Difference between revisions of "Team:CCU Taiwan/Judge"

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<div class="content">
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<p class="first">Background</p>
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                <p class="description">All of the 2018 iGEM teams are invited and encouraged to participate in the Fifth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which aimed to be one of the labs to make the calibration in different condition. In our case, we measured fluorescence using plate reader.</p>
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    <p class="first">Interlab Project</p>
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      <p><a href="#ca1">OD<sub>600</sub> Reference point - LUDOX Protocol</a></p>
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      <p><a href="#ca2">Particle Standard Curve - Microsphere Protocol</a></p>
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      <p><a href="#ca3">Fluorescence standard curve - Fluorescein Protoco</a></p>
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      <p><a href="#ca4">Cell measurement protocol</a></p>
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      <p><a href="#ca5">Colony Forming Units per 0.1 OD<sub>600</sub> E. coli cultures</a></p>
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                <p class="first">Experiment</p>
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                <p class="second" id="ca1">Calibration 1: OD<sub>600</sub> Reference Point - LUDOX Protocol</p>
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                <p class="second">Materials：</p>
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                <p class="description"><ol><li> 1ml LUDOX CL-X (provided in kit)</li><li> dd H<sub>2</sub>O (provided by team)</li><li> 96 well plate, black with clear flat bottom preferred (provided by team)</li></ol></p>
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Revision as of 04:16, 6 September 2018

Background

All of the 2018 iGEM teams are invited and encouraged to participate in the Fifth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which aimed to be one of the labs to make the calibration in different condition. In our case, we measured fluorescence using plate reader.

Interlab Project

OD600 Reference point - LUDOX Protocol

Particle Standard Curve - Microsphere Protocol

Fluorescence standard curve - Fluorescein Protoco

Cell measurement protocol

Colony Forming Units per 0.1 OD600 E. coli cultures

Experiment

Calibration 1: OD600 Reference Point - LUDOX Protocol

Materials：

  1. 1ml LUDOX CL-X (provided in kit)
  2. dd H2O (provided by team)
  3. 96 well plate, black with clear flat bottom preferred (provided by team)