In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:
Line 257: | Line 257: | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
<br> | <br> | ||
− | <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4> | + | <h4><a href="http://parts.igem.org/Part:BBa_K2760021:Design">BBa_K2760021 - SP2-Nsp2 Characterization construct</a></h4><br> |
− | + | <center> | |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image"> | + | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a2/T--TecMonterrey_GDL--Parts--7.png" alt="service-image"> |
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
Line 277: | Line 278: | ||
<br><br> | <br><br> | ||
<h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 - Negative control for signal peptide characterization</a></h4> | <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760020">BBa_K2760020 - Negative control for signal peptide characterization</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/04/T--TecMonterrey_GDL--Parts--8.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
Line 287: | Line 290: | ||
<div class="col-md-6"> | <div class="col-md-6"> | ||
<h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4> | <h4><a href="http://parts.igem.org/partsdb/edit_seq.cgi?part=BBa_K2760022">BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/08/T--TecMonterrey_GDL--Parts--9.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_I712074">BBa_I712074</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_Z0262">BBa_Z0262</a>, <a href="http://parts.igem.org/Part:BBa_K2760001">BBa_K2760001</a>, <a href="http://parts.igem.org/Part:BBa_K2760024">BBa_K2760024</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760012">BBa_K2760012</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_B0015">BBa_B0015</a></p> | ||
Line 311: | Line 316: | ||
<br><br><br><br><br><br> | <br><br><br><br><br><br> | ||
<h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4> | <h4><a href="http://parts.igem.org/Part:BBa_K2760030">Sp1-Nsp4- Characterization construct Part: BBa_K2760030</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/a/a1/T--TecMonterrey_GDL--Parts--10.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760000">BBa_K2760000</a>, | ||
Line 323: | Line 330: | ||
<br><br><br><br><br><br> | <br><br><br><br><br><br> | ||
<h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4> | <h4><a href="http://parts.igem.org/Part:BBa_K2760031:Design#References">Sp2-Nsp2- Characterization Construct Part: BBa_K2760031</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Parts--11.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, <a href="http://parts.igem.org/Part:BBa_K2760004">BBa_K2760004</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760029">BBa_K2760029</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760007">BBa_K2760007</a></p> | ||
Line 342: | Line 351: | ||
<br><br><br> | <br><br><br> | ||
<h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4> | <h4><a href="http://parts.igem.org/Part:BBa_K2760032">Sp negative control construct: Part BBa_2760032</a></h4> | ||
− | < | + | <br> |
− | <img class="img-responsive" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image"> | + | <center> |
+ | <img class="img-responsive" width="60%" src="https://static.igem.org/mediawiki/2018/3/37/T--TecMonterrey_GDL--Parts--12.png" alt="service-image"> | ||
+ | </center> | ||
<p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, | <p style="text-align:center">Sub parts: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760009">BBa_K2760009</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2760005">BBa_K2760005</a>, <a href="http://parts.igem.org/Part:BBa_K2760006">BBa_K2760006</a>, |
Revision as of 22:09, 17 October 2018
Parts
For E.coli
Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill
Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760003, BBa_K2760012, BBa_K2760029
For L. rhamnosus GG
Plan B (L. rhamnosus): Part: BBa_K276004
Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760006, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000, BBa_K2760003, BBa_K2760012, BBa_K2760029, BBa_K2760008
Signaling peptides
For the excretion of recombinant proteins
For E.coli
BBa_K2760021 - SP2-Nsp2 Characterization construct
Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760004, BBa_K2760024, BBa_K2760012, BBa_B0015
BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)
Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760001, BBa_K2760024, BBa_K2760012, BBa_B0015
For L.rhamnosus
Sp2-Nsp2- Characterization Construct Part: BBa_K2760031
Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760004, BBa_K2760029, BBa_K2760007