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Revision as of 14:14, 17 October 2018

Template loop detected: Template:Grenoble-Alpes

ATTRIBUTIONS

Through this page, we want to explain the role that each team member has had within the project. We want also to make known, and thank, the other actors of the project who participated in its smooth running.
But before that we will present rapidly our iGEM Grenoble history!

iGEM GRENOBLE-ALPES STORY

1) How did we know iGEM ?

We have never had lessons on iGEM but each member of the team has heard about the competition at Grenoble Alpes University (UGA) or Grenoble INP school, by the members of the previous team and the teachers who took them framed.
Then, the team met last November via Pierre Cavailles (the main advisor and a biotechnology teacher at the UGA), and that's how the story started.

2) Project Chronology

You can find, in the diagram below, the chronology of the project which summarizes the key points of our work.



ATTRIBUTIONS

This part describes the work done, and the person who performed it.
The legend below could help you to know if it is a team member, an advisor, an instructor or an outsider thanks to the color code :

Team members
Advisors
Instructors
Outsiders

Engineering

WHAT THE TEAM DID

DNA extraction module & Phages-bacteria Modeling
Thomas Goyet

Temperature control system & Transformation Module
Xandre Gallard

Fluorescence Sensor module
Charles Jabour

Pipetting Module
Jean Le Pennec

Overall 3D Model of the system
Mainly Lucas Sancéré with the help of Jean Le Pennec & Thomas Goyet

Overall code for the control of the system
Jean Le Pennec & Thomas Goyet

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

Lab and premise support

Ugo Hevin, Franz Bruckert & Marianne Weidenhaupt of the CIME Nanotech Laboratory (Minatec, Grenoble) Nicolas Ruty & Julien Traveaux of the Phelma-INP school

Technical support

Engineers of BioMérieux : Hervé Rostaing (general engineering support), Blaze Jérome (mechanics and machining of complex parts of the system), Frédéric Pinston (fluorescence detection) & Alain Laurent (magnetic beads).

Biology

WHAT THE TEAM DID

Experiments on bacteriophages & their capacity to lyse bacteria
Cécile Razimbaud & Yasmine Taveau

DNA extraction with magnetic beads
Cécile Razimbaud

Construction of the DNA probe :
- detecting an antibiotic resistance marker of Pseudomonas a. PA01
Elise Aubert
- detecting a sequence of an housekeeping gene of Pseudomonas a. PA01
Fanny Garon

Conservation of biological products & Freeze-drying
Perrine Rochas

BBa_J04450 characterisation
Perrine Rochas with the help of Xandre Gallard

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

Lab support

Sophie Boretti, Martin Ghis and “practical work” service members in general (first floor of Jean Roger Laboratory (University of Grenoble-Alpes)).
The fifth floor members of Jean Roger Laboratory including Pierre Cavailles.
Hélène Coradin, Béatrice Schaack (for her freeze-drying help), and the other members of the eighth floor of Jean Roger Laboratory.


Biology support

Pierre Cavailles & Clément Caffaratti who provided a lot of advice about the Biology and an outstanding support throughout the project.


Bacteriophages experiments support - General support & donation of T5 bacteriophages and their host E. coli F

Cécile Breyton of the Grenoble IBS (Institute of Structural Biology)


Donation of Pseudomonas a. PA01

Ina Attrée of the Grenoble CEA (Office of the Atomic Energy and Alternative Energies)


Donation and sending of PAK-P3 bacteriophages and their host Pseudomonas a. PAK

Laurent Debarbieux of the Pasteur Institute of Paris


Donation of Staphylococcus aureus strains and a specific bacteriophage Sb-1

Grégory Resch & Jonathan Save of the University of Lausanne (Swiss)


Feedback and advices about detection experiments on bacteriophages

Pierre Marcoux of the Grenoble CEA (Office of the Atomic Energy and Alternative Energies)

FURTHER INFORMATIONS AND ACKNOWLEDGEMENTS

Pseudomonas aeruginosa PAO1 and their specific phages HER18 the team needed to find a class 2 laboratory which accepts to welcome us. Javier Yugueros Marcos, the main BioMérieux contact, have immediately responded to this call for help. With the assistance of Patrice Gracieux, he organized the coming of Cécile Razimbaud in BioMérieux lab (about security and others). Stéphanie Borland has accompanied Cécile during all the experiments, mostly for the safety rules.

Human Practices

WHAT THE TEAM DID

Human Practices Leader
Lucas Sancéré

Interviews with doctors in Grenoble
Lucas Sancéré & Cécile Razimbaud

Presentation and interview with future doctors and pharmacists
Fanny Garon, Cécile Razimbaud & Thomas Goyet

Interview with a patient who received a phage therapy
Elise Aubert & Fanny Garon

Interview with a member of Oriade, a medical biology laboratory
Xandre Gallard, Jean Le Pennec & Lucas Sancéré

Science popularizing videos overview & Survey design
Lucas Sancéré

FMECA study
Xandre Gallard

Scientific popularization video
-Script
Elise Aubert, Thomas Goyet & Charles Jabour
-Recording Fanny Garon, Jean Le Pennec & Lucas Sancéré
-Animations and video assembly
Perrine Rochas & Xandre Gallard

