Difference between revisions of "Team:UCSC/Attributions"

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  <div class="header-image" id="header_image_attributions" style="background-image: url(https://static.igem.org/mediawiki/2018/c/c7/T--UCSC--Collab_Background.png); background-position: 40% 60%">
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    <h1>
 +
      Attributions
 +
    </h1>
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  </div>
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  <!-- Include arrow to jump to top -->
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  <a href="#top_anchor" class="scroll-to-top"><img src="https://static.igem.org/mediawiki/2018/c/cd/T--UCSC--Arrow.png"></a>
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<!--      ************************************* Table of Contents ***************************************      -->
  
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<div class="column full_size judges-will-not-evaluate">
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<div class="float-toc">
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<h3>★  ALERT! </h3>
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  <div class="toc-container">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
+
  <li><h3 id="top_anchor"> Attributions </h3></li>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
  <li><a href="#Attr_Idea"> The Project Idea </a></li>
 +
  <li><a href="#Attr_Team"> Official Team </a></li>
 +
  <li><a href="#Attr_Advi"> Advisors </a></li>
 +
  <li><a href="#Attr_ResS"> Research Support </a></li>
 +
  <li><a href="#Attr_LabS"> Lab Support </a></li>
 +
  <li><a href="#Attr_WikS"> Wiki Support </a></li>
 +
  <li><a href="#Attr_HPS"> Human Practices Support </a></li>
 +
  <li><a href="#Attr_FndS"> Funding Support </a></li>
 +
  <li><a href="#Attr_IndS"> Industry Support </a></li>
 +
  <li><a href="#Attr_NPP"> Non-Profit Partnerships </a></li>
 +
  <li><a href="#Attr_PrsS"> Presentation Support </a></li>
 +
  <li><a href="#Attr_GenS"> General Support </a></li>
 +
  </div>
 
</div>
 
</div>
  
 +
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<div class="clear"></div>
 
  
 +
  <div class="flex-row">
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    <div class="flex-col">
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      <h2> Thank You </h2>
 +
      <p><b>The PoPPY team would like to thank everyone who made this project possible</b>, especially those described below. For the past five years, undergraduates at UCSC have participated in iGEM. This year, the team is composed of students studying Bioengineering (Biomolecular), Bioinformatics, Bioengineering (Assistive Technology: Motor), Molecular, Cellular, and Developmental Biology, Marine Biology, and Cognitive Science: Artificial Intelligence and Human Computer Interaction. Our diverse group of individuals is crucial for the creative process behind using synthetic biology to help others. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2018 UCSC iGEM team is solving worldwide issues and developing methods to expand their research in the future.</p>
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 +
  </div>
  
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  <div class="flex-row">
 +
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      <img src="https://static.igem.org/mediawiki/2018/d/d9/T--UCSC--FlowerDivider.png" alt="divider image">
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    </div>
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  </div>
  
  
  
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<div class="column full_size">
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<h1>Attributions</h1>
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<!--      *********************************** Project **************************************      -->
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<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
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<!--      **********************************************************************************      -->
 +
 
 +
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_Idea"> The Project Idea </h3>
 +
    <p> After several journal club meetings, our team determined the PoPPY project was the most meaningful pursuit. The initial idea was proposed by captain <b><u><a href="https://2018.igem.org/Team:UCSC/Team#morgan" style="text-decoration: none; color: black;">Morgan Tardy</a></u></b> and mentor <b><u><a href="https://2018.igem.org/Team:UCSC/Team#mckenna" style="text-decoration: none; color: black;">McKenna Hicks</a></u></b>.</p>
 +
  </div>
 +
</div>
 +
 
 +
 
 +
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 +
<!--      *********************************** Students *************************************      -->
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 +
 
 +
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_Team">Official Team</h3>
 +
Thank you to all the 2018 UCSC iGEM Team members for their hard work and dedication to the PoPPY project's message. Check out our <b><u><a href="https://2018.igem.org/Team:UCSC/Team" style="text-decoration: none; color: black;">Meet the Team page</a></u></b> to learn about each teammate.
 +
  </div>
 +
</div>
 +
 
