Difference between revisions of "Team:UCopenhagen"

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{{UCopenhagen}}
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{{UCopenhagen/Main}}
 
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         <h2>University of Copenhagen iGEM Team 2018 proudly presents</h2>
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         <h1>PharMARSy</h1>
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        <p>This year’s Copenhagen iGEM team is working on a protein production system for the colonization of Mars. Microgravity
 
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                on Mars induces muscle loss in Martians; to alleviate this, we will make pure human growth hormone using
 
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                our novel
 
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                <b>PharMARSy</b> system.
 
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                <b>PharMARSy</b> is a protein production system that uses the disease-causing bacterial injectisome (Type III
 
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                secretion system) as a molecular syringe to inject a specific protein, freshly made from a microbial factory,
 
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                through a biomimetic membrane.
 
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                <b>PharMARSy</b> allows for both production and purification of the protein - all in one.</p>
 
  
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        </br>
 
  
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        <p>Oh!
 
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                <b>PharMARSy</b> works on Earth too, and we can produce many other pure proteins with
 
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                <b>PharMARSy</b>! </p>
 
  
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         </br>
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<div class="pageContentWithBanner">
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    <div class="igem_2018_team_content">
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         <div class="igem_2018_team_column_wrapper">
  
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        <p> We will be adding more content to this Wiki over this summer. In the meantime, you may view our poster that we presented
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            <div class="column two_thirds_size">
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                at Nordic iGEM conference 2018 in Lund, where we won both the Judges prize and the iGEMers choice award.</p>
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        </br>
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        <img src="https://static.igem.org/mediawiki/2018/6/6e/T--UCopenhagen--PharMARSy-poster-NiC.jpg">
 
  
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                <h1 style="font-family: 'Aldrich', 'Exo 2', 'Arial Black', Gadget, sans-serif; font-size: 350%;">PharMARSy</h1>
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                <h4>A Simple Pharmaceutical Protein Production & Purification System on Mars</h4>
  
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</div>
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                <br>
  
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<!--
 
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                <div class="column full_size" >
 
  
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                <h3>Before you start</h3>
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                 <p>
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                 <p> Please read the following pages:</p>
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                    <em><b>"In order to truly explore space, astronauts will need the capability to produce needed
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                <ul>
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                            medications during their mission"</b> </em>- Virginia Wotring (Adjunct Associate Professor,
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                <li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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                    Center for Space Medicine and Department of Pharmacology and Chemical Biology, Baylor College of
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                <li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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                    Medicine).
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                <li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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                 </p>
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                 </ul>
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                </div>
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                <p>
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                    The race to the Red Planet has started and colonization could be in the near future! To make the
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                    colonization of Mars possible the first people will, of course, need to be able to survive and be
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                    self-sustaining in a place far from Earth. Astronauts will need access to medicine, but bringing it
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                    from the Earth is unsustainable. With our project, we aim to make a Portable Protein Production and
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                    Purification System that will solve the astronauts need for pharmaceutical protein by letting them
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                    produce their own medicine on site - no need to depend on the supply from Earth!
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                </p>
  
 
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                 <img src="https://static.igem.org/mediawiki/2018/3/3b/T--UCopenhagen--b1.png" alt="bubble1">
 
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                <h2>How does our system work? </h2>
  
  
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                <div class="column full_size" >
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                 <p>
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                <h3> Styling your wiki </h3>
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                    Our protein production system uses the bacterial injectisome (Type III Secretion System) as a
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                 <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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                    molecular syringe. Some pathogenic bacteria use the injectisome to secrete toxic proteins into
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                <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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                    mammalian cells, an example of such a bacteria is the Salmonella bacteria. When the bacteria want
 +
                    to secrete a protein through the injectisome, only specifically tagged proteins carrying an
 +
                    injectisome secretion signal will be able to be secreted. This means that only proteins tagged with
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                    that signal are able to get secreted through the injectisome. We want to take advantage of that
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                    feature by making the bacteria produce useful proteins for us and inject it out through a
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                    biomimetic membrane into an isolated <a href="https://2018.igem.org/Team:UCopenhagen/Model" target="_blank">collection
 +
                        chamber</a>. When the protein enters the isolated compartment it will theoretically be 100%
 +
                    pure and ready be collected and used for therapeutics. For a more detailed description of our
 +
                    protein production system, go <a href="https://2018.igem.org/Team:UCopenhagen/Description" target="_blank">here</a>
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                </p>
  
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                <h2>How does our system differ from conventional protein production?</h2>
  
  
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                <p>
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                    In conventional protein production the bacteria would produce the protein, but afterwards, we would
 +
                    need to go through a lot of steps in order to purify it. The process of purification takes time,
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                    lab equipment, chemicals, a lot of water and some of the protein goes to waste. However, the method
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                    allows for large-scale production.
 +
                </p>
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                <p>
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                    Due to the purification process, conventional protein production requires well-equipped labs and
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                    are therefore not portable.
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                </p>
  
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                <img src="https://static.igem.org/mediawiki/2018/c/c7/T--UCopenhagen--b3.png" alt="bubble3">
 
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                <h2>Why do we need a portable protein production system for long term space travel?</h2>
  
  
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                 <p>
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                    There are three very important problems with bringing medicine to space:
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                </p>
 +
                <ol>
  
