Difference between revisions of "Team:UCSC/Attributions"

The PoPPY team would like to thank everyone who made this project possible, especially those described below. For the past five years, undergraduates at UCSC have participated in iGEM. This year, the team is composed of students studying Bioengineering (Biomolecular), Bioinformatics, Bioengineering (Assistive Technology: Motor), Molecular, Cellular, and Developmental Biology, Marine Biology, and Cognitive Science: Artificial Intelligence and Human Computer Interaction. Our diverse group of individuals is crucial for the creative process behind using synthetic biology to help others. Modern engineering demands collaboration between individuals and pushes them to think critically and work efficiently. Through synthetic biology, the 2018 UCSC iGEM team is solving worldwide issues and developing methods to expand their research in the future.

The Project Idea

After several journal club meetings, our team determined the PoPPY project was the most meaningful pursuit. The initial idea was proposed by captain Morgan Tardy and mentor McKenna Hicks.

Official Team

Thank you to all the 2018 UCSC iGEM Team members for their hard work and dedication to the PoPPY project's message. Check out our Meet the Team page to learn about each teammate.

Advisors

Dr. David Bernick, Assistant Adjunct Professor, University of California Santa Cruz, Department of Biomolecular Engineering

Thank you to Dr. Bernick for guiding our team and offering valuable insight and advice through each stage of the project. He helped our team manage funding, finances and logistical tasks.

Mckenna Hicks, Master's Student, University of California Santa Cruz, Department of Biomolecular Engineering

Thank you to McKenna for providing advice on gene design and wet lab experiments.

Research Support

Tony Carr, Professor of Molecular Genetics, University of Sussex, Genome Damage and Stability, Genome Damage and Stability Centre

Thank you for answering our questions regarding the size of genomic insert, directionality of Lox sites, and cre-lox recombination.

Stefan Tippmann, Visiting Researcher, Chalmers University, Life Science Engineering

Thank you for teaching us about yeast in general and providing us with additional readings to learn about Yarrowia lipolytica.

Oliver Konzock, PhD Student, Chalmers University, Life Science Engineering

Answered questions general questions on how to grow yeast and yarrowia lipolytica. Answered questions on how to do yeast mediated cloning.

Jean-Marc Nicaud, Directeur de Recherche INRA

Introduced us to Golden Gate Assembly method. Answered questions building plasmids. Showed us M1G1 wouldn't be an issue.

Adam Watson, Research Fellow, University of Sussex, Genome Damage and Stability

Answered questions regarding size of genomic insert, directionality of lox sites, and cre-lox in general.

Erin Bredeweg, Biosystems Dynamics & Simula Scientist, Pacific Northwest National Laboratory

Assisted in location and finding sequence information of Yarrowia strain.

Catherine Duport, University of Avignon, Microbiotechnology, Ecotoxicology, and Bioremediation

Helped clarify pieces of experiment such as why she used certain promoters. Attempted to find yarrowia strain.

Saumya Ramarao, Senior Associate, Reproductive Health Supplies Coalition

Provided information regarding birth control production and needs.

Hal Alper, Professor, The University of Texas at Austin, Department of Chemical Engineering

Provided recommendations for Tsynth27 terminator and confirmed which promoters would be necessary for our project.

William Belden, Associate Professor, Rutgers University, Department of Animal Sciences

Provided information on yeast-mediated cloning experiment. Offered to help design flanking oligos.

Peng Xu, University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering

Proved Yarrowia Lipolytica could produce progesterone despite NHEJ preference. Showed that it is possible to use homologous recombination with Ku70 knockout Yarrowia Lipolytica strain.

Wendy Sun, University of Maryland Baltimore County, Department of Chemical Biochemical and Environmental Engineering

Proved Yarrowia Lipolytica could produce progesterone despite NHEJ preference. Showed that it is possible to use homologous recombination with Ku70 knockout Yarrowia Lipolytica strain.

Marc Gartenberg, Professor, Rutgers University, Department of Biochemistry and Molecular Biology

Provided information on parts of pXRU3/L2 plasmid including HOp region, CEN/ARS regions, pRS marker, and how to insert lox sites into these particular plasmids.

Daniel Gallie, Professor, University of California Riverside, Biochemistry Department

Sent electroporation protocol with mRNA for reference.

Ian wheeldon, Associate Professor, University of California Riverside, Department of Chemical and Environmental Engineering

Provided pHR_AO8_hrGFP plasmid sequence including homologous arm regions.

Cory Schwartz, PhD Student, University of California Riverside, Department of Civil and Environmental Engineering

Provided pHR_AO8_hrGFP plasmid sequence including homologous arm regions.

Hans Ronne, Professor, Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology

Helped attempt to determine how to stop the binding of Mig1 to Gal1 promoter.

Jah Nehlin, Copenhagen University Hospital Hvidovre

Gave insight into how Gal1 promoter works.

Rohinton Kamakaka, Professor, University of California Santa Cruz, Department of Molecular Cellular and Developmental Biology

Sent protocol on yeast mediated cloning. Clarified questions on yeast and may provide Saccharomyces Cerevisiae strain.

Lab Support

Dr. Hugh Olsen, Lab Manager, University of California, Santa Cruz, Biomolecular Engineering Teaching Labs

Provided assistance with autoclave-training, general laboratory safety training, obtaining materials and maintaining equipment.

Wiki Support

Evan Pepper, Former Undergraduate Student, University of California Santa Cruz, Biomolecular Engineering

Provided advice for design choices and taking criticism regarding said design choices.

UCSC iGEM 2017

For providing us with inspiration for the UCSC 2018 wiki and, more specifically, a layout for the Attributions page.

Human Practices Support

Funding Support

UCSC iGEM 2017

Provide monetary support for our team.

Industry Support

Fungal Genetics Stock Center,

Provided the team with Yarrowia Lipolytica Strain.

Asana,

Provided team with free premium accounts to organize and communicate work ideas with each other.

Non-Profit Partnerships

Difficult Technique Support

Presentation Support

General Support

Russell Evans, Senior Development Engineer, University of California, Santa Cruz, Campus Facilities

Provided the 2018 UCSC iGEM Team with separate dry lab research and wet lab rooms. Provided technical and equipment support.

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-   Answered questions regarding size of genomic insert, directionality of lox sites, and cre-lox.
+   Thank you for answering our questions regarding the size of genomic insert, directionality of Lox sites, and cre-lox recombination.
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-  Clarified Questions on yeast in general and provided us with additional readings to look through.
+ Thank you for teaching us about yeast in general and providing us with additional readings to learn about <i>Yarrowia lipolytica</i>.
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