Difference between revisions of "Team:SKLMT-China/Notebook Protocol"

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            <h2 class="title">Article</h2>
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            <p class="content">content test</p>
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    <section class="container">
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            <li><a href="#"><i class="fa fa-home">Home</i></a></li>
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                        Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.
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                        Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.
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             <h2 class="title">Protocol</h2>
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             <h2 class="title" id="firstSection">Description</h2>
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             <p>test</p>
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             <p>Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.</p>
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            <p>Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.</p>
  
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            <h2 class="title">Another</h2>
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                <table>
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                    <thead>
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                        <tr>
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                            <th>Component</th>
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                            <th>Amount(ul)</th>
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                            <th>Final</th>
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                        </tr>
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                    </thead>
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                    <tbody>
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                        <tr>
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                            <td>Autoclaved ddH2O</td>
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                            <td>22</td>
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                            <td></td>
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                        </tr>
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                        <tr>
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                            <td>2×primeSTAR max premix</td>
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                            <td>25</td>
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                            <td>1×</td>
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                        </tr>
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                        <tr>
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                            <td>Template, 50ng ul<sup>-1</sup></td>
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                            <td>1</td>
 +
                            <td>50 ng</td>
 +
                        </tr>
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                        <tr>
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                            <td>Syn-promoter-3 Oligo(10uM)</td>
 +
                            <td>1</td>
 +
                            <td>30 pmol</td>
 +
                        </tr>
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                        <tr>
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                            <td>promoter-4 Oligo(10uM)</td>
 +
                            <td>1</td>
 +
                            <td>30 pmol</td>
 +
                        </tr>
 +
                    </tbody>
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                    <tfoot>
 +
                        <tr>
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                            <td>Total</td>
 +
                            <td>50</td>
 +
                        </tr>
 +
                    </tfoot>
 +
                </table>
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            </div>
  
