Difference between revisions of "Team:CCU Taiwan/Model"

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                     <li class="title" id="Home"><a>Home</a>
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                         <ul class="sub" id="sub_home">
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<div id="home_word">
 
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<p>There's a home for us, always.</p>
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<a>There's a home for us, always.</a>
 
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                     <li class="title" id="Project"><a href="#">Project</a>
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                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr>
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<ul class="sub" id="sub_project" style="cursor:default;">
 
    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
 
<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
 
<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
 
<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
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<li class="list" id="project4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements">Achievements</a></li>
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<li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
 
<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
 
<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
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    <li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
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                     <li class="title" id="Parts"><a href="#">Parts</a>
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                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr>
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    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
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    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Overview</a></li>
 
<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
 
<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
 
<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
 
<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
 
<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
 
<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
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                         </ul>
 
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                     <li class="title" id="Modeling"><a href="#">Modeling</a>
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                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr>
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                             <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Model</a></li>
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                             <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Overview</a></li>
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<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Monolignol">Monolignol Binding</a></li>
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<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Lignin">Lignin Model</a></li>
 
<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
 
<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
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                             <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Our Plan</a></li>
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                             <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Overview</a></li>
 
<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
 
<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
 
<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
 
<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
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<hr>
 
                         </ul>
 
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<li class="title" id="Human_Practice"><a href="#">Human Practice</a>
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr>
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                             <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices">Overview</a></li>
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<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Engagement</a></li>
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<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Public</a></li>
 
<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
 
<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
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<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Business_Model">Business Model</a></li>
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<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate">Intergrate</a></li>
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<li class="title" id="Notebook"><a href="#">Notebook</a>
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                             <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Overview</a></li>
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<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design">Applied Design</a></li>
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<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations">Collaborations</a></li>
 
<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
 
<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
 
<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
 
<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
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<hr>
 
                         </ul>
 
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<!--<div class="scroll-line"></div>
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<div class="scroll-line-bg"></div>
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<div class="Quick-navigation">
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  <a href="#A" class="Quick-navigation-item">____Background</a>
 +
  <a href="#B" class="Quick-navigation-item">____Calibration 1</a>
 +
  <a href="#C" class="Quick-navigation-item">____Calibration 2</a>
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  <a href="#D" class="Quick-navigation-item">____Calibration 3</a>
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  <a href="#E" class="Quick-navigation-item">____Calibration 4</a>
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  <a href="#F" class="Quick-navigation-item">____Calibration 5</a>
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            <p class="first">Model </p>
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      <div class="content">
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            <p class="description">In order to realize the lignin-laminated paper cup, it is necessary to understand the properties (e.g., acid and alkali resistance, tensile strength, hardness, water absorption, etc.) of the laminating material, which will affect the parts used in the paper cup. Meanwhile, some techniques (e.g., FTIR, MALDI, SEM, etc.) are used to understand the microscopic structure. </p>
+
<p class="first">Modeling Overview</p>
−
            <p class="description">Since the lignin we use is not refined from trees, we will examine the film made between natural materials and our gene transferred yeast. The result can help us understand the properties impacted due to the differences between structures then can be applied on further product improvement. </p>
+
<p class="description">In the future, there will be fewer and fewer petrochemical resources on the planet, and plastic waste will accumulate. When things change, what should we do? To solve the problem, we use Liggreen. We hope to establish a new production line of laminating coating through biological methods. First of all, we use natural enzyme to synthesize the material. Through the synthetic reaction of enzyme and monolignols, we make our Liggreen structure be close to natural vegetation. Then, we could create a biological coating which will not require petrochemicals and high energy consumption. Our modeling is mainly divided into three parts to prove our project – Enzyme production, Monomer polymerization, Molecular prediction.</p>
−
            <p class="first">Device (production line) </p>
+
<p class="second">Enzyme production:</p>
−
            <p class="description">In order to use lignin on the lamination technology, the properties required for laminating are studied in the model. However, in the production of paper cups, it is impossible that only relying on the production in the laboratory. To solve the problem, we simulate a set of production lines for lignin film by researching lignin properties and visiting laminating industry. </p>
+
<p class="description">Transforming p.pastoris by synthetic biology. We want to make yeast produce the required enzymes – Lac1, Px16, Px18<br>Modeling: Proving the experiment by measuring the growth curve of p. pastoris and auto selection. Then using the different tag genes to design for western blot, proving the success of our overall enzyme production system.</p>
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<p class="second">Monomer polymerization:</p>
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<p class="description"> The three enzymes are added into the monolignols. And the monolignols will generate free radicals and bond to each other.<br>Modeling: We use Spartan to simulate the monolignol and the primary product, and then the free energy is obtained. The experiment was used to verify the spontaneity of the reaction.</p>
 +
<p class="second">Molecular prediction: </p>
 +
<p class="description">When the polymerization reaction is carried out to a certain extent, we would measure the physicochemical properties and carry out the modification. So that lignin can be better used in the coating industry.<br>Modeling: In this part, because we can't complete the all of polymerization reaction, we refer to paper for the estimation of molecular polymerization products. The estimation is mainly based on three parts – monomer, bond and product molecular weight. Since we mainly use the monolignol G for the reaction first, the structural estimation is mainly based on the Epoxy Resin.</p>
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Revision as of 10:01, 14 September 2018

Modeling Overview

In the future, there will be fewer and fewer petrochemical resources on the planet, and plastic waste will accumulate. When things change, what should we do? To solve the problem, we use Liggreen. We hope to establish a new production line of laminating coating through biological methods. First of all, we use natural enzyme to synthesize the material. Through the synthetic reaction of enzyme and monolignols, we make our Liggreen structure be close to natural vegetation. Then, we could create a biological coating which will not require petrochemicals and high energy consumption. Our modeling is mainly divided into three parts to prove our project – Enzyme production, Monomer polymerization, Molecular prediction.

Enzyme production:

Transforming p.pastoris by synthetic biology. We want to make yeast produce the required enzymes – Lac1, Px16, Px18
Modeling: Proving the experiment by measuring the growth curve of p. pastoris and auto selection. Then using the different tag genes to design for western blot, proving the success of our overall enzyme production system.

Monomer polymerization:

The three enzymes are added into the monolignols. And the monolignols will generate free radicals and bond to each other.
Modeling: We use Spartan to simulate the monolignol and the primary product, and then the free energy is obtained. The experiment was used to verify the spontaneity of the reaction.

Molecular prediction:

When the polymerization reaction is carried out to a certain extent, we would measure the physicochemical properties and carry out the modification. So that lignin can be better used in the coating industry.
Modeling: In this part, because we can't complete the all of polymerization reaction, we refer to paper for the estimation of molecular polymerization products. The estimation is mainly based on three parts – monomer, bond and product molecular weight. Since we mainly use the monolignol G for the reaction first, the structural estimation is mainly based on the Epoxy Resin.