 +
 
 +
<!--      **********************************************************************************      -->
 +
<!--      *********************************** Advisors *************************************      -->
 +
<!--      **********************************************************************************      -->
 +
 
 +
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_Advi">Advisors</h3>
 +
    <h6><b> David Bernick, </b>
 +
        <i> Assistant Adjunct Professor, University of California Santa Cruz, Department of Biomolecular Engineering </i></h6>
 +
    <p>Dr. Bernick for guided our team and offered valuable insight and advice through each stage of the project. He helped our team manage funding, finances and logistical tasks.</p>
 +
    <br>
 +
 
 +
    <h6><b> McKenna Hicks, </b>
 +
        <i> Master's Student, University of California Santa Cruz, Department of Biomolecular Engineering </i></h6>
 +
    <p>McKenna for provided advice on gene design and wet lab experiments.</p>
 +
 
 +
<h6><b> Todd Lowe, </b>
 +
        <i> Professor, University of California Santa Cruz, Department of Biomolecular Engineering </i></h6>
 +
    <p>Todd Lowe acted as our assistant advisor and provided advice on coding for our website.</p>
 +
 
 +
  </div>
 +
</div>
 +
 
 +
 
 +
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 +
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 +
<!--      **********************************************************************************      -->
 +
 
 +
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_ResS">Research Support</h3>
 +
<h6>
 +
  <b> Hal Alper, </b>
 +
  <i> Professor, University of Texas at Austin, Department of Chemical Engineering </i> 
 +
</h6>
 +
<p>
 +
  Professor Alper provided recommendations for Tsynth27/Tsynth8 terminators and confirmed which promoters would be necessary for our project. Through a Materials Transfer Agreement, Alper sent us plasmids carrying terminators and fluorescent markers to test for gene activity.
 +
</p>
 +
 
