Difference between revisions of "Team:TecMonterrey GDL/Protocols"

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<nav class="navbar navbar-default">
 
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       <img src="https://static.igem.org/mediawiki/2018/e/e3/T--TecMonterrey_GDL--Home--logo.png" width="80">
 
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Dry lab<span class="caret"></span></a>
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Dry labs<span class="caret"></span></a>
 
                   <ul class="dropdown-menu">
 
                   <ul class="dropdown-menu">
 
                     <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Parts">Parts</a></li>
 
                     <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Parts">Parts</a></li>
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                   <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results<span class="caret"></span></a>
 
                   <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Results<span class="caret"></span></a>
 
                   <ul class="dropdown-menu">
 
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                     <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE">Genetic engineering</a></li>
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                     <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/GeneticE">Generic engineering</a></li>
 
                     <li role="separator" class="divider"></li>
 
                     <li role="separator" class="divider"></li>
 
                     <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Demonstrate">Biomimetric Tri-cultures</a></li>
 
                     <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Demonstrate">Biomimetric Tri-cultures</a></li>
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               <embed src="images/notebook/protocols/ecoli/1._LB_medium_preparation.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/2/26/T--TecMonterrey_GDL--Protocols--1._LB_medium_preparation.pdf" download="MediumPreparation">View Preparation of LB Medium</a>
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            </center>
 
           </div>
 
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           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/1.1_LB_medium_and_LB_agar-plates_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Protocols--1.1_LB_medium_and_LB_agar-plates_protocol.pdf" download="MediumPreparation">View LB medium and LB agar-plates Protocol</a>
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            </center>
 
           </div>
 
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           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/2._Electro_competent_cells_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/c/c0/T--TecMonterrey_GDL--Protocols--2._Electro_competent_cells_protocol.pdf" download="MediumPreparation">View Competent cells</a>
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            </center>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
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               <embed src="images/notebook/protocols/ecoli/3._Transformation_by_electroporation.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Protocols--3._Transformation_by_electroporation.pdf" download="MediumPreparation">View Transformation by electroporation</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/3.1_Competent_cells_BL21.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._3.1_Competent_cells_BL21.pdf" download="MediumPreparation">View Calcio competent cells</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/3.3_Ligation_by_iGEM.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/a/a3/T--TecMonterrey_GDL--Protocols--3.3_Ligation_by_iGEM.pdf" download="MediumPreparation">View Ligation and digestion of G-Blocks</a>
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            </center>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
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               <embed src="images/notebook/protocols/ecoli/3.4_Transformation_heat_shock_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/1/13/T--TecMonterrey_GDL--Protocols--3.4_Transformation_heat_shock_protocol.pdf" download="MediumPreparation">View Transformation Heat Shock</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/4._Miniprep_kit.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/c/c4/T--TecMonterrey_GDL--Protocols--4._Miniprep_kit.pdf" download="MediumPreparation">View MINIPREP KIT</a>
 +
            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/5._PCR.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/9/99/T--TecMonterrey_GDL--Protocols--5._PCR.pdf" download="MediumPreparation">View PCR</a>
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            </center>
 
           </div>
 
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               <embed src="images/notebook/protocols/ecoli/5._Horizontal_electroforesis_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/0/0b/T--TecMonterrey_GDL--Protocols--5._Horizontal_electroforesis_protocol.pdf" download="MediumPreparation">View Agarose Gel Electrophoresis</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
           <div class="col-md-4">
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               <embed src="images/notebook/protocols/ecoli/5._Optimizing_restriction_EcoR_I_y_Spe_I.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--5._Optimizing_restriction_EcoR_I_y_Spe_I.pdf" download="MediumPreparation">View Optimizing Restriction Endonuclease Reactions</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/6._Lysis_and_protein_purification.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/4/4d/T--TecMonterrey_GDL--Protocols--6._Lysis_and_protein_purification.pdf" download="MediumPreparation">View Protein extraction and purification based on Thermo Fisher's Protocol</a>
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            </center>
 
           </div>
 
           </div>
 
         </div>
 
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           </div>
 
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               <embed src="images/notebook/protocols/ecoli/7._SDS-PAGE_+_coomassie_staining_protocols.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/6/68/T--TecMonterrey_GDL--Protocols--7._SDS-PAGE_%2B_coomassie_staining_protocols.pdf" download="MediumPreparation">View SDS-PAGE</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/ecoli/8._Western_blot.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/0/0e/T--TecMonterrey_GDL--Protocols--8._Western_blot.pdf" download="MediumPreparation">View Western Blot based on Invitrogen's protocol</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-2">
 
