Difference between revisions of "Team:TecMonterrey GDL/Protocols"

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                    <li><a href="https://2018.igem.org/Team:TecMonterrey_GDL/Protocols">Protocols</a></li>
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            <h1>Protocols</h1>
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<section class="service-overview section">
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      <div class="section-title">
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          <h3>For <i>E.coli</i> :</h3>
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      </div>
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      <br><br>
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        <div class="row">
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/1._LB_medium_preparation.pdf" type="application/pdf" width="100%" height="400px" />
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/1.1_LB_medium_and_LB_agar-plates_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/2._Electro_competent_cells_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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        <br><br>
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              <embed src="images/notebook/protocols/ecoli/3._Transformation_by_electroporation.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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              <embed src="images/notebook/protocols/ecoli/3.1_Competent_cells_BL21.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/3.3_Ligation_by_iGEM.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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        <br><br>
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              <embed src="images/notebook/protocols/ecoli/3.4_Transformation_heat_shock_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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              <embed src="images/notebook/protocols/ecoli/4._Miniprep_kit.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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              <embed src="images/notebook/protocols/ecoli/5._PCR.pdf" type="application/pdf" width="100%" height="400px" />
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        <br><br>
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              <embed src="images/notebook/protocols/ecoli/5._Horizontal_electroforesis_protocol.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/5._Optimizing_restriction_EcoR_I_y_Spe_I.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/6._Lysis_and_protein_purification.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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        <br><br>
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        <div class="row">
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              <embed src="images/notebook/protocols/ecoli/7._SDS-PAGE_+_coomassie_staining_protocols.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/ecoli/8._Western_blot.pdf" type="application/pdf" width="100%" height="400px" />
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          </div>
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        </div>
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        <br><br>
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        <div class="section-title">
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            <h3>For <i>Peptides</i> :</h3>
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        </div>
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        <br><br>
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        <div class="row">
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/peptides/1._Electro_competent_cells_protocol.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/peptides/2._Transformation_by_electroporation.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
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          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/peptides/3.1_Competent_cells_BL21.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
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        </div>
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        <br><br>
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        <div class="row">
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              <embed src="images/notebook/protocols/peptides/Digestion_and_ligation.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/peptides/High_fidelity_restriction_enzymes_protocol.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/peptides/Miniprep.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
        </div>
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        <br><br>
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        <div class="row">
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          <div class="col-md-2">
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          </div>
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          <div class="col-md-4">
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              <embed src="images/notebook/protocols/peptides/PCR.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/peptides/Protein_expression_with_T7_strains.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
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          <div class="col-md-2">
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          </div>
 +
        </div>
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        <br><br>
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        <div class="section-title">
 +
            <h3>For <i>L. rhamnosus GG</i> :</h3>
 +
        </div>
 +
        <br><br>
 +
        <div class="row">
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          <div class="col-md-2">
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          </div>
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          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/lrha/1._Electrocompetent_cells_protocol_optimized_for_L._rhamnosus_GG.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/lrha/2._Electroporation_of_L._rhamnosus_GG.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-2">
 +
          </div>
 +
        </div>
 +
        <br><br>
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        <div class="row">
 +
          <div class="col-md-2">
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          </div>
 +
          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/lrha/Plasmid_isolation_for_Lactobacillus_spp.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-4">
 +
              <embed src="images/notebook/protocols/lrha/Total_DNA_extraction_L._rhamnosus_GG_by_kit.pdf" type="application/pdf" width="100%" height="400px" />
 +
          </div>
 +
          <div class="col-md-2">
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Revision as of 09:24, 17 October 2018

TecMonterrey_GDL

Protocols

For E.coli :













For Peptides :









For L. rhamnosus GG :





TecMonterrey_GDL