Difference between revisions of "Team:Nanjing-China/Design"

(Prototype team page)
 
Line 1: Line 1:
−
{{Nanjing-China}}
+
<html xmlns="http://www.w3.org/1999/xhtml">
−
<html>
+
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<title>无标题文档</title>
 +
<style type="text/css">
 +
<!--
 +
#sideMenu,
 +
#top_title{
 +
    display: none;
 +
}
 +
#content{
 +
    width: 100%;
 +
    margin: 0px;
 +
    padding:0;
 +
background-color:block;
 +
}
 +
#HQ_page{ background-color:#FFF;
 +
background-image:url("https://static.igem.org/mediawiki/2018/6/6b/T--Nanjing-China--background-1.png");
 +
background-attachment:fixed;
 +
background-size:cover;
 +
background-repeat:no-repeat
 +
background-position:center;}
 +
#HQ_page p{ font-size:110%;}
 +
#HQ_page h3{ font-size:130%;}
 +
#HQ_page h2{ font-size:150%;}
 +
#HQ_page h1{ font-size:200%; font-family: "Comic Sans MS", cursive;}
 +
#HQ_page table{ border:none;
 +
background-color:rgba(0,51,204,0);}
 +
#HQ_page td{border:none;}
 +
body {
 +
font: 100%/1.4 Verdana, Arial, Helvetica, sans-serif;
 +
margin: 0;
 +
padding: 0;
 +
    background-color:#FFF;
 +
}
 +
a img {border: none;}
 +
table {
 +
    border-collapse: collapse;
 +
    border-spacing: 0;
 +
}
 +
.container {
 +
width: 100%;
 +
font-family: Verdana, Geneva, sans-serif;
 +
margin: 0 auto;
 +
alignment-adjust:central;
 +
}
 +
.header {
 +
position:relative;
 +
width:100%;
 +
font-family: Verdana, Geneva, sans-serif;
 +
alignment-adjust:central;
 +
z-index:2;
 +
}
 +
.contain {
 +
position: relative;
 +
top:-120px;
 +
width: 76%;
 +
max-width: 1100px;
 +
min-width: 700px;
 +
padding: 10px 0;
 +
alignment-adjust: central;
 +
background-color:rgba(0,51,204,0);
 +
}
 +
.contain ul, .contain ol {
 +
padding: 0 15px 15px 40px;
 +
}
 +
.footer {
 +
position:relative;
 +
clear: both;
 +
alignment-adjust:central;
 +
}
 +
#menu{
 +
position:fixed;
 +
left:50px;
 +
width: 770px;
 +
font-family: "Comic Sans MS", cursive;
 +
z-index:3;
 +
 +
}
 +
#menu ul{ margin: auto; padding:0; list-style:none; width:770px;}
 +
#menu ul li{ height:42px; line-height:42px; margin-left: 2px; margin-bottom: 2px; border-right:3px solid #666; border-bottom:2.8px solid #666; border-top:1.5px solid #666; border-left:1px solid #666; text-align:center; float:left; width:122px; color:#F90;}
 +
#menu ul li a{ color:#003; text-decoration:none; display:block;
 +
width:123px; height:42px;}
 +
#menu ul li a:hover{
 +
background-color: #999;
 +
color: #FFF;
 +
width: 124px;
 +
height: 46px;
 +
font-family:Verdana, Geneva, sans-serif;}
 +
#menu ul li ul{ display:none; width:124px; z-index:3;}
 +
#menu ul li.listshow ul{ display:block;}
 +
#menu ul li ul li{
 +
float: none;
 +
width: 122px;
 +
margin: 0;
 +
}
 +
#menu ul li ul li a{ background:none; width:124px;}
 +
#menu ul li ul li a:hover{ background:rgba(102,102,102,0.6); color:#FFFFFF; width:124px; height:45px; font-family:Verdana, Geneva, sans-serif;}
 +
#menu ul li a#on{ background:none;}
 +
.clear{clear:both;}
 +
#d{ left:85%; width:15%; position:fixed; z-index:10;}
 +
#HOME {
 +
position:relative;
 +
    top:auto;
 +
width: 115px;
 +
text-align:center;
 +
background:rgba(255,255,0,0.2);
 +
font-size:15px;
 +
alignment-adjust:central;
 +
font-family: "Palatino Linotype", "Book Antiqua", Palatino, serif;
 +
z-index:3;
 +
visibility: hidden;
 +
}
 +
#HOME .sub {
 +
background-color:rgba(102,102,204,0.5); height:50px; line-height:50px; border-bottom:2px solid #999; border-right:2.5px solid #666; text-align:center; float:left; width:115px;}
 +
#HOME .sub ul{ margin: auto; padding:0; list-style:none; width:115px;}
 +
#HOME .sub ul li{
 +
background-color:rgba(0,153,255,0.3); height:50px; line-height:50px; border-bottom:2px solid #999; border-right:2.5px solid #666; text-align:center; float:left; width:115px;}
 +
#HOME .sub ul li a:hover{background-color:#930; color:#CCC; height:50px; line-height:50px;}
 +
#HOME .sub ul li a{ text-decoration:none; color:#000;}
 +
#HOME ul{
 +
margin: auto; padding:0; list-style:0;}
 +
#HOME ul li{ background:rgba(102,102,153,0.3); height:37px; line-height:37px; border-bottom:2px solid #999; border-right:2.5px solid #666; text-align:center; float:left; width:115px; margin-right:1px; list-style:0;}
 +
#HOME ul li a{
 +
text-decoration: none;
 +
display:block;
 +
color:#003;
 +
width:115px;
 +
height:37px;
  
