Difference between revisions of "Team:SKLMT-China/Composite Part"
| Line 38: | Line 38: | ||
<p>Our submitted parts include a promoter library of inner promoters with various strength built in <latin>Pseudomonas fluorescence</latin>-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhancing nicotine degradation compared to original nicA2 gene. </p> | <p>Our submitted parts include a promoter library of inner promoters with various strength built in <latin>Pseudomonas fluorescence</latin>-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhancing nicotine degradation compared to original nicA2 gene. </p> | ||
</div> | </div> | ||
| + | <div class="paragraph shadow" id="s1"> | ||
| + | <h2 class="title">PdnaA-NicA2 </h2> | ||
| + | |||
| + | <p>We used SDS-PAGE, to demonstrate the expression of NicA2 (52.5 kDa). In the picture, we can see the clear expression of NicA2 in <i>P.</i>pf-5 containing plasmid with our promoter. It can be qualitatively known that our promoters can initiate transcription normally, and the nicotine-degrading gene cluster has also been successfully heterologously expressed.</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/e/ea/T--SKLMT-China--demonstrationfig2.png | ||
| + | " alt="Demonstration of NicA2 expression via SDS-PAGE" /> | ||
| + | </div> | ||
<div class="paragraph shadow" id="s2"> | <div class="paragraph shadow" id="s2"> | ||
Revision as of 11:29, 17 October 2018
Overview
Our submitted parts include a promoter library of inner promoters with various strength built in
PdnaA-NicA2
We used SDS-PAGE, to demonstrate the expression of NicA2 (52.5 kDa). In the picture, we can see the clear expression of NicA2 in P.pf-5 containing plasmid with our promoter. It can be qualitatively known that our promoters can initiate transcription normally, and the nicotine-degrading gene cluster has also been successfully heterologously expressed.
Composite Parts
| Biobrick | Type | Description | length |
| BBa_K2569031 | Composite | PdnaA -NicA2 | 1525 |
| BBa_K2569032 | Composite | PdnaA-mRFP | 782 |