Difference between revisions of "Team:CSU CHINA/Safety"

 
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{{CSU_CHINA}}
 
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<h1> Safety </h1>
 
<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<div class="block main-text main-text" >
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h class="highlight-title dark-blue"></br></br>General</h>
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<p>In Central South University, our school issues the laws of laboratory safety rules governing the biosafety, which follows the rules of Biological safety management regulations for pathogenic microorganisms Laboratory issued by China State Council.
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</p>
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<br/>
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<center>
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<img width="600" depth="300" src="https://static.igem.org/mediawiki/2018/9/95/T--CSU_CHINA--Safe1.png" />
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</center>
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<br/>
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<h class="highlight-title dark-blue"></br></br>Biosafety Training</h>
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<p>
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All the team members followed a lab safety training and signed relevant safety documents prior to entering the lab at Central South University. A thorough and comprehensive lab safety training was provided by the biosafety offices of the universities and the departmental specialists as the flow chart below. And the biosafety training also has a Internet-based test and values 1 academic point for bachelor students.
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</p>
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<center>
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<img width="600" depth="300" src="https://static.igem.org/mediawiki/2018/c/ce/T--CSU_CHINA--Safe5.jpg" />
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</center>
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<h class="highlight-title dark-blue"></br></br>Biosafety rules and documents</h>
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<p>
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Our lab follow China National Institute of Pathogenic Microorganisms Biosafety Management and Central South University Laboratory Safety Rules as these aspects below.
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<br/>
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Lab access and rules (those 3 rules below are specially supposed to remember for daily lab life)<br/>
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1.Eating and drinking are not allowed inside the working area.<br/>
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2.Anyone who is participating in the experiment is supposed to wear lab coats and protective goggles.<br/>
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3.You are not allowed to wear protective goggles to touch any resting area or somewhere outside the lab.<br/>
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Correct disposal of biological wastes and lab consumables<br/>
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Differences between biosafety levels<br/>
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Biosafety equipment and chemical safety(such as biosafety cabinets and chemical fume hood) <br/>
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Fire , water and electrical safety(We have fixed using time of large-scale scientific instruments and for sterilization)<br/>
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Disinfection and sterilization<br/>
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Transport rules<br/>
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Emergency procedures<br/>
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Responsible individuals (such as lab or departmental specialist or institutional biosafety officer)<br/>
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Good microbial technique (such as lab practices)<br/>
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</p>
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<h class="highlight-title dark-blue"></br></br>Lab Biosafety Level</h>
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<p>
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The iGEM CSU_CHINA team of 2018 were working mainly in the BSL-1 lab at the Department of Life Science, Central South University for our project.<br/>
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</p>
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<h class="highlight-title dark-blue"></br></br>Work areas</h>
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<p>
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Which work areas do you use to handle biological materials?<br/><br/>
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Open bench
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</p>
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<center>
 
<img width="600" depth="300" src="https://static.igem.org/mediawiki/2018/9/94/T--CSU_CHINA--labimage.jpeg ">
 
<img width="600" depth="300" src="https://static.igem.org/mediawiki/2018/9/94/T--CSU_CHINA--labimage.jpeg ">
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<br/>
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<p>
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Biosafety cabinet
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</p>
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<center>
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<img width="600" depth="300" src="https://static.igem.org/mediawiki/2018/5/55/T--CSU_CHINA--Safe3.png" />
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</center>
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<br/>
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<p>
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Chemical fume hood
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</p>
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<center>
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<img width="600" depth="300" src="https://static.igem.org/mediawiki/2018/f/fb/T--CSU_CHINA--Safe4.jpg" />
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</center>
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<br/>
  
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<h class="highlight-title dark-blue"></br></br>Personal protective equipment</h>
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<h3>Safe Project Design</h3>
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<p>
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Team members were equipped with lab coats, protective goggles, and disposable nitrile gloves. No other protective equipment was necessary according to the lab biosafety guidelines.<br/>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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</p>
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<h class="highlight-title dark-blue"></br></br>Chassis</h>
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<ul>
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<p>
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<li>Choosing a non-pathogenic chassis</li>
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We have worked with thecstrain of the biosafety level 1 approved bacterium E. coli  DH5alpha.<br/>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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</p>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<h class="highlight-title dark-blue"></br></br>Specific risks</h>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<p>
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</ul>
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Our project does not include gene drives, nor have we worked with animals. Despite of the standard antibiotics used in laboratory facilities (Ampicillin and Chloramphenicol), we have neither applied nor spread antimicrobial resistance factors. All species names (including strains) have been listed in the safety form.<br/>
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<br/>
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<a href="https://2018.igem.org/File:T--CSU_CHINA--Safety2018_Spreadsheet.xls">
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Click for Safety Form
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</a>
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<br/>
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</p>
  
 
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<h3>Safe Lab Work</h3>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h3>Safe Shipment</h3>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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</html>
 
</html>
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{{CSU_CHINA/Footer}}

Latest revision as of 03:00, 18 October 2018


Safety



General

In Central South University, our school issues the laws of laboratory safety rules governing the biosafety, which follows the rules of Biological safety management regulations for pathogenic microorganisms Laboratory issued by China State Council.





Biosafety Training

All the team members followed a lab safety training and signed relevant safety documents prior to entering the lab at Central South University. A thorough and comprehensive lab safety training was provided by the biosafety offices of the universities and the departmental specialists as the flow chart below. And the biosafety training also has a Internet-based test and values 1 academic point for bachelor students.



Biosafety rules and documents

Our lab follow China National Institute of Pathogenic Microorganisms Biosafety Management and Central South University Laboratory Safety Rules as these aspects below.
Lab access and rules (those 3 rules below are specially supposed to remember for daily lab life)
1.Eating and drinking are not allowed inside the working area.
2.Anyone who is participating in the experiment is supposed to wear lab coats and protective goggles.
3.You are not allowed to wear protective goggles to touch any resting area or somewhere outside the lab.
Correct disposal of biological wastes and lab consumables
Differences between biosafety levels
Biosafety equipment and chemical safety(such as biosafety cabinets and chemical fume hood)
Fire , water and electrical safety(We have fixed using time of large-scale scientific instruments and for sterilization)
Disinfection and sterilization
Transport rules
Emergency procedures
Responsible individuals (such as lab or departmental specialist or institutional biosafety officer)
Good microbial technique (such as lab practices)



Lab Biosafety Level

The iGEM CSU_CHINA team of 2018 were working mainly in the BSL-1 lab at the Department of Life Science, Central South University for our project.



Work areas

Which work areas do you use to handle biological materials?

Open bench


Biosafety cabinet


Chemical fume hood




Personal protective equipment

Team members were equipped with lab coats, protective goggles, and disposable nitrile gloves. No other protective equipment was necessary according to the lab biosafety guidelines.



Chassis

We have worked with thecstrain of the biosafety level 1 approved bacterium E. coli  DH5alpha.



Specific risks

Our project does not include gene drives, nor have we worked with animals. Despite of the standard antibiotics used in laboratory facilities (Ampicillin and Chloramphenicol), we have neither applied nor spread antimicrobial resistance factors. All species names (including strains) have been listed in the safety form.

Click for Safety Form