−
<div class="column full_size">
+
}
−
<h1>Design</h1>
+
#HOME ul li a:hover{  background:rgba(102,102,153,0.5); color:#FFFFFF; width:115px; height:40px;}
−
<p>
+
#HOME ul li a#on{ background:rgba(102,102,153,0.9);}
−
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
+
.f{ position:absolute; width:80%; left:10%; alignment-adjust:central; z-index:2;}
−
</p>
+
.f-word{ position:relative; width:30%; left:auto; height:230px; float:left;}
 +
.f-img{ position:relative; width:20%; height:120px; float:left;}
 +
#HQ_page .f p{ font-size:90%;}
 +
.container .footer div .f .f-word h3 {
 +
color: #000;
 +
font-family: Verdana, Arial, Helvetica, sans-serif;
 +
}
 +
.f-b{position:relative;}
 +
.word-background-block{ width:100%;
 +
float:left;}
 +
.word-1{ width:100%;
 +
float:left;}
 +
.word-2{ width:50%;
 +
float:left;}
 +
.word-3{ width:33%;
 +
float:left;}
 +
.word{
 +
position:relative;
 +
width:90%;
 +
float:left;
 +
background-color:rgba(0,51,204,0.4);
 +
padding:5%;}
 +
-->
 +
#Layer1 {
 +
position:absolute;
 +
left:0;
 +
top:0;
 +
z-index:1;
 +
width: 140px;
 +
visibility: hidden;
 +
height: 115px;
 +
}
 +
#Layer2 {
 +
position:absolute;
 +
left:0;
 +
top:0;
 +
width:150px;
 +
z-index:1;
 +
height: 115px;
 +
visibility: visible;
 +
}
 +
#Layer3 {
 +
position:absolute;
 +
left:54px;
 +
top:24px;
 +
width:40;
 +
z-index:2;
 +
visibility: hidden;
 +
}
 +
#Layer4 {
 +
position:absolute;
 +
left:279px;
 +
top:40px;
 +
width:160px;
 +
height:56px;
 +
z-index:3;
 +
visibility: visible;
 +
}
 +
#Layer5 {
 +
position:absolute;
 +
left:155px;
 +
top:167px;
 +
width:30;
 +
z-index:3;
 +
visibility: visible;
 +
}
 +
#Layer6 {
 +
position:absolute;
 +
left:352px;
 +
top:70px;
 +
width:50px;
 +
z-index:3;
 +
height: 50px;
 +
visibility: visible;
 +
}
 +
#Layer7 {
 +
position:absolute;
 +
left:367px;
 +
top:43px;
 +
width:70;
 +
z-index:3;
 +
height: 72px;
 +
visibility: visible;
 +
}
 +
#Layer8 {
 +
position:absolute;
 +
left:312px;
 +
top:24px;
 +
width:184px;
 +
z-index:2;
 +
height: 106px;
 +
visibility: visible;
 +
}
 +
#Layer-all1 {
 +
position:absolute;
 +
left:0;
 +
top:0;
 +
width:108px;
 +
height:64px;
 +
z-index:6;
 +
visibility: hidden;
 +
}
 +
#Layer11 {
 +
position:absolute;
 +
left:19px;
 +
top:74px;
 +
width:141px;
 +
height:86px;
 +
z-index:1;
 +
visibility: hidden;
 +
}
 +
#Layer12 {
 +
position:absolute;
 +
left:32px;
 +
top:68px;
 +
z-index:2;
 +
width: 60px;
 +
visibility: visible;
 +
}
 +
#Layer13 {
 +
position:absolute;
 +
left:82px;
 +
top:81px;
 +
z-index:2;
 +
width: 100px;
 +
height: 62px;
 +
visibility: visible;
 +
}
 +
#Layer14 {
 +
position:absolute;
 +
left:0px;
 +
top:50px;
 +
width:98px;
 +
height:76px;
 +
z-index:1;
 +
visibility: visible;
 +
}
 +
#Layer15 {
 +
position:absolute;
 +
left:148px;
 +
top:29px;
 +
width:130;
 +
z-index:2;
 +
height: 49px;
 +
visibility: visible;
 +
}
 +
#Layer16 {
 +
position:absolute;
 +
left:-5;
 +
top:55;
 +
width:171px;
 +
height:130px;
 +
z-index:2;
 +
visibility: visible;
 +
}
 +
#Layer17 {
 +
position:absolute;
 +
left:168px;
 +
top:158px;
 +
z-index:2;
 +
width: 130px;
 +
height: 67px;
 +
visibility: visible;
 +
}
 +
#Layer18 {
 +
position:absolute;
 +
left:0px;
 +
top:30px;
 +
z-index:2;
 +
width: 200px;
 +
height: 172px;
 +
visibility: visible;
 +
}
 +
#Layer-all2 {
 +
position:absolute;
 +
left:0;
 +
top:0;
 +
z-index:3;
 +
width: 500px;
 +
height: 308px;
 +
visibility: hidden;
 +
}
 +
#Layer21 {
 +
position:absolute;
 +
left:0px;
 +
top:120px;
 +
z-index:1;
 +
width: 200px;
 +
height: 125px;
 +
visibility: hidden;
 +
}
 +
#Layer22 {
 +
position:absolute;
 +
left:24px;
 +
top:101px;
 +
width:179px;
 +
height:74px;
 +
z-index:2;
 +
visibility: hidden;
 +
}
 +
#Layer23 {
 +
position:absolute;
 +
top:118px;
 +
width:200px;
 +
z-index:2;
 +
left: -5px;
 +
height: 169px;
 +
visibility: hidden;
 +
}
 +
#Layer24 {
 +
position:absolute;
 +
left:206px;
 +
top:87px;
 +
width:60px;
 +
z-index:2;
 +
height: 131px;
 +
visibility: visible;
 +
}
 +
#Layer25 {
 +
position:absolute;
 +
left:-9px;
 +
top:108px;
 +
width:206px;
 +
height:103px;
 +
z-index:2;
 +
visibility: visible;
 +
}
 +
#Layer26 {
 +
position:absolute;
 +
left:183px;
 +
top:10px;
 +
width:208px;
 +
height:166px;
 +
z-index:2;
 +
visibility: visible;
 +
}
 +
#Layer27 {
 +
position:absolute;
 +
left:192px;
 +
top:136px;
 +
width:114px;
 +
z-index:2;
 +
visibility: hidden;
 +
}
 +
#Layer28 {
 +
position:absolute;
 +
left:170px;
 +
top:138px;
 +
width:150px;
 +
z-index:3;
 +
height: 91px;
 +
visibility: visible;
 +
}
 +
.play{
 +
position:absolute;
 +
left:430px;
 +
top:44px;
 +
width:57px;
 +
height:28px;
 +
z-index:2;
 +
}
 +
.stop{
 +
position:absolute;
 +
left:438px;
 +
top:116px;
 +
width:44px;
 +
height:23px;
 +
z-index:2;
 +
}
 +
</style>
 +
<script type="text/javascript" src="https://2018.igem.org/Team:Nanjing-China/Javascript:1?action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript">
 +
function menuFix(){
 +
    var sfEles=document.getElementById("menu").getElementsByTagName("li");
 +
        for(var i=0;i<sfEles.length; i++){
 +
        sfEles[i].onmouseover=function(){
 +
            this.className +=(this.className.length>0?"":"")+"listshow";
 +
            }
 +
            sfEles[i].onmouseout=function(){
 +
            this.className =this.className.replace("listshow","");
 +
            }
 +
        }
 +
    }
 +
    window.onload= menuFix;
 +
function MM_changeProp(objId,x,theProp,theValue) { //v9.0
 +
  var obj = null; with (document){ if (getElementById)
 +
  obj = getElementById(objId); }
 +
  if (obj){
 +
    if (theValue == true || theValue == false)
 +
      eval("obj.style."+theProp+"="+theValue);
 +
    else eval("obj.style."+theProp+"='"+theValue+"'");
 +
  }
 +
}
 +
function MM_effectAppearFade(targetElement, duration, from, to, toggle)
 +
{
 +
Spry.Effect.DoFade(targetElement, {duration: duration, from: from, to: to, toggle: toggle});
 +
}
 +
function MM_effectBlind(targetElement, duration, from, to, toggle)
 +
{
 +
Spry.Effect.DoBlind(targetElement, {duration: duration, from: from, to: to, toggle: toggle});
 +
}
 +
-->design
 +
function MM_timelinePlay(tmLnName, myID) { //v1.2
 +
  //Copyright 1998, 1999, 2000, 2001, 2002, 2003, 2004 Macromedia, Inc. All rights reserved.
 +
  var i,j,tmLn,props,keyFrm,sprite,numKeyFr,firstKeyFr,propNum,theObj,firstTime=false;
 +
  if (document.MM_Time == null) MM_initTimelines(); //if *very* 1st time
 +
  tmLn = document.MM_Time[tmLnName];
 +
  if (myID == null) { myID = ++tmLn.ID; firstTime=true;}//if new call, incr ID
 +
  if (myID == tmLn.ID) { //if Im newest
 +
    setTimeout('MM_timelinePlay("'+tmLnName+'",'+myID+')',tmLn.delay);
 +
    fNew = ++tmLn.curFrame;
 +
    for (i=0; i<tmLn.length; i++) {
 +
      sprite = tmLn[i];
 +
      if (sprite.charAt(0) == 's') {
 +
        if (sprite.obj) {
 +
          numKeyFr = sprite.keyFrames.length; firstKeyFr = sprite.keyFrames[0];
 +
          if (fNew >= firstKeyFr && fNew <= sprite.keyFrames[numKeyFr-1]) {//in range
 +
            keyFrm=1;
 +
            for (j=0; j<sprite.values.length; j++) {
 +
              props = sprite.values[j];
 +
              if (numKeyFr != props.length) {
 +
                if (props.prop2 == null) sprite.obj[props.prop] = props[fNew-firstKeyFr];
 +
                else        sprite.obj[props.prop2][props.prop] = props[fNew-firstKeyFr];
 +
              } else {
 +
                while (keyFrm<numKeyFr && fNew>=sprite.keyFrames[keyFrm]) keyFrm++;
 +
                if (firstTime || fNew==sprite.keyFrames[keyFrm-1]) {
 +
                  if (props.prop2 == null) sprite.obj[props.prop] = props[keyFrm-1];
 +
                  else        sprite.obj[props.prop2][props.prop] = props[keyFrm-1];
 +
        } } } } }
 +
      } else if (sprite.charAt(0)=='b' && fNew == sprite.frame) eval(sprite.value);
 +
      if (fNew > tmLn.lastFrame) tmLn.ID = 0;
 +
  } }
 +
}
  
−
<p>
+
function MM_timelineStop(tmLnName) { //v1.2
−
This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:Nanjing-China/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
+
  //Copyright 1998, 1999, 2000, 2001, 2002, 2003, 2004 Macromedia, Inc. All rights reserved.
−
</p>
+
  if (document.MM_Time == null) MM_initTimelines(); //if *very* 1st time
 +
  if (tmLnName == null)  //stop all
 +
    for (var i=0; i<document.MM_Time.length; i++) document.MM_Time[i].ID = null;
 +
  else document.MM_Time[tmLnName].ID = null; //stop one
 +
}
  