 +
<h6>
 +
  <b> William Belden, </b>
 +
  <i> Associate Professor, Rutgers University, Department of Animal Sciences </i> 
 +
</h6>
 +
<p>
 +
  Professor Belden provided valuable information on yeast-mediated cloning. Professor Belden inspired our parallel experiment design and even offered to help design our flanking oligos.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Erin Bredeweg, </b>
 +
  <i> Biosystems Dynamics & Simula Scientist, Pacific Northwest National Laboratory </i>
 +
</h6>
 +
<p>
 +
  Erin Bredeweg assisted us in finding sequence information on our <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> strain.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Tony Carr, </b>
 +
  <i> Professor of Molecular Genetics, University of Sussex, Genome Damage and Stability, Genome Damage and Stability Centre </i>
 +
</h6>
 +
<p>
 +
  Professor Carr answered our questions regarding the size of genomic insert, directionality of Lox sites, and cre-lox recombination.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Catherine Duport, </b>
 +
  <i> University of Avignon, Microbiotechnology, Ecotoxicology, and Bioremediation </i>
 +
</h6>
 +
<p>
 +
  Catherine Duport helped clarify pieces of her 1998 experiment such as why certain promoters and genes were chosen. Duport also attempted to find us a <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> strain.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Daniel Gallie, </b>
 +
  <i> Professor, University of California Riverside, Biochemistry Department </i> 
 +
</h6>
 +
<p>
 +
  Professor Gallie sent us an electroporation protocol with mRNA for reference.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Marc Gartenberg, </b>
 +
  <i> Professor, Rutgers University, Department of Biochemistry and Molecular Biology </i> 
 +
</h6>
 +
<p>
 +
  Professor Gartenberg provided information on parts of pXRU3/L2 plasmid including HOp region, CEN/ARS regions, pRS marker, and how to insert Lox sites into these particular plasmids.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Rohinton Kamakaka, </b>
 +
  <i> Professor, University of California Santa Cruz, Department of Molecular Cellular and Developmental Biology </i> 
 +
</h6>
 +
<p>
 +
  Professor Kamakaka sent us protocols on yeast-mediated cloning. He also answered questions on growing and maintaining yeast and even provided us a <i style="font-size:100% !important">Saccharomyces Cerevisiae</i> strain for yeast-mediated cloning. Professor Kamakaka has also provided general support and advice for all our experiments.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Oliver Konzock, </b>
 +
  <i> PhD Student, Chalmers University, Life Science Engineering </i>
 +
</h6>
 +
<p>
 +
  Oliver Konzock answered questions on how to grow yeast (and care for <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i>). He also answered questions on how to successfully perform yeast-mediated cloning.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Jah Nehlin, </b>
 +
  <i> Copenhagen University Hospital Hvidovre </i> 
 +
</h6>
 +
<p>
 +
  Mehlin gave insight into how the GAL1 promoter works.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Jean-Marc Nicaud, </b>
 +
  <i> Directeur de Recherche INRA </i>
 +
</h6>
 +
<p>
 +
  Jean-Marc Nicaud introduced us to the Golden Gate Assembly method. He also answered questions about building plasmids and showed us M1G1 wouldn't be an issue in terms of our promoter/terminator design.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Saumya Ramarao, </b>
 +
  <i> Senior Associate, Reproductive Health Supplies Coalition </i> 
 +
</h6>
 +
<p>
 +
  Ramarao provided information regarding birth control production and needs.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Hans Ronne, </b>
 +
  <i> Professor, Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology </i> 
 +
</h6>
 +
<p>
 +
  Professor Ronne attempted to determine how to stop the binding of MIG1 to GALI promoter.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Cory Schwartz, </b>
 +
  <i> PhD Student, University of California Riverside, Department of Civil and Environmental Engineering </i> 
 +
</h6>
 +
<p>
 +
  Schwartz provided pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Wendy Sun, </b>
 +
  <i> University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering </i> 
 +
</h6>
 +
<p>
 +
  Sun assisted Xu in proving that <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> could produce progesterone.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Stefan Tippmann, </b>
 +
  <i> Visiting Researcher, Chalmers University, Life Science Engineering </i>
 +
</h6>
 +
<p>
 +
Tippmann taught us about yeast in general and providing us with additional readings to learn about <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i>.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Adam Watson, </b>
 +
  <i> Research Fellow, University of Sussex, Genome Damage and Stability </i> 
 +
</h6>
 +
<p>
 +
  Watson answered questions regarding the size of genomic inserts, directionality of Lox sites, and Cre-lox recombination.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Ian Wheeldon, </b>
 +
  <i> Associate Professor, University of California Riverside, Department of Chemical and Environmental Engineering </i> 
 +
</h6>
 +
<p>
 +
  Wheeldon provided the pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Peng Xu, </b>
 +
  <i> University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering </i> 
 +
</h6>
 +
<p>
 +
  Xu proved that <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> could produce progesterone despite its NHEJ preference. He showed that it is possible to use homologous recombination with a KU70 knockout strain.
 
</p>
 
</p>
  
−
<h3> Bronze Medal Criterion #3</h3>
+
  </div>
−
<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
+
−
<br><br>
+
−
Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
+
 
</div>
 
</div>
  
  
−
<div class="clear extra_space"></div>
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<!--      ********************************* Lab Support ************************************      -->
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 +
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_LabS"> Lab Support </h3>
  
 +
<h6>
 +
  <b> Dr. Hugh Olsen, </b>
 +
  <i> Lab Manager, University of California, Santa Cruz, Biomolecular Engineering Teaching Labs </i>
 +
</h6>
 +
<p>
 +
  Dr. Olsen provided assistance with autoclave-training, general laboratory safety training, obtaining materials and maintaining lab equipment.
 +
</p>
  
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<div class="column third_size">
+
  </div>
−
<h3> What should this page contain?</h3>
+
</div>
  
