Difference between revisions of "Team:SZU-China/Simulation"

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<h1>Simulation</h1>
 
<h1>Simulation</h1>
 
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<h3>Design</h3>
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Because we have learned that the chemical method has a certain driving effect on killing, the driving
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Based on the actual size of the dormitory, we built a simulated room with the KT version and designed a walkway. The operation of the whole experiment is like this. Our experiments are divided into three groups. control group, the chemical group, and the biological group.
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efficiency in the home environment will cause great trouble to the neighbors. At present, the driving
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effect of biopharmaceutics is not clear. To compare the killing effect and driving effect of chemical  
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agents and our biopharmaceuticals in a simulation experiment, we chose the two best-selling and best-
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evaluated chemicals on the market for testing, namely <i>chalk and Bactericide</i>.
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<h4>Control group</h4>
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The control group did not add any pharmaceutical agents.
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<h4>Chemical group</h4>
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<div class="text-center">The chemical group was divided into two groups, namely the chalk group and the Bayer acaricide bait group</div>
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<h4>Biological group</h4>
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The biological group was placed with the second version of the drug-coated box.
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<div class="indent">
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Based on the actual size of the dormitory, we built a simulated room with the KT version and designed a walkway.
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<h3>Experiment</h3>
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The operation of the whole experiment is like this. Our experiments are divided into three groups, which are
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the control group, the chemical group, and the biological group. The control group did not add any
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pharmaceutical agents. The chemical group was divided into two groups, namely the chalk group and the Bayer
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acaricide bait group, and the biological group was placed with the second version of the drug-coated box.
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We will place 30 sputa in one of the rooms (Room A). The experimental period of each group is 3 days, and
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the parallel experimental group of each group is 3 groups. The main recorded data are sputum mortality,
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mobility (in the aisle and B). The cockroaches of the room are considered to have been migrated.) The purpose
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of setting up a blank group is to explore what the mobility and normal mortality of cockroaches are without
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the influence of any medicinal agents, and can provide a reference for our analysis of chemical and biological
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data.
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<h1>Statistical Analysis Results</h1>
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Our data were subjected to statistical analysis to determine whether there are significant differences using one-way ANOVA. Levene’s test, gave us results that each group is homogeneity at P-value of 0.05, indicating we can assume they have equal variances. T-test for pooled data gave us results that: migration rate infected by M.anisopliae emulsifiable powder was significantly lower than that by cockroach killing chalk or BAYER-Premise (P<0.05). While, there was no significant difference in migration rate between cockroach killing chalk and BAYER-Premise (P>0.05). Similarly, gnawing rate in M.anisopliae group was significantly higher than that in cockroach killing chalk or BAYER-Premise group (P<0.05), while between the latter two groups, there was no significant difference(P>0.05). As for mortality within three days, there was no significant difference between each two groups (P>0.05).
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We will place 30 sputa in one of the rooms (Room A). The experimental period of each group is 3 days, and the parallel experimental group of each group is 3 groups. The main recorded data are sputum mortality, mobility (in the aisle and B).
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</p>
 
</p>
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<p>
 
<p>
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The results above show that M.anisopliae emulsifiable powder has higher efficiency than other traditional cockroach-killing methods. It has significantly lower migration rate, higher gnawing rate, and has similar mortality after using
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The cockroaches of the room are considered to have been migrated.) The purpose of setting up a blank group is to explore what the mobility and normal mortality of cockroaches are without the influence of any medicinal agents, and can provide a reference for our analysis of chemical and biological data.
 
</p>
 
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<img class="card-img-top" src="https://static.igem.org/mediawiki/2018/4/40/T--SZU-China--HP_sim_4.jpeg" alt="Card image cap">
  
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<p class="text-center">our experiments</p>
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          <h4>Statistical Analysis Results</h4>
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As is shown on the chart, we can analyze that Cockroach killing chalk and BAYER-Premise have a big impact on Migration Rate, they both cause it higher than the Blank Group, while the M.anisopliae emulsifiable powder’s is lower than those three group, this shows that the M.anisopliae emulsifiable powder is more attractive to cockroaches and has less impact on neighbors. From the perspective of mortality, these three groups do not have much difference. But still, the M.anisopliae emulsifiable powder Group has the highest mortality. From the point of view of the rate of Gnawing, in the natural situation, there is a similar behavior that cockroaches gnaw each other, but the M.anisopliae emulsifiable powder can enhance this situation, that means this can increase the probability of fungus spreading between cockroaches.
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<p>From the perspective of mortality, these three groups do not have much difference. But still, the M.anisopliae emulsifiable powder Group has the highest mortality.
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</p>
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<p>
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From the point of view of the rate of Gnawing, in the natural situation, there is a similar behavior that cockroaches gnaw each other, but the M.anisopliae emulsifiable powder can enhance this situation, that means this can increase the probability of fungus spreading between cockroaches.
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</p>
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Based on the above results, our <i>M.anisopliae</i> emulsifiable powder <b>has higher efficiency than other traditional cockroach killing methods. It has a low mobility, a higher gnawing rate,
 +
and mortality similar to other drugs. </b> Detailed and more scientific analysis results can be found in our
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<a href="https://2018.igem.org/Team:SZU-China/Model">modeling section</a>.
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Revision as of 11:19, 14 October 2018

Simulation

Design

Based on the actual size of the dormitory, we built a simulated room with the KT version and designed a walkway. The operation of the whole experiment is like this. Our experiments are divided into three groups. control group, the chemical group, and the biological group.

Control group

The control group did not add any pharmaceutical agents.

Chemical group

The chemical group was divided into two groups, namely the chalk group and the Bayer acaricide bait group

Biological group

The biological group was placed with the second version of the drug-coated box.

Experiment

We will place 30 sputa in one of the rooms (Room A). The experimental period of each group is 3 days, and the parallel experimental group of each group is 3 groups. The main recorded data are sputum mortality, mobility (in the aisle and B).

The cockroaches of the room are considered to have been migrated.) The purpose of setting up a blank group is to explore what the mobility and normal mortality of cockroaches are without the influence of any medicinal agents, and can provide a reference for our analysis of chemical and biological data.

Card image cap
Card image cap

our experiments

As is shown on the chart, we can analyze that Cockroach killing chalk and BAYER-Premise have a big impact on Migration Rate, they both cause it higher than the Blank Group, while the M.anisopliae emulsifiable powder’s is lower than those three group, this shows that the M.anisopliae emulsifiable powder is more attractive to cockroaches and has less impact on neighbors. From the perspective of mortality, these three groups do not have much difference. But still, the M.anisopliae emulsifiable powder Group has the highest mortality. From the point of view of the rate of Gnawing, in the natural situation, there is a similar behavior that cockroaches gnaw each other, but the M.anisopliae emulsifiable powder can enhance this situation, that means this can increase the probability of fungus spreading between cockroaches.

From the perspective of mortality, these three groups do not have much difference. But still, the M.anisopliae emulsifiable powder Group has the highest mortality.

From the point of view of the rate of Gnawing, in the natural situation, there is a similar behavior that cockroaches gnaw each other, but the M.anisopliae emulsifiable powder can enhance this situation, that means this can increase the probability of fungus spreading between cockroaches.

Based on the above results, our M.anisopliae emulsifiable powder has higher efficiency than other traditional cockroach killing methods. It has a low mobility, a higher gnawing rate, and mortality similar to other drugs. Detailed and more scientific analysis results can be found in our modeling section.