Difference between revisions of "Team:Evry Paris-Saclay/Parts"

 
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<div class="contentEvry">
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<h1>PARTS</h1>
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<h2>Titre de niveau 2</h2>
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<h3>Titre de niveau 3</h3>
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<h1 id="overview">Parts</h1>
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<p style="font-family: 'Cantarell', sans-serif; font-size: 20px;">Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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<body>
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ADD PARTS
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<div class="sidenav">
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</a>
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  <!--<a href="#overview">► Overview</a>-->
 +
  <a href="#basic_parts">► Basic Parts</a>
 +
  <a href="#composite_parts">► Composite Parts</a>
 +
  <a href="#part_collection">► Part Collection</a>
 
</div>
 
</div>
 +
<div class="contentEvry">
  
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</div>
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<h2 class="anchor" style="font-weight:800; text-align:center;" id='basic_parts'>BASIC PARTS</h2>
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</div>
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<p>During this project, our team designed the following basic parts:</p>
 +
<br>
 +
<h3 style="font-weight:800;">Coding sequences</h3>
  
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<div class="column third_size">
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<table>
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<div class="highlight decoration_A_full">
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  <tr>
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<h3>Inspiration</h3>
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      <th>Part Number</th>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
      <th>Part Name</th>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675000 " target=__blank">BBa_K2675000 </a></td>
 +
      <td> AimR of phage phi3T</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675001" target=__blank">BBa_K2675001 </a></td>
 +
      <td> AimP of phage phi3T</td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675002 " target=__blank">BBa_K2675002 </a></td>
 +
      <td> OmpA-SAIRGA </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675003 " target=__blank">BBa_K2675003 </a></td>
 +
      <td> PelB-SAIRGA </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675004 " target=__blank">BBa_K2675004 </a></td>
 +
      <td> TAT-SAIRGA </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675005 " target=__blank">BBa_K2675005 </a></td>
 +
      <td> sfGFP </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675006 " target=__blank">BBa_K2675006 </a></td>
 +
      <td> sfGFP-LVAtag </td>
 +
  </tr>
 +
</table>
  
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
−
</ul>
 
−
</div>
 
−
</div>
 
  
−
<div class="clear extra_space"></div>
+
<br></br>
 +
<h3 style="font-weight:800; ">Ribosomal binding sites (RBS)</h3>
  
−
<div class="column full_size">
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<table>
 +
  <tr>
 +
      <th>Part Number</th>
 +
      <th>Part Name</th>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675010 " target=__blank">BBa_K2675010 </a></td>
 +
      <td> Synthetic RBS designed for AimR of Phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675011 " target=__blank">BBa_K2675011 </a></td>
 +
      <td> Synthetic RBS designed for AimP of Phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675012 " target=__blank">BBa_K2675012 </a></td>
 +
      <td> Synthetic RBS designed for OmpA-SAIRGA </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675013 " target=__blank">BBa_K2675013 </a></td>
 +
      <td> Synthetic RBS designed for PelB-SAIRGA </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675014 " target=__blank">BBa_K2675014 </a></td>
 +
      <td> Synthetic RBS designed for TAT-SAIRGA </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675015 " target=__blank">BBa_K2675015 </a></td>
 +
      <td> Synthetic RBS designed for sfGFP </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675016 " target=__blank">BBa_K2675016 </a></td>
 +
      <td> Synthetic RBS designed for sfGFP </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675017 " target=__blank">BBa_K2675017 </a></td>
 +
      <td> Synthetic RBS designed for sfGFP </td>
 +
  </tr>
 +
</table>
  
