Difference between revisions of "Team:SKLMT-China/Basic Part"
| Line 7: | Line 7: | ||
</div> | </div> | ||
<div class="container"> | <div class="container"> | ||
| + | <h2 class="title">Overview </h2> | ||
| + | <p>Our submitted parts include a promoter library of inner promoters with various strength built in latin<>Pseudomonas fluorescence</latin>-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene. </p> | ||
| + | <h2 class="title"> Promoter Library</2> | ||
| + | <p>We are proud to submit a promoter library of inner promoters with various strength built in <i>Pseudomonas fluorescence</i>-pf5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in <i>Pseudomonas fluorescence</i>-pf5. Additionally, this promoter library will support future iGEM teams some help in exploration on <i>Pseudomonas fluorescence</i>-pf5 cells with high capacity in degrading. </p> | ||
| + | <p>We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/9/9b/T--SKLMT-China--promoter_y.png" alt="Measurement of promoter strength via measuring firefly fluorescein" /> | ||
| + | |||
| + | <p>According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively. </p> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/e/e3/T--SKLMT-China--promoter_outputy.png | ||
| + | " alt="Strength level of different promoters" /> | ||
| + | |||
| + | <h2 class="title"> All Basic Parts</2> | ||
| + | |||
| + | <table><thead><tr><td>Biobrick</td><td>Type</td><td>Description</td><td>length</td></tr><tr><td> </td><td> </td><td> </td><td> </td></tr></thead><tbody><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569001">BBa_K2569001</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569002">BBa_K2569002</a></td><td>Regulatory</td><td>ParaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569003">BBa_K2569003</a></td><td>Regulatory</td><td>PfabD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569005">BBa_K2569005</a></td><td>Regulatory</td><td>PccmD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569006">BBa_K2569006</a></td><td>Regulatory</td><td>PfliA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569011">BBa_K2569011</a></td><td>Regulatory</td><td>PpltR, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569012">BBa_K2569012</a></td><td>Regulatory</td><td>PproC, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569013">BBa_K2569013</a></td><td>Regulatory</td><td>PrecA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569014">BBa_K2569014</a></td><td>Regulatory</td><td>PrpoD, a constitutive promoter in pseudomonas fluorescence</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569015">BBa_K2569015</a></td><td>Regulatory</td><td>PrpoS, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569016">BBa_K2569016</a></td><td>Regulatory</td><td>PdnaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569017">BBa_K2569017</a></td><td>Regulatory</td><td>Ppol, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569018">BBa_K2569018</a></td><td>Regulatory</td><td>P16s, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569019">BBa_K2569019</a></td><td>Regulatory</td><td>Psig, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569020">BBa_K2569020</a></td><td>Regulatory</td><td>Patf, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569021">BBa_K2569021</a></td><td>Regulatory</td><td>Pfme, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569022">BBa_K2569022</a></td><td>Regulatory</td><td>Pmem, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569023">BBa_K2569023</a></td><td>Regulatory</td><td>Pfat, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569024">BBa_K2569024</a></td><td>Regulatory</td><td>Pami , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569025">BBa_K2569025</a></td><td>Regulatory</td><td>Prib, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569026">BBa_K2569026</a></td><td>Regulatory</td><td>Pabc , constitutive promoter in pseudomonas fluorescence pf-4</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569027">BBa_K2569027</a></td><td>Regulatory</td><td>Pedo , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569028">BBa_K2569028</a></td><td>Regulatory</td><td>Ptox , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569029">BBa_K2569029</a></td><td>Regulatory</td><td>PampC , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569030">BBa_K2569030</a></td><td>Regulatory</td><td>Plip , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569033">BBa_K2569033</a></td><td>Regulatory</td><td>PampR,promoter</td><td>92</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569034">BBa_K2569034</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569036">BBa_K2569036</a></td><td>Coding</td><td>Nicotine oxidase NicA2</td><td>1449</td></tr><tr><td> </td></tr></tbody></table><br /><span style="font-family:Arial;"><br /></span><p><span style="font-size: 10.5pt;"><span style="font-family:Arial;"> </span></span></p> | ||
<div class="row"> | <div class="row"> | ||
Revision as of 13:45, 17 October 2018
Our submitted parts include a promoter library of inner promoters with various strength built in latin<>Pseudomonas fluorescence-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene. We are proud to submit a promoter library of inner promoters with various strength built in Pseudomonas fluorescence-pf5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in Pseudomonas fluorescence-pf5. Additionally, this promoter library will support future iGEM teams some help in exploration on Pseudomonas fluorescence-pf5 cells with high capacity in degrading. We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data. According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively. Overview
Promoter Library
All Basic Parts
Biobrick Type Description length BBa_K2569001 Regulatory Pdiv, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569002 Regulatory ParaA, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569003 Regulatory PfabD, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569005 Regulatory PccmD, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569006 Regulatory PfliA, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569011 Regulatory PpltR, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569012 Regulatory PproC, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569013 Regulatory PrecA, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569014 Regulatory PrpoD, a constitutive promoter in pseudomonas fluorescence 70 BBa_K2569015 Regulatory PrpoS, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569016 Regulatory PdnaA, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569017 Regulatory Ppol, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569018 Regulatory P16s, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569019 Regulatory Psig, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569020 Regulatory Patf, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569021 Regulatory Pfme, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569022 Regulatory Pmem, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569023 Regulatory Pfat, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569024 Regulatory Pami , constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569025 Regulatory Prib, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569026 Regulatory Pabc , constitutive promoter in pseudomonas fluorescence pf-4 70 BBa_K2569027 Regulatory Pedo , constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569028 Regulatory Ptox , constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569029 Regulatory PampC , constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569030 Regulatory Plip , constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569033 Regulatory PampR,promoter 92 BBa_K2569034 Regulatory Pdiv, constitutive promoter in pseudomonas fluorescence pf-5 70 BBa_K2569036 Coding Nicotine oxidase NicA2 1449