Difference between revisions of "Team:TecMonterrey GDL/Protocols"
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Revision as of 01:17, 18 October 2018
TecMonterrey_GDL
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Protocols
For
E.coli
:
View Preparation of LB Medium
View Transformation by electroporation
View Calcio competent cells
View Ligation and digestion of G-Blocks
View Transformation Heat Shock
View LB medium and LB agar-plates Protocol
View MINIPREP KIT
View PCR
View Agarose Gel Electrophoresis
View Optimizing Restriction Endonuclease Reactions
View Competent cells
View Protein extraction and purification based on Thermo Fisher's Protocol
View SDS-PAGE
View Western Blot based on Invitrogen's protocol
For
Peptides
:
View Competent cells
View Procedure of digestion of the constructo
View PCR
View Transformation by electroporation
View High Fidelity restriction enzyme Protocol
View MINIPREP KIT
View Calcio competent cells
View Protein Expression with T7 Strains
For
L. rhamnosus GG
:
Electrocompetent cells protocol optimized for L. rhamnosus GG
View Plasmid isolation for Lacatobacillus spp.
View Electroporation of L. rhamnosus GG.
View Total DNA extraction L. rhamnosus GG