Difference between revisions of "Team:Nanjing-China/Team"

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         <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
 
         <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
 
             <li><a href="https://2018.igem.org/Team:Nanjing-China/Improved_Parts">Improved</a></li>
 
             <li><a href="https://2018.igem.org/Team:Nanjing-China/Improved_Parts">Improved</a></li>
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  </ul>
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</ul>
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        </li>
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            </li>
 
     <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
 
     <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
 
     <ul>
 
     <ul>
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   </div>
 
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    <div id="difinition">
 
     <div class="word">
 
     <div class="word">
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    <img src="https://static.igem.org/mediawiki/2017/9/9a/T-Nanjing-China-t-introduction.png" width="40%" />
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    <h2>The definition of nitrogen fixation</h2>
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    <h2>Abstract</h2>
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    <div class="word-2">
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    <p>Our team Nanjing-China 2018 intends to develop a whole-cell catalysis system targeted at developing industrial
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    <h3>•What is nitrogen fixation?</h3>
−
production to a more efficient level especially those dependent on metal catalyst. Compared with the traditional ones, biocatalyst can carry out reactions with low pollution, low energy consumption and high specificity. We manage to make our model organisms play the role of assembling metal ions and attach to the nucleation of them which thus form biohybrid whole-cell catalysis systems. As long as a collector is designed, we will make a portable whole-cell reacting system possible, which reactions with high efficiency.</p></div>
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<blockquote>
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<div class="word" align="center">
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  <p align="left"><font color="#FF9900"> Nitrogen → ammonia&nbsp;(NH<sub>3</sub>)</font>  or other molecules available to living organisms. </p>
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    <a href="https://2018.igem.org/Team:Nanjing-China/Members"><img src="https://static.igem.org/mediawiki/2018/5/57/T--Nanjing-China--all1.jpg" name="all1" width="80%" id="all1" onmouseover="MM_effectShake('click')" /></a>
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  </blockquote>
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    <div id="click"><font color="#CC3333"><p><h4><font size="120%">☝</font>Click the photo to see more details about our team</h4></p></font></div>
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        <div><img src="https://static.igem.org/mediawiki/2018/1/17/T--Nanjing-China--i-background-1.png" width="80%" /></div>
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</div>
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<div class="word-2">
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    <div><img src="https://static.igem.org/mediawiki/2018/2/2a/T--Nanjing-China--i-background-2.png" width="50%" /></div>
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    <h2>What is nitrogen fixation for?</h2>
 +
        <p align="left"> <font color="#339900">-global food supply <br/>   
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-reduce the use of chemical nitrogen fertilizers </font> </p>  
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</div>
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    <div class="word"><div class="word-background-block" style="width:100%;">   </div>
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    <h4>Nitrogen fixation is essential for life. </h4>
 