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
<br></br>
−
<p>The information needed to initially create a part on the Registry is:</p>
+
<h3 style="font-weight:800; ">Promoters</h3>
−
<ul>
+
<table>
−
<li>Part Name</li>
+
  <tr>
−
<li>Part type</li>
+
      <th>Part Number</th>
−
<li>Creator</li>
+
      <th>Part Name</th>
−
<li>Sequence</li>
+
  </tr>
−
<li>Short Description (60 characters on what the DNA does)</li>
+
  <tr>
−
<li>Long Description (Longer description of what the DNA does)</li>
+
      <td><a href="http://parts.igem.org/Part:BBa_K2675020 " target=__blank">BBa_K2675020 </a></td>
−
<li>Design considerations</li>
+
      <td> pAimX(full) promoter of phage phi3T </td>  
−
</ul>
+
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675021 " target=__blank">BBa_K2675021 </a></td>
 +
      <td> pAimX(short) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675022 " target=__blank">BBa_K2675022 </a></td>
 +
      <td> pAimX(short)-J23110 hybrid promoter </td>  
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675023 " target=__blank">BBa_K2675023 </a></td>
 +
      <td> pAimX(full-1)-J23110 hybrid promoter </td>  
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675024 " target=__blank">BBa_K2675024 </a></td>
 +
      <td> pAimX(full-2)-J23110 hybrid promoter </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675025 " target=__blank">BBa_K2675025 </a></td>
 +
      <td> pAimX(full-3)-J23110 hybrid promoter </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675026 " target=__blank">BBa_K2675026 </a></td>
 +
      <td> J23110 truncated promoter (-10 only) + cloning Scar </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675090 " target=__blank">BBa_K2675090 </a></td>
 +
      <td> pAimX(full-noTerminator-v1) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675091 " target=__blank">BBa_K2675091 </a></td>
 +
      <td> pAimX(full-noTerminator-v2) promoter of phage phi3T </td>  
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675092 " target=__blank">BBa_K2675092 </a></td>
 +
      <td> pAimX(full-noTerminator-v3) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675093 " target=__blank">BBa_K2675093 </a></td>
 +
      <td> pAimX(full-noTerminator-v4) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675094 " target=__blank">BBa_K2675094 </a></td>
 +
      <td> pAimX(full-noTerminator-v5) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675095 " target=__blank">BBa_K2675095 </a></td>
 +
      <td> pAimX(full-noTerminator-v6) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675096 " target=__blank">BBa_K2675096 </a></td>
 +
      <td> pAimX(full-noTerminator-v7) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675097 " target=__blank">BBa_K2675097 </a></td>
 +
      <td> pAimX(full-noTerminator-v8) promoter of phage phi3T </td>
 +
  </tr>
 +
</table>
  
−
<p>
+
<br></br>
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
<h3 style="font-weight:800; ">Terminators</h3>
 +
<table>
 +
  <tr>
 +
      <th>Part Number</th>
 +
      <th>Part Name</th>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675030 " target=__blank">BBa_K2675030 </a></td>
 +
      <td> L3S2P56 Terminator </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675031 " target=__blank">BBa_K2675031 </a></td>
 +
      <td> L3S2P21 Terminator </td>
 +
  </tr>
 +
</table>
  
−
</div>
+
<br></br>
 +
<h3 style="font-weight:800;">And a Golden Gate adapter</h3>
 +
<table>
 +
  <tr>
 +
      <th>Part Number</th>
 +
      <th>Part Name</th>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675070 " target=__blank">BBa_K2675070
 +
      <td> Golden Gate adapter </td>
 +
  </tr>
 +
</table>
  
