Difference between revisions of "Team:Nanjing-China/Design"

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<title>Nanjing-China2018</title>
 
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       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li></ul></div>
 
       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li></ul></div>
 
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     <li><a href="#cds"><font size="-1">CdS semiconductor</font></a></li>
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     <li><a href="#cds">CdS</a></li>
 
   <li><a href="#nitrogen"><font size="-1">Nitrogen fixation</font></a></li>
 
   <li><a href="#nitrogen"><font size="-1">Nitrogen fixation</font></a></li>
 
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<div id="for_judge" align="center"><div class="i"><ul><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges"><strong>For_judges</strong></a></ul></div></div>
 
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     <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a>
 
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    <div class="word" id="overview">
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<h2>Overview</h2>
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      <p>Our design is composed of three parts: biosynthesis of CdS semiconductor, light-driven nitrogen fixation and a light-driven biohybrid reaction device. This system is the expansion of our previous project of hydrogen production (Nanjing-China 2016), and it proves that surface display mechanism is capable of being expanded to a general principle for light-driven biohybrid reactions.</p>
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<h2>Biosynthesis of CdS semiconductor</h2>
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<h2>Biosynthesis of CdS semiconductor on cell surface</h2>
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<p>To construct our light-driven system, we first induce the precipitation of CdS semiconductor on the cell membrane. Two plasmids encoding the surface display protein OmpA-PbrR and the nitrogenase are co-transferred into <em>E. coli</em> strain. After Cd2+ is added into the media, the ions specifically bind to PbrR leading to aggregation of Cd2+ ions. At last when S2- ions are added into the media, <em>E. coli</em> cells for
+
<p align="left">To construct our light-driven system, we induce the in situ synthesis of CdS semiconductor on the <em>E. coli</em> cell surface. One key element of our system is fused protein OmpA-PbrR. OmpA (Outer membrane  protein A) fixes the protein complex on outer cell membrane while PbrR (lead-specific binding protein) adsorbs Cd<sup>2+</sup> in the environment and further forms CdS  semiconductor on cell surface.</p>
 +
<p> After Cd<sup>2+</sup> ions are added into the culture, the ions specifically bind to PbrR protein contributing to the  aggregation of Cd<sup>2+</sup>. Combining with S<sup>2-</sup> ions in the media, CdS semiconductors are therefore formed on the outer membrane of cells.</p></div>
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<h2>Light-driven nitrogen fixation in <em>E. coli</em> cells</h2>
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<p>When the system is exposed in light, electrons of  the CdS semiconductor conduct  transit, and CdS provide these  excited electrons to the electrons to Mo-Fe protein subunit of nitrogenase. Subsequently, the Mo-Fe protein utilizes the energy from these  electrons to reduce N<sub>2</sub>(dinitrogen) to NH<sub>3</sub>(ammonia). Finally, the semiconductor regains its lost electron from sacrificial electron donors.</p>
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<p>This design is of general applications as OmpA  protein is merely a surface display mechanism for&nbsp;<em>E. coli</em>, and PbrR can be  replaced with other proteins with different specificity. </p>
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<h2>Reaction device</h2>
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<p>We also designed a light-driven biohybrid reaction device to apply our system to practical use. After a few test, we proved our device to be quite practical. <a href="https://2018.igem.org/Team:Nanjing-China/Hardware">(see hardware for more details)</a> </p></div>
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
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            <p>Life Science Department<br />
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              #163 Xianlin Blvd, Qixia District<br />
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              Nanjing University<br />
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              Nanjing, Jiangsu Province<br />
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              P.R. of China<br />
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              Zip: 210046
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          <div class="f-word">
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            <p><strong><u>Email</u></strong></p>
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            <p>nanjing_china@163.com</p>
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Latest revision as of 03:02, 18 October 2018

Nanjing-China2018

Overview

Our design is composed of three parts: biosynthesis of CdS semiconductor, light-driven nitrogen fixation and a light-driven biohybrid reaction device. This system is the expansion of our previous project of hydrogen production (Nanjing-China 2016), and it proves that surface display mechanism is capable of being expanded to a general principle for light-driven biohybrid reactions.

Biosynthesis of CdS semiconductor on cell surface

To construct our light-driven system, we induce the in situ synthesis of CdS semiconductor on the E. coli cell surface. One key element of our system is fused protein OmpA-PbrR. OmpA (Outer membrane protein A) fixes the protein complex on outer cell membrane while PbrR (lead-specific binding protein) adsorbs Cd2+ in the environment and further forms CdS semiconductor on cell surface.

After Cd2+ ions are added into the culture, the ions specifically bind to PbrR protein contributing to the aggregation of Cd2+. Combining with S2- ions in the media, CdS semiconductors are therefore formed on the outer membrane of cells.

play
stop

Light-driven nitrogen fixation in E. coli cells

When the system is exposed in light, electrons of the CdS semiconductor conduct transit, and CdS provide these excited electrons to the electrons to Mo-Fe protein subunit of nitrogenase. Subsequently, the Mo-Fe protein utilizes the energy from these electrons to reduce N2(dinitrogen) to NH3(ammonia). Finally, the semiconductor regains its lost electron from sacrificial electron donors.

This design is of general applications as OmpA protein is merely a surface display mechanism for E. coli, and PbrR can be replaced with other proteins with different specificity.

play
stop

Reaction device

We also designed a light-driven biohybrid reaction device to apply our system to practical use. After a few test, we proved our device to be quite practical. (see hardware for more details)

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china