Difference between revisions of "Team:Nanjing-China/Team"

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     <ul>  
 
     <ul>  
 
   <li><a href="https://2018.igem.org/Team:Nanjing-China">HOME</a>
 
   <li><a href="https://2018.igem.org/Team:Nanjing-China">HOME</a>
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     <div class="word">
 
     <div class="word">
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    <h2>The definition of nitrogen fixation</h2>
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    <img src="https://static.igem.org/mediawiki/2017/9/9a/T-Nanjing-China-t-introduction.png" width="40%" />
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    <div class="word-2">
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    <h2>Abstract</h2>
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    <h3>•What is nitrogen fixation?</h3>
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    <p>Our team Nanjing-China 2018 intends to develop a whole-cell catalysis system targeted at developing industrial
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<blockquote>
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production to a more efficient level especially those dependent on metal catalyst. Compared with the traditional ones, biocatalyst can carry out reactions with low pollution, low energy consumption and high specificity. We manage to make our model organisms play the role of assembling metal ions and attach to the nucleation of them which thus form biohybrid whole-cell catalysis systems. As long as a collector is designed, we will make a portable whole-cell reacting system possible, which reactions with high efficiency.</p></div>
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  <p align="left"><font color="#FF9900"> Nitrogen → ammonia&nbsp;(NH<sub>3</sub>)</font>  or other molecules available to living organisms. </p>
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<div class="word" align="center">
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  </blockquote>
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    <a href="https://2018.igem.org/Team:Nanjing-China/Members"><img src="https://static.igem.org/mediawiki/2018/5/57/T--Nanjing-China--all1.jpg" name="all1" width="80%" id="all1" onmouseover="MM_effectShake('click')" /></a>
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        <div><img src="https://static.igem.org/mediawiki/2018/1/17/T--Nanjing-China--i-background-1.png" width="80%" /></div>
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    <div id="click"><font color="#CC3333"><p><h4><font size="120%">☝</font>Click the photo to see more details about our team</h4></p></font></div>
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    <div><img src="https://static.igem.org/mediawiki/2018/2/2a/T--Nanjing-China--i-background-2.png" width="50%" /></div>
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    <h2>What is nitrogen fixation for?</h2>
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        <p align="left"> <font color="#339900">-global food supply <br/>   
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-reduce the use of chemical nitrogen fertilizers </font> </p>  
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    <div class="word"><div class="word-background-block" style="width:100%;">   </div>
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    <h4>Nitrogen fixation is essential for life. </h4>
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  <h2>The methods of nitrogem fixation</h2>
 
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    <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-3.gif" width="80%" /></div>
 
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    <div class="word-2">
 
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    <h3>Nitrogen cycle</h3>
 
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    <p align="left"><font color="#FF6600">•Nitrogen fixation:</font><br/>
 
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          —N<sub>2 </sub>→ plants by bacteria<br/>
 
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    <font color="#FF6600">•Nitrification:</font><br/>
 
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      —ammonium → nitrite  → nitrate<br/>
 
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      —Absorbed by plants<br/>
 
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    <font color="#FF6600">•Denitrification:</font><br/>
 
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      —Release N to atmosphere</p>
 
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    <h3>Haber-Bosch process </h3>
 
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    <p align="left"><font color="#FF6600">N2 + 3H<sub>2</sub> → 2NH<sub>3</sub></font>(ΔH° = −91.8 kJ)<br/>
 
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    <font color="#FF6600">High temperature<br/>
 
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High pressure<br />
 
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Need too much energy<br /></font><br/>
 
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                        </p>
 
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    <div class="word-2"><img src="https://static.igem.org/mediawiki/2018/9/9c/T--Nanjing-China--i-background-4.gif" width="80%" /></div>
 
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  <h3>Biological nitrogen fixation</h3>
 
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    <div class="word-2" style="width:60%"><img src="https://static.igem.org/mediawiki/2018/2/20/T--Nanjing-China--i-background-5.png" width="90%" /></div>
 
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        <div class="word-2" style="width:40%" align="left">
 
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        <p><font color="#CC66FF">Mild reaction conditions</font></p>
 