−
<div class="clear extra_space"></div>
+
<h2 class="anchor" style="font-weight:800; text-align:center;" id='composite_parts'>COMPOSITE PARTS</h2>
−
<div class="line_divider"></div>
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−
<div class="clear extra_space"></div>
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−
<div class="column full_size">
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<p>During this project, our team designed the following composite parts:</p>
−
<h3>Part Table </h3>
+
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
<table>
 +
  <tr>
 +
      <th>Part Number</th>
 +
      <th>Part Name</th>
 +
  </tr>
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675040 " target=__blank">BBa_K2675040 </a></td>
 +
      <td> AimR of phage phi3T expression cassette </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675041 " target=__blank">BBa_K2675041 </a></td>
 +
      <td> AimP of phage phi3T expression cassette </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675042 " target=__blank">BBa_K2675042 </a></td>
 +
      <td> OmpA-SAIRGA expression cassette </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675043 " target=__blank">BBa_K2675043 </a></td>
 +
      <td> PelB-SAIRGA expression cassette </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675044 " target=__blank">BBa_K2675044 </a></td>
 +
      <td> TAT-SAIRGA expression cassette </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675080 " target=__blank">BBa_K2675080 </a></td>
 +
      <td> Golden Gate adapter + sfGFP </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675081 " target=__blank">BBa_K2675081 </a></td>
 +
      <td> Golden Gate adapter + sfGFP-LVAtag </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675050 " target=__blank">BBa_K2675050 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675051 " target=__blank">BBa_K2675051 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(short) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675052 " target=__blank">BBa_K2675052 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(short)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675053 " target=__blank">BBa_K2675053 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675054 " target=__blank">BBa_K2675054 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675055 " target=__blank">BBa_K2675055 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675056 " target=__blank">BBa_K2675056 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675057 " target=__blank">BBa_K2675057 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675058 " target=__blank">BBa_K2675058 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(short) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675059 " target=__blank">BBa_K2675059 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 truncated promoter (-10 only) and pAimX(short) promoter of phage phi3T
 +
</td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675190 " target=__blank">BBa_K267190 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v1) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675191 " target=__blank">BBa_K2675191 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v2) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675192 " target=__blank">BBa_K2675192 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v3) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675193 " target=__blank">BBa_K2675193 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v4) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675194 " target=__blank">BBa_K2675194 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v5) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675195 " target=__blank">BBa_K2675195 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v6) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675196 " target=__blank">BBa_K2675196 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v7) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675197 " target=__blank">BBa_K2675197 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v8) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675060 " target=__blank">BBa_K2675060 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675061 " target=__blank">BBa_K2675061 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(short) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675062 " target=__blank">BBa_K2675062 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(short)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675063 " target=__blank">BBa_K2675063 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675064 " target=__blank">BBa_K2675064 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675065 " target=__blank">BBa_K2675065 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675066 " target=__blank">BBa_K2675066 </a></td>
 +
      <td> sfGFP expression cassette under the control of J23110 promoter </td>
 +
  </tr> 
 +
</table>
  
−
</html>
 
−
<groupparts>iGEM18 Evry_Paris-Saclay</groupparts>
 
−
<html>
 
−
</div>
 
  
−
<div class="column full_size">
+
<h2 class="anchor" style="font-weight:800; text-align:center;" id='part_collection'>PART COLLECTION</h2>
−
<h1 id="basic_parts">Basic Parts</h1>
+
−
<p>
+
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
+
−
</p>
+
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
+
<p style="font-size:15px;">In our project we tried to repurpose a peptide communication machinery from a phage of the Gram-positive <em>Bacillus subtilis</em> to the cells of Gram-negative <em>Escherichia coli</em>, and quickly discovered that crossing the inter-species barrier in bioengineering is a challenging task. This Part Collection consists of a series of promoter variants designed to debug the mechanism of transcriptional regulation in the pAimX promoter that is key to the signalling system. The Collection helped us successfully identify an internal terminator, investigate a potential anti-termination mechanism, and build a hybrid promoter that converts the native transcriptional activator (AimR) into a transcriptional repressor.</p>
−
</div>
+
<table>
 +
  <tr>
 +
      <th>Part Number</th>
 +
      <th>Part Name</th>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675020 " target=__blank">BBa_K2675020 </a></td>
 +
      <td> pAimX(full) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675021 " target=__blank">BBa_K2675021 </a></td>
 +
      <td> pAimX(short) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675022 " target=__blank">BBa_K2675022 </a></td>
 +
      <td> pAimX(short)-J23110 hybrid promoter </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675023 " target=__blank">BBa_K2675023 </a></td>
 +
      <td> pAimX(full-1)-J23110 hybrid promoter </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675024 " target=__blank">BBa_K2675024 </a></td>
 +
      <td> pAimX(full-2)-J23110 hybrid promoter </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675025 " target=__blank">BBa_K2675025 </a></td>
 +
      <td> pAimX(full-3)-J23110 hybrid promoter </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675090 " target=__blank">BBa_K2675090 </a></td>
 +
      <td> pAimX(full-noTerminator-v1) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675091 " target=__blank">BBa_K2675091 </a></td>
 +
      <td> pAimX(full-noTerminator-v2) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675092 " target=__blank">BBa_K2675092 </a></td>
 +
      <td> pAimX(full-noTerminator-v3) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675093 " target=__blank">BBa_K2675093 </a></td>
 +
      <td> pAimX(full-noTerminator-v4) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675094 " target=__blank">BBa_K2675094 </a></td>
 +
      <td> pAimX(full-noTerminator-v5) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675095 " target=__blank">BBa_K2675095 </a></td>
 +
      <td> pAimX(full-noTerminator-v6) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675096 " target=__blank">BBa_K2675096 </a></td>
 +
      <td> pAimX(full-noTerminator-v7) promoter of phage phi3T </td>
 +
  </tr>
 +
  <tr>
 +
      <td><a href="http://parts.igem.org/Part:BBa_K2675097 " target=__blank">BBa_K2675097 </a></td>
 +
      <td> pAimX(full-noTerminator-v8) promoter of phage phi3T </td>
 +
  </tr>
 +
</table>  
  