−
</div>
+
function MM_timelineGoto(tmLnName, fNew, numGotos) { //v2.0
 +
  //Copyright 1998, 1999, 2000, 2001, 2002, 2003, 2004 Macromedia, Inc. All rights reserved.
 +
  var i,j,tmLn,props,keyFrm,sprite,numKeyFr,firstKeyFr,lastKeyFr,propNum,theObj;
 +
  if (document.MM_Time == null) MM_initTimelines(); //if *very* 1st time
 +
  tmLn = document.MM_Time[tmLnName];
 +
  if (numGotos != null)
 +
    if (tmLn.gotoCount == null) tmLn.gotoCount = 1;
 +
    else if (tmLn.gotoCount++ >= numGotos) {tmLn.gotoCount=0; return}
 +
  jmpFwd = (fNew > tmLn.curFrame);
 +
  for (i = 0; i < tmLn.length; i++) {
 +
    sprite = (jmpFwd)? tmLn[i] : tmLn[(tmLn.length-1)-i]; //count bkwds if jumping back
 +
    if (sprite.charAt(0) == "s") {
 +
      numKeyFr = sprite.keyFrames.length;
 +
      firstKeyFr = sprite.keyFrames[0];
 +
      lastKeyFr = sprite.keyFrames[numKeyFr - 1];
 +
      if ((jmpFwd && fNew<firstKeyFr) || (!jmpFwd && lastKeyFr<fNew)) continue; //skip if untouchd
 +
      for (keyFrm=1; keyFrm<numKeyFr && fNew>=sprite.keyFrames[keyFrm]; keyFrm++);
 +
      for (j=0; j<sprite.values.length; j++) {
 +
        props = sprite.values[j];
 +
        if (numKeyFr == props.length) propNum = keyFrm-1 //keyframes only
 +
        else propNum = Math.min(Math.max(0,fNew-firstKeyFr),props.length-1); //or keep in legal range
 +
        if (sprite.obj != null) {
 +
          if (props.prop2 == null) sprite.obj[props.prop] = props[propNum];
 +
          else        sprite.obj[props.prop2][props.prop] = props[propNum];
 +
      } }
 +
    } else if (sprite.charAt(0)=='b' && fNew == sprite.frame) eval(sprite.value);
 +
  }
 +
  tmLn.curFrame = fNew;
 +
  if (tmLn.ID == 0) eval('MM_timelinePlay(tmLnName)');
 +
}
  
