>
 +
  <b> Logan Mulroney, </b>
 +
  <i> PhD Student, University of California, Santa Cruz, Bioinformatics </i>
 +
</h6>
 
<p>
 
<p>
−
   Provided the <b> 2018 UCSC iGEM Team </b> with separate dry lab research and wet lab rooms. Provided technical and equipment support.
+
   Logan provided the 2018 UCSC iGEM Team with general advice on project design and lab techniques.
−
</p><br>
+
</p>
−
</div>
+
−
</div>
+
  
  
−
<br>
+
  </div>
−
 
+
</div>
  
 +
  <div class="flex-row">
 +
    <div class="content-divider">
 +
      <img src="https://static.igem.org/mediawiki/2018/d/d9/T--UCSC--FlowerDivider.png" alt="divider image">
 +
    </div>
 +
  </div>
  
−
    <a href="https://2017.igem.org/Team:UCSC">
+
  <a href="https://2017.igem.org/Team:UCSC">
−
      <img class="main_image" src="https://static.igem.org/mediawiki/2018/c/cd/T--UCSC--2017Team.jpg" alt="2017 Team Image" style="margin-bottom:-40px">
+
    <img class="main_image" src="https://static.igem.org/mediawiki/2018/c/cd/T--UCSC--2017Team.jpg" alt="2017 Team Image" style="margin-bottom:-40px">
−
    </a>
+
  </a>
  
 
<script language="JavaScript">
 
<script language="JavaScript">

Latest revision as of 02:47, 18 October 2018

Attributions

  • Attributions

  • The Project Idea
  • Official Team
  • Advisors
  • Research Support
  • Lab Support
  • Wiki Support
  • Human Practices Support
  • Funding Support
  • Industry Support
  • Non-Profit Partnerships
  • Presentation Support
  • General Support

    • Thank You

    The PoPPY team would like to thank everyone who made this project possible, especially those described below. For the past five years, undergraduates at UCSC have participated in iGEM. This year, the team is composed of students studying Bioengineering (Biomolecular), Bioinformatics, Bioengineering (Assistive Technology: Motor), Molecular, Cellular, and Developmental Biology, Marine Biology, and Cognitive Science: Artificial Intelligence and Human Computer Interaction. Our diverse group of individuals is crucial for the creative process behind using synthetic biology to help others. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2018 UCSC iGEM team is solving worldwide issues and developing methods to expand their research in the future.

    divider image

    The Project Idea

    After several journal club meetings, our team determined the PoPPY project was the most meaningful pursuit. The initial idea was proposed by captain Morgan Tardy and mentor McKenna Hicks.

    Official Team

    Thank you to all the 2018 UCSC iGEM Team members for their hard work and dedication to the PoPPY project's message. Check out our Meet the Team page to learn about each teammate.

    Advisors

    David Bernick, Assistant Adjunct Professor, University of California Santa Cruz, Department of Biomolecular Engineering

    Dr. Bernick for guided our team and offered valuable insight and advice through each stage of the project. He helped our team manage funding, finances and logistical tasks.


    McKenna Hicks, Master's Student, University of California Santa Cruz, Department of Biomolecular Engineering

    McKenna for provided advice on gene design and wet lab experiments.

    Todd Lowe, Professor, University of California Santa Cruz, Department of Biomolecular Engineering

    Todd Lowe acted as our assistant advisor and provided advice on coding for our website.

    Research Support

    Hal Alper, Professor, University of Texas at Austin, Department of Chemical Engineering

    Professor Alper provided recommendations for Tsynth27/Tsynth8 terminators and confirmed which promoters would be necessary for our project. Through a Materials Transfer Agreement, Alper sent us plasmids carrying terminators and fluorescent markers to test for gene activity.

    William Belden, Associate Professor, Rutgers University, Department of Animal Sciences

    Professor Belden provided valuable information on yeast-mediated cloning. Professor Belden inspired our parallel experiment design and even offered to help design our flanking oligos.

    Erin Bredeweg, Biosystems Dynamics & Simula Scientist, Pacific Northwest National Laboratory

    Erin Bredeweg assisted us in finding sequence information on our Yarrowia lipolytica strain.

    Tony Carr, Professor of Molecular Genetics, University of Sussex, Genome Damage and Stability, Genome Damage and Stability Centre

    Professor Carr answered our questions regarding the size of genomic insert, directionality of Lox sites, and cre-lox recombination.

    Catherine Duport, University of Avignon, Microbiotechnology, Ecotoxicology, and Bioremediation

    Catherine Duport helped clarify pieces of her 1998 experiment such as why certain promoters and genes were chosen. Duport also attempted to find us a Yarrowia lipolytica strain.

    Daniel Gallie, Professor, University of California Riverside, Biochemistry Department

    Professor Gallie sent us an electroporation protocol with mRNA for reference.

    Marc Gartenberg, Professor, Rutgers University, Department of Biochemistry and Molecular Biology

    Professor Gartenberg provided information on parts of pXRU3/L2 plasmid including HOp region, CEN/ARS regions, pRS marker, and how to insert Lox sites into these particular plasmids.

