Difference between revisions of "Team:Nanjing-China/Background"
| Line 221: | Line 221: | ||
<div id="menu"> | <div id="menu"> | ||
<ul> | <ul> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China" style="background-image:url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png)">HOME</a> | + | <li><a href="https://2018.igem.org/Team:Nanjing-China" style="background-image:"url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png")">HOME</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li> | ||
| Line 227: | Line 227: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2017.igem.org/Team:Nanjing-China/Parts" style="background-image:url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png)">PARTS</a> | + | <li><a href="https://2017.igem.org/Team:Nanjing-China/Parts" style="background-image:url("https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png")">PARTS</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li> | ||
| Line 233: | Line 233: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver" style="background-image:url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png)">JUDGE</a> | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver" style="background-image:url("https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png")">JUDGE</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li> | ||
| Line 240: | Line 240: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Design" style="background-image:url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png)">PROJECT</a> | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Design" style="background-image:url("https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png")">PROJECT</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li> | ||
| Line 249: | Line 249: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Team" style="background-image:url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png)">TEAM</a> | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Team" style="background-image:url("https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png")">TEAM</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Introduction</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Introduction</a></li> | ||
| Line 257: | Line 257: | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices" style="background-image:url(https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png)">OTHERS</a> | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices" style="background-image:url("https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png")">OTHERS</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li> | ||
Revision as of 06:53, 15 April 2018
The definition of nitrogen fixation
•What is nitrogen fixation?
Nitrogen → ammonia (NH3) or other molecules available to living organisms.
What is nitrogen fixation for?
-global food supply
-reduce the use of chemical nitrogen fertilizers
Nitrogen fixation is essential for life.
The methods of nitrogem fixation
Nitrogen cycle
•Nitrogen fixation:
—N2 → plants by bacteria
•Nitrification:
—ammonium → nitrite → nitrate
—Absorbed by plants
•Denitrification:
—Release N to atmosphere
Haber-Bosch process
N2 + 3H2 → 2NH3(ΔH° = −91.8 kJ)
High temperature
High pressure
Need too much energy
Biological nitrogen fixation
Mild reaction conditions
Relatively inexpensive
High efficiency
Environmentally friendly
The choose of nitrogenase
Molybdenum
(MoFe)-dependent
Vanadium
(VFe)-dependent
Iron-only
(FeFe)-dependent
MoFe has been studied extensively
Nitrogen Fixation Gene Cluster
The minimal nif genes required for nitrogen fixation.
General principle of biological nitrogen fixation
| 01 Hydrolysis of ATP | 03 MoFe protein forming complexes with low-potential donor | |
 Chemical energy (ATP)→Solar energy |
||
| 02 Electron transfer | 04 Reduce N2 to NH3 |
| 01 Receive light signals | 03 MoFe protein forming complexes with low-potential donor | |
 |
||
| 02 Electron transfer | 04 Reduce N2 to NH3 |
The characteristics of whole-cell
The property and advantage of whole-cells
•Fast proliferation to enable large-scale application
•Good portability to permit on-site monitoring
•Inexpensive and easy to preserve
• Intracellular cascade reactions to amplify signals
• Excellent specificity, sensitivity and stability
Address
Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046
nanjing_china@163.com
Social media
Twitter button:
iGEM Nanjing-China@nanjing_china