 
         </div>
 
         </div>
 
     </div>
 
     </div>
  
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     <section class="wrapper style2 align-center" id="section-3">
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        <div class="inner">
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            <h2>Members</h2>
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            <p>Here are our <code>team</code> members.</p>
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        </div>
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 +
        <!-- Gallery -->
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        <div class="gallery big lightbox onscroll-fade-in">
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            <article>
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                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Zhao Wenxue" data-description="Zhao Wenxue is a 2nd year life science student. As a team leader she is responsible for the organizational aspects of the project, making sure that everything is going smoothly and according to the plan. She wants to be the heart and soul of a team and she always encourages every team member to try his or her best to accomplish this competition. In the lab she always transformed and she still has a dream that one day she will be able to improve the recombination efficiency dramatically. And she always prayed to agarose gel for her team to succeed in the Red/ET direct clone. Aside from iGEM, Zhao Wenxue spends her time promoting synthetic biology at Shandong University Faculty of Life Science." />
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                </a>
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                <div class="caption">
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                    <h3>Zhao Wenxue</h3>
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                    <p>Team Captain & Lab Manager</p>
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                    <ul class="buttons fixed">
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                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
 +
            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Zhao Xiaohan" data-description="Zhao Xiaohan is our &quot;Big Sister&quot;. Within the team she focused on the design of RecET direct cloningproject, as well as the experimental skill training for us. Xiaohan is active in thinking and did often come out with some constructive ideas, like the nicotine degradation one. It is so lucky to have her with us. She's enthusiastic and efficient, may be a little argumentative but not bossy. Our 'Big Sister' is a fervent fan of Mavel Cinematic Universe, so she prefers San Diego to Boston." />
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                </a>
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                <div class="caption">
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                    <h3>Zhao Xiaohan</h3>
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                    <p>Team Captain & Lab Manager</p>
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                    <ul class="buttons fixed">
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                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
 +
            <article>
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                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Tang Fei" data-description="This is Tang Fei, one of the student leader of SKLMT-CHINA. She is focus on our project and experimental progressing. she devotes a lot to set up the team, too. On the other hand, she is good at processing and plotting as well. We can be more confident because of her positive character. If coming to our lab, you will know how versatile she is. Not only can she play music but also paint well. When talking to her, you can get more details about our subject." />
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                </a>
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                <div class="caption">
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                    <h3>Tang Fei</h3>
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                    <p>Team Captain & Lab Manager</p>
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                    <ul class="buttons fixed">
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                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
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            <article>
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                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Tian Weixuan" data-description="Tian Weixuan majors in the college of Environmental Science and Engineering at school. (So you know, she isn’t as specific as other members in terms of biology.) However, she has rich experience in the organization process of the activities. She does some human practice works and pulls sponsorship for this team. Tian Weixuan really feels happy to join this outstanding team because she really admires students who do experiments dramatically and she would like to be highly skilled about experiments. Tian Weixuan is curious about new things and become crazy in front of close friends. She hopes to help her team with her enthusiasm and wisdom (hey hey!) BTW, she loves BTS!" />
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                </a>
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                <div class="caption">
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                    <h3>Tian Weixuan</h3>
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                    <p>Lab Manager & HP Manager</p>
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                    <ul class="buttons fixed">
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                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
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            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Dong Dongyang" data-description="Dong Dongyang, grade two, is a student in Life Science College of Shandong University. In Lab, she is extremely logical and motivated. In daily life, she is totally another girl, who is humorous and communicative. Her main work is about the bacteria machine &quot;S16&quot;. Besides, she is also in charge of our Interlab. Fond of playing volleyball, going hiking, and reading, Dongyang is really a nice girl with so much passion. We feels very happy to go together with her." />
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                </a>
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                <div class="caption">
 +
                    <h3>Dong Dongyang</h3>
 +
                    <p>Data Manager & Lab Manager</p>
 +
                    <ul class="buttons fixed">
 +
                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
 +
            </article>
 +
            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Sun Ao" data-description="Sun Ao is the tallest member of our wetlab. Within the team he worked on the gene recombination of nicotine degradation gene cluster. Also, He is a very &quot;stubborn&quot; guy. If you tell him that he can't make it, he would take several months just to refute your view. Remember one thing, never mention any star of electronic music in front of him, because he thinks only Daft punk can be called the superstar of electronic music. " />
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                </a>
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                <div class="caption">
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                    <h3>Sun Ao</h3>
 +
                    <p>Lab Manager</p>
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                    <ul class="buttons fixed">
 +
                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
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            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Liang Xueyuan" data-description="She is a girl who has a sunny disposition. She wants to be both a speaker of words and a doer of deeds in SKLMT-CHINA team. She focused on doing human practice and soliciting financial support. What's more, she also do some help in art designing work and experiment, and she often raised questions about our projects that made the experiment improve. Besides her excellent ability of preparing for iGEM, she is the one who loved dancing and music. All in all, Liang Xueyuan is a dynamic and important member of SKLMT-China." />
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                </a>
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                <div class="caption">
 +
                    <h3>Liang Xueyuan</h3>
 +
                    <p>Communication Manager & Finance Manager</p>
 +
                    <ul class="buttons fixed">
 +
                        <li><span class="actions small">Details</span></li>
 +
                    </ul>
 +
                </div>
 +
            </article>
 +
            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Li Hanzhen" data-description="Li Hanzhen, a girl who looks like a boy. As the team's competition affairs and art designer, she took charge of all the competition problems, contacted the cooperation team, and designed the team's logo, wiki and uniforms. More over, she also contributed to the design and improvement of the experiment. Liking all kinds of novelty, she always raises strange ideas, which advancing the project process. She is good at painting and design, and is very willing to use her skills to contribute to the building of the team." />
 +
                </a>
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                <div class="caption">
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                    <h3>Li Hanzhen</h3>
 +
                    <p>Designer & Competition Affairs</p>
 +
                    <ul class="buttons fixed">
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                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
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            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
 +
                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Shan Hongkun" data-description="Shan Hongkun is the joker in our team. In general, we call him a female name, penny, as he is treated as a delicate girl by other members. He is a part of human practice group and interlab group. Shan Hongkun is a regular night owl but usually boost his energy for works in the daytime. Working out in the gym is one of his recent hobby. He always has a preference in running and court games." />
 +
                </a>
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                <div class="caption">
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                    <h3>Shan Hongkun</h3>
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                    <p>Human Practice Manager</p>
 +
                    <ul class="buttons fixed">
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                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
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            <article>
 +
                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Chen Nuo" data-description="In Chinese, Chen Nuo means promise. In fact, our team member Chen Nuo is willing to solve problems on time as he promised. He provides technical support for our team, using his skills in both mathematics and computer science. He is quite easy to get along with. He deeply trusts all his friends, and he is happy to give a helping hand whenever his friends need. Besides, he is funny as well. He is proud of his &quot;Orchid Dance&quot; created by himself, and his dancing posture is vary elegant!" />
 +
                </a>
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                <div class="caption">
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                    <h3>Chen Nuo</h3>
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                    <p>Modeling Manager</p>
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                    <ul class="buttons fixed">
 +
                        <li><span class="actions small">Details</span></li>
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                    </ul>
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                </div>
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            </article>
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            <article>
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                <a href="https://static.igem.org/mediawiki/2018/a/a1/T--SKLMT-China--testFulls1.jpg" class="image">
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                    <img src="https://static.igem.org/mediawiki/2018/9/99/T--SKLMT-China--testThumbs1.jpg" data-name="Gao Dechen" data-description="Gao Dechen is a jack of all trades in computer science and many other fields. As the manager of our wiki, his sense of responsibility has made him more skilled in web designing and programming, which finally helped us finish our beautiful wiki. He also dabbles in graphic design for our wiki pages and poster, he learned these art skills after joining in our team. As a layman of biology, he still tried to get involved in our competition related affairs, giving some advice and making plans." />
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                    <h3>Gao Dechen</h3>
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                    <p>Wiki Manager</p>
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Revision as of 01:25, 14 October 2018

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Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.

Panel Title

Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.

Panel Title

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Description

Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.

Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.

Another

Component Amount(ul) Final
Autoclaved ddH2O 22
2×primeSTAR max premix 25 1×
Template, 50ng ul-1 1 50 ng
Syn-promoter-3 Oligo(10uM) 1 30 pmol
promoter-4 Oligo(10uM) 1 30 pmol
Total 50

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