Difference between revisions of "Team:UiOslo Norway"

 
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<h1> UiOslo Norway - Canditect </h1>
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<p>Welcome to our wiki, which is under development. Check back later!</p>
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<img src="https://static.igem.org/mediawiki/2018/7/7f/T--UiOslo_Norway--Canditect.png" class="front">
  
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<h3 style="text-align:center;">Fast detection of vulvovaginal <em>Candida albicans</em> </br>infections using CRISPR/dCas9</h3>
  
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<img class="front" src="https://static.igem.org/mediawiki/2018/3/33/T--UiOslo_Norway--Concept.png" alt="Concept graphic">
  
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<h3>Before you start</h3>
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<h3 style="text-align: center; margin-left: auto; margin-right: right;">UiOslo Norway</h3>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<img class="imgshadow teampic" src="https://static.igem.org/mediawiki/2018/b/ba/T--UiOslo_Norway--teamwin.jpg" alt="Team picture with award">
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<h3 style="text-align:center; text-decoration: italic;">Awarded Best Diagnostic Project</br>Nominated Best Presentation</br><i style="color: gold;"class="fas fa-medal"></i></h3>
 
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<p style="text-align:justify;"> During their lifetime 75 % of women will experience a <em>Candida albicans</em> (<em>C. albicans</em>) infection, one of the most common vulvovaginal yeast infections. Currently there are no fast methods to detect whether an infection is caused by <em>C. albicans</em> or not. In addition, women may purchase over-the-counter antimycotics without knowing the cause of their infection. This contributes to the rise of antimycotic resistance, making treatment of future infections more difficult.
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</p>
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<p style="text-align:justify;"><a href="https://2018.igem.org/Team:UiOslo_Norway/Attributions#inspiration">Based on previous projects</a>, UiOslo Norway aims to develop a fast detection kit for <em>C. albicans</em> infections, using CRISPR/dCas9. Upon a suspected infection, a vaginal sample will be treated with glucanase to selectively lyse yeast cells walls, exposing the fungal DNA. Afterwards, modified dCas9 enzymes fused with split β-lactamase are added. Using specifically designed guide RNAs, the dCas9 complexes bind adjacently on <em>C. albicans</em> specific DNA sequences. This activates the β-lactamase to cleave its substrate nitrocefin, producing a colored product indicating the presence of <em>C. albicans</em> DNA.
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<p style="text-align: center;">Contact information: uioslonorway (at) gmail (dot) com</p>
  
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<a href="https://www.uio.no/english/">
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  <img class="logo" src="https://static.igem.org/mediawiki/2018/3/32/T--UiOslo_Norway--UiO.png" target="_blank" alt="University of Oslo">
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</a>
  
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<a href="https://twistbioscience.com/">
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  <img class="logo" src="https://static.igem.org/mediawiki/2018/e/ea/T--UiOslo_Norway--Twist.png" target="_blank" alt="Twist Biosciences">
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<a href="https://www.uio.no/english/research/strategic-research-areas/life-science/">
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  <img class="logo" src="https://static.igem.org/mediawiki/2018/7/72/T--UiOslo_Norway--lifescience.png" target="_blank" alt="UiO: Life Sciences">
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  <img class="logo" src="https://static.igem.org/mediawiki/2018/a/ae/T--UiOslo_Norway--IDT.png" target="_blank" alt="Integrated DNA Technologies">
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<h3> Uploading pictures and files </h3>
 
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
 
  
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<a href="https://digitallifenorway.org/gb/">
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  <img class="logo" src="https://static.igem.org/mediawiki/2018/2/2a/T--UiOslo_Norway--CDL.png" target="_blank" alt="Centre for Digital Life Norway">
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<a href="https://www.biokjemisk.no/">
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  <img class="logo" src="https://static.igem.org/mediawiki/2018/e/e2/T--UiOslo_Norway--NBS.png" target="_blank" alt="Norwegian Biochemical Society">
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UPLOAD FILES
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<h3> Wiki template information </h3>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
  
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<h3>Tips</h3>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
 
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 09:26, 9 November 2018

Team members Collaboration
Description Design Results Demonstrate
Experiments Notebook Interlab
Parts Overview Basic Parts
Human Practices Integrated Human Practices Education & Engagement
Attributions Sponsors
Judging Form
Medal Criteria
Safety

Fast detection of vulvovaginal Candida albicans
infections using CRISPR/dCas9

Concept graphic

UiOslo Norway

Team picture with award

Awarded Best Diagnostic Project
Nominated Best Presentation

During their lifetime 75 % of women will experience a Candida albicans (C. albicans) infection, one of the most common vulvovaginal yeast infections. Currently there are no fast methods to detect whether an infection is caused by C. albicans or not. In addition, women may purchase over-the-counter antimycotics without knowing the cause of their infection. This contributes to the rise of antimycotic resistance, making treatment of future infections more difficult.

Based on previous projects, UiOslo Norway aims to develop a fast detection kit for C. albicans infections, using CRISPR/dCas9. Upon a suspected infection, a vaginal sample will be treated with glucanase to selectively lyse yeast cells walls, exposing the fungal DNA. Afterwards, modified dCas9 enzymes fused with split β-lactamase are added. Using specifically designed guide RNAs, the dCas9 complexes bind adjacently on C. albicans specific DNA sequences. This activates the β-lactamase to cleave its substrate nitrocefin, producing a colored product indicating the presence of C. albicans DNA.

Contact information: uioslonorway (at) gmail (dot) com