−
<ul>
+
 
−
<li>Clearly state what the team accomplished</li>
+
<!--      *********************************************************************************      -->
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<li>General Support</li>
+
<!--      ********************************** Wiki Support *********************************      -->
−
<li>Project support and advice</li>
+
<!--      *********************************************************************************      -->
−
<li>Fundraising help and advice</li>
+
 
−
<li>Lab support</li>
+
<div class="flex-row">
−
<li>Difficult technique support</li>
+
  <div class="flex-col">
−
<li>Project advisor support</li>
+
    <h3 id="Attr_WikS">Wiki Support</h3>
−
<li>Wiki support</li>
+
 
−
<li>Presentation coaching</li>
+
<h6>
−
<li>Human Practices support</li>
+
  <b> Evan Pepper, </b>  
−
<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
+
  <i> Former Undergraduate Student, University of California Santa Cruz, Biomolecular Engineering </i>
−
</ul>
+
</h6>
 +
<p>
 +
  Evan provided advice on design choices and website organization.
 +
</p>
 +
 
 +
<h6>
 +
  <b> UCSC iGEM 2017 </b>
 +
</h6>
 +
<p>
 +
  UCSC iGEM 2017 provided us with inspiration for the UCSC 2018 wiki and, more specifically, a layout for the Attributions page.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Shayan Vahdani </b>
 +
</h6>
 +
<p>
 +
  Shayan provided feedback on our wiki design and offered alternatives to make our display better.
 +
</p>
 +
 
 +
  </div>
 
</div>
 
</div>
  
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<div class="column third_size">
+
 
−
<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
+
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−
<ul>
+
<!--      ************************** Human Practices Support *******************************      -->
−
<li>Does your institution teach an iGEM or synthetic biology course?</li>
+
<!--      **********************************************************************************      -->
−
<li>When did you start this course?</li>
+
 
−
<li>Are the syllabus and course materials freely available online?</li>
+
<div class="flex-row">
−
<li>When did you start your brainstorming?</li>
+
  <div class="flex-col">
−
<li>When did you start in the lab?</li>
+
    <h3 id="Attr_HPS"> Human Practices Support </h3>
−
<li>When did you start working on  your project?</li>
+
 
−
</ul>
+
<h6>
 +
  <b> Sandra Dreisbach, </b>
 +
  <i>Professor, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Professor Dreisbach introduced our team to the IRB and prompted us to look into IRB exemption for our questionnaire.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Emily Fisch, </b>  
 +
  <i>Research Compliance Analyst, Office of Research Compliance Administration, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Emily for assisted our team in gaining IRB exemption for the Human Practices Birth Control Questionnaire.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Hank Pellissier, </b>  
 +
  <i>Director, Brighter Brains Institute</i>
 +
</h6>
 +
<p>
 +
  Pellissier provided our Human Practices team several women's groups to contact in Uganda.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Bri Quinn, </b>  
 +
  <i>Compliance Assistant, Institutional Review Board, University of California Santa Cruz</i>
 +
</h6>
 +
<p>
 +
  Bri for encouraged our team to apply for IRB exemption rather than a full protocol review.
 +
</p>
 +
 
 +
  </div>
 
</div>
 
</div>
  
  
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<div class="column third_size">
+
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<div class="highlight decoration_A_full">
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<!--      ******************************* Funding Support **********************************      -->
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 +
 
 +
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_FndS"> Funding Support </h3>
 +
 
 +
 
 +
<h6>
 +
  <b> UCSC iGEM 2017 </b>
 +
</h6>
 +
<p>
 +
  UCSC iGEM 2017 provided monetary support for our 2018 team.
 +
</p>
 +
 
 +
<h6>
 +
  <b> George Spix </b>
 +
</h6>
 +
<p>
 +
  George Spix provided significant monetary support for the 2018 team and future iGEM teams.
 +
</p>
  