 +
function MM_initTimelines() { //v4.0
 +
    //MM_initTimelines() Copyright 1997 Macromedia, Inc. All rights reserved.
 +
    var ns = navigator.appName == "Netscape";
 +
    var ns4 = (ns && parseInt(navigator.appVersion) == 4);
 +
    var ns5 = (ns && parseInt(navigator.appVersion) > 4);
 +
    var macIE5 = (navigator.platform ? (navigator.platform == "MacPPC") : false) && (navigator.appName == "Microsoft Internet Explorer") && (parseInt(navigator.appVersion) >= 4);
 +
    document.MM_Time = new Array(3);
 +
    document.MM_Time[0] = new Array(11);
 +
    document.MM_Time["Timeline1"] = document.MM_Time[0];
 +
    document.MM_Time[0].MM_Name = "Timeline1";
 +
    document.MM_Time[0].fps = 15;
 +
    document.MM_Time[0][0] = new String("sprite");
 +
    document.MM_Time[0][0].slot = 1;
 +
    if (ns4)
 +
        document.MM_Time[0][0].obj = document["Layer1"];
 +
    else if (ns5)
 +
        document.MM_Time[0][0].obj = document.getElementById("Layer1");
 +
    else
 +
        document.MM_Time[0][0].obj = document.all ? document.all["Layer1"] : null;
 +
    document.MM_Time[0][0].keyFrames = new Array(1, 2, 3);
 +
    document.MM_Time[0][0].values = new Array(3);
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][0].values[0] = new Array("0px", "0px", "0px");
 +
    else
 +
        document.MM_Time[0][0].values[0] = new Array(0,0,0);
 +
    document.MM_Time[0][0].values[0].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][0].values[1] = new Array("0px", "0px", "0px");
 +
    else
 +
        document.MM_Time[0][0].values[1] = new Array(0,0,0);
 +
    document.MM_Time[0][0].values[1].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[0][0].values[0].prop2 = "style";
 +
        document.MM_Time[0][0].values[1].prop2 = "style";
 +
    }
 +
    document.MM_Time[0][0].values[2] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[0][0].values[2].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][0].values[2].prop2 = "style";
 +
    document.MM_Time[0][1] = new String("sprite");
 +
    document.MM_Time[0][1].slot = 2;
 +
    if (ns4)
 +
        document.MM_Time[0][1].obj = document["Layer2"];
 +
    else if (ns5)
 +
        document.MM_Time[0][1].obj = document.getElementById("Layer2");
 +
    else
 +
        document.MM_Time[0][1].obj = document.all ? document.all["Layer2"] : null;
 +
    document.MM_Time[0][1].keyFrames = new Array(1, 3, 14);
 +
    document.MM_Time[0][1].values = new Array(1);
 +
    document.MM_Time[0][1].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[0][1].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][1].values[0].prop2 = "style";
 +
    document.MM_Time[0][2] = new String("sprite");
 +
    document.MM_Time[0][2].slot = 3;
 +
    if (ns4)
 +
        document.MM_Time[0][2].obj = document["Layer3"];
 +
    else if (ns5)
 +
        document.MM_Time[0][2].obj = document.getElementById("Layer3");
 +
    else
 +
        document.MM_Time[0][2].obj = document.all ? document.all["Layer3"] : null;
 +
    document.MM_Time[0][2].keyFrames = new Array(1, 3, 14, 21);
 +
    document.MM_Time[0][2].values = new Array(4);
 +
    document.MM_Time[0][2].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[0][2].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][2].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][2].values[1] = new Array("54px", "54px", "54px", "63px", "74px", "87px", "100px", "116px", "134px", "150px", "163px", "176px", "187px", "196px", "196px", "196px", "196px", "196px", "196px", "196px", "196px");
 +
    else
 +
        document.MM_Time[0][2].values[1] = new Array(54,54,54,63,74,87,100,116,134,150,163,176,187,196,196,196,196,196,196,196,196);
 +
    document.MM_Time[0][2].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][2].values[2] = new Array("24px", "24px", "24px", "24px", "23px", "22px", "22px", "21px", "20px", "19px", "19px", "18px", "17px", "17px", "17px", "17px", "17px", "17px", "17px", "17px", "17px");
 +
    else
 +
        document.MM_Time[0][2].values[2] = new Array(24,24,24,24,23,22,22,21,20,19,19,18,17,17,17,17,17,17,17,17,17);
 +
    document.MM_Time[0][2].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[0][2].values[1].prop2 = "style";
 +
        document.MM_Time[0][2].values[2].prop2 = "style";
 +
    }
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][2].values[3] = new Array("0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "5px", "11px", "17px", "22px", "28px", "34px", "40px");
 +
    else
 +
        document.MM_Time[0][2].values[3] = new Array(0,0,0,0,0,0,0,0,0,0,0,0,0,0,5,11,17,22,28,34,40);
 +
    document.MM_Time[0][2].values[3].prop = "height";
 +
    if (!ns4)
 +
        document.MM_Time[0][2].values[3].prop2 = "style";
 +
    document.MM_Time[0][3] = new String("sprite");
 +
    document.MM_Time[0][3].slot = 4;
 +
    if (ns4)
 +
        document.MM_Time[0][3].obj = document["Layer5"];
 +
    else if (ns5)
 +
        document.MM_Time[0][3].obj = document.getElementById("Layer5");
 +
    else
 +
        document.MM_Time[0][3].obj = document.all ? document.all["Layer5"] : null;
 +
    document.MM_Time[0][3].keyFrames = new Array(13, 14, 25);
 +
    document.MM_Time[0][3].values = new Array(5);
 +
    document.MM_Time[0][3].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[0][3].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][3].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][3].values[1] = new Array("215px", "215px", "212px", "208px", "203px", "199px", "193px", "187px", "181px", "174px", "168px", "161px", "155px");
 +
    else
 +
        document.MM_Time[0][3].values[1] = new Array(215,215,212,208,203,199,193,187,181,174,168,161,155);
 +
    document.MM_Time[0][3].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][3].values[2] = new Array("16px", "16px", "25px", "34px", "45px", "57px", "70px", "85px", "102px", "118px", "134px", "151px", "167px");
 +
    else
 +
        document.MM_Time[0][3].values[2] = new Array(16,16,25,34,45,57,70,85,102,118,134,151,167);
 +
    document.MM_Time[0][3].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[0][3].values[1].prop2 = "style";
 +
        document.MM_Time[0][3].values[2].prop2 = "style";
 +
    }
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][3].values[3] = new Array("0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px");
 +
    else
 +
        document.MM_Time[0][3].values[3] = new Array(0,0,0,0,0,0,0,0,0,0,0,0,0);
 +
    document.MM_Time[0][3].values[3].prop = "height";
 +
    if (!ns4)
 +
        document.MM_Time[0][3].values[3].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][3].values[4] = new Array("30px", "30px", "30px", "30px", "30px", "30px", "30px", "30px", "30px", "30px", "30px", "30px", "30px");
 +
    else
 +
        document.MM_Time[0][3].values[4] = new Array(30,30,30,30,30,30,30,30,30,30,30,30,30);
 +
    document.MM_Time[0][3].values[4].prop = "width";
 +
    if (!ns4)
 +
        document.MM_Time[0][3].values[4].prop2 = "style";
 +
    document.MM_Time[0][4] = new String("sprite");
 +
    document.MM_Time[0][4].slot = 5;
 +
    if (ns4)
 +
        document.MM_Time[0][4].obj = document["Layer4"];
 +
    else if (ns5)
 +
        document.MM_Time[0][4].obj = document.getElementById("Layer4");
 +
    else
 +
        document.MM_Time[0][4].obj = document.all ? document.all["Layer4"] : null;
 +
    document.MM_Time[0][4].keyFrames = new Array(24, 25, 30, 40);
 +
    document.MM_Time[0][4].values = new Array(3);
 +
    document.MM_Time[0][4].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[0][4].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][4].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][4].values[1] = new Array("125px", "125px", "125px", "125px", "125px", "125px", "125px", "135px", "146px", "158px", "172px", "188px", "206px", "224px", "242px", "261px", "279px");
 +
    else
 +
        document.MM_Time[0][4].values[1] = new Array(125,125,125,125,125,125,125,135,146,158,172,188,206,224,242,261,279);
 +
    document.MM_Time[0][4].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][4].values[2] = new Array("145px", "145px", "145px", "145px", "145px", "145px", "145px", "138px", "131px", "122px", "113px", "102px", "90px", "77px", "65px", "52px", "40px");
 +
    else
 +
        document.MM_Time[0][4].values[2] = new Array(145,145,145,145,145,145,145,138,131,122,113,102,90,77,65,52,40);
 +
    document.MM_Time[0][4].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[0][4].values[1].prop2 = "style";
 +
        document.MM_Time[0][4].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[0][5] = new String("sprite");
 +
    document.MM_Time[0][5].slot = 6;
 +
    if (ns4)
 +
        document.MM_Time[0][5].obj = document["Layer6"];
 +
    else if (ns5)
 +
        document.MM_Time[0][5].obj = document.getElementById("Layer6");
 +
    else
 +
        document.MM_Time[0][5].obj = document.all ? document.all["Layer6"] : null;
 +
    document.MM_Time[0][5].keyFrames = new Array(29, 30, 40);
 +
    document.MM_Time[0][5].values = new Array(1);
 +
    document.MM_Time[0][5].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[0][5].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][5].values[0].prop2 = "style";
 +
    document.MM_Time[0][6] = new String("sprite");
 +
    document.MM_Time[0][6].slot = 7;
 +
    if (ns4)
 +
        document.MM_Time[0][6].obj = document["Layer7"];
 +
    else if (ns5)
 +
        document.MM_Time[0][6].obj = document.getElementById("Layer7");
 +
    else
 +
        document.MM_Time[0][6].obj = document.all ? document.all["Layer7"] : null;
 +
    document.MM_Time[0][6].keyFrames = new Array(39, 40, 53, 57);
 +
    document.MM_Time[0][6].values = new Array(3);
 +
    document.MM_Time[0][6].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[0][6].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][6].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][6].values[1] = new Array("352px", "352px", "353px", "354px", "355px", "356px", "357px", "359px", "360px", "362px", "363px", "364px", "365px", "366px", "367px", "367px", "367px", "367px", "367px");
 +
    else
 +
        document.MM_Time[0][6].values[1] = new Array(352,352,353,354,355,356,357,359,360,362,363,364,365,366,367,367,367,367,367);
 +
    document.MM_Time[0][6].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[0][6].values[2] = new Array("70px", "70px", "68px", "67px", "65px", "63px", "61px", "58px", "55px", "52px", "50px", "48px", "46px", "45px", "43px", "43px", "43px", "43px", "43px");
 +
    else
 +
        document.MM_Time[0][6].values[2] = new Array(70,70,68,67,65,63,61,58,55,52,50,48,46,45,43,43,43,43,43);
 +
    document.MM_Time[0][6].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[0][6].values[1].prop2 = "style";
 +
        document.MM_Time[0][6].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[0][7] = new String("sprite");
 +
    document.MM_Time[0][7].slot = 8;
 +
    if (ns4)
 +
        document.MM_Time[0][7].obj = document["Layer8"];
 +
    else if (ns5)
 +
        document.MM_Time[0][7].obj = document.getElementById("Layer8");
 +
    else
 +
        document.MM_Time[0][7].obj = document.all ? document.all["Layer8"] : null;
 +
    document.MM_Time[0][7].keyFrames = new Array(39, 43, 51, 57);
 +
    document.MM_Time[0][7].values = new Array(1);
 +
    document.MM_Time[0][7].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[0][7].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][7].values[0].prop2 = "style";
 +
    document.MM_Time[0][8] = new String("behavior");
 +
    document.MM_Time[0][8].frame = 60;
 +
    document.MM_Time[0][8].value = "MM_timelineGoto('Timeline1','1');MM_timelineStop('Timeline1')";
 +
    document.MM_Time[0][9] = new String("sprite");
 +
    document.MM_Time[0][9].slot = 9;
 +
    if (ns4)
 +
        document.MM_Time[0][9].obj = document["Layer-all1"];
 +
    else if (ns5)
 +
        document.MM_Time[0][9].obj = document.getElementById("Layer-all1");
