Difference between revisions of "Team:WPI Worcester/Hardware"
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| − | <h1>Hardware: Low Cost Gene Gun</h1> | + | <h1>Hardware: Low-Cost Gene Gun</h1> |
<h2>Introduction</h2> | <h2>Introduction</h2> | ||
| − | <p></p> | + | <p> |
| + | A gene gun, or a biolistic particle delivery system, is a device to transform cells with exogenous DNA using DNA-coated microparticles. Biolistics is a versatile and important method for genetic modification, yet the price of a commercial gene gun could go up to $17,000, making it hard to afford for labs. | ||
| + | Inspired by the 2016 iGEM University of Cambridge team and online sources (Wernick, Hanson, Taylor & Arnie, 2016), out team built a low-cost gene gun costing only $416.35, with parts easily purchased online. Our gene-gun has improved safety features and simplified electronic components of previous designs. | ||
| + | This low-cost gene gun has been proved to be functional through test fires on lettuce leaves, which had cells successfully transformed with GFP. We look forward to testing the gene gun with AFPs to see if it could transform lettuce to produce AFP to protect itself from bacteria forming biofilms. | ||
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| − | <h2> | + | <h2>Key Components</h2> |
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Revision as of 00:11, 14 October 2018
Hardware: Low-Cost Gene Gun
Introduction
A gene gun, or a biolistic particle delivery system, is a device to transform cells with exogenous DNA using DNA-coated microparticles. Biolistics is a versatile and important method for genetic modification, yet the price of a commercial gene gun could go up to $17,000, making it hard to afford for labs. Inspired by the 2016 iGEM University of Cambridge team and online sources (Wernick, Hanson, Taylor & Arnie, 2016), out team built a low-cost gene gun costing only $416.35, with parts easily purchased online. Our gene-gun has improved safety features and simplified electronic components of previous designs. This low-cost gene gun has been proved to be functional through test fires on lettuce leaves, which had cells successfully transformed with GFP. We look forward to testing the gene gun with AFPs to see if it could transform lettuce to produce AFP to protect itself from bacteria forming biofilms.