    Rohinton Kamakaka, Professor, University of California Santa Cruz, Department of Molecular Cellular and Developmental Biology

    Professor Kamakaka sent us protocols on yeast-mediated cloning. He also answered questions on growing and maintaining yeast and even provided us a Saccharomyces Cerevisiae strain for yeast-mediated cloning. Professor Kamakaka has also provided general support and advice for all our experiments.

    Oliver Konzock, PhD Student, Chalmers University, Life Science Engineering

    Oliver Konzock answered questions on how to grow yeast (and care for Yarrowia lipolytica). He also answered questions on how to successfully perform yeast-mediated cloning.

    Jah Nehlin, Copenhagen University Hospital Hvidovre

    Mehlin gave insight into how the GAL1 promoter works.

    Jean-Marc Nicaud, Directeur de Recherche INRA

    Jean-Marc Nicaud introduced us to the Golden Gate Assembly method. He also answered questions about building plasmids and showed us M1G1 wouldn't be an issue in terms of our promoter/terminator design.

    Saumya Ramarao, Senior Associate, Reproductive Health Supplies Coalition

    Ramarao provided information regarding birth control production and needs.

    Hans Ronne, Professor, Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology

    Professor Ronne attempted to determine how to stop the binding of MIG1 to GALI promoter.

    Cory Schwartz, PhD Student, University of California Riverside, Department of Civil and Environmental Engineering

    Schwartz provided pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.

    Wendy Sun, University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering

    Sun assisted Xu in proving that Yarrowia lipolytica could produce progesterone.

    Stefan Tippmann, Visiting Researcher, Chalmers University, Life Science Engineering

    Tippmann taught us about yeast in general and providing us with additional readings to learn about Yarrowia lipolytica.

    Adam Watson, Research Fellow, University of Sussex, Genome Damage and Stability

    Watson answered questions regarding the size of genomic inserts, directionality of Lox sites, and Cre-lox recombination.

    Ian Wheeldon, Associate Professor, University of California Riverside, Department of Chemical and Environmental Engineering

    Wheeldon provided the pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.

    Peng Xu, University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering

    Xu proved that Yarrowia lipolytica could produce progesterone despite its NHEJ preference. He showed that it is possible to use homologous recombination with a KU70 knockout strain.

    Lab Support

    Dr. Hugh Olsen, Lab Manager, University of California, Santa Cruz, Biomolecular Engineering Teaching Labs

    Dr. Olsen provided assistance with autoclave-training, general laboratory safety training, obtaining materials and maintaining lab equipment.

    Wiki Support

    Evan Pepper, Former Undergraduate Student, University of California Santa Cruz, Biomolecular Engineering

    Evan provided advice on design choices and website organization.

    UCSC iGEM 2017

    UCSC iGEM 2017 provided us with inspiration for the UCSC 2018 wiki and, more specifically, a layout for the Attributions page.

    Shayan Vahdani

    Shayan provided feedback on our wiki design and offered alternatives to make our display better.

    Human Practices Support

    Sandra Dreisbach, Professor, University of California Santa Cruz

    Professor Dreisbach introduced our team to the IRB and prompted us to look into IRB exemption for our questionnaire.

    Emily Fisch, Research Compliance Analyst, Office of Research Compliance Administration, University of California Santa Cruz

    Emily for assisted our team in gaining IRB exemption for the Human Practices Birth Control Questionnaire.

    Hank Pellissier, Director, Brighter Brains Institute

    Pellissier provided our Human Practices team several women's groups to contact in Uganda.

    Bri Quinn, Compliance Assistant, Institutional Review Board, University of California Santa Cruz

    Bri for encouraged our team to apply for IRB exemption rather than a full protocol review.

    Funding Support

    UCSC iGEM 2017

    UCSC iGEM 2017 provided monetary support for our 2018 team.

    George Spix

    George Spix provided significant monetary support for the 2018 team and future iGEM teams.

    Industry Support

    Asana

    Asana provided/sponsored our team with free premium accounts to organize and communicate work ideas with each other.

    Fungal Genetics Stock Center

    The FGSC provided our team with a Yarrowia lipolytica strain.

    Non-Profit Partnerships

    Hank Pellissier, Director, Brighter Brains Institute

    Pellissier provided our Human Practices team several women's groups to contact in Uganda.

    Martyn Smith, Managing Director, Family Planning 2020

    Martyn provided us contacts to funding bodies that regulate drug production in several of our target countries. Martyn also passed on our team's message to his coworkers and partners, including the Bill and Melinda Gates Foundation.

    John Townsend, Director, County Strategy, Population Council

    John discussed possible pathways to further develop our contraceptive outside of the iGEM competition.

    Presentation Support

    Zia Isola, Director, Genomics Institute Office of Diversity Programs, University of California Santa Cruz

    Zia helped us prepare our poster for the UCSC 9th Annual Physical and Biological Sciences Summer Research Symposium.

    General Support

    Russell Evans, Senior Development Engineer, University of California, Santa Cruz, Campus Facilities

    Russell provided the 2018 UCSC iGEM Team with separate dry lab research training and wet lab rooms. He also provided technical and equipment support.

    Logan Mulroney, PhD Student, University of California, Santa Cruz, Bioinformatics

    Logan provided the 2018 UCSC iGEM Team with general advice on project design and lab techniques.

    divider image
    2017 Team Image