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                <h3> Uploading pictures and files </h3>
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                    <li>Medicine expires after a while, so long-term space travel means that some of the medicine will
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                <p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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                        expire during the exploration.
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                    <li>The sheer amount of different medicine astronauts might need is vast which makes it difficult
 +
                        to predict what is needed to bring.
 +
                    <li>The cost to keep transporting medicine to sustain a colony would be unsustainable and very
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                        high. </li>
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                </ol>
  
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                <p>
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                    This all makes producing the medicine on-site the far the most attractive solution.
 +
                </p>
  
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                <p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
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                 <a href="https://2018.igem.org/Special:Upload">
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                 <img src="https://static.igem.org/mediawiki/2018/d/df/T--UCopenhagen--hp.png" alt="HP">
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                UPLOAD FILES
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                </a>
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                </div>
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            </div>
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            <div class="column third_size">
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                <div class="highlight decoration_background decoration_A_full">
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                    <h4>Achievements</h4>
 +
                    <br>
 +
                    <p>
 +
                        <i class="fas fa-medal fa-lg" style="color: gold"></i> Gold Medal from iGEM Competition 2018</p>
 +
                    <br>
 +
                    <p>
 +
                        <i class="fas fa-award fa-lg"></i> Nomination for Best Poster from iGEM Competition 2018</p>
 +
                    <br>
 +
                    <p>
 +
                        <i class="fas fa-trophy-alt fa-lg" style="color: gold"></i> Judges Award from Nordic iGEM
 +
                        Conference 2018</p>
 +
                    <br>
 +
                    <p>
 +
                        <i class="fas fa-trophy-alt fa-lg" style="color: gold"></i> iGEMers Choice Award from Nordic
 +
                        iGEM Conference 2018</p>
 +
                    <br>
 
                 </div>
 
                 </div>
 +
            </div>
  
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        </div>
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                <h3> Wiki template information </h3>
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    </div>
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                <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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    <script>
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        particlesJS("particles-js", {
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                 <h3> Editing your wiki </h3>
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            particles: {
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                 <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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                 number: {
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                 <p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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                <a href="https://2018.igem.org/wiki/index.php?title=Team:UCopenhagen&action=edit">
 
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                EDIT PAGE
 
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</html>
  
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{{UCopenhagen/Footer}}
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                <h3>Tips</h3>
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                <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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                <ul>
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                <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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                <li>Be clear about what you are doing and how you plan to do this.</li>
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                <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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                <li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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                <li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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                <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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                <li>Have lots of fun! </li>
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                <h3>Inspiration</h3>
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                <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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                <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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                <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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                <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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                <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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                <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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                <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 03:46, 7 December 2018

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PharMARSy

A Simple Pharmaceutical Protein Production & Purification System on Mars


"In order to truly explore space, astronauts will need the capability to produce needed medications during their mission" - Virginia Wotring (Adjunct Associate Professor, Center for Space Medicine and Department of Pharmacology and Chemical Biology, Baylor College of Medicine).

The race to the Red Planet has started and colonization could be in the near future! To make the colonization of Mars possible the first people will, of course, need to be able to survive and be self-sustaining in a place far from Earth. Astronauts will need access to medicine, but bringing it from the Earth is unsustainable. With our project, we aim to make a Portable Protein Production and Purification System that will solve the astronauts need for pharmaceutical protein by letting them produce their own medicine on site - no need to depend on the supply from Earth!

bubble1

How does our system work?

Our protein production system uses the bacterial injectisome (Type III Secretion System) as a molecular syringe. Some pathogenic bacteria use the injectisome to secrete toxic proteins into mammalian cells, an example of such a bacteria is the Salmonella bacteria. When the bacteria want to secrete a protein through the injectisome, only specifically tagged proteins carrying an injectisome secretion signal will be able to be secreted. This means that only proteins tagged with that signal are able to get secreted through the injectisome. We want to take advantage of that feature by making the bacteria produce useful proteins for us and inject it out through a biomimetic membrane into an isolated collection chamber. When the protein enters the isolated compartment it will theoretically be 100% pure and ready be collected and used for therapeutics. For a more detailed description of our protein production system, go here

bubble2

How does our system differ from conventional protein production?

In conventional protein production the bacteria would produce the protein, but afterwards, we would need to go through a lot of steps in order to purify it. The process of purification takes time, lab equipment, chemicals, a lot of water and some of the protein goes to waste. However, the method allows for large-scale production.

Due to the purification process, conventional protein production requires well-equipped labs and are therefore not portable.

bubble3

Why do we need a portable protein production system for long term space travel?

There are three very important problems with bringing medicine to space:

  1. Medicine expires after a while, so long-term space travel means that some of the medicine will expire during the exploration.
  2. The sheer amount of different medicine astronauts might need is vast which makes it difficult to predict what is needed to bring.
  3. The cost to keep transporting medicine to sustain a colony would be unsustainable and very high.

This all makes producing the medicine on-site the far the most attractive solution.

HP

Achievements


Gold Medal from iGEM Competition 2018


Nomination for Best Poster from iGEM Competition 2018


Judges Award from Nordic iGEM Conference 2018


iGEMers Choice Award from Nordic iGEM Conference 2018