 +
    else
 +
        document.MM_Time[0][9].obj = document.all ? document.all["Layer-all1"] : null;
 +
    document.MM_Time[0][9].keyFrames = new Array(57, 60);
 +
    document.MM_Time[0][9].values = new Array(1);
 +
    document.MM_Time[0][9].values[0] = new Array("inherit","inherit");
 +
    document.MM_Time[0][9].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][9].values[0].prop2 = "style";
 +
    document.MM_Time[0][10] = new String("sprite");
 +
    document.MM_Time[0][10].slot = 9;
 +
    if (ns4)
 +
        document.MM_Time[0][10].obj = document["Layer-all1"];
 +
    else if (ns5)
 +
        document.MM_Time[0][10].obj = document.getElementById("Layer-all1");
 +
    else
 +
        document.MM_Time[0][10].obj = document.all ? document.all["Layer-all1"] : null;
 +
    document.MM_Time[0][10].keyFrames = new Array(1, 2);
 +
    document.MM_Time[0][10].values = new Array(1);
 +
    document.MM_Time[0][10].values[0] = new Array("visible","hidden");
 +
    document.MM_Time[0][10].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[0][10].values[0].prop2 = "style";
 +
    document.MM_Time[0].lastFrame = 60;
 +
    document.MM_Time[1] = new Array(11);
 +
    document.MM_Time["Timeline2"] = document.MM_Time[1];
 +
    document.MM_Time[1].MM_Name = "Timeline2";
 +
    document.MM_Time[1].fps = 15;
 +
    document.MM_Time[1][0] = new String("sprite");
 +
    document.MM_Time[1][0].slot = 1;
 +
    if (ns4)
 +
        document.MM_Time[1][0].obj = document["Layer11"];
 +
    else if (ns5)
 +
        document.MM_Time[1][0].obj = document.getElementById("Layer11");
 +
    else
 +
        document.MM_Time[1][0].obj = document.all ? document.all["Layer11"] : null;
 +
    document.MM_Time[1][0].keyFrames = new Array(1, 2, 12, 13);
 +
    document.MM_Time[1][0].values = new Array(1);
 +
    document.MM_Time[1][0].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[1][0].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][0].values[0].prop2 = "style";
 +
    document.MM_Time[1][1] = new String("sprite");
 +
    document.MM_Time[1][1].slot = 2;
 +
    if (ns4)
 +
        document.MM_Time[1][1].obj = document["Layer12"];
 +
    else if (ns5)
 +
        document.MM_Time[1][1].obj = document.getElementById("Layer12");
 +
    else
 +
        document.MM_Time[1][1].obj = document.all ? document.all["Layer12"] : null;
 +
    document.MM_Time[1][1].keyFrames = new Array(1, 3, 12, 13);
 +
    document.MM_Time[1][1].values = new Array(4);
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][1].values[0] = new Array("26px", "26px", "26px", "26px", "27px", "27px", "28px", "29px", "29px", "30px", "30px", "31px", "32px");
 +
    else
 +
        document.MM_Time[1][1].values[0] = new Array(26,26,26,26,27,27,28,29,29,30,30,31,32);
 +
    document.MM_Time[1][1].values[0].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][1].values[1] = new Array("8px", "8px", "8px", "14px", "20px", "28px", "37px", "47px", "57px", "66px", "74px", "81px", "68px");
 +
    else
 +
        document.MM_Time[1][1].values[1] = new Array(8,8,8,14,20,28,37,47,57,66,74,81,68);
 +
    document.MM_Time[1][1].values[1].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[1][1].values[0].prop2 = "style";
 +
        document.MM_Time[1][1].values[1].prop2 = "style";
 +
    }
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][1].values[2] = new Array("61px", "30px", "0px", "6px", "12px", "18px", "24px", "30px", "36px", "42px", "48px", "54px", "61px");
 +
    else
 +
        document.MM_Time[1][1].values[2] = new Array(61,30,0,6,12,18,24,30,36,42,48,54,61);
 +
    document.MM_Time[1][1].values[2].prop = "height";
 +
    if (!ns4)
 +
        document.MM_Time[1][1].values[2].prop2 = "style";
 +
    document.MM_Time[1][1].values[3] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[1][1].values[3].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][1].values[3].prop2 = "style";
 +
    document.MM_Time[1][2] = new String("sprite");
 +
    document.MM_Time[1][2].slot = 3;
 +
    if (ns4)
 +
        document.MM_Time[1][2].obj = document["Layer13"];
 +
    else if (ns5)
 +
        document.MM_Time[1][2].obj = document.getElementById("Layer13");
 +
    else
 +
        document.MM_Time[1][2].obj = document.all ? document.all["Layer13"] : null;
 +
    document.MM_Time[1][2].keyFrames = new Array(1, 4, 12, 13);
 +
    document.MM_Time[1][2].values = new Array(3);
 +
    document.MM_Time[1][2].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[1][2].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][2].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][2].values[1] = new Array("103px", "103px", "103px", "103px", "100px", "97px", "93px", "89px", "84px", "80px", "76px", "73px", "82px");
 +
    else
 +
        document.MM_Time[1][2].values[1] = new Array(103,103,103,103,100,97,93,89,84,80,76,73,82);
 +
    document.MM_Time[1][2].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][2].values[2] = new Array("7px", "7px", "7px", "7px", "15px", "24px", "35px", "48px", "61px", "74px", "84px", "94px", "81px");
 +
    else
 +
        document.MM_Time[1][2].values[2] = new Array(7,7,7,7,15,24,35,48,61,74,84,94,81);
 +
    document.MM_Time[1][2].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[1][2].values[1].prop2 = "style";
 +
        document.MM_Time[1][2].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[1][3] = new String("sprite");
 +
    document.MM_Time[1][3].slot = 4;
 +
    if (ns4)
 +
        document.MM_Time[1][3].obj = document["Layer14"];
 +
    else if (ns5)
 +
        document.MM_Time[1][3].obj = document.getElementById("Layer14");
 +
    else
 +
        document.MM_Time[1][3].obj = document.all ? document.all["Layer14"] : null;
 +
    document.MM_Time[1][3].keyFrames = new Array(12, 13, 25);
 +
    document.MM_Time[1][3].values = new Array(1);
 +
    document.MM_Time[1][3].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[1][3].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][3].values[0].prop2 = "style";
 +
    document.MM_Time[1][4] = new String("sprite");
 +
    document.MM_Time[1][4].slot = 5;
 +
    if (ns4)
 +
        document.MM_Time[1][4].obj = document["Layer15"];
 +
    else if (ns5)
 +
        document.MM_Time[1][4].obj = document.getElementById("Layer15");
 +
    else
 +
        document.MM_Time[1][4].obj = document.all ? document.all["Layer15"] : null;
 +
    document.MM_Time[1][4].keyFrames = new Array(13, 14, 25);
 +
    document.MM_Time[1][4].values = new Array(3);
 +
    document.MM_Time[1][4].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[1][4].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][4].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][4].values[1] = new Array("247px", "247px", "241px", "235px", "228px", "220px", "211px", "201px", "191px", "180px", "169px", "159px", "148px");
 +
    else
 +
        document.MM_Time[1][4].values[1] = new Array(247,247,241,235,228,220,211,201,191,180,169,159,148);
 +
    document.MM_Time[1][4].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][4].values[2] = new Array("5px", "5px", "6px", "8px", "10px", "12px", "14px", "16px", "19px", "21px", "24px", "26px", "29px");
 +
    else
 +
        document.MM_Time[1][4].values[2] = new Array(5,5,6,8,10,12,14,16,19,21,24,26,29);
 +
    document.MM_Time[1][4].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[1][4].values[1].prop2 = "style";
 +
        document.MM_Time[1][4].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[1][5] = new String("sprite");
 +
    document.MM_Time[1][5].slot = 6;
 +
    if (ns4)
 +
        document.MM_Time[1][5].obj = document["Layer16"];
 +
    else if (ns5)
 +
        document.MM_Time[1][5].obj = document.getElementById("Layer16");
 +
    else
 +
        document.MM_Time[1][5].obj = document.all ? document.all["Layer16"] : null;
 +
    document.MM_Time[1][5].keyFrames = new Array(24, 25, 38);
 +
    document.MM_Time[1][5].values = new Array(3);
 +
    document.MM_Time[1][5].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[1][5].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][5].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][5].values[1] = new Array("-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px", "-5px");
 +
    else
 +
        document.MM_Time[1][5].values[1] = new Array(-5,-5,-5,-5,-5,-5,-5,-5,-5,-5,-5,-5,-5,-5,-5);
 +
    document.MM_Time[1][5].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][5].values[2] = new Array("55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px", "55px");
 +
    else
 +
        document.MM_Time[1][5].values[2] = new Array(55,55,55,55,55,55,55,55,55,55,55,55,55,55,55);
 +
    document.MM_Time[1][5].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[1][5].values[1].prop2 = "style";
 +
        document.MM_Time[1][5].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[1][6] = new String("sprite");
 +
    document.MM_Time[1][6].slot = 7;
 +
    if (ns4)
 +
        document.MM_Time[1][6].obj = document["Layer17"];
 +
    else if (ns5)
 +
        document.MM_Time[1][6].obj = document.getElementById("Layer17");
 +
    else
 +
        document.MM_Time[1][6].obj = document.all ? document.all["Layer17"] : null;
 +
    document.MM_Time[1][6].keyFrames = new Array(25, 26, 38);
 +
    document.MM_Time[1][6].values = new Array(3);
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][6].values[0] = new Array("245px", "245px", "241px", "237px", "232px", "226px", "220px", "214px", "206px", "199px", "191px", "183px", "176px", "168px");
 +
    else
 +
        document.MM_Time[1][6].values[0] = new Array(245,245,241,237,232,226,220,214,206,199,191,183,176,168);
 +
    document.MM_Time[1][6].values[0].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[1][6].values[1] = new Array("196px", "196px", "194px", "192px", "189px", "187px", "184px", "180px", "177px", "173px", "169px", "166px", "162px", "158px");
 +
    else
 +
        document.MM_Time[1][6].values[1] = new Array(196,196,194,192,189,187,184,180,177,173,169,166,162,158);
 +
    document.MM_Time[1][6].values[1].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[1][6].values[0].prop2 = "style";
 +
        document.MM_Time[1][6].values[1].prop2 = "style";
 +
    }
 +
    document.MM_Time[1][6].values[2] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[1][6].values[2].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][6].values[2].prop2 = "style";
 +
    document.MM_Time[1][7] = new String("sprite");
 +
    document.MM_Time[1][7].slot = 8;
 +
    if (ns4)
 +
        document.MM_Time[1][7].obj = document["Layer18"];
 +
    else if (ns5)
 +
        document.MM_Time[1][7].obj = document.getElementById("Layer18");
 +
    else
 +
        document.MM_Time[1][7].obj = document.all ? document.all["Layer18"] : null;
 +
    document.MM_Time[1][7].keyFrames = new Array(37, 38, 46);
 +
    document.MM_Time[1][7].values = new Array(1);
 +
    document.MM_Time[1][7].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[1][7].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][7].values[0].prop2 = "style";
 +
    document.MM_Time[1][8] = new String("sprite");
 +
    document.MM_Time[1][8].slot = 9;
 +
    if (ns4)
 +
        document.MM_Time[1][8].obj = document["Layer-all2"];
 +
    else if (ns5)
 +
        document.MM_Time[1][8].obj = document.getElementById("Layer-all2");
 +
    else
 +
        document.MM_Time[1][8].obj = document.all ? document.all["Layer-all2"] : null;
 +
    document.MM_Time[1][8].keyFrames = new Array(46, 47);
 +
    document.MM_Time[1][8].values = new Array(1);
 +
    document.MM_Time[1][8].values[0] = new Array("visible","inherit");
 +
    document.MM_Time[1][8].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][8].values[0].prop2 = "style";
 +
    document.MM_Time[1][9] = new String("sprite");
 +
    document.MM_Time[1][9].slot = 9;
 +
    if (ns4)
 +