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        <p><font color="#0033CC">Relatively inexpensive</font></p>
 
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        <p><font color="#996600">High efficiency </font></p>
 
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        <p><font color="#0099FF">Environmentally friendly</font></p>
 
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        <div class="word-background-block"></div>
 
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        </div>
 
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  <div class="word-background-block"></div>
 
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  <h2>The choose of nitrogenase</h2>
 
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  <div class="word">
 
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  <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/f/f5/T--Nanjing-China--i-background-6.png" width="90%" />
 
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  <p>Molybdenum <br />(MoFe)-dependent</p></div>
 
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  <div class="word-3"><img src="https://static.igem.org/mediawiki/2018/a/a9/T--Nanjing-China--i-background-7.png" width="90%" />
 
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  <p>Vanadium <br />(VFe)-dependent</p></div>
 
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  <div class="word-3" ><img src="https://static.igem.org/mediawiki/2018/f/f0/T--Nanjing-China--i-background-8.png" width="85%" />
 
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  <p>Iron-only  <br />(FeFe)-dependent</p></div>
 
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  </div>
 
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  <div class="word">
 
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  <div class="word-background-block"></div><h4>MoFe has been studied extensively</h4>
 
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  </div>
 
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    <div class="word">
 
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  <div class="word-background-block"></div><h3>Nitrogen Fixation Gene Cluster </h3>
 
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  <img src="https://static.igem.org/mediawiki/2018/d/d5/T--Nanjing-China--i-background-9.png" width="85%" />
 
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  <h4>The minimal nif genes required for nitrogen fixation.</h4>
 
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  <div id="principle">
 
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  <div class="word">
 
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      <h2>General principle of biological nitrogen fixation </h2>
 
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    <table width="90%" border="1" cellspacing="1" cellpadding="1">
 
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      <tr>
 
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        <td><font color="#0033CC">01 Hydrolysis of ATP</font></td>
 
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        <td rowspan="2" width="65%"><div class="word-background-block" style="alignment-adjust:central"><img src="https://static.igem.org/mediawiki/2018/2/22/T--Nanjing-China--i-background-10.png" width="100%"/></div>
 
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          <p align="center">Chemical energy (ATP)→Solar energy</p></td>
 
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        <td><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
 
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      </tr>
 
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      <tr>
 
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        <td><font color="#CCCC00">02 Electron transfer </font></td>
 
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        <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
 
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        <td><font color="#0033CC">01 Receive light signals</font></td>
 
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        <td rowspan="2" width="65%" style="alignment-adjust:central"><img src="https://static.igem.org/mediawiki/2018/e/e3/T--Nanjing-China--i-background-11.png" width="100%"/>
 
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        <td><font color="#0099FF">03 MoFe protein forming complexes with low-potential donor</font></td>
 
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      <tr>
 
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        <td><font color="#CCCC00">02 Electron transfer </font></td>
 
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        <td><font color="#CC00FF">04 Reduce N2 to NH3 </font></td>
 
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      <h2>The characteristics of whole-cell</h2>
 
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  <p><font size="110%" color="#CC6633">The property and advantage of whole-cells</font></p>
 
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  <p>•Fast  proliferation to enable large-scale application<br />
 
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  •Good  portability to permit on-site monitoring<br />
 
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  •Inexpensive  and easy to preserve<br />
 
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  •  Intracellular cascade reactions to amplify signals<br/>
 
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  •  Excellent specificity, sensitivity and stability</p>
 
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Revision as of 09:42, 14 April 2018

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Abstract

Our team Nanjing-China 2018 intends to develop a whole-cell catalysis system targeted at developing industrial production to a more efficient level especially those dependent on metal catalyst. Compared with the traditional ones, biocatalyst can carry out reactions with low pollution, low energy consumption and high specificity. We manage to make our model organisms play the role of assembling metal ions and attach to the nucleation of them which thus form biohybrid whole-cell catalysis systems. As long as a collector is designed, we will make a portable whole-cell reacting system possible, which reactions with high efficiency.

☝Click the photo to see more details about our team

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china