−
<h3>Inspiration</h3>
 
−
<p>Take a look at what other teams have done:</p>
 
−
<ul>
 
−
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
 
−
<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
 
−
</ul>
 
 
</div>
 
</div>
 
</div>
 
</div>
  
  
 +
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 +
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 +
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−
<div class="clear extra_space"></div>
+
<div class="flex-row">
 +
  <div class="flex-col">
 +
    <h3 id="Attr_IndS">Industry Support</h3>
  
−
<div class="column two_thirds_size">
+
<h6>
−
<h3> Why is this page needed? </h3>
+
  <b> Asana </b>  
−
<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
+
</h6>
 
<p>
 
<p>
−
For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
+
  Asana provided/sponsored our team with free premium accounts to organize and communicate work ideas with each other.
 +
</p>
 +
 
 +
<h6>
 +
  <b> Fungal Genetics Stock Center </b>
 +
</h6>
 +
<p>
 +
  The FGSC provided our team with a <i><span style="font-size:125% !important">Yarrowia lipolytica</span></i> strain.
 +
</p>
 +
 
 +
  </div>
 
</div>
 
</div>
  
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<div class="column third_size">
+
 
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<h3> Can we base our project on a previous one? </h3>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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  <b> Hank Pellissier, </b>
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  <i>Director, Brighter Brains Institute</i>
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</h6>
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<p>
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  Pellissier provided our Human Practices team several women's groups to contact in Uganda.
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</p>
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<h6>
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  <b> Martyn Smith, </b>  
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  <i>Managing Director, Family Planning 2020</i>
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</h6>
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<p>
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  Martyn provided us contacts to funding bodies that regulate drug production in several of our target countries. Martyn also passed on our team's message to his coworkers and partners, including the Bill and Melinda Gates Foundation.
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</p>
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<h6>
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  <b> John Townsend, </b>
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  <i>Director, County Strategy, Population Council</i>
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</h6>
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<p>
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  John discussed possible pathways to further develop our contraceptive outside of the iGEM competition.
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<h6>
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  <b> Zia Isola, </b>
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  <i>Director, Genomics Institute Office of Diversity Programs, University of California Santa Cruz</i>
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</h6>
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<p>
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  Zia helped us prepare our poster for the UCSC 9th Annual Physical and Biological Sciences Summer Research Symposium.
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  <b> Russell Evans, </b>
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  <i> Senior Development Engineer, University of California, Santa Cruz, Campus Facilities </i>
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</h6>
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<p>
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  Russell provided the 2018 UCSC iGEM Team with separate dry lab research training and wet lab rooms. He also provided technical and equipment support.
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</p>
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<h6>
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  <b> Logan Mulroney, </b>
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  <i> PhD Student, University of California, Santa Cruz, Bioinformatics </i>
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</h6>
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<p>
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  Logan provided the 2018 UCSC iGEM Team with general advice on project design and lab techniques.
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{{:Team:UCSC/Footer}}

Latest revision as of 02:47, 18 October 2018

Attributions

  • Attributions

  • The Project Idea
  • Official Team
  • Advisors
  • Research Support
  • Lab Support
  • Wiki Support
  • Human Practices Support
  • Funding Support
  • Industry Support
  • Non-Profit Partnerships
  • Presentation Support
  • General Support

    • Thank You

    The PoPPY team would like to thank everyone who made this project possible, especially those described below. For the past five years, undergraduates at UCSC have participated in iGEM. This year, the team is composed of students studying Bioengineering (Biomolecular), Bioinformatics, Bioengineering (Assistive Technology: Motor), Molecular, Cellular, and Developmental Biology, Marine Biology, and Cognitive Science: Artificial Intelligence and Human Computer Interaction. Our diverse group of individuals is crucial for the creative process behind using synthetic biology to help others. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2018 UCSC iGEM team is solving worldwide issues and developing methods to expand their research in the future.

    divider image

    The Project Idea

    After several journal club meetings, our team determined the PoPPY project was the most meaningful pursuit. The initial idea was proposed by captain Morgan Tardy and mentor McKenna Hicks.