        document.MM_Time[1][9].obj = document["Layer-all2"];
 +
    else if (ns5)
 +
        document.MM_Time[1][9].obj = document.getElementById("Layer-all2");
 +
    else
 +
        document.MM_Time[1][9].obj = document.all ? document.all["Layer-all2"] : null;
 +
    document.MM_Time[1][9].keyFrames = new Array(2, 3);
 +
    document.MM_Time[1][9].values = new Array(1);
 +
    document.MM_Time[1][9].values[0] = new Array("visible","hidden");
 +
    document.MM_Time[1][9].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[1][9].values[0].prop2 = "style";
 +
    document.MM_Time[1][10] = new String("behavior");
 +
    document.MM_Time[1][10].frame = 48;
 +
    document.MM_Time[1][10].value = "MM_timelineGoto('Timeline2','1');MM_timelineStop('Timeline2')";
 +
    document.MM_Time[1].lastFrame = 48;
 +
    document.MM_Time = new Array(1);
 +
    document.MM_Time[2] = new Array(9);
 +
    document.MM_Time["Timeline3"] = document.MM_Time[2];
 +
    document.MM_Time[2].MM_Name = "Timeline3";
 +
    document.MM_Time[2].fps = 15;
 +
    document.MM_Time[2][0] = new String("sprite");
 +
    document.MM_Time[2][0].slot = 1;
 +
    if (ns4)
 +
        document.MM_Time[2][0].obj = document["Layer21"];
 +
    else if (ns5)
 +
        document.MM_Time[2][0].obj = document.getElementById("Layer21");
 +
    else
 +
        document.MM_Time[2][0].obj = document.all ? document.all["Layer21"] : null;
 +
    document.MM_Time[2][0].keyFrames = new Array(1, 2, 14, 15);
 +
    document.MM_Time[2][0].values = new Array(3);
 +
    document.MM_Time[2][0].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[2][0].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][0].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][0].values[1] = new Array("0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px", "0px");
 +
    else
 +
        document.MM_Time[2][0].values[1] = new Array(0,0,0,0,0,0,0,0,0,0,0,0,0,0,0);
 +
    document.MM_Time[2][0].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][0].values[2] = new Array("120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px", "120px");
 +
    else
 +
        document.MM_Time[2][0].values[2] = new Array(120,120,120,120,120,120,120,120,120,120,120,120,120,120,120);
 +
    document.MM_Time[2][0].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[2][0].values[1].prop2 = "style";
 +
        document.MM_Time[2][0].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[2][1] = new String("sprite");
 +
    document.MM_Time[2][1].slot = 2;
 +
    if (ns4)
 +
        document.MM_Time[2][1].obj = document["Layer22"];
 +
    else if (ns5)
 +
        document.MM_Time[2][1].obj = document.getElementById("Layer22");
 +
    else
 +
        document.MM_Time[2][1].obj = document.all ? document.all["Layer22"] : null;
 +
    document.MM_Time[2][1].keyFrames = new Array(1, 3, 14, 15);
 +
    document.MM_Time[2][1].values = new Array(3);
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][1].values[0] = new Array("30px", "30px", "30px", "30px", "30px", "30px", "29px", "29px", "29px", "29px", "28px", "28px", "28px", "28px", "30px");
 +
    else
 +
        document.MM_Time[2][1].values[0] = new Array(30,30,30,30,30,30,29,29,29,29,28,28,28,28,30);
 +
    document.MM_Time[2][1].values[0].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][1].values[1] = new Array("19px", "19px", "19px", "27px", "37px", "48px", "60px", "74px", "89px", "104px", "117px", "129px", "139px", "149px", "149px");
 +
    else
 +
        document.MM_Time[2][1].values[1] = new Array(19,19,19,27,37,48,60,74,89,104,117,129,139,149,149);
 +
    document.MM_Time[2][1].values[1].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[2][1].values[0].prop2 = "style";
 +
        document.MM_Time[2][1].values[1].prop2 = "style";
 +
    }
 +
    document.MM_Time[2][1].values[2] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[2][1].values[2].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][1].values[2].prop2 = "style";
 +
    document.MM_Time[2][2] = new String("sprite");
 +
    document.MM_Time[2][2].slot = 3;
 +
    if (ns4)
 +
        document.MM_Time[2][2].obj = document["Layer23"];
 +
    else if (ns5)
 +
        document.MM_Time[2][2].obj = document.getElementById("Layer23");
 +
    else
 +
        document.MM_Time[2][2].obj = document.all ? document.all["Layer23"] : null;
 +
    document.MM_Time[2][2].keyFrames = new Array(14, 15, 29);
 +
    document.MM_Time[2][2].values = new Array(1);
 +
    document.MM_Time[2][2].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[2][2].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][2].values[0].prop2 = "style";
 +
    document.MM_Time[2][3] = new String("sprite");
 +
    document.MM_Time[2][3].slot = 4;
 +
    if (ns4)
 +
        document.MM_Time[2][3].obj = document["Layer24"];
 +
    else if (ns5)
 +
        document.MM_Time[2][3].obj = document.getElementById("Layer24");
 +
    else
 +
        document.MM_Time[2][3].obj = document.all ? document.all["Layer24"] : null;
 +
    document.MM_Time[2][3].keyFrames = new Array(15, 16, 28, 29);
 +
    document.MM_Time[2][3].values = new Array(3);
 +
    document.MM_Time[2][3].values[0] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[2][3].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][3].values[0].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][3].values[1] = new Array("314px", "314px", "308px", "302px", "295px", "288px", "279px", "270px", "260px", "249px", "238px", "227px", "217px", "206px", "314px");
 +
    else
 +
        document.MM_Time[2][3].values[1] = new Array(314,314,308,302,295,288,279,270,260,249,238,227,217,206,314);
 +
    document.MM_Time[2][3].values[1].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][3].values[2] = new Array("14px", "14px", "18px", "22px", "27px", "32px", "38px", "44px", "51px", "58px", "65px", "73px", "80px", "87px", "14px");
 +
    else
 +
        document.MM_Time[2][3].values[2] = new Array(14,14,18,22,27,32,38,44,51,58,65,73,80,87,14);
 +
    document.MM_Time[2][3].values[2].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[2][3].values[1].prop2 = "style";
 +
        document.MM_Time[2][3].values[2].prop2 = "style";
 +
    }
 +
    document.MM_Time[2][4] = new String("sprite");
 +
    document.MM_Time[2][4].slot = 5;
 +
    if (ns4)
 +
        document.MM_Time[2][4].obj = document["Layer25"];
 +
    else if (ns5)
 +
        document.MM_Time[2][4].obj = document.getElementById("Layer25");
 +
    else
 +
        document.MM_Time[2][4].obj = document.all ? document.all["Layer25"] : null;
 +
    document.MM_Time[2][4].keyFrames = new Array(28, 29, 55);
 +
    document.MM_Time[2][4].values = new Array(1);
 +
    document.MM_Time[2][4].values[0] = new Array("hidden","visible","inherit");
 +
    document.MM_Time[2][4].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][4].values[0].prop2 = "style";
 +
    document.MM_Time[2][5] = new String("sprite");
 +
    document.MM_Time[2][5].slot = 6;
 +
    if (ns4)
 +
        document.MM_Time[2][5].obj = document["Layer26"];
 +
    else if (ns5)
 +
        document.MM_Time[2][5].obj = document.getElementById("Layer26");
 +
    else
 +
        document.MM_Time[2][5].obj = document.all ? document.all["Layer26"] : null;
 +
    document.MM_Time[2][5].keyFrames = new Array(29, 31, 55);
 +
    document.MM_Time[2][5].values = new Array(1);
 +
    document.MM_Time[2][5].values[0] = new Array("hidden","visible","hidden");
 +
    document.MM_Time[2][5].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][5].values[0].prop2 = "style";
 +
    document.MM_Time[2][6] = new String("sprite");
 +
    document.MM_Time[2][6].slot = 7;
 +
    if (ns4)
 +
        document.MM_Time[2][6].obj = document["Layer27"];
 +
    else if (ns5)
 +
        document.MM_Time[2][6].obj = document.getElementById("Layer27");
 +
    else
 +
        document.MM_Time[2][6].obj = document.all ? document.all["Layer27"] : null;
 +
    document.MM_Time[2][6].keyFrames = new Array(29, 33, 46, 48);
 +
    document.MM_Time[2][6].values = new Array(4);
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][6].values[0] = new Array("370px", "370px", "370px", "370px", "370px", "360px", "349px", "337px", "323px", "307px", "290px", "272px", "255px", "239px", "225px", "213px", "202px", "192px", "192px", "192px");
 +
    else
 +
        document.MM_Time[2][6].values[0] = new Array(370,370,370,370,370,360,349,337,323,307,290,272,255,239,225,213,202,192,192,192);
 +
    document.MM_Time[2][6].values[0].prop = "left";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][6].values[1] = new Array("97px", "97px", "97px", "97px", "97px", "99px", "102px", "104px", "107px", "111px", "114px", "119px", "122px", "126px", "129px", "131px", "134px", "136px", "136px", "136px");
 +
    else
 +
        document.MM_Time[2][6].values[1] = new Array(97,97,97,97,97,99,102,104,107,111,114,119,122,126,129,131,134,136,136,136);
 +
    document.MM_Time[2][6].values[1].prop = "top";
 +
    if (!ns4) {
 +
        document.MM_Time[2][6].values[0].prop2 = "style";
 +
        document.MM_Time[2][6].values[1].prop2 = "style";
 +
    }
 +
    document.MM_Time[2][6].values[2] = new Array("hidden","visible","visible","hidden");
 +
    document.MM_Time[2][6].values[2].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][6].values[2].prop2 = "style";
 +
    if (ns5 || macIE5)
 +
        document.MM_Time[2][6].values[3] = new Array("103px", "103px", "103px", "103px", "103px", "95px", "87px", "79px", "71px", "63px", "55px", "47px", "39px", "31px", "23px", "15px", "7px", "0px", "51px", "103px");
 +
    else
 +
        document.MM_Time[2][6].values[3] = new Array(103,103,103,103,103,95,87,79,71,63,55,47,39,31,23,15,7,0,51,103);
 +
    document.MM_Time[2][6].values[3].prop = "height";
 +
    if (!ns4)
 +
        document.MM_Time[2][6].values[3].prop2 = "style";
 +
    document.MM_Time[2][7] = new String("sprite");
 +
    document.MM_Time[2][7].slot = 8;
 +
    if (ns4)
 +
        document.MM_Time[2][7].obj = document["Layer28"];
 +
    else if (ns5)
 +
        document.MM_Time[2][7].obj = document.getElementById("Layer28");
 +
    else
 +
        document.MM_Time[2][7].obj = document.all ? document.all["Layer28"] : null;
 +
    document.MM_Time[2][7].keyFrames = new Array(46, 47, 55);
 +
    document.MM_Time[2][7].values = new Array(1);
 +
    document.MM_Time[2][7].values[0] = new Array("hidden","visible","inherit");
 +
    document.MM_Time[2][7].values[0].prop = "visibility";
 +
    if (!ns4)
 +
        document.MM_Time[2][7].values[0].prop2 = "style";
 +
    document.MM_Time[2][8] = new String("behavior");
 +
    document.MM_Time[2][8].frame = 56;
 +
    document.MM_Time[2][8].value = "MM_timelineGoto('Timeline3','1')";
 +
    document.MM_Time[2].lastFrame = 56;
 +
    for (i=0; i<document.MM_Time.length; i++) {
 +
        document.MM_Time[i].ID = null;
 +
        document.MM_Time[i].curFrame = 0;
 +
        document.MM_Time[i].delay = 1000/document.MM_Time[i].fps;
 +
    }
 +
}
 +
function MM_effectGrowShrink(targetElement, duration, from, to, toggle, referHeight, growFromCenter)
 +
{
 +
Spry.Effect.DoGrow(targetElement, {duration: duration, from: from, to: to, toggle: toggle, referHeight: referHeight, growCenter: growFromCenter});
 +
}
 +
function MM_effectBlind(targetElement, duration, from, to, toggle)
 +
{
 +
Spry.Effect.DoBlind(targetElement, {duration: duration, from: from, to: to, toggle: toggle});
 +
}
 +
function MM_effectAppearFade(targetElement, duration, from, to, toggle)
 +
{
 +
Spry.Effect.DoFade(targetElement, {duration: duration, from: from, to: to, toggle: toggle});
 +
}
 +
function MM_preloadImages() { //v3.0
 +
  var d=document; if(d.images){ if(!d.MM_p) d.MM_p=new Array();
 +
    var i,j=d.MM_p.length,a=MM_preloadImages.arguments; for(i=0; i<a.length; i++)
 +
    if (a[i].indexOf("#")!=0){ d.MM_p[j]=new Image; d.MM_p[j++].src=a[i];}}
 +
}
 +
</script>
 +
</head>
  