     </div>
 
     </div>
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    </div>
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    </div>
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    <div id="method">
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        <div class="word">
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  <h2>The methods of nitrogem fixation</h2>
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    <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-3.gif" width="80%" /></div>
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    <div class="word-2">
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    <h3>Nitrogen cycle</h3>
 +
    <p align="left"><font color="#FF6600">•Nitrogen fixation:</font><br/>
 +
          —N<sub>2 </sub>→ plants by bacteria<br/>
 +
    <font color="#FF6600">•Nitrification:</font><br/>
 +
      —ammonium → nitrite  → nitrate<br/>
 +
      —Absorbed by plants<br/>
 +
    <font color="#FF6600">•Denitrification:</font><br/>
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      —Release N to atmosphere</p>
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<div class="word-background-block" style="height:180px"></div>
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  </div>
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  </div>
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  <div class="word">
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      <div class="word-2">
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    <div class="word-background-block" style="height:80px; width:100%;"></div>
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    <h3>Haber-Bosch process </h3>
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    <p align="left"><font color="#FF6600">N2 + 3H<sub>2</sub> → 2NH<sub>3</sub></font>(ΔH° = −91.8 kJ)<br/>
 +
    <font color="#FF6600">High temperature<br/>
 +
High pressure<br />
 +
Need too much energy<br /></font><br/>
 +
                        </p>
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  </div>
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    <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-4.gif" width="80%" /></div>
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  </div>
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  <div class="word">
 +
  <div class="word-background-block"></div>
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  <h3>Biological nitrogen fixation</h3>
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    <div class="word-2" style="width:60%"><img src="https://static.igem.org/mediawiki/2018/2/20/T--Nanjing-China--i-background-5.png" width="90%" /></div>
 +
        <div class="word-2" style="width:40%" align="left">
 +
        <p><font color="#CC66FF">Mild reaction conditions</font></p>
 +
        <p><font color="#0033CC">Relatively inexpensive</font></p>
 +
        <p><font color="#996600">High efficiency </font></p>
 +
        <p><font color="#0099FF">Environmentally friendly</font></p>
 +
        <div class="word-background-block"></div>
 +
        </div>
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  </div>
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  </div>
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  <div id="choose">
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  <div class="word-background-block"></div>
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  <h2>The choose of nitrogenase</h2>
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  <div class="word">
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  <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/f/f5/T--Nanjing-China--i-background-6.png" width="90%" />
 +
  <p>Molybdenum <br />(MoFe)-dependent</p></div>
 +
  <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/a/a9/T--Nanjing-China--i-background-7.png" width="90%" />
 +
  <p>Vanadium <br />(VFe)-dependent</p></div>
 +
  <div class="word-3" ><img src="https://static.igem.org/mediawiki/2018/f/f0/T--Nanjing-China--i-background-8.png" width="85%" />
 +
  <p>Iron-only  <br />(FeFe)-dependent</p></div>
 +
  </div>
 +
  <div class="word">
 +
  <div class="word-background-block"></div><h4>MoFe has been studied extensively</h4>
 +
  </div>
 +
    <div class="word">
 +
  <div class="word-background-block"></div><h3>Nitrogen Fixation Gene Cluster </h3>
 +
  <img src="https://static.igem.org/mediawiki/2018/d/d5/T--Nanjing-China--i-background-9.png" width="85%" />
 +
  <h4>The minimal nif genes required for nitrogen fixation.</h4>
 +
  </div>
 +
  </div>
 +
  <div id="principle">
 +
  <div class="word">
 +
      <h2>General principle of biological nitrogen fixation </h2>
 +
    <table width="90%" border="1" cellspacing="1" cellpadding="1">
 +
      <tr>
 +
        <td><font color="#0033CC">01 Hydrolysis of ATP</font></td>
 +
        <td rowspan="2" width="65%"><div class="word-background-block" style="alignment-adjust:central"><img src="https://static.igem.org/mediawiki/2018/2/22/T--Nanjing-China--i-background-10.png" width="100%"/></div>
 +
          <p align="center">Chemical energy (ATP)→Solar energy</p></td>
 +
        <td><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
 +
      </tr>
 +
      <tr>
 +
        <td><font color="#CCCC00">02 Electron transfer </font></td>
 +
        <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
 +
      </tr>
 +
    </table>
 +
    </div>
 +
    <div class="word">
 +
    <div class="word-background-block"></div>
 +
      <table width="90%" border="1" cellspacing="1" cellpadding="1">
 +
      <tr>
 +
        <td><font color="#0033CC">01 Receive light signals</font></td>
 +
        <td rowspan="2" width="65%" style="alignment-adjust:central"><img src="https://static.igem.org/mediawiki/2018/e/e3/T--Nanjing-China--i-background-11.png" width="100%"/>
 +
        </td>
 +
        <td><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
 +
      </tr>
 +
      <tr>
 +
        <td><font color="#CCCC00">02 Electron transfer </font></td>
 +
        <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
 +
      </tr>
 +
    </table>
 +
    </div>
 +
    </div>
 +
  <div id="whole">
 +
  <div class="word">
 +
      <h2>The characteristics of whole-cell</h2>
 +
  <p><font size="110%" color="#CC6633">The property and advantage of whole-cells</font></p>
 +
  <p>•Fast  proliferation to enable large-scale application<br />
 +
  •Good  portability to permit on-site monitoring<br />
 +
  •Inexpensive  and easy to preserve<br />
 +
  •  Intracellular cascade reactions to amplify signals<br/>
 +
  •  Excellent specificity, sensitivity and stability</p>
 +
  </div>
 +
  </div>
 
   </div>
 
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Revision as of 06:57, 14 April 2018

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The definition of nitrogen fixation

•What is nitrogen fixation?

Nitrogen → ammonia (NH3) or other molecules available to living organisms.

What is nitrogen fixation for?

-global food supply
-reduce the use of chemical nitrogen fertilizers 

Nitrogen fixation is essential for life.

The methods of nitrogem fixation

Nitrogen cycle

•Nitrogen fixation:
     —N2 → plants by bacteria
•Nitrification:
     —ammonium → nitrite → nitrate
     —Absorbed by plants
•Denitrification:
     —Release N to atmosphere

Haber-Bosch process

N2 + 3H2 → 2NH3(ΔH° = −91.8 kJ)
High temperature
High pressure
Need too much energy

Biological nitrogen fixation

Mild reaction conditions

Relatively inexpensive

High efficiency

Environmentally friendly

The choose of nitrogenase

Molybdenum
(MoFe)-dependent

Vanadium
(VFe)-dependent

Iron-only
(FeFe)-dependent

MoFe has been studied extensively

Nitrogen Fixation Gene Cluster

The minimal nif genes required for nitrogen fixation.

General principle of biological nitrogen fixation

01 Hydrolysis of ATP

Chemical energy (ATP)→Solar energy

 03 MoFe protein forming complexes with low-potential donor
02 Electron transfer 04 Reduce N2 to NH3
01 Receive light signals 03 MoFe protein forming complexes with low-potential donor
02 Electron transfer 04 Reduce N2 to NH3

The characteristics of whole-cell

The property and advantage of whole-cells

•Fast proliferation to enable large-scale application
•Good portability to permit on-site monitoring
•Inexpensive and easy to preserve
• Intracellular cascade reactions to amplify signals
• Excellent specificity, sensitivity and stability

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china