    Official Team

    Thank you to all the 2018 UCSC iGEM Team members for their hard work and dedication to the PoPPY project's message. Check out our Meet the Team page to learn about each teammate.

    Advisors

    David Bernick, Assistant Adjunct Professor, University of California Santa Cruz, Department of Biomolecular Engineering

    Dr. Bernick for guided our team and offered valuable insight and advice through each stage of the project. He helped our team manage funding, finances and logistical tasks.


    McKenna Hicks, Master's Student, University of California Santa Cruz, Department of Biomolecular Engineering

    McKenna for provided advice on gene design and wet lab experiments.

    Todd Lowe, Professor, University of California Santa Cruz, Department of Biomolecular Engineering

    Todd Lowe acted as our assistant advisor and provided advice on coding for our website.

    Research Support

    Hal Alper, Professor, University of Texas at Austin, Department of Chemical Engineering

    Professor Alper provided recommendations for Tsynth27/Tsynth8 terminators and confirmed which promoters would be necessary for our project. Through a Materials Transfer Agreement, Alper sent us plasmids carrying terminators and fluorescent markers to test for gene activity.

    William Belden, Associate Professor, Rutgers University, Department of Animal Sciences

    Professor Belden provided valuable information on yeast-mediated cloning. Professor Belden inspired our parallel experiment design and even offered to help design our flanking oligos.

    Erin Bredeweg, Biosystems Dynamics & Simula Scientist, Pacific Northwest National Laboratory

    Erin Bredeweg assisted us in finding sequence information on our Yarrowia lipolytica strain.

    Tony Carr, Professor of Molecular Genetics, University of Sussex, Genome Damage and Stability, Genome Damage and Stability Centre

    Professor Carr answered our questions regarding the size of genomic insert, directionality of Lox sites, and cre-lox recombination.

    Catherine Duport, University of Avignon, Microbiotechnology, Ecotoxicology, and Bioremediation

    Catherine Duport helped clarify pieces of her 1998 experiment such as why certain promoters and genes were chosen. Duport also attempted to find us a Yarrowia lipolytica strain.

    Daniel Gallie, Professor, University of California Riverside, Biochemistry Department

    Professor Gallie sent us an electroporation protocol with mRNA for reference.

    Marc Gartenberg, Professor, Rutgers University, Department of Biochemistry and Molecular Biology

    Professor Gartenberg provided information on parts of pXRU3/L2 plasmid including HOp region, CEN/ARS regions, pRS marker, and how to insert Lox sites into these particular plasmids.

    Rohinton Kamakaka, Professor, University of California Santa Cruz, Department of Molecular Cellular and Developmental Biology

    Professor Kamakaka sent us protocols on yeast-mediated cloning. He also answered questions on growing and maintaining yeast and even provided us a Saccharomyces Cerevisiae strain for yeast-mediated cloning. Professor Kamakaka has also provided general support and advice for all our experiments.

    Oliver Konzock, PhD Student, Chalmers University, Life Science Engineering

    Oliver Konzock answered questions on how to grow yeast (and care for Yarrowia lipolytica). He also answered questions on how to successfully perform yeast-mediated cloning.

    Jah Nehlin, Copenhagen University Hospital Hvidovre

    Mehlin gave insight into how the GAL1 promoter works.

    Jean-Marc Nicaud, Directeur de Recherche INRA

    Jean-Marc Nicaud introduced us to the Golden Gate Assembly method. He also answered questions about building plasmids and showed us M1G1 wouldn't be an issue in terms of our promoter/terminator design.

    Saumya Ramarao, Senior Associate, Reproductive Health Supplies Coalition

    Ramarao provided information regarding birth control production and needs.

    Hans Ronne, Professor, Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology

    Professor Ronne attempted to determine how to stop the binding of MIG1 to GALI promoter.

    Cory Schwartz, PhD Student, University of California Riverside, Department of Civil and Environmental Engineering

    Schwartz provided pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.

    Wendy Sun, University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering

    Sun assisted Xu in proving that Yarrowia lipolytica could produce progesterone.