  
−
<div class="column full_size">
 
−
<div class="highlight decoration_background">
 
−
<h3>Note</h3>
 
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
 
−
</div>
 
−
</div>
 
  
−
<div class="column full_size">
+
<p style="font-size:15px;">When placed upstream of a reporter gene, promoters from this Part Collection showed variable transcription strengths and degrees of regulation that provided valuable insights for our project and facilitated the porting of the peptide communication machinery from <em>B. subtilis</em> to  <em>E. coli</em>.</p>
−
<h3>Best Basic Part Special Prize</h3>
+
<p style="font-size:15px;">To build this Part Collection the <a href="http://parts.igem.org/Part:BBa_K2675080" target=__blank">BBa_K2675080</a> and <a href="http://parts.igem.org/Part:BBa_K2675081" target=__blank">BBa_K2675081</a> were a key tool.</p>
  
−
<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
−
<br><br>
+
<table>
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
  <tr>
−
</div>
+
      <th>Part Number</th>
−
 
+
      <th>Part Name</th>
−
<div class="column full_size">
+
  </tr>
−
<h1 id='composite_parts'>Composite Parts</h1>
+
  <tr> 
−
 
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675050 " target=__blank">BBa_K2675050 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full) promoter of phage phi3T </td>  
−
<p>
+
  </tr>  
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
  <tr> 
−
</p>
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675051 " target=__blank">BBa_K2675051 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(short) promoter of phage phi3T </td>  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
+
  </tr> 
−
</div>
+
  <tr>  
−
 
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675052 " target=__blank">BBa_K2675052 </a></td>
−
<div class="column full_size">
+
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(short)-J23110 hybrid promoter </td>
−
<div class="highlight decoration_background">
+
  </tr> 
−
<h3>Note</h3>
+
  <tr> 
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675053 " target=__blank">BBa_K2675053 </a></td>
−
</div>
+
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter </td>
−
</div>
+
  </tr>  
−
 
+
  <tr> 
−
 
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675054 " target=__blank">BBa_K2675054 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter </td>  
−
<div class="column full_size">
+
  </tr>  
−
<h3>Best Composite Part Special Prize</h3>
+
  <tr> 
−
 
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675055 " target=__blank">BBa_K2675055 </a></td>
−
<p>To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
+
      <td> sfGFP-LVAtag expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter </td>
−
 
+
  </tr> 
−
<br><br>
+
  <tr> 
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675057 " target=__blank">BBa_K2675057 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full) promoter of phage phi3T </td>
−
</div>
+
  </tr> 
−
 