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

Main ethical reflection, survey design & other HP support

Loïc Lasek


Support for the FMECA Study

Bruno Commere

Interviewees

Pr Max Maurin (chief in the bacteriology service in Grenoble Hospital)
Pr Olivier Epaulard (PUPH in infectious diseases and tropical medicine service in Grenoble Hospital)
Mr. X (who received a phage therapy)
Students in Medicine and Pharmacy in Université Grenoble Alpes
Pierre-Alain Falconnet from Oriade
Terence Ericson (help with the scientific popularization video)

Graphic design, Webdesign & Animation

WHAT THE TEAM DID

Graphic visuals : videos, diagrams & Wiki design and coding
Xandre Gallard & Perrine Rochas with the help of Jean Le Pennec & Charles Jabour

Poster design
Elise Aubert with the help of other teams members

Logo design & other visuals
Sarah Ignace

Finale presentation for the Giant Jamboree
Xandre Gallard

3D model texturing and animation
Perrine Rochas

Presentation teaser
Elise Aubert & Perrine Rochas

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

Main wiki code

Maxime Chevaux


System videos

INProd


Team and individual photos

Fondation UGA's photograph
Loïc Lasek
Clément Caffarati


Grenoble view timelapse for the Wiki main page

Martin Bozon



NB: All the photos used within the Wiki are royalty-free and free to modify for non-commercial purposes

Events & Communication

WHAT THE TEAM DID

Forum Biotechno
Xandre Gallard, Charles Jabour, Yasmine Taveau & Perrine Rochas with the help of Romy Rouzeau, an ex iGEM Grenoble team member (2017)

UGA C’est Party
Charles Jabour, Perrine Rochas, Jean Le Pennec, Cécile Razimbaud, Lucas Sancéré, Xandre Gallard, Yasmine Taveau & Vincent Radziejewski

“Parvis des Sciences” - GIANT campus, Minatec
Thomas Goyet, Charles Jabour, Lucas Sancéré, Yasmine Taveau & Vicent Radziejewski

Midi Minatec
Fanny Garon, Cécile Razimbaud, Thomas Goyet & Charles Jabour have presented the project.
Xandre Gallard, Jean Le Pennec, Lucas Sancéré, Loïc Lasek & Vincent Radziejewski were present too on the stage.
Pierre Cavailles was in the public.

MeetUps :
- in Paris
Elise Aubert & Thomas Goyet
- in Marseille
Fanny Garon, Elise Aubert & Lucas Sancéré with Clément Caffaratti
- in Barcelona
Lucas Sancéré

INP associations forum
Thomas Goyet, Jean Le Pennec & Vincent Radziejewski

PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:

General communication support and advice

Vincent Radziejewski


Sponsor, organisation of the Crowdfunding and communication support

Mélanie Santailler & Nathalie Martino of the Fondation Université Grenoble-Alpes


Additional thanks

Project support and advice

- Pierre Cavailles, a remarkable teacher who supervised us throughout the project. We thank him for his general guideline.
- Clément Caffaratti , who, thanks to his iGEM 2017 experience and his passion for the biotechnologies, provided a lot of advices.
- Loïc Lasek , our Human Practices advisor who helped us thanks to his philosophical reflexion. Loïc provided us a beneficial support.
- Vincent Radziejewski, our Communication advisor who helped us to organize all the events and to establish all the other communication medium (network, goodies, …)
- BioMérieux, especially Javier Yugueros Marcos who supported us considerably throughout the project, as well as Blaze Jérôme, Hervé Rostaing, Frédéric Pinston, Alain Laurent and Christine Beni-Aiche. Their help allowed us to overcome a lot of problems. We are grateful to them for good cooperation.
- Audrey Le Gouellec who supervised the project.


Presentation coaching

Pierre Cavailles, Audrey Le Gouellec & Clément Caffaratti. Thomas Soranzo who inspired us for the theme of the presentation.


Teams that inspired the project

iGEM Grenoble (France) 2017 & iGEM Cork (Ireland) 2015

Supervisors of the student internship

Cordelia Bisanz, Pierre Cavailles, Audrey Le Gouellec, Franz Bruckert, Marianne Weidenhaupt, Nicolas Ruty and Fanny Poinsotte.


Meeting with Pherecydes Pharma

Javier Yugueros Marcos (BioMérieux) who put us in touch with Pherecydes Pharma, a company that develops phages. He helped Thomas Goyet and Yasmine Taveau to prepare an interview a video call interview with Guy-Charles Fanneau de La Horie President of Pherecydes Pharma, Laure Saujet Director Research and Development, Jérome Gabard General Manager. The goal of this interview was to propose a partnership and ask them a technical and material support.


iGEM collaboration

Munich iGEM team
Nottingham iGEM team


Contact for the iGEM competition

Abigail Sison who always answered really fast when we had questions !


Sponsors

Fondation UGA, Fondation Grenoble INP, UGA, Grenoble INP, BioMérieux , IDEX, FSDIE, TIMC, LDLC Grenoble, NEB, Grenoble-Alpes Métropole, Crous Grenoble Alpes, CNRS, IDT, Embassy of France in the US.


Finally we want to thank all people who have daily supported us and followed us!