Difference between revisions of "Team:Evry Paris-Saclay/Achievement"

 
(22 intermediate revisions by 3 users not shown)
Line 7: Line 7:
 
   width:100%;
 
   width:100%;
 
   text-align:center;
 
   text-align:center;
 +
  -moz-border-radius:15px;
 +
  -webkit-border-radius:15px;
 +
  border-radius:15px;
 
}
 
}
 +
 +
table > tr:first-child > td:first-child {
 +
border-top-left-radius: 15px;
 +
}
 +
 +
table > tr:first-child > td:last-child {
 +
border-top-right-radius: 15px;
 +
}
 +
 +
table > tr:last-child > td:first-child {
 +
border-bottom-left-radius: 15px;
 +
}
 +
 +
table > tr:last-child > td:last-child {
 +
border-bottom-right-radius: 15px;
 +
}
 +
 
table tr th{
 
table tr th{
 
   font-size: 16px;
 
   font-size: 16px;
Line 13: Line 33:
 
}
 
}
 
table tr td{
 
table tr td{
−
   font-size: 12px;
+
   font-size: 16px;
 
   width:600px;
 
   width:600px;
 
}
 
}
Line 20: Line 40:
 
<body>
 
<body>
 
<div class="contentEvry" style="text-align:center; padding-left:auto; padding-right:auto;">
 
<div class="contentEvry" style="text-align:center; padding-left:auto; padding-right:auto;">
 +
<p style="font-size: 20px; color: black; text-align:center;">This year, iGEM Evry Paris-Saclay won a <b>gold medal</b> and was nominated for <b>Best New Composite Part</b> Award (overgrad)</p>
 +
<img src="https://static.igem.org/mediawiki/2018/0/0b/T--Evry_Paris-Saclay--igem_logo_medaille_or.png" width="40%">
 +
<br/>
 +
<br/>
 
<h1  style="font-weight:800; text-align:center;">BRONZE MEDAL 🥉</h1>
 
<h1  style="font-weight:800; text-align:center;">BRONZE MEDAL 🥉</h1>
−
<table style="background-color:rgba(202,80,19,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Registration and Jamboree Attendance:</th>
 
       <th>Registration and Jamboree Attendance:</th>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td>We are ready to attend to the Giant Jamboree!</td>
+
       <td>We attended to the Giant Jamboree.</td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(202,80,19,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Competition Deliverables:</th>
 
       <th>Competition Deliverables:</th>
Line 36: Line 60:
 
       <td>All deliverables were met.</td>
 
       <td>All deliverables were met.</td>
 
   </tr>
 
   </tr>
−
</table style="background-color:rgba(202,80,19,0.5);">
+
</table>
 
<table>
 
<table>
 
   <tr>
 
   <tr>
Line 45: Line 69:
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(202,80,19,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Characterization / Contribution:</th>
 
       <th>Characterization / Contribution:</th>
Line 55: Line 79:
  
 
<h1  style="font-weight:800; text-align:center;">SILVER MEDAL 🥈</h1>
 
<h1  style="font-weight:800; text-align:center;">SILVER MEDAL 🥈</h1>
−
<table style="background-color:rgba(117,117,117,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Validated Part:</th>
 
       <th>Validated Part:</th>
Line 63: Line 87:
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(117,117,117,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Collaboration:</th>
 
       <th>Collaboration:</th>
Line 71: Line 95:
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(117,117,117,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Human Practices:</th>
 
       <th>Human Practices:</th>
Line 80: Line 104:
 
</table>
 
</table>
 
<h1  style="font-weight:800; text-align:center;">GOLD MEDAL 🥇</h1>
 
<h1  style="font-weight:800; text-align:center;">GOLD MEDAL 🥇</h1>
−
<table style="background-color:rgba(212,152,0,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Integrated Human Practices:</th>
 
       <th>Integrated Human Practices:</th>
Line 88: Line 112:
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(212,152,0,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Improve a Previous Part:</th>
 
       <th>Improve a Previous Part:</th>
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
−
       <td>By adding the OmpA secretion signal to the hexapeptide SAIRGA, we managed to obtain the secretion of the peptide by <I>E.coli</I>, which didn't occur with the original part (<a href=" http://parts.igem.org/Part:BBa_K2279001 " target=__blank">BBa_K2279001</a>), created to be used in <I>B.subtilis</I>. The secretion is a <I>sine qua non</I> condition for a functioning arbitrium system and, therefore, was needed to transfer this system in <I>E.coli</I>: <a href="https://2018.igem.org/Team:Evry_Paris-Saclay/Improve">https://2018.igem.org/Team:Evry_Paris-Saclay/Improve</a></td>
+
       <td>We created the <a href="http://parts.igem.org/Part:BBa_K2675042">BBa_K2675042</a> by adding the OmpA secretion signal to the hexapeptide SAIRGA and we managed to obtain the secretion of the peptide by <I>E.coli</I>, which didn't occur with the original part (<a href=" http://parts.igem.org/Part:BBa_K2279001 " target=__blank">BBa_K2279001</a>), created to be used in <I>B.subtilis</I>. The secretion is a <I>sine qua non</I> condition for a functioning arbitrium system and, therefore, was needed to transfer this system in <I>E.coli</I>: <a href="https://2018.igem.org/Team:Evry_Paris-Saclay/Improve">https://2018.igem.org/Team:Evry_Paris-Saclay/Improve</a>. This part (<a href="http://parts.igem.org/Part:BBa_K2675042">BBa_K2675042</a>) was nominated for the <b>Best New Composite Part</b> special prize.</td>
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(212,152,0,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Model Your Project:</th>
 
