Difference between revisions of "Team:SZU-China/Improve"

Line 1: Line 1:
 
{{SZU-China/header}}
 
{{SZU-China/header}}
 
<html>
 
<html>
 +
 +
<style>
 +
    h1,h2 {
 +
        color: #469789;
 +
    }
 +
   
 +
    .nav-pills .nav-link.active,
 +
    .nav-pills .show>.nav-link {
 +
       
 +
        color: #fff !important;
 +
        background-color: #469789;
 +
    }
 +
   
 +
    #myScrollspy>nav>a {
 +
        color: #469789;
 +
    }
 +
</style>
  
 
<body data-spy="scroll" data-target="#myScrollspy" data-offset="10">
 
<body data-spy="scroll" data-target="#myScrollspy" data-offset="10">
Line 11: Line 28:
 
<div class="row">
 
<div class="row">
 
<div class="col-9 offset-1 shadow ">
 
<div class="col-9 offset-1 shadow ">
 +
<div class="text-center"><h1>Improve</h1></div>
 +
<div class="row">
 +
<p>
 +
Last year,SZU-China submitted part C125-TupA(
 +
<a style="color: #469789;" href="http://parts.igem.org/Part:BBa_K2232003">BBa_K2232003</a>)
 +
, which is the coding sequence (CDS) of a cytoplasmic protein TupA (GenBank: BAB07375.1).This protein catalyzes the conversion of glucuronic acid and L-glutamic acid to polyglucuronic acid and poly-γ-L-glutamic acid.Besides this gene plays a key role in pH homeostasis and increases the alkali resistance of Bacteria.
 +
 +
</p>
 +
<p>
 +
But it’s a pity that there was no data at the protein level in 2017 SZU-China iGEM’s project.
  
 +
</p>
 +
<p>
 +
This year we decided to add a His-tag at the C-terminus of the protein,so that we can gain this cytoplasmic protein by Histidine-labeled affinity column chromatography.,and analysis at the protein level is easier.
  
 +
</p>
 +
<p>
 +
We performed Histidine-labeled affinity column chromatograpghy to isolate fusion protein,then verified by SDS-PAGE and Coomassie blue staining.(Fig.1)
  
 +
</p>
 +
</div>
 +
  
−
</div>
 
  
 +
 +
</div>
 +
<!--
 
<div class="col-2">
 
<div class="col-2">
  
Line 28: Line 66:
  
 
</div>
 
</div>
−
 
+
-->
 
</div>
 
</div>
  
Line 34: Line 72:
  
 
</body>
 
</body>
 +
  
  
  
 
</html>
 
</html>

Revision as of 11:37, 16 October 2018

Improve

Last year,SZU-China submitted part C125-TupA( BBa_K2232003) , which is the coding sequence (CDS) of a cytoplasmic protein TupA (GenBank: BAB07375.1).This protein catalyzes the conversion of glucuronic acid and L-glutamic acid to polyglucuronic acid and poly-γ-L-glutamic acid.Besides this gene plays a key role in pH homeostasis and increases the alkali resistance of Bacteria.

But it’s a pity that there was no data at the protein level in 2017 SZU-China iGEM’s project.

This year we decided to add a His-tag at the C-terminus of the protein,so that we can gain this cytoplasmic protein by Histidine-labeled affinity column chromatography.,and analysis at the protein level is easier.

We performed Histidine-labeled affinity column chromatograpghy to isolate fusion protein,then verified by SDS-PAGE and Coomassie blue staining.(Fig.1)