    Stefan Tippmann, Visiting Researcher, Chalmers University, Life Science Engineering

    Tippmann taught us about yeast in general and providing us with additional readings to learn about Yarrowia lipolytica.

    Adam Watson, Research Fellow, University of Sussex, Genome Damage and Stability

    Watson answered questions regarding the size of genomic inserts, directionality of Lox sites, and Cre-lox recombination.

    Ian Wheeldon, Associate Professor, University of California Riverside, Department of Chemical and Environmental Engineering

    Wheeldon provided the pHR_AO8_hrGFP plasmid sequence that includes homologous arm regions.

    Peng Xu, University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering

    Xu proved that Yarrowia lipolytica could produce progesterone despite its NHEJ preference. He showed that it is possible to use homologous recombination with a KU70 knockout strain.

    Lab Support

    Dr. Hugh Olsen, Lab Manager, University of California, Santa Cruz, Biomolecular Engineering Teaching Labs

    Dr. Olsen provided assistance with autoclave-training, general laboratory safety training, obtaining materials and maintaining lab equipment.

    Wiki Support

    Evan Pepper, Former Undergraduate Student, University of California Santa Cruz, Biomolecular Engineering

    Evan provided advice on design choices and website organization.

    UCSC iGEM 2017

    UCSC iGEM 2017 provided us with inspiration for the UCSC 2018 wiki and, more specifically, a layout for the Attributions page.

    Shayan Vahdani

    Shayan provided feedback on our wiki design and offered alternatives to make our display better.

    Human Practices Support

    Sandra Dreisbach, Professor, University of California Santa Cruz

    Professor Dreisbach introduced our team to the IRB and prompted us to look into IRB exemption for our questionnaire.

    Emily Fisch, Research Compliance Analyst, Office of Research Compliance Administration, University of California Santa Cruz

    Emily for assisted our team in gaining IRB exemption for the Human Practices Birth Control Questionnaire.

    Hank Pellissier, Director, Brighter Brains Institute

    Pellissier provided our Human Practices team several women's groups to contact in Uganda.

    Bri Quinn, Compliance Assistant, Institutional Review Board, University of California Santa Cruz

    Bri for encouraged our team to apply for IRB exemption rather than a full protocol review.

    Funding Support

    UCSC iGEM 2017

    UCSC iGEM 2017 provided monetary support for our 2018 team.

    George Spix

    George Spix provided significant monetary support for the 2018 team and future iGEM teams.

    Industry Support

    Asana

    Asana provided/sponsored our team with free premium accounts to organize and communicate work ideas with each other.

    Fungal Genetics Stock Center

    The FGSC provided our team with a Yarrowia lipolytica strain.

    Non-Profit Partnerships

    Hank Pellissier, Director, Brighter Brains Institute

    Pellissier provided our Human Practices team several women's groups to contact in Uganda.

    Martyn Smith, Managing Director, Family Planning 2020

    Martyn provided us contacts to funding bodies that regulate drug production in several of our target countries. Martyn also passed on our team's message to his coworkers and partners, including the Bill and Melinda Gates Foundation.

    John Townsend, Director, County Strategy, Population Council

    John discussed possible pathways to further develop our contraceptive outside of the iGEM competition.

    Presentation Support

    Zia Isola, Director, Genomics Institute Office of Diversity Programs, University of California Santa Cruz

    Zia helped us prepare our poster for the UCSC 9th Annual Physical and Biological Sciences Summer Research Symposium.

    General Support

    Russell Evans, Senior Development Engineer, University of California, Santa Cruz, Campus Facilities

    Russell provided the 2018 UCSC iGEM Team with separate dry lab research training and wet lab rooms. He also provided technical and equipment support.

    Logan Mulroney, PhD Student, University of California, Santa Cruz, Bioinformatics

    Logan provided the 2018 UCSC iGEM Team with general advice on project design and lab techniques.

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    2017 Team Image