       <th>Model Your Project:</th>
Line 104: Line 128:
 
   </tr>
 
   </tr>
 
</table>
 
</table>
−
<table style="background-color:rgba(212,152,0,0.5);">
+
<table>
 
   <tr>
 
   <tr>
 
       <th>Demonstration of Your Work:</th>
 
       <th>Demonstration of Your Work:</th>
Line 114: Line 138:
  
 
<br/>
 
<br/>
−
<p style="font-size:15px;">Link to <a href=https://igem.org/2018_Judging_Form?team=Evry_Paris-Saclay>Judging form</a></p>
+
 
 
</div>
 
</div>
 
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
 
<br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br /><br />
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 00:44, 8 December 2018


TO CONTACT US
Genopole Campus 1, Batiment 6, 91030 Evry Cedex, France
+33 7 69 96 68 31
igemevry@gmail.com

© Copyright 2018
Design & Developpement by
IGEM EVRY GENOPOLE

This year, iGEM Evry Paris-Saclay won a gold medal and was nominated for Best New Composite Part Award (overgrad)



BRONZE MEDAL 🥉

Registration and Jamboree Attendance:
We attended to the Giant Jamboree.
Competition Deliverables:
All deliverables were met.
Attributions:
All the attributions and acknowledgements are available here: https://2018.igem.org/Team:Evry_Paris-Saclay/Attributions
Characterization / Contribution:
We successfully completed the InterLab Measurement Study: https://2018.igem.org/Team:Evry_Paris-Saclay/InterLab

SILVER MEDAL 🥈

Validated Part:
We have created a part that allows a quick engineering of a reporter gene thanks to a Golden Gate site (BBa_K2675081), another part that allows the production of a transcription factor (BBa_K2675040) and a third one that permit modulable expression of a tagged sfGFP thanks to the TF of the second part (BBa_K2675058).
Collaboration:
We have been involved in collaborations with 6 other iGEM teams in various fields such as Science, Communication and Education: https://2018.igem.org/Team:Evry_Paris-Saclay/Collaborations
Human Practices:
As our team is leading a foundational advance project, having a discussion with public representatives appeared to be complex. To address this problem, we decided to focus on meeting with professionals to find applications to our project, and on educating the general public about synthetic biology: https://2018.igem.org/Team:Evry_Paris-Saclay/Human_Practices

GOLD MEDAL 🥇

Integrated Human Practices:
After performing researches on our own and meeting various professionals, we managed to highlight important points of our project that needed a particular attention or modifications. It helped us in the determination of the exact type of communication system we would work on and the choice of our chassis and comforted us in our engagement in the education of the public: https://2018.igem.org/Team:Evry_Paris-Saclay/Human_Practices#integrated_human_practices
Improve a Previous Part:
We created the BBa_K2675042 by adding the OmpA secretion signal to the hexapeptide SAIRGA and we managed to obtain the secretion of the peptide by E.coli, which didn't occur with the original part (BBa_K2279001), created to be used in B.subtilis. The secretion is a sine qua non condition for a functioning arbitrium system and, therefore, was needed to transfer this system in E.coli: https://2018.igem.org/Team:Evry_Paris-Saclay/Improve. This part (BBa_K2675042) was nominated for the Best New Composite Part special prize.
Model Your Project:
We have used structural modelling to analyse in great detail the molecular interactions in the phage arbitrium system and to estimate whether the system would have been portable to E. coli. We have used the knowledge gained to guide the successful re-engineering of the aimR-aimP system into a flexible communication system for E. coli: https://2018.igem.org/Team:Evry_Paris-Saclay/Model
Demonstration of Your Work:
We have successfully transferred the ‘Arbitrium’ communication system from a Bacillus phage to E. coli. While doing so, we have engineered new “composite” promoters that convert the transcriptional activator AimR from Bacillus into a repressor in E. coli. We have also identified a putative intrinsic terminator in the pAimX(full) promoter sequence, and verified its terminator activity in E. coli. Further, we have ruled out a transcription antitermination mechanism at this terminator site. We also have promising data that indicates conditional regulation of AimR repressor activity by the small signalling peptide SAIRGA: https://2018.igem.org/Team:Evry_Paris-Saclay/Demonstrate