Difference between revisions of "Team:TecMonterrey GDL/Protocols"
| Line 138: | Line 138: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| − | < | + | <ul> |
| − | <a href="https://static.igem.org/mediawiki/2018/2/26/T--TecMonterrey_GDL--Protocols--1._LB_medium_preparation.pdf">View Preparation of LB Medium</a> | + | <li> |
| − | + | <center> | |
| + | <a href="https://static.igem.org/mediawiki/2018/2/26/T--TecMonterrey_GDL--Protocols--1._LB_medium_preparation.pdf">View Preparation of LB Medium</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Protocols--3._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/a/a3/T--TecMonterrey_GDL--Protocols--3.3_Ligation_by_iGEM.pdf">View Ligation and digestion of G-Blocks</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/1/13/T--TecMonterrey_GDL--Protocols--3.4_Transformation_heat_shock_protocol.pdf">View Transformation Heat Shock</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| − | < | + | <ul> |
| − | <a href="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Protocols--1.1_LB_medium_and_LB_agar-plates_protocol.pdf">View LB medium and LB agar-plates Protocol</a> | + | <li> |
| − | + | <center> | |
| + | <a href="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Protocols--1.1_LB_medium_and_LB_agar-plates_protocol.pdf">View LB medium and LB agar-plates Protocol</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/c/c4/T--TecMonterrey_GDL--Protocols--4._Miniprep_kit.pdf">View MINIPREP KIT</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/9/99/T--TecMonterrey_GDL--Protocols--5._PCR.pdf">View PCR</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/0/0b/T--TecMonterrey_GDL--Protocols--5._Horizontal_electroforesis_protocol.pdf">View Agarose Gel Electrophoresis</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--5._Optimizing_restriction_EcoR_I_y_Spe_I.pdf">View Optimizing Restriction Endonuclease Reactions</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| − | < | + | <ul> |
| − | < | + | <li> |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/c/c0/T--TecMonterrey_GDL--Protocols--2._Electro_competent_cells_protocol.pdf">View Competent cells</a> | |
| − | + | </center> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/4/4d/T--TecMonterrey_GDL--Protocols--6._Lysis_and_protein_purification.pdf">View Protein extraction and purification based on Thermo Fisher's Protocol</a> | |
| − | + | </center> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/6/68/T--TecMonterrey_GDL--Protocols--7._SDS-PAGE_%2B_coomassie_staining_protocols.pdf">View SDS-PAGE</a> | |
| − | + | </center> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/0/0e/T--TecMonterrey_GDL--Protocols--8._Western_blot.pdf">View Western Blot based on Invitrogen's protocol</a> | |
| − | < | + | </center> |
| − | + | </li> | |
| − | + | </ul> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 231: | Line 227: | ||
<div class="row"> | <div class="row"> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| − | < | + | <ul> |
| − | <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._Electro_competent_cells_protocol.pdf">View Competent cells</a> | + | <li> |
| − | + | <center> | |
| + | <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._Electro_competent_cells_protocol.pdf">View Competent cells</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Protocols--Digestion_and_ligation.pdf">View Procedure of digestion of the constructo</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/3/34/T--TecMonterrey_GDL--Protocols-PCR.pdf">View PCR</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| − | < | + | <ul> |
| − | <a href="https://static.igem.org/mediawiki/2018/d/d8/T--TecMonterrey_GDL--Protocols--2._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | + | <li> |
| − | + | <center> | |
| + | <a href="https://static.igem.org/mediawiki/2018/d/d8/T--TecMonterrey_GDL--Protocols--2._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/7/75/T--TecMonterrey_GDL--Protocols--High_fidelity_restriction_enzymes_protocol.pdf">View High Fidelity restriction enzyme Protocol</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/1/1d/T--TecMonterrey_GDL--Protocols--Miniprep.pdf">View MINIPREP KIT</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
<div class="col-md-4"> | <div class="col-md-4"> | ||
| − | < | + | <ul> |
| − | <a href="https://static.igem.org/mediawiki/2018/1/14/T--TecMonterrey_GDL--Protocols--3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | + | <li> |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/1/14/T--TecMonterrey_GDL--Protocols--3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | |
| − | + | </center> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/6/65/T--TecMonterrey_GDL--Protocols--Protein_expression_with_T7_strains.pdf">View Protein Expression with T7 Strains</a> | |
| − | < | + | </center> |
| − | + | </li> | |
| − | + | </ul> | |
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
</div> | </div> | ||
| Line 287: | Line 285: | ||
<br><br> | <br><br> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col-md- | + | <div class="col-md-6"> |
| − | + | <ul> | |
| − | + | <li> | |
| − | + | <center> | |
| − | + | <a href="https://static.igem.org/mediawiki/2018/8/81/T--TecMonterrey_GDL--Protocols--1._Electrocompetent_cells_protocol_optimized_for_L._rhamnosus_GG.pdf">Electrocompetent cells protocol optimized for L. rhamnosus GG</a> | |
| − | + | </center> | |
| − | + | </li> | |
| − | + | <li> | |
| − | + | <center> | |
| − | < | + | <a href="https://static.igem.org/mediawiki/2018/2/21/T--TecMonterrey_GDL--Protocols--Plasmid_isolation_for_Lactobacillus_spp.pdf">View Plasmid isolation for Lacatobacillus spp.</a> |
| − | + | </center> | |
| − | + | </li> | |
| − | + | </ul> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | </ | + | |
</div> | </div> | ||
| − | <div class="col-md-2"> | + | <div class="col-md-6"> |
| + | <ul> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--2._Electroporation_of_L._rhamnosus_GG.pdf">View Electroporation of L. rhamnosus GG.</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | <li> | ||
| + | <center> | ||
| + | <a href="https://static.igem.org/mediawiki/2018/1/19/T--TecMonterrey_GDL--Protocols--Total_DNA_extraction_L._rhamnosus_GG_by_kit.pdf">View Total DNA extraction L. rhamnosus GG</a> | ||
| + | </center> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | </div> | ||
</div> | </div> | ||
</section> | </section> | ||
Revision as of 01:15, 18 October 2018