+
  <tr> 
−
<div class="column full_size">
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675058 " target=__blank">BBa_K2675058 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(short) promoter of phage phi3T </td>  
−
<h1 id='part_collection'> Part Collection </h1>
+
  </tr>  
−
<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
+
  <tr> 
−
 
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675059 " target=__blank">BBa_K2675059 </a></td>
−
<p>
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(short) promoter of phage phi3T </td>  
−
While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.  
+
  </tr> 
−
</p>
+
  <tr> 
−
</div>
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675190 " target=__blank">BBa_K2675190 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v1) promoter of phage phi3T </td>
−
 
+
  </tr> 
−
<div class="column full_size">
+
  <tr> 
−
<div class="highlight decoration_background">
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675191 " target=__blank">BBa_K2675191 </a></td>
−
<h3>Note</h3>
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v2) promoter of phage phi3T </td>
−
<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
+
  </tr> 
−
</div>
+
  <tr> 
−
</div>
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675192 " target=__blank">BBa_K2675192 </a></td>
−
 
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v3) promoter of phage phi3T </td>
−
<div class="column full_size">
+
  </tr>  
−
<h3>Best Part Collection Special Prize</h3>
+
  <tr>  
−
<p>To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
+
      <td><a href=" http://parts.igem.org/Part:BBa_K2675193 " target=__blank">BBa_K2675193 </a></td>
−
</div>
+
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v4) promoter of phage phi3T </td>  
 +
  </tr>  
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675194 " target=__blank">BBa_K2675194 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v5) promoter of phage phi3T </td>  
 +
  </tr>  
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675195 " target=__blank">BBa_K2675195 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v6) promoter of phage phi3T </td>  
 +
  </tr> 
 +
  <tr>  
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675196 " target=__blank">BBa_K2675196 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v7) promoter of phage phi3T </td>  
 +
  </tr>  
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675197 " target=__blank">BBa_K2675197 </a></td>
 +
      <td> sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v8) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675060 " target=__blank">BBa_K2675060 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full) promoter of phage phi3T </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675061 " target=__blank">BBa_K2675061 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(short) promoter of phage phi3T </td>  
 +
  </tr>  
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675062 " target=__blank">BBa_K2675062 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(short)-J23110 hybrid promoter </td>  
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675063 " target=__blank">BBa_K2675063 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675064 " target=__blank">BBa_K2675064 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter </td>
 +
  </tr> 
 +
  <tr> 
 +
      <td><a href=" http://parts.igem.org/Part:BBa_K2675065 " target=__blank">BBa_K2675065 </a></td>
 +
      <td> sfGFP expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter </td>
 +
  </tr>  
 +
</table>
  
 
</div>
 
</div>

Latest revision as of 20:06, 6 December 2018


TO CONTACT US
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+33 7 69 96 68 31
igemevry@gmail.com

© Copyright 2018
Design & Developpement by
IGEM EVRY GENOPOLE

► Basic Parts ► Composite Parts ► Part Collection

BASIC PARTS

During this project, our team designed the following basic parts:


Coding sequences

Part Number Part Name
BBa_K2675000 AimR of phage phi3T
BBa_K2675001 AimP of phage phi3T
BBa_K2675002 OmpA-SAIRGA
BBa_K2675003 PelB-SAIRGA
BBa_K2675004 TAT-SAIRGA
BBa_K2675005 sfGFP
BBa_K2675006 sfGFP-LVAtag


Ribosomal binding sites (RBS)

Part Number Part Name
BBa_K2675010 Synthetic RBS designed for AimR of Phi3T
BBa_K2675011 Synthetic RBS designed for AimP of Phi3T
BBa_K2675012 Synthetic RBS designed for OmpA-SAIRGA
BBa_K2675013 Synthetic RBS designed for PelB-SAIRGA
BBa_K2675014 Synthetic RBS designed for TAT-SAIRGA
BBa_K2675015 Synthetic RBS designed for sfGFP
BBa_K2675016 Synthetic RBS designed for sfGFP
BBa_K2675017 Synthetic RBS designed for sfGFP