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               <embed src="images/notebook/protocols/peptides/1._Electro_competent_cells_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._Electro_competent_cells_protocol.pdf" download="MediumPreparation">View Competent cells</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/peptides/2._Transformation_by_electroporation.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/d/d8/T--TecMonterrey_GDL--Protocols--2._Transformation_by_electroporation.pdf" download="MediumPreparation">View Transformation by electroporation</a>
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            </center>
 
           </div>
 
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           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/peptides/3.1_Competent_cells_BL21.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/1/14/T--TecMonterrey_GDL--Protocols--3.1_Competent_cells_BL21.pdf" download="MediumPreparation">View Calcio competent cells</a>
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            </center>
 
           </div>
 
           </div>
 
         </div>
 
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               <embed src="images/notebook/protocols/peptides/Digestion_and_ligation.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Protocols--Digestion_and_ligation.pdf" download="MediumPreparation">View Procedure of digestion of the constructo</a>
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            </center>
 
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           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/peptides/High_fidelity_restriction_enzymes_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/7/75/T--TecMonterrey_GDL--Protocols--High_fidelity_restriction_enzymes_protocol.pdf" download="MediumPreparation">View High Fidelity restriction enzyme Protocol</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/peptides/Miniprep.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/1/1d/T--TecMonterrey_GDL--Protocols--Miniprep.pdf" download="MediumPreparation">View MINIPREP KIT</a>
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            </center>
 
           </div>
 
           </div>
 
         </div>
 
         </div>
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           </div>
 
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               <embed src="images/notebook/protocols/peptides/PCR.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/3/34/T--TecMonterrey_GDL--Protocols-PCR.pdf" download="MediumPreparation">View PCR</a>
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            </center>
 
           </div>
 
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           <div class="col-md-4">
 
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               <embed src="images/notebook/protocols/peptides/Protein_expression_with_T7_strains.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/6/65/T--TecMonterrey_GDL--Protocols--Protein_expression_with_T7_strains.pdf" download="MediumPreparation">View Protein Expression with T7 Strains</a>
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            </center>
 
           </div>
 
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           <div class="col-md-2">
 
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               <embed src="images/notebook/protocols/lrha/1._Electrocompetent_cells_protocol_optimized_for_L._rhamnosus_GG.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
 +
               <a href="https://static.igem.org/mediawiki/2018/8/81/T--TecMonterrey_GDL--Protocols--1._Electrocompetent_cells_protocol_optimized_for_L._rhamnosus_GG.pdf" download="MediumPreparation">Electrocompetent cells protocol optimized for L. rhamnosus GG</a>
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            </center>
 
           </div>
 
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               <embed src="images/notebook/protocols/lrha/2._Electroporation_of_L._rhamnosus_GG.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--2._Electroporation_of_L._rhamnosus_GG.pdf" download="MediumPreparation">View Electroporation of L. rhamnosus GG.</a>
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            </center>
 
           </div>
 
           </div>
 
           <div class="col-md-2">
 
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               <embed src="images/notebook/protocols/lrha/Plasmid_isolation_for_Lactobacillus_spp.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/2/21/T--TecMonterrey_GDL--Protocols--Plasmid_isolation_for_Lactobacillus_spp.pdf" download="MediumPreparation">View Plasmid isolation for Lacatobacillus spp.</a>
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            </center>
 
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               <embed src="images/notebook/protocols/lrha/Total_DNA_extraction_L._rhamnosus_GG_by_kit.pdf" type="application/pdf" width="100%" height="400px" />
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            <center>
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               <a href="https://static.igem.org/mediawiki/2018/1/19/T--TecMonterrey_GDL--Protocols--Total_DNA_extraction_L._rhamnosus_GG_by_kit.pdf" download="MediumPreparation">View Total DNA extraction L. rhamnosus GG</a>
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            </center>
 
           </div>
 
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           <div class="col-md-2">
 
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Revision as of 00:39, 18 October 2018

TecMonterrey_GDL

Protocols

For E.coli :



View Preparation of LB Medium
View LB medium and LB agar-plates Protocol
View Competent cells


View Transformation by electroporation
View Calcio competent cells
View Ligation and digestion of G-Blocks


View Transformation Heat Shock
View MINIPREP KIT
View PCR


View Agarose Gel Electrophoresis
View Optimizing Restriction Endonuclease Reactions
View Protein extraction and purification based on Thermo Fisher's Protocol


View SDS-PAGE
View Western Blot based on Invitrogen's protocol


For Peptides :



View Competent cells
View Transformation by electroporation
View Calcio competent cells


View Procedure of digestion of the constructo
View High Fidelity restriction enzyme Protocol
View MINIPREP KIT


View PCR
View Protein Expression with T7 Strains


For L. rhamnosus GG :



Electrocompetent cells protocol optimized for L. rhamnosus GG
View Electroporation of L. rhamnosus GG.


View Plasmid isolation for Lacatobacillus spp.
View Total DNA extraction L. rhamnosus GG

TecMonterrey_GDL