Difference between revisions of "Team:UCSC/Experiments"
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<p class="p-title">Experiment 0</p> | <p class="p-title">Experiment 0</p> | ||
<p> Description in progress</p> | <p> Description in progress</p> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/2/2d/T--UCSC--Experiment_0_Overview.jpg" width=" | + | <img src="https://static.igem.org/mediawiki/2018/2/2d/T--UCSC--Experiment_0_Overview.jpg" width="50%" class="image-inpage"> |
<p class="image-inpage-caption" style="width:66%; font-size:85% !important"> Figure 1: Creation of pOPPY-UC19-yXXU containing URA3, pUC19 backbone, Lox sites and homologous arms using Gibson Assembly. Transformation of Y. lipolytica to create Y. lipolytica str. LipLox homologous recombination. </p> | <p class="image-inpage-caption" style="width:66%; font-size:85% !important"> Figure 1: Creation of pOPPY-UC19-yXXU containing URA3, pUC19 backbone, Lox sites and homologous arms using Gibson Assembly. Transformation of Y. lipolytica to create Y. lipolytica str. LipLox homologous recombination. </p> | ||
</div> | </div> | ||
Revision as of 18:34, 16 August 2018
Experiments
Overview
Description in progress
Experiment 0
Description in progress
Figure 1: Creation of pOPPY-UC19-yXXU containing URA3, pUC19 backbone, Lox sites and homologous arms using Gibson Assembly. Transformation of Y. lipolytica to create Y. lipolytica str. LipLox homologous recombination.
Experiment 1
Description in progress
Figure 2: Creation of pOPPY-19-yP via overlap extension PCR of genes involved in progesterone biosynthesis and gibson assembly of this gene cassette with homologous arms and linearized pUC19 backbone. Transformation of Y. lipolytica str. LipLox using homologous recombination to exchange gene cassette. Selection of Y. lipolytica str. PoPPY using 5FOA URA+.
Experiment 2
Description in progress
Experiment 3
Description in progress
Quantification
Description in progress