−
 
+
<body>
−
<div class="column two_thirds_size">
+
<div id="d">
−
<h3>What should this page contain?</h3>
+
<img src="https://static.igem.org/mediawiki/2018/f/fe/T--Nanjing-China--signal-1.jpg" width="100%" onclick="MM_effectBlind('HOME', 1000, '0%', '100%', true)" />
−
<ul>
+
<div align="center">
−
<li>Explanation of the engineering principles your team used in your design</li>
+
<div id="HOME">
−
<li>Discussion of the design iterations your team went through</li>
+
<div class="sub">
−
<li>Experimental plan to test your designs</li>
+
      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li></ul></div>
−
</ul>
+
<div class="sub">
−
 
+
      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></ul></li></div>
 +
      <ul>
 +
    <li><a href="#intro">introduction</a></li>
 +
  <li><a href="#gas">gas</a></li>
 +
    <li><a href="#how">how</a></li>
 +
  </ul>
 
</div>
 
</div>
−
 
−
<div class="column third_size">
 
−
<div class="highlight decoration_A_full">
 
−
<h3>Inspiration</h3>
 
−
<ul>
 
−
<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
 
−
<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
 
−
<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
 
−
</ul>
 
 
</div>
 
</div>
 
</div>
 
</div>
 +
<div class="container" align="center">
 +
  <div id="menu">
 +
    <ul>
 +
  <li><a href="https://2018.igem.org/Team:Nanjing-China">HOME</a>
 +
    <ul>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
 +
</ul>
 +
    </li>
 +
    <li><a href="https://2017.igem.org/Team:Nanjing-China/Parts">PARTS</a>
 +
        <ul>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improved_Parts">Improved</a></li>
 +
</ul>
 +
            </li>
 +
    <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">JUDGE</a>
 +
    <ul>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Sliver">Sliver</a></li>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Gold">Gold</a></li>
 +
</ul>
 +
      </li>
 +
    <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">PROJECT</a>
 +
    <ul>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Improvement">Improvement</a></li>
 +
</ul>
 +
        </li>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">TEAM</a>
 +
    <ul>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Introduction</a></li>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
 +
</ul>
 +
    </li>
 +
    <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">OTHERS</a>
 +
    <ul>
 +
        <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li>
 +
                <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
 +
            <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></li>
 +
</ul>
 +
    </li>
 +
      </ul>
 +
  </div>
 +
  <div class="header"><img src="https://static.igem.org/mediawiki/2018/f/f3/T--Nanjing-China--title-1-1.png" width="100%" >
 +
</div>
 +
    <div class="contain" align="left">
 +
    <div class="word">
 +
    <p>Liying Wang et. demonstrated a minimal nitrogen  fixation gene cluster from Paenibacillus sp.WLY 78 which was compried of Pnif  promoter and nine structural genes. Inspired by this study, we transferred this  gene cluster to E.coli to create engineered E.coli cells which are capable of  producing active nitrogenase. We achieved this by extracting the gene cluster  from Paenibacillus sp.WLY78, connecting it to plasmid Psb1C3 and transformed it  to E coli cells.In order to ensure the expression of this gene cluster in E  coli,first we verified the transcriptional activity of Pnif promoter in E coli  cells by conducting control experiments.In the test group,we replaced the  native T5 promoter on pQE80L vector with Pnif,connected it to Dronpa fluorescent  protein gene and transformed the new vectors to E.coli cells.In the control  group, pQE80L vectors with T5 promoter and Dronpa gene were transformed to E  coli cells. The comparable level of fluorescence intensity of the two groups  indicated that Pnif promoter is transcriptional active in E coli cells.</p></div>
 +
<div class="word">
 +
<div id="Layer1" style="visibility: hidden; left: 0; top: 0;"><img src="https://static.igem.org/mediawiki/2018/1/11/T--Nanjing-China--d-1-1.png" width="150" /></div>
 +
<div id="Layer2" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/d/d5/T--Nanjing-China--d-1-2.png" name="Image1" width="150" /></div>
 +
<div id="Layer3" style="visibility: hidden; left: 54px; top: 24px; height: 0px;"><img src="https://static.igem.org/mediawiki/2018/f/f7/T--Nanjing-China--d-1-3.png" width="50" /></div>
 +
<div id="Layer4" style="visibility: hidden; left: 125px; top: 145px;"><img src="https://static.igem.org/mediawiki/2018/e/e0/T--Nanjing-China--d-1-4.png" width="300" /></div>
 +
<div id="Layer5" style="visibility: hidden; left: 215px; top: 16px; height: 0px; width: 30;"><img src="https://static.igem.org/mediawiki/2018/8/83/T--Nanjing-China--d-1-5.png" width="30" /></div>
 +
<div id="Layer6" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/6/6b/T--Nanjing-China--d-1-6.png" width="70" /></div>
 +
<div id="Layer7" style="visibility: hidden; left: 352px; top: 70px;"><img src="https://static.igem.org/mediawiki/2018/8/85/T--Nanjing-China--d-1-7.png" name="Image2" width="70" /></div>
 +
<div id="Layer8" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/27/T--Nanjing-China--d-1-8.png" width="170" /></div>
 +
<div class="play" onclick="MM_timelinePlay('Timeline1')">play
 +
</div>
 +
<div class="stop" onclick="MM_timelineStop('Timeline1')">stop
 +
</div>
 +
<div id="Layer-all1" style="visibility: visible"><img src="https://static.igem.org/mediawiki/2018/a/a9/T--Nanjing-China--d-1-all.png" name="Image3" width="500" /></div>
 +
</div>
 +
<div class="word">
 +
<p>Simultaneously, in order to achieve in site synthesis of CdS nanocrystals on cell surfaces,we transformed the vector with opmA-PbrR gene to E.coli cells.This gene encodes opmA-PbrR protein complex,which can be fixed on cell surface by outer membrane protein (OMP) opmA.The function of PbrR protein is to adsorb Cd2+ in the environment and further form CdS nanocrystal on cell surface,a key component of this light-harvesting system. When this system is exposed by light, electrons from electron donor conduct transition while CdS nanocrystals on cell surfaces transfer high-energy electrons to Mo-Fe protein subunit of nitrogenase.Mo-Fe protein then  utilizes the energy from these electrons rather than ATP to reduce dinitrogen into ammonia. With the method mentioned above, we successfully constructed a whole-cell light-driven nitrogen fixation system.</p>
 +
</div>
 +
<div class="word">
 +
<div id="Layer11" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/27/T--Nanjing-China--d-1-8.png" width="150" /></div>
 +
<div id="Layer12" style="height: 61px; visibility: hidden; left: 26px; top: 8px;"><img src="https://static.igem.org/mediawiki/2018/8/85/T--Nanjing-China--d-1-7.png" width="60" /></div>
 +
<div id="Layer13" style="visibility: hidden; left: 103px; top: 7px;"><img src="https://static.igem.org/mediawiki/2018/a/a7/T--Nanjing-China--d-2-2.png" width="100" /></div>
 +
<div id="Layer14" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/0/0d/T--Nanjing-China--d-2-1.png" width="200" /></div>
 +
<div id="Layer15" style="visibility: hidden; left: 247px; top: 5px;"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--Nanjing-China--d-2-3.png" width="130" /></div>
 +
<div id="Layer16" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/26/T--Nanjing-China--d-2-4.png" name="Image1" width="202" /></div>
 +
<div id="Layer17" style="visibility: hidden; left: 245px; top: 196px;"><img src="https://static.igem.org/mediawiki/2018/0/03/T--Nanjing-China--d-2-5.png" width="130" /></div>
 +
<div id="Layer18" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/8/8a/T--Nanjing-China--d-2-6.png" width="200" /></div>
 +
<div id="Layer-all2" style="visibility: visible"><img src="https://static.igem.org/mediawiki/2018/2/29/T--Nanjing-China--d-2-all.png" width="500" /></div>
 +
<div class="play" onclick="MM_timelinePlay('Timeline2')">play</div>
 +
<div class="stop" onclick="MM_timelineStop('Timeline2')">stop</div>
  
