Difference between revisions of "Team:Nanjing-China/Safety"

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<title>Nanjing-China2018</title>
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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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</script>
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<div id="d">
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<div align="center">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<div id="HOME">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<div class="sub">
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<li>Including an "induced lethality" or "kill-switch" device</li>
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices"><font size="-1">Human_Practices</font></a></a></li></ul></div>
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</ul>
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<div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></ul></li></div>
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            <ul>
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    <li><a href="#over">Overview</a></li>
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            <li><a href="#R&P"><font size="-1">Risk& Precautions</font></a></li></ul>
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<div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></ul></li></div>
 
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<h3>Safe Lab Work</h3>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h3>Safe Shipment</h3>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
 
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<div id="for_judge" align="center"><div class="i"><ul><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges"><strong>For_judges</strong></a></ul></div></div>
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        <ul>
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  <li><a href="https://2018.igem.org/Team:Nanjing-China">N<font size="-1"><sub>2</sub></font> CHASER</a>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Team</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">PROJECT</a>
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    <ul>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate"><font size="-0.1">Demonstrate</font></a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Hardware">Hardware</a></li> 
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li>
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</ul>
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        </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a>
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        <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improve">Improve</a></li>
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</ul>
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            </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">MODELING</a></a>
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      </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">PRACTICES</a>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices"><font size="-1">Human_Practices</font></a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations"><font size="-0.1">Collaboration</font></a></li>
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</ul>
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    </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">NOTEBOOK</a></li>
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      </ul>
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  </div>
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  <div class="header"><img src="https://static.igem.org/mediawiki/2018/e/ec/T--Nanjing-China--title-Safty.png" width="100%" onload="MM_effectAppearFade(this, 1000, 0, 100, false);MM_effectBlind('HOME', 1000, '0%', '100%', true)"  >
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      <h2>Overview</h2>
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      <p> Biosafety is the precaution of large-scale loss of biological integrity in terms of ecology and human’s health.<sup>[1]</sup> These precaution mechanisms include conducting regular reviews of the biosafety in laboratory settings, following strict guidelines, employing an ongoing risk management assessment and so on. Downplaying or failing to fulfill such protocols can lead to augmented risk of exposure to biohazards or pathogens.</p>
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    </div>
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    <div class="word"  id="R&P">
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      <h2>Risk assessment& Precautions:</h2>
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      <p>Our project is to develop a sound whole-cell  photocatalytic nitrogen fixation system, using engineered <em>E.coli</em> BL21 or JM109.</p>
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        <p>We believe that risks are primarily embodied in three aspects and we have figured out appropriate solutions to all of them. Firstly,  some chemical and molecular biology reagents we use in experiments might be harmful and noxious. In order to reduce the adverse effect of these reagents on the environment and our health, we put the hazardous reagents in proper  storage, and carry out special procedures when dealing with them. For example, cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously  recycle the sewage containing Cd ion every time handling it. </p>
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        <p> Another safety concern comes from the inflammable and  explosive acetylene we use to test the activity of nitrogenase. In order to  avoid gas leakage, the gas cylinders which contains acetylene are operated correctly  and inspected regularly by special security staffs.</p>
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        <p>Thirdly, although both <em>E.coli</em> BL21 and JM109 are in Risk Group 1 and can cause no  disease to healthy adults, the genetically modified(GM) organisms can  potentially pose threats to the welfare of people and the environment if  released to real world. So we have very strict rules to prevent this from  happening. The waste produced in the lab are periodically collected, sterilized  and categorized by our team members and then recycled by professional chemical waste  recycling companies. These standard procedures guarantee that the GM organisms  would never escape from our labs.</p>
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      <p> We have inspectors to ensure our operation correctness and all team  members have received systematical biosafety training from relevant courses in advance. We all possess the skills in waste disposal, accident prevention,  emergency measures(such as  how to tackle fire, electric leakage and negligent wounds) and so on. During our experiments, we  stringently observed the biosafety guidelines issued both by our university and  WHO. </p>
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</div>
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<div class="word">
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    <p><em>Biosafety and the environment:An introduction to the Cartagena Protocol on Biosafety. GE.03-01836/E. United Nations Environment Programme. p. 8.</em></p>
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<div class="footer">
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
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            <p>Life Science Department<br />
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              #163 Xianlin Blvd, Qixia District<br />
 +
              Nanjing University<br />
 +
              Nanjing, Jiangsu Province<br />
 +
              P.R. of China<br />
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              Zip: 210046
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            </p></div>
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          <div class="f-word">
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            <p><strong><u>Email</u></strong></p>
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            <p>nanjing_china@163.com</p>
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            <p><strong><u>Social media</u></strong></p>
 +
            <p>Twitter button: <br/>
 +
            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
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Latest revision as of 03:38, 18 October 2018

Nanjing-China2018

Overview

Biosafety is the precaution of large-scale loss of biological integrity in terms of ecology and human’s health.[1] These precaution mechanisms include conducting regular reviews of the biosafety in laboratory settings, following strict guidelines, employing an ongoing risk management assessment and so on. Downplaying or failing to fulfill such protocols can lead to augmented risk of exposure to biohazards or pathogens.

Risk assessment& Precautions:

Our project is to develop a sound whole-cell photocatalytic nitrogen fixation system, using engineered E.coli BL21 or JM109.

We believe that risks are primarily embodied in three aspects and we have figured out appropriate solutions to all of them. Firstly, some chemical and molecular biology reagents we use in experiments might be harmful and noxious. In order to reduce the adverse effect of these reagents on the environment and our health, we put the hazardous reagents in proper storage, and carry out special procedures when dealing with them. For example, cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously recycle the sewage containing Cd ion every time handling it.

Another safety concern comes from the inflammable and explosive acetylene we use to test the activity of nitrogenase. In order to avoid gas leakage, the gas cylinders which contains acetylene are operated correctly and inspected regularly by special security staffs.

Thirdly, although both E.coli BL21 and JM109 are in Risk Group 1 and can cause no disease to healthy adults, the genetically modified(GM) organisms can potentially pose threats to the welfare of people and the environment if released to real world. So we have very strict rules to prevent this from happening. The waste produced in the lab are periodically collected, sterilized and categorized by our team members and then recycled by professional chemical waste recycling companies. These standard procedures guarantee that the GM organisms would never escape from our labs.

We have inspectors to ensure our operation correctness and all team members have received systematical biosafety training from relevant courses in advance. We all possess the skills in waste disposal, accident prevention, emergency measures(such as how to tackle fire, electric leakage and negligent wounds) and so on. During our experiments, we stringently observed the biosafety guidelines issued both by our university and WHO.

Biosafety and the environment:An introduction to the Cartagena Protocol on Biosafety. GE.03-01836/E. United Nations Environment Programme. p. 8.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china