Promoters

Part Number Part Name
BBa_K2675020 pAimX(full) promoter of phage phi3T
BBa_K2675021 pAimX(short) promoter of phage phi3T
BBa_K2675022 pAimX(short)-J23110 hybrid promoter
BBa_K2675023 pAimX(full-1)-J23110 hybrid promoter
BBa_K2675024 pAimX(full-2)-J23110 hybrid promoter
BBa_K2675025 pAimX(full-3)-J23110 hybrid promoter
BBa_K2675026 J23110 truncated promoter (-10 only) + cloning Scar
BBa_K2675090 pAimX(full-noTerminator-v1) promoter of phage phi3T
BBa_K2675091 pAimX(full-noTerminator-v2) promoter of phage phi3T
BBa_K2675092 pAimX(full-noTerminator-v3) promoter of phage phi3T
BBa_K2675093 pAimX(full-noTerminator-v4) promoter of phage phi3T
BBa_K2675094 pAimX(full-noTerminator-v5) promoter of phage phi3T
BBa_K2675095 pAimX(full-noTerminator-v6) promoter of phage phi3T
BBa_K2675096 pAimX(full-noTerminator-v7) promoter of phage phi3T
BBa_K2675097 pAimX(full-noTerminator-v8) promoter of phage phi3T


Terminators

Part Number Part Name
BBa_K2675030 L3S2P56 Terminator
BBa_K2675031 L3S2P21 Terminator


And a Golden Gate adapter

Part Number Part Name
BBa_K2675070 Golden Gate adapter

COMPOSITE PARTS

During this project, our team designed the following composite parts:

Part Number Part Name
BBa_K2675040 AimR of phage phi3T expression cassette
BBa_K2675041 AimP of phage phi3T expression cassette
BBa_K2675042 OmpA-SAIRGA expression cassette
BBa_K2675043 PelB-SAIRGA expression cassette
BBa_K2675044 TAT-SAIRGA expression cassette
BBa_K2675080 Golden Gate adapter + sfGFP
BBa_K2675081 Golden Gate adapter + sfGFP-LVAtag
BBa_K2675050 sfGFP-LVAtag expression cassette under the control of pAimX(full) promoter of phage phi3T
BBa_K2675051 sfGFP-LVAtag expression cassette under the control of pAimX(short) promoter of phage phi3T
BBa_K2675052 sfGFP-LVAtag expression cassette under the control of pAimX(short)-J23110 hybrid promoter
BBa_K2675053 sfGFP-LVAtag expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter
BBa_K2675054 sfGFP-LVAtag expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter
BBa_K2675055 sfGFP-LVAtag expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter
BBa_K2675056 sfGFP-LVAtag expression cassette under the control of J23110 promoter
BBa_K2675057 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full) promoter of phage phi3T
BBa_K2675058 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(short) promoter of phage phi3T
BBa_K2675059 sfGFP-LVAtag expression cassette under the control of J23110 truncated promoter (-10 only) and pAimX(short) promoter of phage phi3T
BBa_K267190 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v1) promoter of phage phi3T
BBa_K2675191 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v2) promoter of phage phi3T
BBa_K2675192 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v3) promoter of phage phi3T
BBa_K2675193 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v4) promoter of phage phi3T
BBa_K2675194 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v5) promoter of phage phi3T
BBa_K2675195 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v6) promoter of phage phi3T
BBa_K2675196 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v7) promoter of phage phi3T
BBa_K2675197 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v8) promoter of phage phi3T
BBa_K2675060 sfGFP expression cassette under the control of pAimX(full) promoter of phage phi3T
BBa_K2675061 sfGFP expression cassette under the control of pAimX(short) promoter of phage phi3T
BBa_K2675062 sfGFP expression cassette under the control of pAimX(short)-J23110 hybrid promoter
BBa_K2675063 sfGFP expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter
BBa_K2675064 sfGFP expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter
BBa_K2675065 sfGFP expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter
BBa_K2675066 sfGFP expression cassette under the control of J23110 promoter