−
 
+
</div>
−
 
+
<div class="word">
 +
<p>In later period of our study, in order to elevate the electron transfer efficiency, we introduced Ag2S to realize Ag2S CdS cocatalysis. We respectively introduced ompA-spy tag Ag oligopeptide complex and ompA-spychcatcher-PbrR complex to E.coli cells. In that way, two types of E coli cells--with Ag2S or CdS nanocrystals adsorbed on surfaces specifically bind with each other through covalent bonds between spytag and spycatcher. Eventually, nitrogen fixation efficiency of our system showed a remarkable increase</p>
 +
<img src="https://static.igem.org/mediawiki/2018/7/77/T--Nanjing-China--design-3.png"width="500" />
 +
</div>
 +
<div class="word">
 +
<div id="Layer21" style="visibility: hidden; left: 0px;"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--Nanjing-China--d-3-8.png" width="240" /></div>
 +
<div id="Layer22" style="visibility: hidden; top: 19px; left: 30px;"><img src="https://static.igem.org/mediawiki/2018/9/9e/T--Nanjing-China--d-3-7.png" width="170" /></div>
 +
<div id="Layer23" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/21/T--Nanjing-China--d-3-1.png" width="250" /></div>
 +
<div id="Layer24" style="visibility: hidden; left: 314px; top: 14px;"><img src="https://static.igem.org/mediawiki/2018/f/fd/T--Nanjing-China--d-3-2.png" width="60" /></div>
 +
<div id="Layer25" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/a/a8/T--Nanjing-China--d-3-3.png" width="260" /></div>
 +
<div id="Layer26" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/9/9d/T--Nanjing-China--d-3-4.png" width="211" /></div>
 +
<div id="Layer27" style="visibility: hidden; left: 370px; top: 97px; height: 103px;"><img src="https://static.igem.org/mediawiki/2018/2/28/T--Nanjing-China--d-3-6.png" width="100" /></div>
 +
<div id="Layer28" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--Nanjing-China--d-3-5.png" width="150" /></div>
 +
<div class="play" onclick="MM_timelinePlay('Timeline3')">play</div>
 +
<div class="stop" onclick="MM_timelineStop('Timeline3')">stop</div></div>
 +
</div>
 +
<div class="footer">
 +
    <div align="center">
 +
      <div class="f">
 +
        <div class="f-word"><p><strong><u>Address</u></strong></p>
 +
            <p>Life Science Department<br />
 +
              #163 Xianlin Blvd, Qixia District<br />
 +
              Nanjing University<br />
 +
              Nanjing, Jiangsu Province<br />
 +
              P.R. of China<br />
 +
              Zip: 210046
 +
            </p></div>
 +
          <div class="f-word">
 +
            <p><strong><u>Email</u></strong></p>
 +
            <p>nanjing_china@163.com</p>
 +
            <p><strong><u>Social media</u></strong></p>
 +
            <p>Twitter button: <br/>
 +
            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
 +
        </div>
 +
        <div class="f-img">
 +
          <div align="center"><img src="https://static.igem.org/mediawiki/2017/0/04/T-Nanjing-China-%E5%8D%97%E4%BA%AC%E5%A4%A7%E5%AD%A6.png" name="img1" width="80" height="110" id="img1" /></div>
 +
        </div>
 +
          <div class="f-img">
 +
            <div align="right"><img src=" https://static.igem.org/mediawiki/2017/d/d4/T-Nanjing-China-%E5%9B%BE%E7%89%873.png
 +
" name="img2" width="80" height="110" id="img2" /></div>
 +
          </div>
 +
          <div class="f-img">
 +
            <div align="center"><img src="https://static.igem.org/mediawiki/2017/3/37/T-Nanjing-China-s1.png" name="img3" width="80" height="80" id="img3" /></div>
 +
          </div>
 +
          <div class="f-img">
 +
            <div align="right"><img src="https://static.igem.org/mediawiki/2017/d/dc/T-Nanjing-China-%E5%9B%BE%E7%89%872.png" name="img4" width="80" height="80" id="img4"  /></div>
 +
        </div>
 +
      </div>
 +
      </div>
 +
      <div class="f-b"><img src="https://static.igem.org/mediawiki/2018/a/ad/T--Nanjing-China--footer-1.jpg" width="100%" /></div>
 +
  </div>
 +
</div>
 +
</body>
 
</html>
 
</html>

Revision as of 02:45, 27 April 2018

无标题文档

Liying Wang et. demonstrated a minimal nitrogen fixation gene cluster from Paenibacillus sp.WLY 78 which was compried of Pnif promoter and nine structural genes. Inspired by this study, we transferred this gene cluster to E.coli to create engineered E.coli cells which are capable of producing active nitrogenase. We achieved this by extracting the gene cluster from Paenibacillus sp.WLY78, connecting it to plasmid Psb1C3 and transformed it to E coli cells.In order to ensure the expression of this gene cluster in E coli,first we verified the transcriptional activity of Pnif promoter in E coli cells by conducting control experiments.In the test group,we replaced the native T5 promoter on pQE80L vector with Pnif,connected it to Dronpa fluorescent protein gene and transformed the new vectors to E.coli cells.In the control group, pQE80L vectors with T5 promoter and Dronpa gene were transformed to E coli cells. The comparable level of fluorescence intensity of the two groups indicated that Pnif promoter is transcriptional active in E coli cells.

play
stop

Simultaneously, in order to achieve in site synthesis of CdS nanocrystals on cell surfaces,we transformed the vector with opmA-PbrR gene to E.coli cells.This gene encodes opmA-PbrR protein complex,which can be fixed on cell surface by outer membrane protein (OMP) opmA.The function of PbrR protein is to adsorb Cd2+ in the environment and further form CdS nanocrystal on cell surface,a key component of this light-harvesting system. When this system is exposed by light, electrons from electron donor conduct transition while CdS nanocrystals on cell surfaces transfer high-energy electrons to Mo-Fe protein subunit of nitrogenase.Mo-Fe protein then utilizes the energy from these electrons rather than ATP to reduce dinitrogen into ammonia. With the method mentioned above, we successfully constructed a whole-cell light-driven nitrogen fixation system.

play
stop

In later period of our study, in order to elevate the electron transfer efficiency, we introduced Ag2S to realize Ag2S CdS cocatalysis. We respectively introduced ompA-spy tag Ag oligopeptide complex and ompA-spychcatcher-PbrR complex to E.coli cells. In that way, two types of E coli cells--with Ag2S or CdS nanocrystals adsorbed on surfaces specifically bind with each other through covalent bonds between spytag and spycatcher. Eventually, nitrogen fixation efficiency of our system showed a remarkable increase

play
stop

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china