PART COLLECTION

In our project we tried to repurpose a peptide communication machinery from a phage of the Gram-positive Bacillus subtilis to the cells of Gram-negative Escherichia coli, and quickly discovered that crossing the inter-species barrier in bioengineering is a challenging task. This Part Collection consists of a series of promoter variants designed to debug the mechanism of transcriptional regulation in the pAimX promoter that is key to the signalling system. The Collection helped us successfully identify an internal terminator, investigate a potential anti-termination mechanism, and build a hybrid promoter that converts the native transcriptional activator (AimR) into a transcriptional repressor.

Part Number Part Name
BBa_K2675020 pAimX(full) promoter of phage phi3T
BBa_K2675021 pAimX(short) promoter of phage phi3T
BBa_K2675022 pAimX(short)-J23110 hybrid promoter
BBa_K2675023 pAimX(full-1)-J23110 hybrid promoter
BBa_K2675024 pAimX(full-2)-J23110 hybrid promoter
BBa_K2675025 pAimX(full-3)-J23110 hybrid promoter
BBa_K2675090 pAimX(full-noTerminator-v1) promoter of phage phi3T
BBa_K2675091 pAimX(full-noTerminator-v2) promoter of phage phi3T
BBa_K2675092 pAimX(full-noTerminator-v3) promoter of phage phi3T
BBa_K2675093 pAimX(full-noTerminator-v4) promoter of phage phi3T
BBa_K2675094 pAimX(full-noTerminator-v5) promoter of phage phi3T
BBa_K2675095 pAimX(full-noTerminator-v6) promoter of phage phi3T
BBa_K2675096 pAimX(full-noTerminator-v7) promoter of phage phi3T
BBa_K2675097 pAimX(full-noTerminator-v8) promoter of phage phi3T

When placed upstream of a reporter gene, promoters from this Part Collection showed variable transcription strengths and degrees of regulation that provided valuable insights for our project and facilitated the porting of the peptide communication machinery from B. subtilis to E. coli.

To build this Part Collection the BBa_K2675080 and BBa_K2675081 were a key tool.

Part Number Part Name
BBa_K2675050 sfGFP-LVAtag expression cassette under the control of pAimX(full) promoter of phage phi3T
BBa_K2675051 sfGFP-LVAtag expression cassette under the control of pAimX(short) promoter of phage phi3T
BBa_K2675052 sfGFP-LVAtag expression cassette under the control of pAimX(short)-J23110 hybrid promoter
BBa_K2675053 sfGFP-LVAtag expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter
BBa_K2675054 sfGFP-LVAtag expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter
BBa_K2675055 sfGFP-LVAtag expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter
BBa_K2675057 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full) promoter of phage phi3T
BBa_K2675058 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(short) promoter of phage phi3T
BBa_K2675059 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(short) promoter of phage phi3T
BBa_K2675190 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v1) promoter of phage phi3T
BBa_K2675191 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v2) promoter of phage phi3T
BBa_K2675192 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v3) promoter of phage phi3T
BBa_K2675193 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v4) promoter of phage phi3T
BBa_K2675194 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v5) promoter of phage phi3T
BBa_K2675195 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v6) promoter of phage phi3T
BBa_K2675196 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v7) promoter of phage phi3T
BBa_K2675197 sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v8) promoter of phage phi3T
BBa_K2675060 sfGFP expression cassette under the control of pAimX(full) promoter of phage phi3T
BBa_K2675061 sfGFP expression cassette under the control of pAimX(short) promoter of phage phi3T
BBa_K2675062 sfGFP expression cassette under the control of pAimX(short)-J23110 hybrid promoter
BBa_K2675063 sfGFP expression cassette under the control of pAimX(full-1)-J23110 hybrid promoter
BBa_K2675064 sfGFP expression cassette under the control of pAimX(full-2)-J23110 hybrid promoter
BBa_K2675065 sfGFP expression cassette under the control of pAimX(full-3)-J23110 hybrid promoter